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The map label for this gene is cueO [H]

Identifier: 152968712

GI number: 152968712

Start: 155627

End: 157225

Strand: Direct

Name: cueO [H]

Synonym: KPN_00131

Alternate gene names: 152968712

Gene position: 155627-157225 (Clockwise)

Preceding gene: 152968706

Following gene: 152968714

Centisome position: 2.93

GC content: 61.85

Gene sequence:

>1599_bases
ATGCAACGTCGAGACTTCCTCAAACTGACCGCCGCGGTCGGCATGGCCAGCGCATTGCCGTTATGGAGCCGGGCGGTCTT
TGCCGCCAGTCGCCCGGCTCTGCCGATACCGTCGCTGTTAGCCGCCGATGCGCGCAACCGCATCGCGCTCAGGATCCAGG
CGGGCAAAACGCGGTTTGGCGCCCTGAATGCCACTACCTGGGGCTACAACGGGTCGCTGCTGGGCCCTGCGCTGCAGTTG
ACGCAGGGGAAGACCGTCACGGTGGATATTACTAACCAGCTGGCGGAAGAGACCACGCTGCACTGGCATGGTCTGGAGGT
ACCGGGCGAGGTCGACGGGGGGCCACAGGGGGTTATCGCGCCGGGTGCCACCCGCACGGTAAGCTTCACGCCAACGCAGC
GGGCGGCGACCTGCTGGTTTCATCCGCATCAGCATGGGAGCACCGGCCGGCAGGTGGCGATGGGGCTGGCCGGCCTGGTG
CTGATCGAGGATGAAGAGAGCGGGCGCCTGCTGCTGCCTAAGCAGTGGGGAATCGATGATGTGCCGGTGATCGTCCAGGA
TAAGAAGTTCACCGCCGCCGGCGAGATCGACTATCAGCTGGATGTGATGAGCGCCGCCGTCGGCTGGTTTGGCGATACGC
TACTGACCAATGGGGCCCTCTATCCCGAACATGCCGCGCCGCGCGGCTGGCTGCGCCTGCGCTTGCTGAACGGCTGTAAT
GCCCGTTCCCTCAATTTTGCCACCAGCGATAAGCGCCCGCTGTACGTGGTGGCCAGCGATGGCGGTCTGTTGGCCGAGCC
GGTCAAGGTCGATGAATTGCCGGTCTTGATGGGTGAACGCTTTGAGGTGCTGGTGGACACCAGCGACGGTAAACCTTTCG
ATCTGGTGACCCTGCCGGTGAGCCAGATGGGCATGGCGATTGCGCCTTTTGATAAACCACAGCCGGTACTGCGCGTCCAG
CCGCTGGTCATTCCCGCCTCCGGCAAGCTGCTGGATACTCTGGCCGCTCTCCCGGCGTTACCGTCCCTGACGGGGCTGAC
GCAGCGTCAGCTCCAGCTGTCGATGGATCCGATGCTCGACCGGATGGGCATGCAGGCGCTGATGGAGAAGTATGGCGACC
AGGCGATGGCCGGAATGGATCACGGCATGATGGGGCATGGCGACATGAGCGACATGGGGAATATGCATCACGGCGACATG
AGCATGAACCACGGATCCGGCATGGAGCATGGCATGTCGTCAGGCAAGGGCTTTGATTTCCATAACGCCAACCGCATCAA
CGGCAAAGCCTTCGACATGAATGAGCCGATGTTTGCCGCCGCCAGAGGCCAGTATGAGCGCTGGGTGATTTCAGGTAAGG
GCGACATGATGTTGCATCCGTTCCATATCCACGGCACCCAGTTCCGTATCCTCAGCGAAAACGGCAAGCCGCCAGCGGCG
CATCGTCGCGGCTGGAAAGACACCGTTCGTGTGGAAGGGGACGTGAGTGAAGTATTAGTGAAGTTTGATCATCCGGCGCC
GAAGGAGTTTGCCTATATGGCCCACTGCCACCTGCTGGAACATGAAGACACCGGGATGATGTTGGGGTTCACGGTTTAA

Upstream 100 bases:

>100_bases
TTACACAGCGCCGATCTTTATTCTTACTCTTCTGCTTGACCTTCCCGTTACGGTAGGGTTTAAGCTCAAATATTCGCCCG
CAGAATAAAAGGATGTGAAT

Downstream 100 bases:

>100_bases
GTCTCTGCGACCTGCTGCCATCCCCTACGGGAAGAAGGACGGAACACCAAAAAAAACGGCAACTTTCGTTGCCGTTTTGC
GTTTTATTTGGCGTCATCCG

Product: multicopper oxidase

Products: NA

Alternate protein names: Copper efflux oxidase [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MQRRDFLKLTAAVGMASALPLWSRAVFAASRPALPIPSLLAADARNRIALRIQAGKTRFGALNATTWGYNGSLLGPALQL
TQGKTVTVDITNQLAEETTLHWHGLEVPGEVDGGPQGVIAPGATRTVSFTPTQRAATCWFHPHQHGSTGRQVAMGLAGLV
LIEDEESGRLLLPKQWGIDDVPVIVQDKKFTAAGEIDYQLDVMSAAVGWFGDTLLTNGALYPEHAAPRGWLRLRLLNGCN
ARSLNFATSDKRPLYVVASDGGLLAEPVKVDELPVLMGERFEVLVDTSDGKPFDLVTLPVSQMGMAIAPFDKPQPVLRVQ
PLVIPASGKLLDTLAALPALPSLTGLTQRQLQLSMDPMLDRMGMQALMEKYGDQAMAGMDHGMMGHGDMSDMGNMHHGDM
SMNHGSGMEHGMSSGKGFDFHNANRINGKAFDMNEPMFAAARGQYERWVISGKGDMMLHPFHIHGTQFRILSENGKPPAA
HRRGWKDTVRVEGDVSEVLVKFDHPAPKEFAYMAHCHLLEHEDTGMMLGFTV

Sequences:

>Translated_532_residues
MQRRDFLKLTAAVGMASALPLWSRAVFAASRPALPIPSLLAADARNRIALRIQAGKTRFGALNATTWGYNGSLLGPALQL
TQGKTVTVDITNQLAEETTLHWHGLEVPGEVDGGPQGVIAPGATRTVSFTPTQRAATCWFHPHQHGSTGRQVAMGLAGLV
LIEDEESGRLLLPKQWGIDDVPVIVQDKKFTAAGEIDYQLDVMSAAVGWFGDTLLTNGALYPEHAAPRGWLRLRLLNGCN
ARSLNFATSDKRPLYVVASDGGLLAEPVKVDELPVLMGERFEVLVDTSDGKPFDLVTLPVSQMGMAIAPFDKPQPVLRVQ
PLVIPASGKLLDTLAALPALPSLTGLTQRQLQLSMDPMLDRMGMQALMEKYGDQAMAGMDHGMMGHGDMSDMGNMHHGDM
SMNHGSGMEHGMSSGKGFDFHNANRINGKAFDMNEPMFAAARGQYERWVISGKGDMMLHPFHIHGTQFRILSENGKPPAA
HRRGWKDTVRVEGDVSEVLVKFDHPAPKEFAYMAHCHLLEHEDTGMMLGFTV
>Mature_532_residues
MQRRDFLKLTAAVGMASALPLWSRAVFAASRPALPIPSLLAADARNRIALRIQAGKTRFGALNATTWGYNGSLLGPALQL
TQGKTVTVDITNQLAEETTLHWHGLEVPGEVDGGPQGVIAPGATRTVSFTPTQRAATCWFHPHQHGSTGRQVAMGLAGLV
LIEDEESGRLLLPKQWGIDDVPVIVQDKKFTAAGEIDYQLDVMSAAVGWFGDTLLTNGALYPEHAAPRGWLRLRLLNGCN
ARSLNFATSDKRPLYVVASDGGLLAEPVKVDELPVLMGERFEVLVDTSDGKPFDLVTLPVSQMGMAIAPFDKPQPVLRVQ
PLVIPASGKLLDTLAALPALPSLTGLTQRQLQLSMDPMLDRMGMQALMEKYGDQAMAGMDHGMMGHGDMSDMGNMHHGDM
SMNHGSGMEHGMSSGKGFDFHNANRINGKAFDMNEPMFAAARGQYERWVISGKGDMMLHPFHIHGTQFRILSENGKPPAA
HRRGWKDTVRVEGDVSEVLVKFDHPAPKEFAYMAHCHLLEHEDTGMMLGFTV

Specific function: Probably involved in periplasmic detoxification of copper by oxidizing Cu(+) to Cu(2+) and thus preventing its uptake into the cytoplasm. Possesses phenoloxidase and ferroxidase activities and might be involved in the production of polyphenolic compounds

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 plastocyanin-like domains [H]

Homologues:

Organism=Escherichia coli, GI1786314, Length=533, Percent_Identity=79.3621013133208, Blast_Score=867, Evalue=0.0,
Organism=Escherichia coli, GI1789394, Length=528, Percent_Identity=32.3863636363636, Blast_Score=242, Evalue=5e-65,
Organism=Drosophila melanogaster, GI24650186, Length=235, Percent_Identity=26.8085106382979, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011706
- InterPro:   IPR011707
- InterPro:   IPR002355
- InterPro:   IPR008972
- InterPro:   IPR006311 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07731 Cu-oxidase_2; PF07732 Cu-oxidase_3 [H]

