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The map label for this gene is proP [H]

Identifier: 152968592

GI number: 152968592

Start: 10023

End: 11327

Strand: Direct

Name: proP [H]

Synonym: KPN_00010

Alternate gene names: 152968592

Gene position: 10023-11327 (Clockwise)

Preceding gene: 152968591

Following gene: 152968596

Centisome position: 0.19

GC content: 62.15

Gene sequence:

>1305_bases
ATGTCATATGCAAACCGACCGCTCAACCGCCAGGATTATAAAACGCTGACGCTGGCCGCCCTGGGCGGCGCGCTGGAGTT
TTATGACTTTATCATTTTCGTTTTCTTCGCCGCCGTCGTCGGCGAGCTCTTCTTCCCTGCGGATATTCCCGAATGGCTGC
GCCAGGTGCAAACTTTTGGCATTTTCGCCGCCGGTTACCTGGCGCGTCCGCTGGGGGGCATCATCATGGCCCACTTTGGC
GATCTGGTGGGGCGCAAGAAAATGTTCACCCTCAGCATCCTGCTGATGGCGCTGCCAACCCTGGCGATCGGTCTGCTACC
GACCTATAGCTCGGTAGGTATCATCGCCCCGCTGCTATTGCTGCTGATGCGTATTCTGCAGGGAGCGGCGATCGGCGGTG
AAGTTCCAGGCGCCTGGGTGTTTGTCGCTGAGCATGTCCCGGAAAAACGCATTGGTATCGCCTGCGGCACGCTGACGGCC
GGCCTGACGGTTGGCATTCTGCTGGGGTCGGTGGTGGCCACGCTTATCAATACCAGTCTGACGCCGCAGGGGATCCACGA
CGGCGGCTGGCGGATCCCCTTCCTGCTGGGCGGCGTATTTGGTCTGATAGCGATGTATCTGCGCCGCTGGCTGCAGGAGA
CGCCGATTTTTCTTGAGATGCAGCAGCGCAAAGCGCTGGCGCAGGAGCTGCCGGTAAAAGCGGTGGCGCTCAAGCACCAG
AAGGCGGTGGTGATCTCTATGCTGCTGACCTGGCTGCTGTCGGCTGGCGTGGTGGTGGTGATCCTGATGTCGCCGGTCTG
GCTGCAGAAGCACTATGGTTTTGCGCCGGCCATTACCCTGCAGGCCAACAGCATCGCCACCATTATGTTGTGCATCGGCT
GTCTGCTCGCCGGGCTGGCGGCGGACCGCTTCGGCGCCAGCCGCACTTTTATCGTCGGCAGCCTGCTGCTGGCGGTTGCC
AGCTGGGCATTTTACCATCTTGCCGGCGCCAGCCCGCAGCGGCTGTTCCTGCTCTACGGTACGGTGGGGCTTTGCGTCGG
CGTGGTGGGGGCGGTGCCCTATGTGATGGTGCGGGCCTTCCCGGCGGAAGTACGCTTCACCGGCATCTCTTTCTCCTACA
ACCTGTCGTACGCGATCTTTGGCGGCCTGACGCCGATTGCGGTCACCATGCTGATGGGGGTGTCGCCGATGGCGCCGGCC
TGGTATGTGCTGGCCCTGTCGCTGATGGGGCTAGGATTAGGTATCTGGCTGCGTCAGGAGCTGACGGCGCCGACGGGGAT
GCCGGAAGGGGAGCTGCAGCGTTAA

Upstream 100 bases:

>100_bases
TAACCTGCATCGCTAATGGTGTGCCGTTTTATTTACGATATAGTGATTTTTACTTTACACACTATAAAACATGCTGCACA
CCTCAGGATGAAATTGCGTT

Downstream 100 bases:

>100_bases
CGATGGCTAGCTGATGCTGATAATGACCTGCCGCCGCCCTGTGACCGGACGGCGGCGGAAGATTAGCGCGGTTCGCCGAT
CGGCAGCACGGTGCGGCCGA

Product: low-affinity transport system; proline permease II

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MSYANRPLNRQDYKTLTLAALGGALEFYDFIIFVFFAAVVGELFFPADIPEWLRQVQTFGIFAAGYLARPLGGIIMAHFG
DLVGRKKMFTLSILLMALPTLAIGLLPTYSSVGIIAPLLLLLMRILQGAAIGGEVPGAWVFVAEHVPEKRIGIACGTLTA
GLTVGILLGSVVATLINTSLTPQGIHDGGWRIPFLLGGVFGLIAMYLRRWLQETPIFLEMQQRKALAQELPVKAVALKHQ
KAVVISMLLTWLLSAGVVVVILMSPVWLQKHYGFAPAITLQANSIATIMLCIGCLLAGLAADRFGASRTFIVGSLLLAVA
SWAFYHLAGASPQRLFLLYGTVGLCVGVVGAVPYVMVRAFPAEVRFTGISFSYNLSYAIFGGLTPIAVTMLMGVSPMAPA
WYVLALSLMGLGLGIWLRQELTAPTGMPEGELQR

