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The map label for this gene is ansB [H]

Identifier: 152969719

GI number: 152969719

Start: 1318257

End: 1319261

Strand: Direct

Name: ansB [H]

Synonym: KPN_01165

Alternate gene names: 152969719

Gene position: 1318257-1319261 (Clockwise)

Preceding gene: 152969716

Following gene: 152969721

Centisome position: 24.8

GC content: 59.3

Gene sequence:

>1005_bases
ATGAGCAGCCTGGCGTTTAGCGAGACGCGTCTTCCGCATATTGTTATTTTAGCCACCGGCGGAACGATTGCTGGTTCGGC
GGCCAGCAATACCCAAACAACCGGCTATAAAGCCGGGGCGCTTGGGGTGCAGACGCTGATTAACGCCGTGCCGGAAATGA
GTAAAATCGCTCACGTCGAGGGCGAGCAGGTGGCCAATATTGGCAGTGAGAATATGACCAGCGATATTATTCTCCAGCTC
TCGAAGCGGGTAAATGCGCTATTGGCCCGGGACGATGTCGATGGCGTGGTGATCACCCATGGCACGGACACGCTGGATGA
AACCCCGTATTTCCTCAATCTGACGGTGAAAAGCAACAAGCCGGTGGTCTTTACCGCGGCGATGCGCCCGGCGACGGCGA
TCAGCGCCGACGGGCCGATGAACCTGCTGGAAGCGGTCACGGTCGCCGCCGATCCTGATGCCCGGGGACGCGGGGTAATG
GTGGTGCTTAACGACCGCATCGGCGCGGCGCGCTTTGTCACCAAAACTAATGCCACTTCGCTGGATACCTTCCGGGCGCC
CGAGGAGGGCTATCTTGGTGTCGTGGTGGGCGGAAAACCCCAGTTCGAGACGCGGGTGGATAAAATTCACACGCTGCGCT
CGGTGTTCGATGTGCGTCAGCTGAAGGTTCTGCCGAAGGTGGTGATTATTTATGGTTACCAGGACGATCCGGAGTATATG
TACGATGCGGCGATCGCCCATCATGCCGACGGTATTATCTACGCCGGAACCGGGGCGGGGTCGGTGTCGGTACGTAGCGC
CGCCGGTATCAAAAAAGCGCAGCAGGCGGGCATCGTGGTGGTGCGAGCCTCCCGTACCGGCAGCGGCGTCGTACCGCCGG
ATGACAGCCAGCCCGGGCTGGTGGCCGACTCCCTCAACCCGGCAAAAGCGCGTATACTGCTGATGACCGCGTTGACGCAG
ACTAAAGATCCGCAGCTGATTCAGCAATATTTCCATACCTACTGA

Upstream 100 bases:

>100_bases
GCAAGCCCTTTGCATTAAGCAAGCGTAAGTTTGATTAATGTCTATGAAGGGAGTGACCTGTATGAATTTTAGAGCATTAC
TTGCCATTGCGTTATTGACG

Downstream 100 bases:

>100_bases
TCCTCTGCGGCGAGGGCGTTGTTCACACCCTCGCCGTGCTTTCTTCTCAATGACCTGGCTTCCATGCAAATCGATGGCGA
ATGATTGCCCATCATTCACT

Product: putative asparaginase/glutaminase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSSLAFSETRLPHIVILATGGTIAGSAASNTQTTGYKAGALGVQTLINAVPEMSKIAHVEGEQVANIGSENMTSDIILQL
SKRVNALLARDDVDGVVITHGTDTLDETPYFLNLTVKSNKPVVFTAAMRPATAISADGPMNLLEAVTVAADPDARGRGVM
VVLNDRIGAARFVTKTNATSLDTFRAPEEGYLGVVVGGKPQFETRVDKIHTLRSVFDVRQLKVLPKVVIIYGYQDDPEYM
YDAAIAHHADGIIYAGTGAGSVSVRSAAGIKKAQQAGIVVVRASRTGSGVVPPDDSQPGLVADSLNPAKARILLMTALTQ
TKDPQLIQQYFHTY

Sequences:

>Translated_334_residues
MSSLAFSETRLPHIVILATGGTIAGSAASNTQTTGYKAGALGVQTLINAVPEMSKIAHVEGEQVANIGSENMTSDIILQL
SKRVNALLARDDVDGVVITHGTDTLDETPYFLNLTVKSNKPVVFTAAMRPATAISADGPMNLLEAVTVAADPDARGRGVM
VVLNDRIGAARFVTKTNATSLDTFRAPEEGYLGVVVGGKPQFETRVDKIHTLRSVFDVRQLKVLPKVVIIYGYQDDPEYM
YDAAIAHHADGIIYAGTGAGSVSVRSAAGIKKAQQAGIVVVRASRTGSGVVPPDDSQPGLVADSLNPAKARILLMTALTQ
TKDPQLIQQYFHTY
>Mature_333_residues
SSLAFSETRLPHIVILATGGTIAGSAASNTQTTGYKAGALGVQTLINAVPEMSKIAHVEGEQVANIGSENMTSDIILQLS
KRVNALLARDDVDGVVITHGTDTLDETPYFLNLTVKSNKPVVFTAAMRPATAISADGPMNLLEAVTVAADPDARGRGVMV
VLNDRIGAARFVTKTNATSLDTFRAPEEGYLGVVVGGKPQFETRVDKIHTLRSVFDVRQLKVLPKVVIIYGYQDDPEYMY
DAAIAHHADGIIYAGTGAGSVSVRSAAGIKKAQQAGIVVVRASRTGSGVVPPDDSQPGLVADSLNPAKARILLMTALTQT
KDPQLIQQYFHTY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=329, Percent_Identity=48.0243161094225, Blast_Score=266, Evalue=1e-72,
Organism=Escherichia coli, GI1788065, Length=178, Percent_Identity=29.2134831460674, Blast_Score=63, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323189, Length=316, Percent_Identity=43.6708860759494, Blast_Score=242, Evalue=7e-65,
Organism=Saccharomyces cerevisiae, GI6323187, Length=316, Percent_Identity=43.6708860759494, Blast_Score=242, Evalue=7e-65,
Organism=Saccharomyces cerevisiae, GI6323186, Length=316, Percent_Identity=43.6708860759494, Blast_Score=242, Evalue=7e-65,
Organism=Saccharomyces cerevisiae, GI6323184, Length=316, Percent_Identity=43.6708860759494, Blast_Score=242, Evalue=7e-65,
Organism=Saccharomyces cerevisiae, GI6320527, Length=341, Percent_Identity=37.5366568914956, Blast_Score=218, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35372; Mature: 35241

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLAFSETRLPHIVILATGGTIAGSAASNTQTTGYKAGALGVQTLINAVPEMSKIAHVE
CCCCCCCCCCCCEEEEEEECCEEECCCCCCCCCCCEECCHHHHHHHHHHCCHHHHHHHCC
GEQVANIGSENMTSDIILQLSKRVNALLARDDVDGVVITHGTDTLDETPYFLNLTVKSNK
CHHHHCCCCCCCHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCCEEEEEEEECCC
PVVFTAAMRPATAISADGPMNLLEAVTVAADPDARGRGVMVVLNDRIGAARFVTKTNATS
CEEEEECCCCCEEECCCCCHHHHHHEEEECCCCCCCCEEEEEEECCCCCEEEEEECCCCC
LDTFRAPEEGYLGVVVGGKPQFETRVDKIHTLRSVFDVRQLKVLPKVVIIYGYQDDPEYM
CHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHH
YDAAIAHHADGIIYAGTGAGSVSVRSAAGIKKAQQAGIVVVRASRTGSGVVPPDDSQPGL
HHHHHHHCCCCEEEEECCCCCEEEHHHHCHHHHHHCCEEEEEECCCCCCCCCCCCCCCCE
VADSLNPAKARILLMTALTQTKDPQLIQQYFHTY
EECCCCCHHHEEEEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SSLAFSETRLPHIVILATGGTIAGSAASNTQTTGYKAGALGVQTLINAVPEMSKIAHVE
CCCCCCCCCCCEEEEEEECCEEECCCCCCCCCCCEECCHHHHHHHHHHCCHHHHHHHCC
GEQVANIGSENMTSDIILQLSKRVNALLARDDVDGVVITHGTDTLDETPYFLNLTVKSNK
CHHHHCCCCCCCHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCCEEEEEEEECCC
PVVFTAAMRPATAISADGPMNLLEAVTVAADPDARGRGVMVVLNDRIGAARFVTKTNATS
CEEEEECCCCCEEECCCCCHHHHHHEEEECCCCCCCCEEEEEEECCCCCEEEEEECCCCC
LDTFRAPEEGYLGVVVGGKPQFETRVDKIHTLRSVFDVRQLKVLPKVVIIYGYQDDPEYM
CHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHH
YDAAIAHHADGIIYAGTGAGSVSVRSAAGIKKAQQAGIVVVRASRTGSGVVPPDDSQPGL
HHHHHHHCCCCEEEEECCCCCEEEHHHHCHHHHHHCCEEEEEECCCCCCCCCCCCCCCCE
VADSLNPAKARILLMTALTQTKDPQLIQQYFHTY
EECCCCCHHHEEEEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3026924; 3194219; 3379033; 8348975 [H]