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The map label for this gene is alkK [H]

Identifier: 152969682

GI number: 152969682

Start: 1280633

End: 1282261

Strand: Direct

Name: alkK [H]

Synonym: KPN_01128

Alternate gene names: 152969682

Gene position: 1280633-1282261 (Clockwise)

Preceding gene: 152969681

Following gene: 152969687

Centisome position: 24.09

GC content: 63.41

Gene sequence:

>1629_bases
ATGCCGTCACCGATGATTTATCAGGATCTCACCACTGCCGCGCTGCTCGGCCATGCGGCGCAGTATCACAGCGAGACCGA
GATTGTCTCGGTGAGTACTGGCGGTGAGAAGGAGCGCAGCTGCTGGGGGGAGGTGGCCAGCCGTGCTCAGCGGCTGGCAT
CGGCGCTGGCGTCGCTGGGGCTGCCGCCGGGCGCCCGCTGCGCCACGCTGGCATGGAACAACCGCCGCCATCTGGAGATC
TATTTTGCCGTCGCCTCCGGCGGCTGGGTGACGCACACTGTCAATCCGCGGCTGTCGGTTGACCATCTGCGGTATATCCT
CAACGACGCTGCCGATGAAGTGCTGTTTTTTGACCAGACCTTTTTGCCGCTGGTAGCGCAGCTGCTGCCGCAGCTGCCGA
CGGTGAAACACGTCGTGCTGATGGAGTCCCGCAGCGAAGCGGCGCTCAGCCAGCTGCCCTCATTGCTGTTTTATGACGAT
CTGTTGCAGCAGGGGATGGCAGACTATCGCTGGCCGCAGCTTAATGAACTGACTCCGGCTTCGCTCTGTTACACCTCGGG
AACCACCGGCCGGCCGAAAGGGGTATTGAACACCCATCGTTCGCTGGTCCTGCACGCCCTGAGCGGCAACCAGCCGGACG
CCGCCGGCATATCGGCGAAGGACAGCCTGTTGCCGGTGGTGCCGATGTTCCATGTCAACGCGTGGGGGACGCCGTTTATC
GCCGCCATGGTCGGCTCGCGGCTGGTGCTGCCGGGGCCGCACCTCGATGGCGACAGCCTGCTGCAGTTGCTGGCCGCCGA
GAAGGTGACGGTGGGGTTTGGCGTGCCGGTGATCTGGGCCGGCTTGCTGGCGGCGATGCGCCGGGCAGAGGTCCGGCTGC
CTGAATTTAAACGTGCGCTGGTCGGCGGCTCCGCGCTGCCGCCGTCGATGGCCGAGGCCTTTCAGCGTGATTACGGCATT
GCGCTGACCCACGCCTGGGGAATGACCGAAACATCGCCCATCGGCACCATCAACACACCGTTGAGCAAACATGACGCGCT
CCCGGCACAGGAGCAGCAAAAGCAGTGCGCCGGTCAGGGGCGGCCCATTTTCGGCATCGAACTGCAGGTGGTGGACGTGG
ATGGCGAACCGTTGCCCCGGGATGGTCAAAGCCAGGGGTATCTGCAGGTCAGAGGGCACTGGGTGGTAGAGCAATACTAT
GGTCAGGACGCGAGCGCCCTGACCGCGGCAGGCTGGTTTGATACCGGTGATATCGGTACGCTCGACGCCAATGGTTATCT
GGTGATCAGCGATCGGGCAAAGGATATTATCAAGTCCGGTGGCGAGTGGATCTCCACCGTTGAGCTGGAGAATATCGCCA
TCGCGCATCCGGGGGTGCGCAGCGCAGCGGCCATCGCCGCCCGCCATCCGCGCTGGGACGAGCGGCCGGTGCTGCTGTGC
GTCCGTGCGGAAGGGGGCGAAGTGGAGGAGACCGATCTGCTGAGCTGGTTTGAGAAGCGGGTGCCTAAATGGCAGATCCC
GGACCGGGTGATATTTGTCGATGCGCTGCCGGTCAGCGCCACGGGTAAGGTGCTCAAAAATCAGCTGCGCCAGGCCTACG
GAGAGATTTTAATGAGTGAGGGAAAATGA

Upstream 100 bases:

>100_bases
TTTTGTGCAGACCGGCAAATGTAGACCAGATTCAATAGGCGCCCGCACGATCGCGCCGCGCCCCTCGATTCCGCCGGACG
TCTGTAAAGGAGACCCCGCC

Downstream 100 bases:

>100_bases
GCCATGCGCGACCCGGTCTGACGACCTGTTCTCAGTATGATGCGGCCCTGCACGCCTTGTCGGCGGCGCGCCAGCGCTGG
GCGGAAACCTCAGTGAACCG

Product: crotonobetaine/carnitine-CoA ligase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MPSPMIYQDLTTAALLGHAAQYHSETEIVSVSTGGEKERSCWGEVASRAQRLASALASLGLPPGARCATLAWNNRRHLEI
YFAVASGGWVTHTVNPRLSVDHLRYILNDAADEVLFFDQTFLPLVAQLLPQLPTVKHVVLMESRSEAALSQLPSLLFYDD
LLQQGMADYRWPQLNELTPASLCYTSGTTGRPKGVLNTHRSLVLHALSGNQPDAAGISAKDSLLPVVPMFHVNAWGTPFI
AAMVGSRLVLPGPHLDGDSLLQLLAAEKVTVGFGVPVIWAGLLAAMRRAEVRLPEFKRALVGGSALPPSMAEAFQRDYGI
ALTHAWGMTETSPIGTINTPLSKHDALPAQEQQKQCAGQGRPIFGIELQVVDVDGEPLPRDGQSQGYLQVRGHWVVEQYY
GQDASALTAAGWFDTGDIGTLDANGYLVISDRAKDIIKSGGEWISTVELENIAIAHPGVRSAAAIAARHPRWDERPVLLC
VRAEGGEVEETDLLSWFEKRVPKWQIPDRVIFVDALPVSATGKVLKNQLRQAYGEILMSEGK

Sequences:

>Translated_542_residues
MPSPMIYQDLTTAALLGHAAQYHSETEIVSVSTGGEKERSCWGEVASRAQRLASALASLGLPPGARCATLAWNNRRHLEI
YFAVASGGWVTHTVNPRLSVDHLRYILNDAADEVLFFDQTFLPLVAQLLPQLPTVKHVVLMESRSEAALSQLPSLLFYDD
LLQQGMADYRWPQLNELTPASLCYTSGTTGRPKGVLNTHRSLVLHALSGNQPDAAGISAKDSLLPVVPMFHVNAWGTPFI
AAMVGSRLVLPGPHLDGDSLLQLLAAEKVTVGFGVPVIWAGLLAAMRRAEVRLPEFKRALVGGSALPPSMAEAFQRDYGI
ALTHAWGMTETSPIGTINTPLSKHDALPAQEQQKQCAGQGRPIFGIELQVVDVDGEPLPRDGQSQGYLQVRGHWVVEQYY
GQDASALTAAGWFDTGDIGTLDANGYLVISDRAKDIIKSGGEWISTVELENIAIAHPGVRSAAAIAARHPRWDERPVLLC
VRAEGGEVEETDLLSWFEKRVPKWQIPDRVIFVDALPVSATGKVLKNQLRQAYGEILMSEGK
>Mature_541_residues
PSPMIYQDLTTAALLGHAAQYHSETEIVSVSTGGEKERSCWGEVASRAQRLASALASLGLPPGARCATLAWNNRRHLEIY
FAVASGGWVTHTVNPRLSVDHLRYILNDAADEVLFFDQTFLPLVAQLLPQLPTVKHVVLMESRSEAALSQLPSLLFYDDL
LQQGMADYRWPQLNELTPASLCYTSGTTGRPKGVLNTHRSLVLHALSGNQPDAAGISAKDSLLPVVPMFHVNAWGTPFIA
AMVGSRLVLPGPHLDGDSLLQLLAAEKVTVGFGVPVIWAGLLAAMRRAEVRLPEFKRALVGGSALPPSMAEAFQRDYGIA
LTHAWGMTETSPIGTINTPLSKHDALPAQEQQKQCAGQGRPIFGIELQVVDVDGEPLPRDGQSQGYLQVRGHWVVEQYYG
QDASALTAAGWFDTGDIGTLDANGYLVISDRAKDIIKSGGEWISTVELENIAIAHPGVRSAAAIAARHPRWDERPVLLCV
RAEGGEVEETDLLSWFEKRVPKWQIPDRVIFVDALPVSATGKVLKNQLRQAYGEILMSEGK

