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The map label for this gene is ycnB [H]

Identifier: 151222526

GI number: 151222526

Start: 2540713

End: 2542071

Strand: Reverse

Name: ycnB [H]

Synonym: NWMN_2314

Alternate gene names: 151222526

Gene position: 2542071-2540713 (Counterclockwise)

Preceding gene: 151222527

Following gene: 151222525

Centisome position: 88.3

GC content: 34.44

Gene sequence:

>1359_bases
GTGACGGCTTACCCAAGTATTATGAAAGATTTTGAAATTTCATATACACAAGTTCAATGGCTGACAACAGCTTTTTTATT
GACTAATGGGATTGTTATTCCTTTGTCCGCGCTTGTTATACAACGTTATACAACAAGACAAGTGTTTTTAGTGGGTATTT
CTATCTTTTTCTTAGGTACATTACTCGGCGGCTTGAGTCCGCACTTTGCAACATTATTAGTTGCTAGAATTATTCAGGCG
TTAGGCGCAGGTATTATGATGCCATTGATGATGACAACGATTTTGGATGTTTTCCAACCACATGAACGCGGTAAATATAT
GGGGATATTTGGTTTGGTAATTGGTTTAGCACCAGCTATTGGACCTACTCTTTCAGGTTACCTTGTTGAATATTTTAACT
GGAGATCGCTTTTCCATGTTGTCGCTCCAATTGCAGCTGTGACATTTTTAATTGGATTTAAAACGATAAAAAATGTTGGA
ACTACAATTAAAGTACCTATTGATTTTATTTCTGTCATTTTTTCTGTACTAGGTTTCGGCGGGTTATTGTATGGAACGAG
TTCAATTTCAGAAAAAGGTTTTGATAATCCTATCGTATTAGTATCTATGATTGGAGGCGTTGTTTTAGTCGCATTATTTG
TATTACGTCAATATCGGCTATCAACACCATTATTAAATTTTGCTGTATTTAAAAATAAACAATTTACAGTTGGTATCATT
ATTATGGGTGTCACAATGGTATCGATGATTGGTTCGGAAACGATTTTACCTATCTTTGTGCAAAATTTATTGCATCGTTC
AGCTTTAGATTCTGGATTAACTTTATTACCAGGAGCAATTGTTATGGCATTTATGTCGATGACTTCGGGTGCTTTATATG
AAAAGTTTGGTCCTAGAAATCTTGCTTTAGTAGGTATGGCGATTGTTGTTATTACTACGGCTTATTTTGTTGTAATGGAT
GAACAAACATCAACAATCATGTTGGCAACAGTTTATGCGATTCGAATGGTTGGTATCGCGTTAGGATTAATTCCAGTAAT
GACCCATACGATGAATCAATTAAAGCCAGAAATGAATGCACATGGTTCATCTATGACAAACACAGTACAACAAATTGCTG
GTTCTATCGGAACAGCTGCATTAATTACTATTTTATCCCATGCAAGCAAAAACTTTTCTCCAACTATGTCAGACTATAAT
GGCATGAACAAAATTGATATGATGAATCAAATTAAAGTTGATACGATGTTGCATGGTTATCACGCTGGATTTTTATTTGC
ATTATTGATTACAGTTGTTAGTTTCTTTTGTTCATTTATGTTACAAGGTAAGAAAAAAGAAGTTGATTCTCGTCAATAA

Upstream 100 bases:

>100_bases
GTGAGGGGGGAAGCTTTAAATGTCGAAGAAACAAAAATTAACGATGATTATTACTATGCTGATGGGTGGATTTTTGGATT
ATTAATGAAAAACACTATTA

Downstream 100 bases:

>100_bases
ATATAAGTTGCTAGCTATATAAAGCTTTTTAGCAAAAGTTCAACATATTGACTTATCCGGCATTTCAGATTAAAATATTT
TTATTCCGATTAGAATAATA

Product: drug resistance transporter EmrB/QacA subfamily protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MTAYPSIMKDFEISYTQVQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVARIIQA
LGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFNWRSLFHVVAPIAAVTFLIGFKTIKNVG
TTIKVPIDFISVIFSVLGFGGLLYGTSSISEKGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGII
IMGVTMVSMIGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMAIVVITTAYFVVMD
EQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSMTNTVQQIAGSIGTAALITILSHASKNFSPTMSDYN
GMNKIDMMNQIKVDTMLHGYHAGFLFALLITVVSFFCSFMLQGKKKEVDSRQ

Sequences:

>Translated_452_residues
MTAYPSIMKDFEISYTQVQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVARIIQA
LGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFNWRSLFHVVAPIAAVTFLIGFKTIKNVG
TTIKVPIDFISVIFSVLGFGGLLYGTSSISEKGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGII
IMGVTMVSMIGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMAIVVITTAYFVVMD
EQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSMTNTVQQIAGSIGTAALITILSHASKNFSPTMSDYN
GMNKIDMMNQIKVDTMLHGYHAGFLFALLITVVSFFCSFMLQGKKKEVDSRQ
>Mature_451_residues
TAYPSIMKDFEISYTQVQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVARIIQAL
GAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFNWRSLFHVVAPIAAVTFLIGFKTIKNVGT
TIKVPIDFISVIFSVLGFGGLLYGTSSISEKGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIII
MGVTMVSMIGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMAIVVITTAYFVVMDE
QTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSMTNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNG
MNKIDMMNQIKVDTMLHGYHAGFLFALLITVVSFFCSFMLQGKKKEVDSRQ

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=434, Percent_Identity=24.4239631336406, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1788710, Length=395, Percent_Identity=24.8101265822785, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790195, Length=388, Percent_Identity=25, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI87081983, Length=394, Percent_Identity=23.6040609137056, Blast_Score=102, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6323735, Length=299, Percent_Identity=27.0903010033445, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=151, Percent_Identity=24.5033112582781, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49230; Mature: 49098

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TAYPSIMKDFEISYTQVQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGT
CCCHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]