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The map label for this gene is gltT [H]

Identifier: 151222496

GI number: 151222496

Start: 2509881

End: 2511158

Strand: Reverse

Name: gltT [H]

Synonym: NWMN_2284

Alternate gene names: 151222496

Gene position: 2511158-2509881 (Counterclockwise)

Preceding gene: 151222497

Following gene: 151222491

Centisome position: 87.23

GC content: 32.86

Gene sequence:

>1278_bases
ATGGCTCTATTCAAGAGAAAAATTAGCCTACCTATGCAAGTTGTTATTGCATTAGTGTTAGGTGTTGTCGTAGGACTTTT
ATTATATGGACAAGAAAACGTAGCAAATTACATTAAACCATTTGGTGACGTATTTTTAAATTTAATTAAAATGATCGTTA
TACCAGTTGTATTTTGCTCACTAGCGCTTTCTATTTCGAACGTTGGGGAATCGAAAACTGTAGGGCGTTATGGCTGGAAA
ACAATTTTATACTTTGAAATTATTACAACAATCGCAATAGGTTTAGGGATTATCTTCGGTAACCTATTTAAACCAGGTGC
TGGATTAGACCCAACAAAATTACCTAAAGGTGATATTTCTAAATATCAATCAACTGCACATGCAGCAGAACAATCTACAT
ATGGAAATCATTTTATTGATACCATTGTACATATTATTCCGACAAACTTTTTTGAAGCTTTAAATAAGGGTGAATTATTA
CCTATTATCTTCTTCGCAGTATTCTTTGGATTAGGATTAGCTGCTGTAGGTAAAAAAGCAGAACCAGTTAAAGAATTTTT
AAGCGGATCGCTTGAAGCTGTGTTCTGGATGATTAATAAAATTTTAAAATTAGCACCACTTGGAGTGTTTGCATTCATTT
GTACTACAATTATTACATTTGGTGCATCCGCATTATTACCACTATTAAAATTAGTATTAGTTGTTGTCTTTGCAATGGTG
TTCTTTGTATTCGCTATACTAGGACTAGTTGCATGGATGTGTGTTATTAATATCATGAATATTATTAGAATCTTGAAAAG
TGAATTGCTTTTAGCATTTTCTACATCAAGTTCGGAAGCTGTACTTCCTGTAATGATGAAGAAAATGGAAAACTTCGGTT
CTCCAAAAGAAATTACTTCTTTTGTTATACCAATTGGTTATACGTTTAACTTAGATGGATCAGCACTTTATCAATCTATT
GCAGCATTATTCGTTGCACAGATGTATGGAATGCACTTAACATTATCAGAGCAAATTGTGTTGATGTTAACATTAATGAT
TACGTCTAAAGGTATGGCGGCAGTACCAGGAACTTCAATCGTTGTTTTATTAACAACATTAGGTGCCATGGGCTTACCGG
CACAAGGTTTAGCATTAATTATTGGTGTTGACCGTATCTTAGATATGGTACGTACATGTGTAAACGTTATTGGTAATGCA
TTATCAACAATCGTTATAGCTAAATGGGAAAACGTATATGACAAAGCAAAAGGTCAAGAATATTTAAAATCAATTTAA

Upstream 100 bases:

>100_bases
ATTTTTGACATCTATAAAAGATAAATATTTTAATTTTTTATAGATAATTATCAATTTTCTGATATAATTGAGTGATGTCA
GATGAAGGGGAGTTTGTACT

Downstream 100 bases:

>100_bases
AAAATACTATCTGACATTTAAAGCCCCTTACAACCTTTGGTTGTTAGGGGCTTTTATGCATGCGCTAAAGCGCAGGCGAA
CGGAAGCGTAGTGACCTCAG

Product: proton/sodium-glutamate symporter

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate-aspartate carrier protein [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDVFLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWK
TILYFEIITTIAIGLGIIFGNLFKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTIITFGASALLPLLKLVLVVVFAMV
FFVFAILGLVAWMCVINIMNIIRILKSELLLAFSTSSSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSI
AALFVAQMYGMHLTLSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRTCVNVIGNA
LSTIVIAKWENVYDKAKGQEYLKSI

Sequences:

>Translated_425_residues
MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDVFLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWK
TILYFEIITTIAIGLGIIFGNLFKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTIITFGASALLPLLKLVLVVVFAMV
FFVFAILGLVAWMCVINIMNIIRILKSELLLAFSTSSSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSI
AALFVAQMYGMHLTLSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRTCVNVIGNA
LSTIVIAKWENVYDKAKGQEYLKSI
>Mature_424_residues
ALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDVFLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKT
ILYFEIITTIAIGLGIIFGNLFKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELLP
IIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTIITFGASALLPLLKLVLVVVFAMVF
FVFAILGLVAWMCVINIMNIIRILKSELLLAFSTSSSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIA
ALFVAQMYGMHLTLSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRTCVNVIGNAL
STIVIAKWENVYDKAKGQEYLKSI

Specific function: This carrier protein is part of the Na(+)-dependent, binding-protein-independent glutamate-aspartate transport system [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI4827012, Length=470, Percent_Identity=25.7446808510638, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI21314632, Length=428, Percent_Identity=27.5700934579439, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI40254478, Length=447, Percent_Identity=26.6219239373602, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI66773030, Length=453, Percent_Identity=28.0353200883002, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI169790839, Length=446, Percent_Identity=26.2331838565022, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI194239697, Length=479, Percent_Identity=26.5135699373695, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI5032093, Length=430, Percent_Identity=28.3720930232558, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI223468566, Length=274, Percent_Identity=31.7518248175182, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI223468564, Length=268, Percent_Identity=31.7164179104478, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI262359914, Length=410, Percent_Identity=24.390243902439, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI301601644, Length=147, Percent_Identity=38.0952380952381, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1790514, Length=425, Percent_Identity=51.5294117647059, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1789947, Length=426, Percent_Identity=37.3239436619718, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1788024, Length=393, Percent_Identity=25.9541984732824, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789473, Length=272, Percent_Identity=25.3676470588235, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17537407, Length=415, Percent_Identity=30.3614457831325, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71996953, Length=467, Percent_Identity=28.2655246252677, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI193206505, Length=466, Percent_Identity=27.6824034334764, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI71983099, Length=482, Percent_Identity=26.1410788381743, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI71983106, Length=482, Percent_Identity=26.1410788381743, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI193206654, Length=444, Percent_Identity=25.6756756756757, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17541374, Length=406, Percent_Identity=27.3399014778325, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24583025, Length=434, Percent_Identity=26.2672811059908, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17137668, Length=434, Percent_Identity=26.2672811059908, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24583023, Length=434, Percent_Identity=26.2672811059908, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI281360481, Length=461, Percent_Identity=25.5965292841649, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI281360483, Length=461, Percent_Identity=25.5965292841649, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI17137666, Length=461, Percent_Identity=25.5965292841649, Blast_Score=105, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46111; Mature: 45980

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCC
>Mature Secondary Structure 
ALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDVFLNLIKMIVIPVVFCS
CCHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNLFKPGAGLDPTKLPKGDIS
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
KYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELLPIIFFAVFFGLGLAAVGKKA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
EPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTIITFGASALLPLLKLVLVVVFAMV
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
FFVFAILGLVAWMCVINIMNIIRILKSELLLAFSTSSSEAVLPVMMKKMENFGSPKEITS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHH
FVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLTLSEQIVLMLTLMITSKGMAAVPGTSI
HHEECCEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHH
VVLLTTLGAMGLPAQGLALIIGVDRILDMVRTCVNVIGNALSTIVIAKWENVYDKAKGQE
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
YLKSI
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1359385 [H]