EC number: NA

Molecular weight: Translated: 57862; Mature: 57862

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00080 MULTICOPPER_OXIDASE2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRRDFLKLTAAVGMASALPLWSRAVFAASRPALPIPSLLAADARNRIALRIQAGKTRFG
CCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHCCCCEEEEEEEECCCEEC
ALNATTWGYNGSLLGPALQLTQGKTVTVDITNQLAEETTLHWHGLEVPGEVDGGPQGVIA
EEECEECCCCCCCCCCEEEECCCCEEEEEEHHHHHHHCEEEEECCCCCCCCCCCCCCEEC
PGATRTVSFTPTQRAATCWFHPHQHGSTGRQVAMGLAGLVLIEDEESGRLLLPKQWGIDD
CCCCEEEEECCCCCCEEEEECCCCCCCCHHHHHHCCEEEEEEEECCCCCEECCCCCCCCC
VPVIVQDKKFTAAGEIDYQLDVMSAAVGWFGDTLLTNGALYPEHAAPRGWLRLRLLNGCN
CCEEEECCCEEECCCCEEEEEHHHHHHHHHCHHHHHCCCCCCCCCCCCCEEEEEEECCCC
ARSLNFATSDKRPLYVVASDGGLLAEPVKVDELPVLMGERFEVLVDTSDGKPFDLVTLPV
CCEEEEECCCCCEEEEEECCCCEEECCCCCCCCCEECCCCEEEEEECCCCCEEEEEEECH
SQMGMAIAPFDKPQPVLRVQPLVIPASGKLLDTLAALPALPSLTGLTQRQLQLSMDPMLD
HHCCEEEECCCCCCCEEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHH
RMGMQALMEKYGDQAMAGMDHGMMGHGDMSDMGNMHHGDMSMNHGSGMEHGMSSGKGFDF
HHHHHHHHHHHCCHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCC
HNANRINGKAFDMNEPMFAAARGQYERWVISGKGDMMLHPFHIHGTQFRILSENGKPPAA
CCCCCCCCEEECCCCCEEEECCCCCEEEEEECCCCEEEEEEEECCEEEEEEECCCCCCHH
HRRGWKDTVRVEGDVSEVLVKFDHPAPKEFAYMAHCHLLEHEDTGMMLGFTV
HHCCCCCEEEECCCHHHEEEECCCCCCHHHHHEEEEEEEEECCCCEEEEEEC
>Mature Secondary Structure
MQRRDFLKLTAAVGMASALPLWSRAVFAASRPALPIPSLLAADARNRIALRIQAGKTRFG
CCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHCCCCEEEEEEEECCCEEC
ALNATTWGYNGSLLGPALQLTQGKTVTVDITNQLAEETTLHWHGLEVPGEVDGGPQGVIA
EEECEECCCCCCCCCCEEEECCCCEEEEEEHHHHHHHCEEEEECCCCCCCCCCCCCCEEC
PGATRTVSFTPTQRAATCWFHPHQHGSTGRQVAMGLAGLVLIEDEESGRLLLPKQWGIDD
CCCCEEEEECCCCCCEEEEECCCCCCCCHHHHHHCCEEEEEEEECCCCCEECCCCCCCCC
VPVIVQDKKFTAAGEIDYQLDVMSAAVGWFGDTLLTNGALYPEHAAPRGWLRLRLLNGCN
CCEEEECCCEEECCCCEEEEEHHHHHHHHHCHHHHHCCCCCCCCCCCCCEEEEEEECCCC
ARSLNFATSDKRPLYVVASDGGLLAEPVKVDELPVLMGERFEVLVDTSDGKPFDLVTLPV
CCEEEEECCCCCEEEEEECCCCEEECCCCCCCCCEECCCCEEEEEECCCCCEEEEEEECH
SQMGMAIAPFDKPQPVLRVQPLVIPASGKLLDTLAALPALPSLTGLTQRQLQLSMDPMLD
HHCCEEEECCCCCCCEEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHH
RMGMQALMEKYGDQAMAGMDHGMMGHGDMSDMGNMHHGDMSMNHGSGMEHGMSSGKGFDF
HHHHHHHHHHHCCHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCC
HNANRINGKAFDMNEPMFAAARGQYERWVISGKGDMMLHPFHIHGTQFRILSENGKPPAA
CCCCCCCCEEECCCCCEEEECCCCCEEEEEECCCCEEEEEEEECCEEEEEEECCCCCCHH
HRRGWKDTVRVEGDVSEVLVKFDHPAPKEFAYMAHCHLLEHEDTGMMLGFTV
HHCCCCCEEEECCCHHHEEEECCCCCCHHHHHEEEEEEEEECCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]