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The map label for this gene is ramA [H]

Identifier: 152969127

GI number: 152969127

Start: 623947

End: 624288

Strand: Direct

Name: ramA [H]

Synonym: KPN_00556

Alternate gene names: 152969127

Gene position: 623947-624288 (Clockwise)

Preceding gene: 152969117

Following gene: 152969130

Centisome position: 11.74

GC content: 55.26

Gene sequence:

>342_bases
ATGACGATTTCCGCTCAGGTGATTGATACTATCGTCGAGTGGATTGATGATAACCTGCATCAACCGCTGCGTATTGATGA
TATCGCTCGCCATGCCGGGTATTCGAAATGGCATCTGCAACGGCTGTTTTTACAGTACAAAGGGGAGAGCCTGGGGCGCT
ATATTCGCGAAAGGAAGCTGCTGCTGGCCGCCCGCGATCTGCGCGACACCGATCAGCGGGTCTACGATATCTGCCTCAAG
TATGGCTTCGATTCGCAACAGACCTTTACCCGCGTCTTCACCCGGACCTTCAATCAGCCGCCGGGCGCCTACCGCAAAGA
GAACCACAGTCGCGCCCACTGA

Upstream 100 bases:

>100_bases
GACGCCTGTGCAGGGCGAGCCGGTGTGGGTCGCCGATAAGACGCAATCATTTAACGCCTGGTGGCGCTAAGCGCCAGTGC
AGTATCAGAGGAGAGAGCAT

Downstream 100 bases:

>100_bases
GGCGCGCCTCTCCTGGTTTACCGCTCGAGGGAAAACCAGCGACGCCACACCCAGCGCAGCACCAGATACTCAATGGCGCC
CAGCGCTAAGAACCACAGAC

Product: putative regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 113; Mature: 112

Protein sequence:

>113_residues
MTISAQVIDTIVEWIDDNLHQPLRIDDIARHAGYSKWHLQRLFLQYKGESLGRYIRERKLLLAARDLRDTDQRVYDICLK
YGFDSQQTFTRVFTRTFNQPPGAYRKENHSRAH

Sequences:

>Translated_113_residues
MTISAQVIDTIVEWIDDNLHQPLRIDDIARHAGYSKWHLQRLFLQYKGESLGRYIRERKLLLAARDLRDTDQRVYDICLK
YGFDSQQTFTRVFTRTFNQPPGAYRKENHSRAH
>Mature_112_residues
TISAQVIDTIVEWIDDNLHQPLRIDDIARHAGYSKWHLQRLFLQYKGESLGRYIRERKLLLAARDLRDTDQRVYDICLKY
GFDSQQTFTRVFTRTFNQPPGAYRKENHSRAH

Specific function: Probable transcriptional activator [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790497, Length=100, Percent_Identity=49, Blast_Score=110, Evalue=2e-26,
Organism=Escherichia coli, GI87081928, Length=104, Percent_Identity=45.1923076923077, Blast_Score=109, Evalue=4e-26,
Organism=Escherichia coli, GI1790857, Length=102, Percent_Identity=49.0196078431373, Blast_Score=105, Evalue=8e-25,

Paralogues:

None

Copy number: 2500 (L-broth) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 13495; Mature: 13364

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISAQVIDTIVEWIDDNLHQPLRIDDIARHAGYSKWHLQRLFLQYKGESLGRYIRERKL
CCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
LLAARDLRDTDQRVYDICLKYGFDSQQTFTRVFTRTFNQPPGAYRKENHSRAH
HHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TISAQVIDTIVEWIDDNLHQPLRIDDIARHAGYSKWHLQRLFLQYKGESLGRYIRERKL
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
LLAARDLRDTDQRVYDICLKYGFDSQQTFTRVFTRTFNQPPGAYRKENHSRAH
HHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2193928; 7551053 [H]