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=530, Percent_Identity=25.2830188679245, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI187761345, Length=541, Percent_Identity=25.1386321626617, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI187761343, Length=541, Percent_Identity=25.1386321626617, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI38505220, Length=517, Percent_Identity=24.1779497098646, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI42544132, Length=364, Percent_Identity=23.9010989010989, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI115511026, Length=461, Percent_Identity=24.0780911062907, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI122937307, Length=522, Percent_Identity=21.6475095785441, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI28416953, Length=564, Percent_Identity=24.290780141844, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI157311624, Length=502, Percent_Identity=21.5139442231076, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI157311622, Length=502, Percent_Identity=21.5139442231076, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI58082049, Length=506, Percent_Identity=20.3557312252964, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI13375727, Length=407, Percent_Identity=24.5700245700246, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI221142682, Length=518, Percent_Identity=25.2895752895753, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1788107, Length=526, Percent_Identity=25.2851711026616, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI145693145, Length=365, Percent_Identity=29.8630136986301, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1786810, Length=547, Percent_Identity=26.8738574040219, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1790505, Length=440, Percent_Identity=24.0909090909091, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789201, Length=357, Percent_Identity=24.3697478991597, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788595, Length=496, Percent_Identity=26.008064516129, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786801, Length=334, Percent_Identity=27.2455089820359, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1786529, Length=553, Percent_Identity=22.7848101265823, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71994703, Length=531, Percent_Identity=25.4237288135593, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI71994694, Length=531, Percent_Identity=25.4237288135593, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI71994690, Length=531, Percent_Identity=25.4237288135593, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17558820, Length=537, Percent_Identity=24.9534450651769, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI32563687, Length=509, Percent_Identity=25.147347740668, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17560308, Length=543, Percent_Identity=22.4677716390424, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=536, Percent_Identity=23.8805970149254, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=536, Percent_Identity=21.6417910447761, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17559526, Length=531, Percent_Identity=24.4821092278719, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17538037, Length=509, Percent_Identity=22.9862475442043, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17560140, Length=464, Percent_Identity=23.4913793103448, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=367, Percent_Identity=24.2506811989101, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71996755, Length=499, Percent_Identity=21.2424849699399, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=371, Percent_Identity=25.8760107816712, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24581924, Length=567, Percent_Identity=26.4550264550265, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21355181, Length=488, Percent_Identity=24.7950819672131, Blast_Score=116, Evalue=5e-26,
Organism=Drosophila melanogaster, GI18859661, Length=362, Percent_Identity=25.6906077348066, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24648253, Length=526, Percent_Identity=22.2433460076046, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24648255, Length=527, Percent_Identity=22.3908918406072, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648260, Length=513, Percent_Identity=24.9512670565302, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21356947, Length=506, Percent_Identity=23.9130434782609, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24653035, Length=494, Percent_Identity=22.4696356275304, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI161076582, Length=502, Percent_Identity=22.5099601593625, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24667955, Length=392, Percent_Identity=26.0204081632653, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI62472339, Length=392, Percent_Identity=26.0204081632653, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24667959, Length=392, Percent_Identity=26.0204081632653, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI20130357, Length=367, Percent_Identity=25.0681198910082, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356441, Length=487, Percent_Identity=21.1498973305955, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21358303, Length=521, Percent_Identity=21.1132437619962, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24648257, Length=398, Percent_Identity=24.6231155778894, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI281365686, Length=258, Percent_Identity=24.4186046511628, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 58901; Mature: 58770

