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The map label for this gene is bsaD

Identifier: 151221925

GI number: 151221925

Start: 1909764

End: 1910297

Strand: Reverse

Name: bsaD

Synonym: NWMN_1713

Alternate gene names: 151221925

Gene position: 1910297-1909764 (Counterclockwise)

Preceding gene: 151221926

Following gene: 151221924

Centisome position: 66.36

GC content: 27.34

Gene sequence:

>534_bases
ATGGAGGCGTATATCATGGGAGAAAATGTATTGATTTGTTTGTGTGGCTCAGTTAATAGTATCAATATTAGCCACTATAT
TATTGAATTAAAATCAAAATTTGATGAAGTAAATGTAATCGCGTCAACAAACGGTAGAAAGTTTATTAATGGTGAAATAT
TAAAGCAATTTTGCGATAACTATTATGACGAATTTGAAGACCCTTTTTTAAATCATGTAGATATAGCAAATAAACATGAC
AAGATTATTATTTTACCTGCGACTTCTAATACGATTAATAAAATTGCAAATGGTATATGTGACAATTTATTATTAACTAT
TTGTCATACAGCTTTTGAAAAACTTTCTATATTTCCAAATATGAATTTACGAATGTGGGAAAATCCAGTTACTCAAAATA
ATATTCGATTATTAAAAGATTATGGTGTATCAATATATCCAGCAAATATTTCAGAAAGTTATGAATTAGCGTCAAAAACA
TTTAAAAAGAATGTTGTCGCACCTGAACCATATAAAGTTCTGGAATTCATTTGA

Upstream 100 bases:

>100_bases
TTTAGAAGGTGAATTAGGGGTCTATCTAACATTGATGACACTGAAAAATACAAAAATTCTCAATGAAAAAAATTGGACCA
ATGCGTTTTTAATTAGCTGA

Downstream 100 bases:

>100_bases
GATTAGAATATGAAAATCATAAAGCGCGCTATTATTAGTTTAATAATATTGAGTCTTTTAATATCTATTACAATGAGTAA
TGCATCAGCGTCAGAAGAAC

Product: lantibiotic modifying enzyme

Products: N-(R)-4-Phosphopantothenoyl)-L-cysteine; CMP; H+; Diphosphate [C]

Alternate protein names: Epidermin-modifying enzyme epiD [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MEAYIMGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFINGEILKQFCDNYYDEFEDPFLNHVDIANKHD
KIIILPATSNTINKIANGICDNLLLTICHTAFEKLSIFPNMNLRMWENPVTQNNIRLLKDYGVSIYPANISESYELASKT
FKKNVVAPEPYKVLEFI

Sequences:

>Translated_177_residues
MEAYIMGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFINGEILKQFCDNYYDEFEDPFLNHVDIANKHD
KIIILPATSNTINKIANGICDNLLLTICHTAFEKLSIFPNMNLRMWENPVTQNNIRLLKDYGVSIYPANISESYELASKT
FKKNVVAPEPYKVLEFI
>Mature_177_residues
MEAYIMGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFINGEILKQFCDNYYDEFEDPFLNHVDIANKHD
KIIILPATSNTINKIANGICDNLLLTICHTAFEKLSIFPNMNLRMWENPVTQNNIRLLKDYGVSIYPANISESYELASKT
FKKNVVAPEPYKVLEFI

Specific function: Catalyzes the removal of two reducing equivalents (oxidative decarboxylation) from the cysteine residue of the C- terminal meso-lanthionine of epidermin to form a --C==C-- double bond [H]

COG id: COG0452

COG function: function code H; Phosphopantothenoylcysteine synthetase/decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily [H]

Homologues:

Organism=Homo sapiens, GI71725351, Length=147, Percent_Identity=31.2925170068027, Blast_Score=71, Evalue=4e-13,
Organism=Escherichia coli, GI87082301, Length=147, Percent_Identity=22.4489795918367, Blast_Score=72, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24657262, Length=155, Percent_Identity=30.3225806451613, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003382 [H]

Pfam domain/function: PF02441 Flavoprotein [H]

EC number: 4.1.1.36; 6.3.2.5 [C]

Molecular weight: Translated: 20202; Mature: 20202

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAYIMGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFINGEILKQFCDN
CCEEEECCCEEEEEECCCCEEEHHHHEEEEHHCCCEEEEEEECCCCEEECHHHHHHHHHH
YYDEFEDPFLNHVDIANKHDKIIILPATSNTINKIANGICDNLLLTICHTAFEKLSIFPN
HHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MNLRMWENPVTQNNIRLLKDYGVSIYPANISESYELASKTFKKNVVAPEPYKVLEFI
CCEEEECCCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHCC
>Mature Secondary Structure
MEAYIMGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFINGEILKQFCDN
CCEEEECCCEEEEEECCCCEEEHHHHEEEEHHCCCEEEEEEECCCCEEECHHHHHHHHHH
YYDEFEDPFLNHVDIANKHDKIIILPATSNTINKIANGICDNLLLTICHTAFEKLSIFPN
HHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MNLRMWENPVTQNNIRLLKDYGVSIYPANISESYELASKTFKKNVVAPEPYKVLEFI
CCEEEECCCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-4'-Phosphopantothenate; CTP; L-Cysteine [C]

Specific reaction: D-4'-Phosphopantothenate + CTP + L-Cysteine --> N-((R)-4-Phosphopantothenoyl)-L-cysteine + CMP + H+ + Diphosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1740156; 1644762; 11101502 [H]