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The map label for this gene is ywbI [H]

Identifier: 152969319

GI number: 152969319

Start: 846680

End: 847564

Strand: Direct

Name: ywbI [H]

Synonym: KPN_00762

Alternate gene names: 152969319

Gene position: 846680-847564 (Clockwise)

Preceding gene: 152969315

Following gene: 152969321

Centisome position: 15.93

GC content: 35.14

Gene sequence:

>885_bases
ATGGAAATTAGACAGCTGGAATATTTTATTAGCGCATCTGCATTAGGCAACCTTACCAAGGTTGCAGAACGTCATTATGT
TTCGCAACCAAATATAACAGTTGCTATAAAAAAGCTAGAGTCTGAGTTAGGTGTTAATCTGTTTGAGAGAAGGAAAAATA
AGTTGGTGTTAACGGAAGAAGGGGAATTCTTCTTATTAAAAGTCAAACCTCTCATTATTGCATTGAAGAATAGTGTTGCT
GAAATTAAAGATTATCATAATAAAAACAGTGGAGTGGTAACATTAGGGATTCCTCCTATGATTAGCCTTTTTTTGTTTAC
ACCACTATTTAAACATTTTCGCGAATTATATTCAGAAATGGAACTTTCATTAGTTGAGGAAGGTACCTTTGGGTTGCATC
AAAAACTTTCTCATGGCGATATTGATTTGGCGATTGTCATTATCAATGATGCACCAGAAGAGTTAGTAACTGTACCATTA
ATGACTCAGCAGCATGTTGTATGTATATCCGATAAGCACCCTTTGGCGAAGAAGAAAACTATCGACTGGGAGGATTTGCA
AAATGAACCATTAATTGTTATGAAAAAAGATTCATGGCATCGTAAAGCTATTCTAAATGAATGTGAAAAGAGGGGTGTTA
ATACTCATATCTTCTTATCTTCCAATAGTGTAAAGAATAATATTGATTTGGTGGCAAAAAATGAGGGTATATCATTTATT
CTCGATGCGGTAGGGCTAAAATGTGATGGTGTAACAACAAGAGCGATGACAGAACCTGTCTATGTCGAGATAGGCCTGGC
ATGGAAAAAGGATAAGTATTTATCATACGCATCAAGAGCATTAATTGCATTCCTGGAACAGTATATTAAAGACAATTTCC
AATAG

Upstream 100 bases:

>100_bases
TGCTTATGGTTCACATTTTTATGTTATGATTATTTTTTCCATGAAGGAAATATGTTATGTTTATCAGACAATAAATTTTA
TTTATAGTTAAGAGGAATGC

Downstream 100 bases:

>100_bases
TTTATTTATATTTGTTCCTCAACAAATAACTCCCATAATTCTTCAGCATCTGGTGGCATTTTTTTTGCCGGTCGATAAAG
CCTTATTTCGATTGGTACCC

Product: transcriptional regulator LysR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MEIRQLEYFISASALGNLTKVAERHYVSQPNITVAIKKLESELGVNLFERRKNKLVLTEEGEFFLLKVKPLIIALKNSVA
EIKDYHNKNSGVVTLGIPPMISLFLFTPLFKHFRELYSEMELSLVEEGTFGLHQKLSHGDIDLAIVIINDAPEELVTVPL
MTQQHVVCISDKHPLAKKKTIDWEDLQNEPLIVMKKDSWHRKAILNECEKRGVNTHIFLSSNSVKNNIDLVAKNEGISFI
LDAVGLKCDGVTTRAMTEPVYVEIGLAWKKDKYLSYASRALIAFLEQYIKDNFQ

Sequences:

>Translated_294_residues
MEIRQLEYFISASALGNLTKVAERHYVSQPNITVAIKKLESELGVNLFERRKNKLVLTEEGEFFLLKVKPLIIALKNSVA
EIKDYHNKNSGVVTLGIPPMISLFLFTPLFKHFRELYSEMELSLVEEGTFGLHQKLSHGDIDLAIVIINDAPEELVTVPL
MTQQHVVCISDKHPLAKKKTIDWEDLQNEPLIVMKKDSWHRKAILNECEKRGVNTHIFLSSNSVKNNIDLVAKNEGISFI
LDAVGLKCDGVTTRAMTEPVYVEIGLAWKKDKYLSYASRALIAFLEQYIKDNFQ
>Mature_294_residues
MEIRQLEYFISASALGNLTKVAERHYVSQPNITVAIKKLESELGVNLFERRKNKLVLTEEGEFFLLKVKPLIIALKNSVA
EIKDYHNKNSGVVTLGIPPMISLFLFTPLFKHFRELYSEMELSLVEEGTFGLHQKLSHGDIDLAIVIINDAPEELVTVPL
MTQQHVVCISDKHPLAKKKTIDWEDLQNEPLIVMKKDSWHRKAILNECEKRGVNTHIFLSSNSVKNNIDLVAKNEGISFI
LDAVGLKCDGVTTRAMTEPVYVEIGLAWKKDKYLSYASRALIAFLEQYIKDNFQ

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=283, Percent_Identity=24.0282685512367, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1790399, Length=279, Percent_Identity=25.4480286738351, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI145693105, Length=295, Percent_Identity=26.4406779661017, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788296, Length=229, Percent_Identity=28.3842794759825, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=26.6211604095563, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI2367136, Length=298, Percent_Identity=21.4765100671141, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789204, Length=266, Percent_Identity=22.9323308270677, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788748, Length=282, Percent_Identity=23.0496453900709, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788887, Length=186, Percent_Identity=27.9569892473118, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787879, Length=195, Percent_Identity=24.6153846153846, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789506, Length=288, Percent_Identity=20.8333333333333, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787530, Length=227, Percent_Identity=22.4669603524229, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787601, Length=251, Percent_Identity=20.3187250996016, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788481, Length=202, Percent_Identity=23.2673267326733, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788297, Length=147, Percent_Identity=25.8503401360544, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789173, Length=140, Percent_Identity=28.5714285714286, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33440; Mature: 33440

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRQLEYFISASALGNLTKVAERHYVSQPNITVAIKKLESELGVNLFERRKNKLVLTEE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHEHHHHHHCCHHHHCCCCCEEEEEC
GEFFLLKVKPLIIALKNSVAEIKDYHNKNSGVVTLGIPPMISLFLFTPLFKHFRELYSEM
CCEEEEEECEEEEEHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
ELSLVEEGTFGLHQKLSHGDIDLAIVIINDAPEELVTVPLMTQQHVVCISDKHPLAKKKT
HHHHHCCCCCCHHHHCCCCCEEEEEEEECCCCHHHEEEEECCCCEEEEECCCCCCCCCCC
IDWEDLQNEPLIVMKKDSWHRKAILNECEKRGVNTHIFLSSNSVKNNIDLVAKNEGISFI
CCHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCEEE
LDAVGLKCDGVTTRAMTEPVYVEIGLAWKKDKYLSYASRALIAFLEQYIKDNFQ
EHHHCCEECCEEHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEIRQLEYFISASALGNLTKVAERHYVSQPNITVAIKKLESELGVNLFERRKNKLVLTEE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHEHHHHHHCCHHHHCCCCCEEEEEC
GEFFLLKVKPLIIALKNSVAEIKDYHNKNSGVVTLGIPPMISLFLFTPLFKHFRELYSEM
CCEEEEEECEEEEEHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
ELSLVEEGTFGLHQKLSHGDIDLAIVIINDAPEELVTVPLMTQQHVVCISDKHPLAKKKT
HHHHHCCCCCCHHHHCCCCCEEEEEEEECCCCHHHEEEEECCCCEEEEECCCCCCCCCCC
IDWEDLQNEPLIVMKKDSWHRKAILNECEKRGVNTHIFLSSNSVKNNIDLVAKNEGISFI
CCHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCEEE
LDAVGLKCDGVTTRAMTEPVYVEIGLAWKKDKYLSYASRALIAFLEQYIKDNFQ
EHHHCCEECCEEHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]