Sequences:

>Translated_434_residues
MSYANRPLNRQDYKTLTLAALGGALEFYDFIIFVFFAAVVGELFFPADIPEWLRQVQTFGIFAAGYLARPLGGIIMAHFG
DLVGRKKMFTLSILLMALPTLAIGLLPTYSSVGIIAPLLLLLMRILQGAAIGGEVPGAWVFVAEHVPEKRIGIACGTLTA
GLTVGILLGSVVATLINTSLTPQGIHDGGWRIPFLLGGVFGLIAMYLRRWLQETPIFLEMQQRKALAQELPVKAVALKHQ
KAVVISMLLTWLLSAGVVVVILMSPVWLQKHYGFAPAITLQANSIATIMLCIGCLLAGLAADRFGASRTFIVGSLLLAVA
SWAFYHLAGASPQRLFLLYGTVGLCVGVVGAVPYVMVRAFPAEVRFTGISFSYNLSYAIFGGLTPIAVTMLMGVSPMAPA
WYVLALSLMGLGLGIWLRQELTAPTGMPEGELQR
>Mature_433_residues
SYANRPLNRQDYKTLTLAALGGALEFYDFIIFVFFAAVVGELFFPADIPEWLRQVQTFGIFAAGYLARPLGGIIMAHFGD
LVGRKKMFTLSILLMALPTLAIGLLPTYSSVGIIAPLLLLLMRILQGAAIGGEVPGAWVFVAEHVPEKRIGIACGTLTAG
LTVGILLGSVVATLINTSLTPQGIHDGGWRIPFLLGGVFGLIAMYLRRWLQETPIFLEMQQRKALAQELPVKAVALKHQK
AVVISMLLTWLLSAGVVVVILMSPVWLQKHYGFAPAITLQANSIATIMLCIGCLLAGLAADRFGASRTFIVGSLLLAVAS
WAFYHLAGASPQRLFLLYGTVGLCVGVVGAVPYVMVRAFPAEVRFTGISFSYNLSYAIFGGLTPIAVTMLMGVSPMAPAW
YVLALSLMGLGLGIWLRQELTAPTGMPEGELQR

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=429, Percent_Identity=30.5361305361305, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI1788292, Length=418, Percent_Identity=30.1435406698565, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1789941, Length=381, Percent_Identity=32.2834645669291, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1788942, Length=433, Percent_Identity=28.4064665127021, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI87082231, Length=434, Percent_Identity=22.8110599078341, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082404, Length=408, Percent_Identity=22.0588235294118, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46673; Mature: 46541

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCCCCCCCCC
>Mature Secondary Structure 
SYANRPLNRQDYKTLTLAALGGALEFYDFIIFVFFAAVVGELFFPADIPEWLRQVQTFG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
IFAAGYLARPLGGIIMAHFGDLVGRKKMFTLSILLMALPTLAIGLLPTYSSVGIIAPLLL
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LLMRILQGAAIGGEVPGAWVFVAEHVPEKRIGIACGTLTAGLTVGILLGSVVATLINTSL
HHHHHHCCCCCCCCCCCCEEEEEHHCCCHHHCEEHHHHHHHHHHHHHHHHHHHHHHHCCC
TPQGIHDGGWRIPFLLGGVFGLIAMYLRRWLQETPIFLEMQQRKALAQELPVKAVALKHQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHCCHHHHHHHHH
KAVVISMLLTWLLSAGVVVVILMSPVWLQKHYGFAPAITLQANSIATIMLCIGCLLAGLA
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHH
ADRFGASRTFIVGSLLLAVASWAFYHLAGASPQRLFLLYGTVGLCVGVVGAVPYVMVRAF
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHC
PAEVRFTGISFSYNLSYAIFGGLTPIAVTMLMGVSPMAPAWYVLALSLMGLGLGIWLRQE
CCCEEEEEEEEEECCCHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LTAPTGMPEGELQR
HCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]