Click here to switch to the map view.

The map label for this gene is lacE

Identifier: 151222306

GI number: 151222306

Start: 2332316

End: 2334028

Strand: Reverse

Name: lacE

Synonym: NWMN_2094

Alternate gene names: 151222306

Gene position: 2334028-2332316 (Counterclockwise)

Preceding gene: 151222307

Following gene: 151222305

Centisome position: 81.07

GC content: 35.79

Gene sequence:

>1713_bases
ATGATGCAAAAATTGATCGCACAAATTGAAAAAGGGAAGCCATTTTTCGAGAAATTATCACGTAACATATATTTAAGAGC
CATCCGTGATGGATTTATTTCTGCAATGCCTGTCATCTTATTCTCAAGTATCTTTCTATTAATTGCATATGTGCCAAATA
TATTTGGTTTTAAATGGGATAAAGGCATGGAAGCCATTTTAATGAAACCATATAACTATACGATGGGGCTTGTAGCATTT
TTAGTAGCAGGAACGACAGCGAAATCTTTAACAGATTCATTTAACCGTAAGCTAGAAAGTACAAATCAAATTAACTTTAT
TTCTACAATGCTAGCAGCGATGTGTGGATTCTTGTTCTTAGCTTCTGATCCAGCCAAAGATGGTGGATTCTTAAGTGCAT
TTATGGGAACTAAAGGTTTATTAACAGCATTCTTATCAGCATTTGTAACTGTTATTGTTTATAACTTCTGTGTGAAGCGC
AATATTACAATTAAAATGCCGAAAGAAGTACCGCCGAATATTTCACAAGTATTTAAGGACTTAATTCCATTTTCAGCGGT
AATCATCATTCTTTATGCATTAGATTTAGTCATTCGCAACAGCTTTAAATCAAATGTAGCGGAAGGTATTTTAAAATTAT
TCGAACCATTATTTACAGCAGCAGATGGATGGATTGGTGTCACAATTATCTTTGGTGCCTTTGCATTATTCTGGTTTGTA
GGTATTCATGGTCCGTCAATTGTAGAGCCAGCAATTGCAGCCATTACATATGCGAATATCGAAGCGAACTTCAAGTTGCT
TCAAGCTGGAGAACACGCAGATAAAATTATTACATCAGGTACACAAATGTTTATCGTTACATTTGGAGGTACGGGTGCAA
CATTGGTCGTCCCGTTCATGTTTATGTGGATGACGAAATCTAAACGAAACAAAGCGATTGGACGTGCATCAGTTGTACCA
ACATTCTTTGGTGTAAACGAACCAATTTTATTTGGTGCACCACTAGTATTGAATCCTGTGTTCTTTATTCCATTTGTATT
AGCACCAATTGTTAACGTATGGATTTTCAAACTTTTCGTTGAAGTGTTAGGAATGAATAGTTTTAGTGTGAATTTACCTT
GGACAACACCAGGTCCATTGGGCATTATCATGGGTACAGGTTTTGGTTTATGGTCATTCGTACTAGCTATTACTTTGATT
GTTGTAGATATTATTATTTACTACCCATTCCTAAAAGTTTATGATAGTGAAATTCTTGATGAAGAAGAAGGACGTAAAGA
AAGTAATTCAGATTTAAAAGAAAAAGTTGCAGCAAACTTTGATACGAAAAAAGCTGATTCAATTTTAGCGGCAAGTGGTG
TATCAGACGATGCAGCTAAAGCTTCAAATATCACAGAACAAACAAATGTCTTAGTACTTTGTGCCGGTGGTGGTACAAGT
GGATTATTAGCCAATGCACTTAATAAAGCAGCTGAAGAATATCATGTGCCAGTGAAAGCGGCAGCTGGCGGATATGGCGC
ACATATGGACATTATGAAAGAGTACCAATTAATCATTTTAGCGCCACAAGTTGCGTCTAACTATGAAGATATTAAGCAAG
ATACAGATAGATTAGGTATTAAATTAGCGAAGACTCAAGGTGCTGAATATATCAAATTGACACGAGATGGCCAAGCAGCC
CTTGATTTCGTTCAACAACAATTTGAAAATTAA

