Click here to switch to the map view.

The map label for this gene is hisJ [H]

Identifier: 152969095

GI number: 152969095

Start: 580639

End: 581382

Strand: Direct

Name: hisJ [H]

Synonym: KPN_00524

Alternate gene names: 152969095

Gene position: 580639-581382 (Clockwise)

Preceding gene: 152969094

Following gene: 152969096

Centisome position: 10.92

GC content: 56.99

Gene sequence:

>744_bases
ATGGCGTCGACGTCGGCGCTGGCCGAAGGGGGAAAGGAGATCCGCTTCGGCGTCGACCCGACTTTCGCGCCGTTTGAATG
GAAAGATCCGCAGGGCAAGCTGGCGGGGTTTGATATCGATTTAGGCAACGCCATTTGTCAGCAGCTGCAGGCGAAATGCG
TGTGGGTGGAAAGCAATTTCGACGGCATTATCCCGGCGCTGAAGGCGCGTAAATTCGACGCGATCCTTTCCGGGATGTAC
ATGACCGAGAAGCGCAAAGCGCAGATTGCCTTTAGCGACAAGTTGTATAACGGACCGGTGTTCCTCGTGGCGCGTAAAAA
TACCCTGCAAGGCAATACGCCGGAACAGCTGAAGGGCAAAACCATCGGCGTGGAGCAGGGCTCGGCGCAGGAGACGTATG
TTAACCAGCACTGGCGGCCGCAGGGGATAAACATTGTGGCTTACCAGGGGGCCGATAGCGTGGTGCGTGACCTGGAGTCG
GGGCGCATCGACGGCGCGGTGCTCTCCGGGATGATGGCCGACTACAGCTTCCTGCAGCAGCCGCAGGGTAAAGAATTCGC
CTTTGTCGGCGGCCATCTGCAGGACGATACGCTGTTTGGCGCCGGGGCGGCGATAGGTTTGCGTAAAGACGATGAGGCGC
TGCGTCAGGAAATTAACGGCGCGATTGCGAAGATTCTTGCCGACGGCACCTATAAAAAATTAGCTGGCAAATATTTTAGC
TTTGATGTTTATTCCGGGACATAA

Upstream 100 bases:

>100_bases
ATCGGCGCGACGCTGTTCGCCCGTCTGGTGGAAAAACACTGTCGCTAATCTCTGGAGAAGAACGCGAATGAAAAAATCAC
TATTGCTATGGCTGGCATTA

Downstream 100 bases:

>100_bases
TGTCAGAGTGTACCTGCGGGCGTGGAGCGCCCGCCGTAAGATTCCTTTTCTGATTATCCAATCGACCTCCTCTCCTGACC
CCTGTGGTTACCTGAGGGAA

Product: extracellular solute-binding family protein 3

Products: ADP; phosphate; L-histidine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MASTSALAEGGKEIRFGVDPTFAPFEWKDPQGKLAGFDIDLGNAICQQLQAKCVWVESNFDGIIPALKARKFDAILSGMY
MTEKRKAQIAFSDKLYNGPVFLVARKNTLQGNTPEQLKGKTIGVEQGSAQETYVNQHWRPQGINIVAYQGADSVVRDLES
GRIDGAVLSGMMADYSFLQQPQGKEFAFVGGHLQDDTLFGAGAAIGLRKDDEALRQEINGAIAKILADGTYKKLAGKYFS
FDVYSGT

Sequences:

>Translated_247_residues
MASTSALAEGGKEIRFGVDPTFAPFEWKDPQGKLAGFDIDLGNAICQQLQAKCVWVESNFDGIIPALKARKFDAILSGMY
MTEKRKAQIAFSDKLYNGPVFLVARKNTLQGNTPEQLKGKTIGVEQGSAQETYVNQHWRPQGINIVAYQGADSVVRDLES
GRIDGAVLSGMMADYSFLQQPQGKEFAFVGGHLQDDTLFGAGAAIGLRKDDEALRQEINGAIAKILADGTYKKLAGKYFS
FDVYSGT
>Mature_246_residues
ASTSALAEGGKEIRFGVDPTFAPFEWKDPQGKLAGFDIDLGNAICQQLQAKCVWVESNFDGIIPALKARKFDAILSGMYM
TEKRKAQIAFSDKLYNGPVFLVARKNTLQGNTPEQLKGKTIGVEQGSAQETYVNQHWRPQGINIVAYQGADSVVRDLESG
RIDGAVLSGMMADYSFLQQPQGKEFAFVGGHLQDDTLFGAGAAIGLRKDDEALRQEINGAIAKILADGTYKKLAGKYFSF
DVYSGT

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788648, Length=235, Percent_Identity=51.9148936170213, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1788649, Length=243, Percent_Identity=48.9711934156379, Blast_Score=246, Evalue=7e-67,
Organism=Escherichia coli, GI1787085, Length=239, Percent_Identity=36.4016736401674, Blast_Score=164, Evalue=4e-42,
Organism=Escherichia coli, GI1787088, Length=245, Percent_Identity=31.8367346938775, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1787031, Length=229, Percent_Identity=29.6943231441048, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788228, Length=235, Percent_Identity=28.0851063829787, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786876, Length=244, Percent_Identity=27.4590163934426, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: 3400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26875; Mature: 26744

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASTSALAEGGKEIRFGVDPTFAPFEWKDPQGKLAGFDIDLGNAICQQLQAKCVWVESNF
CCCCHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHCCEEEEEECCC
DGIIPALKARKFDAILSGMYMTEKRKAQIAFSDKLYNGPVFLVARKNTLQGNTPEQLKGK
CCCHHHHHHHHHHHHHHCHHHCCCCCEEEEEECEECCCCEEEEEECCCCCCCCHHHHCCC
TIGVEQGSAQETYVNQHWRPQGINIVAYQGADSVVRDLESGRIDGAVLSGMMADYSFLQQ
EEECCCCCCCHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
PQGKEFAFVGGHLQDDTLFGAGAAIGLRKDDEALRQEINGAIAKILADGTYKKLAGKYFS
CCCCEEEEECCCCCCCCEECCCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCEEE
FDVYSGT
EEEECCC
>Mature Secondary Structure 
ASTSALAEGGKEIRFGVDPTFAPFEWKDPQGKLAGFDIDLGNAICQQLQAKCVWVESNF
CCCHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHCCEEEEEECCC
DGIIPALKARKFDAILSGMYMTEKRKAQIAFSDKLYNGPVFLVARKNTLQGNTPEQLKGK
CCCHHHHHHHHHHHHHHCHHHCCCCCEEEEEECEECCCCEEEEEECCCCCCCCHHHHCCC
TIGVEQGSAQETYVNQHWRPQGINIVAYQGADSVVRDLESGRIDGAVLSGMMADYSFLQQ
EEECCCCCCCHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
PQGKEFAFVGGHLQDDTLFGAGAAIGLRKDDEALRQEINGAIAKILADGTYKKLAGKYFS
CCCCEEEEECCCCCCCCEECCCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCEEE
FDVYSGT
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]