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPMIYQDLTTAALLGHAAQYHSETEIVSVSTGGEKERSCWGEVASRAQRLASALASLG
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
LPPGARCATLAWNNRRHLEIYFAVASGGWVTHTVNPRLSVDHLRYILNDAADEVLFFDQT
CCCCCCEEEEEECCCEEEEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHEEEECHH
FLPLVAQLLPQLPTVKHVVLMESRSEAALSQLPSLLFYDDLLQQGMADYRWPQLNELTPA
HHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCH
SLCYTSGTTGRPKGVLNTHRSLVLHALSGNQPDAAGISAKDSLLPVVPMFHVNAWGTPFI
HEEECCCCCCCCCCHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHH
AAMVGSRLVLPGPHLDGDSLLQLLAAEKVTVGFGVPVIWAGLLAAMRRAEVRLPEFKRAL
HHHHCCEEEECCCCCCHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHH
VGGSALPPSMAEAFQRDYGIALTHAWGMTETSPIGTINTPLSKHDALPAQEQQKQCAGQG
HCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHCCC
RPIFGIELQVVDVDGEPLPRDGQSQGYLQVRGHWVVEQYYGQDASALTAAGWFDTGDIGT
CCEEEEEEEEEECCCCCCCCCCCCCCEEEEECEEEEEHHHCCCCCCEEECCCCCCCCCEE
LDANGYLVISDRAKDIIKSGGEWISTVELENIAIAHPGVRSAAAIAARHPRWDERPVLLC
ECCCCEEEECCHHHHHHHCCCCEEEEEEECCEEEECCCCHHHHHHHHCCCCCCCCCEEEE
VRAEGGEVEETDLLSWFEKRVPKWQIPDRVIFVDALPVSATGKVLKNQLRQAYGEILMSE
EECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
GK
CC
>Mature Secondary Structure 
PSPMIYQDLTTAALLGHAAQYHSETEIVSVSTGGEKERSCWGEVASRAQRLASALASLG
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
LPPGARCATLAWNNRRHLEIYFAVASGGWVTHTVNPRLSVDHLRYILNDAADEVLFFDQT
CCCCCCEEEEEECCCEEEEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHEEEECHH
FLPLVAQLLPQLPTVKHVVLMESRSEAALSQLPSLLFYDDLLQQGMADYRWPQLNELTPA
HHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCH
SLCYTSGTTGRPKGVLNTHRSLVLHALSGNQPDAAGISAKDSLLPVVPMFHVNAWGTPFI
HEEECCCCCCCCCCHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHH
AAMVGSRLVLPGPHLDGDSLLQLLAAEKVTVGFGVPVIWAGLLAAMRRAEVRLPEFKRAL
HHHHCCEEEECCCCCCHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHH
VGGSALPPSMAEAFQRDYGIALTHAWGMTETSPIGTINTPLSKHDALPAQEQQKQCAGQG
HCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHCCC
RPIFGIELQVVDVDGEPLPRDGQSQGYLQVRGHWVVEQYYGQDASALTAAGWFDTGDIGT
CCEEEEEEEEEECCCCCCCCCCCCCCEEEEECEEEEEHHHCCCCCCEEECCCCCCCCCEE
LDANGYLVISDRAKDIIKSGGEWISTVELENIAIAHPGVRSAAAIAARHPRWDERPVLLC
ECCCCEEEECCHHHHHHHCCCCEEEEEEECCEEEECCCCHHHHHHHHCCCCCCCCCEEEE
VRAEGGEVEETDLLSWFEKRVPKWQIPDRVIFVDALPVSATGKVLKNQLRQAYGEILMSE
EECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
GK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]