Upstream 100 bases:

>100_bases
GTGTAACGATGATGCATGGTCAAGATCATTTAATGACAACAATTTTACTGAAAGATTTAATGAAGCATTTATTAGAATTT
TATAAAAGAGGGTGATGACA

Downstream 100 bases:

>100_bases
GAATAGGAGTTTTTCATATGACTAAAACATTACCTGAAGATTTTATTTTTGGTGGAGCAACAGCAGCATATCAAGCAGAA
GGTGCGACAAATACGGATGG

Product: PTS system, lactose-specific IIBC component

Products: NA

Alternate protein names: EIICB-Lac; EII-Lac; Lactose permease IIC component; PTS system lactose-specific EIIC component; Lactose-specific phosphotransferase enzyme IIB component; PTS system lactose-specific EIIB component

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSIFLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAF
LVAGTTAKSLTDSFNRKLESTNQINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLFTAADGWIGVTIIFGAFALFWFV
GIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKIITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVP
TFFGVNEPILFGAPLVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWSFVLAITLI
VVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILAASGVSDDAAKASNITEQTNVLVLCAGGGTS
GLLANALNKAAEEYHVPVKAAAGGYGAHMDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAA
LDFVQQQFEN

Sequences:

>Translated_570_residues
MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSIFLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAF
LVAGTTAKSLTDSFNRKLESTNQINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLFTAADGWIGVTIIFGAFALFWFV
GIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKIITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVP
TFFGVNEPILFGAPLVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWSFVLAITLI
VVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILAASGVSDDAAKASNITEQTNVLVLCAGGGTS
GLLANALNKAAEEYHVPVKAAAGGYGAHMDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAA
LDFVQQQFEN
>Mature_570_residues
MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSIFLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAF
LVAGTTAKSLTDSFNRKLESTNQINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLFTAADGWIGVTIIFGAFALFWFV
GIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKIITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVP
TFFGVNEPILFGAPLVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWSFVLAITLI
VVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILAASGVSDDAAKASNITEQTNVLVLCAGGGTS
GLLANALNKAAEEYHVPVKAAAGGYGAHMDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAA
LDFVQQQFEN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain

Homologues:

Organism=Escherichia coli, GI1788032, Length=477, Percent_Identity=26.8343815513627, Blast_Score=122, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTLCB_STAAC (Q5HE15)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_186992.1
- ProteinModelPortal:   Q5HE15
- SMR:   Q5HE15
- STRING:   Q5HE15
- EnsemblBacteria:   EBSTAT00000010740
- GeneID:   3238567
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL2181
- TIGR:   SACOL2181
- eggNOG:   COG1455
- GeneTree:   EBGT00050000025328
- HOGENOM:   HBG337916
- OMA:   WSKDIEN
- ProtClustDB:   CLSK877288
- BioCyc:   SAUR93062:SACOL2181-MONOMER
- InterPro:   IPR014350
- InterPro:   IPR003501
- InterPro:   IPR013012
- InterPro:   IPR003352
- InterPro:   IPR004501
- InterPro:   IPR004801
- TIGRFAMs:   TIGR00394
- TIGRFAMs:   TIGR00410
- TIGRFAMs:   TIGR00853

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB

EC number: =2.7.1.69

Molecular weight: Translated: 62433; Mature: 62433

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS51100 PTS_EIIB_TYPE_3; PS51105 PTS_EIIC_TYPE_3

Important sites: ACT_SITE 474-474

Signals:

None

Transmembrane regions:

HASH(0x405b6028)-; HASH(0x1a01466c)-; HASH(0x1b60eac0)-; HASH(0x1b68bc90)-; HASH(0x1b69cbe0)-; HASH(0x1ac85528)-; HASH(0x1aa1ad38)-; HASH(0x1ac9a860)-; HASH(0x1b25e4e8)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEECCCCCCEEEEECCCHHHHHHHHHHHCH
>Mature Secondary Structure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EEECCCCCCEEEEECCCHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA