Click here to switch to the map view.

The map label for this gene is hisH

Identifier: 151222785

GI number: 151222785

Start: 2841789

End: 2842367

Strand: Reverse

Name: hisH

Synonym: NWMN_2573

Alternate gene names: 151222785

Gene position: 2842367-2841789 (Counterclockwise)

Preceding gene: 151222786

Following gene: 151222784

Centisome position: 98.73

GC content: 36.44

Gene sequence:

>579_bases
ATGATTGTCATCGTTGATTATGGATTAGGGAATATTAGTAATGTAAAACGCGCTATTGAACATTTAGGGTATGAGGTGGT
TGTCTCAAATACCTCAAAAATAATCGATCAAGCAGAAACAATCATATTGCCCGGTGTCGGCCATTTTAAAGATGCGATGT
CAGAGATAAAACGATTAAATCTCAATGCAATATTGGCTAAGAATACTGATAAGAAGATGATTGGTATTTGTTTAGGCATG
CAATTAATGTATGAGCATAGTGATGAAGGCGATGCATCTGGATTAGGGTTTATCCCAGGAAATATTTCGCGTATCCAAAC
AGAATACCCAGTGCCACACTTAGGCTGGAATAATTTAGTGAGTAAGCACCCTATGTTAAATCAAGATGTTTATTTCGTAC
ATTCTTATCAAGCGCCGATGTCAGAAAATGTAATTGCATATGCGCAGTATGGGGCTGATATTCCGGCAATTGTTCAATTT
AACAATTATATTGGTATTCAATTCCATCCTGAAAAAAGCGGTACATATGGGTTACAAATTTTGCGTCAGGCAATACAAGG
GGGATTTATAAATGATTGA

Upstream 100 bases:

>100_bases
ATGAAATTGAAGCTATATTCAAAGCGTTTTCCCGTGCATTAGGCATAGCGCTAACTGCAACTGATGATCAGCGTGTGCCG
TCATCGAAAGGTGTGATTGA

Downstream 100 bases:

>100_bases
ATTATGGCCAGCGATTGATTTGATTGGGTCAACAAGTGTGAGGTTAACAGAGGGTAAATATGATAGTGAAGAAAAAATGT
CACGCTCGGCTGAAGAAAGT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILPGVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGM
QLMYEHSDEGDASGLGFIPGNISRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND

Sequences:

>Translated_192_residues
MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILPGVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGM
QLMYEHSDEGDASGLGFIPGNISRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND
>Mature_192_residues
MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILPGVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGM
QLMYEHSDEGDASGLGFIPGNISRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=195, Percent_Identity=36.4102564102564, Blast_Score=124, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=208, Percent_Identity=32.2115384615385, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 21280; Mature: 21280

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILPGVGHFKDAMSEIKRLN
CEEEEECCCCCHHHHHHHHHHCCEEEEECCHHHHHHCCCEEEECCCCHHHHHHHHHHHCC
LNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNISRIQTEYPVPHLGWNNLV
CEEEEECCCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHEEEECCCCCCCCHHHHH
SKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQFNNYIGIQFHPEKSGTYGLQI
HCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCEEEEECCEEEEEEECCCCCCHHHHH
LRQAIQGGFIND
HHHHHCCCCCCC
>Mature Secondary Structure
MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILPGVGHFKDAMSEIKRLN
CEEEEECCCCCHHHHHHHHHHCCEEEEECCHHHHHHCCCEEEECCCCHHHHHHHHHHHCC
LNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNISRIQTEYPVPHLGWNNLV
CEEEEECCCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCHHEEEECCCCCCCCHHHHH
SKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQFNNYIGIQFHPEKSGTYGLQI
HCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCEEEEECCEEEEEEECCCCCCHHHHH
LRQAIQGGFIND
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA