BacMap

Definition	Sinorhizobium medicae WSM419 chromosome, complete genome.
Accession	NC_009636
Length	3,781,904

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The map label for this gene is fadB1 [H]

Identifier: 150398757

GI number: 150398757

Start: 3781073

End: 3781846

Strand: Direct

Name: fadB1 [H]

Synonym: Smed_3574

Alternate gene names: 150398757

Gene position: 3781073-3781846 (Clockwise)

Preceding gene: 150398756

Following gene: NA

Centisome position: 99.98

GC content: 65.76

Gene sequence:

>774_bases
ATGGGTTACGAGACGTTGCTGGTGGAAACGCGGGGCCGCGTGGGCCTGATTACGCTCAACCGACCCCAGGCGCTCAATGC
GCTGAATGCGGCGGTTATGCGCGACCTCCATGAAGCGCTGAACGCCTTCGACGCCGACAAGGGCGTCGGCGCCATCGTTC
TTACCGGCTCCGAAAAGGCATTTGCCGCCGGGGCCGACGTCAAGGAGATGCAGGCGCTCGATTTCGTCGAGGGTTATCTC
GGCGACTTCCTCGGCGGCTGGGAGCGTGTCGCGGACGCCCGCAAGCCTATGATTGCGGCGGTCTCGGGCTATGCGCTCGG
CGGCGGCTGCGAACTCGCCATGATGTGCGACCTCATCATCGCTTCCGAAACGGCGAAGTTCGGACAGCCGGAGATCACGC
TTGGCGTTATTCCCGGCATGGGTGGGTCGCAGCGGCTGACCCGCGCCATCGGCAAGGCGAAGGCGATGGACCTCATCCTC
ACCGGCCGCATGATGGACGCGGCGGAGGCCGAGCGCTCCGGGCTGGTGTCGCGCGTGGTGGCTCCCGACAGGCTCCTGGA
GGAGGCGCTCGGCGCCGCCGAAAAGATCGCATCCTTCTCGCTGCCGGCGGCGATGATGGCCAAGGAGGCGGTCAGCCGCT
CTCTGGAGCTGACGCTGGCCGAGGGGCTGCGTTTCGAGCGGCGGCTCTTCCAGTCGCTCTTCGCGACGGAGGATCAGAAA
GAGGGCATGGGCGCCTTCGTCGCCAAGCGCAAAGCCGAATTCAAAAACAAGTGA

Upstream 100 bases:

>100_bases
CTTTTACTGTCCGCACTGCCAGAAATAGCGCGCGGCAAGGCACATTTCGCTCCCGGATCTGCCGGGCGCTCGATTCCGAA
AATGCGTGAAGGAGACGGGA

Downstream 100 bases:

>100_bases
GGCACGGGTGGCACGCGGTTTTCTCAAATTCCGCGTTGACGTAGGGGCGGTTTCCCTTTATACGCCGCCCTCAGTTTGGA
ACGCACTCGTTCGAGGCTTT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MGYETLLVETRGRVGLITLNRPQALNALNAAVMRDLHEALNAFDADKGVGAIVLTGSEKAFAAGADVKEMQALDFVEGYL
GDFLGGWERVADARKPMIAAVSGYALGGGCELAMMCDLIIASETAKFGQPEITLGVIPGMGGSQRLTRAIGKAKAMDLIL
TGRMMDAAEAERSGLVSRVVAPDRLLEEALGAAEKIASFSLPAAMMAKEAVSRSLELTLAEGLRFERRLFQSLFATEDQK
EGMGAFVAKRKAEFKNK

Sequences:

>Translated_257_residues
MGYETLLVETRGRVGLITLNRPQALNALNAAVMRDLHEALNAFDADKGVGAIVLTGSEKAFAAGADVKEMQALDFVEGYL
GDFLGGWERVADARKPMIAAVSGYALGGGCELAMMCDLIIASETAKFGQPEITLGVIPGMGGSQRLTRAIGKAKAMDLIL
TGRMMDAAEAERSGLVSRVVAPDRLLEEALGAAEKIASFSLPAAMMAKEAVSRSLELTLAEGLRFERRLFQSLFATEDQK
EGMGAFVAKRKAEFKNK
>Mature_256_residues
GYETLLVETRGRVGLITLNRPQALNALNAAVMRDLHEALNAFDADKGVGAIVLTGSEKAFAAGADVKEMQALDFVEGYLG
DFLGGWERVADARKPMIAAVSGYALGGGCELAMMCDLIIASETAKFGQPEITLGVIPGMGGSQRLTRAIGKAKAMDLILT
GRMMDAAEAERSGLVSRVVAPDRLLEEALGAAEKIASFSLPAAMMAKEAVSRSLELTLAEGLRFERRLFQSLFATEDQKE
GMGAFVAKRKAEFKNK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=259, Percent_Identity=55.5984555984556, Blast_Score=294, Evalue=4e-80,
Organism=Homo sapiens, GI68989263, Length=226, Percent_Identity=34.070796460177, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=36.71875, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI70995211, Length=255, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI31542718, Length=248, Percent_Identity=29.0322580645161, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI20127408, Length=180, Percent_Identity=34.4444444444444, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI150378541, Length=246, Percent_Identity=32.520325203252, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI37594469, Length=213, Percent_Identity=31.924882629108, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI37594471, Length=213, Percent_Identity=31.924882629108, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI25453481, Length=266, Percent_Identity=25.187969924812, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI51538573, Length=266, Percent_Identity=25.187969924812, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI4757968, Length=268, Percent_Identity=24.2537313432836, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI49355787, Length=268, Percent_Identity=24.2537313432836, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI4757966, Length=255, Percent_Identity=25.0980392156863, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI51538581, Length=255, Percent_Identity=25.0980392156863, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI157694516, Length=239, Percent_Identity=27.6150627615063, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI213417737, Length=236, Percent_Identity=26.6949152542373, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI45643119, Length=223, Percent_Identity=28.2511210762332, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI260275230, Length=223, Percent_Identity=28.2511210762332, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI260274832, Length=223, Percent_Identity=28.2511210762332, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI221136756, Length=241, Percent_Identity=26.1410788381743, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI221136753, Length=241, Percent_Identity=26.1410788381743, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI221307494, Length=241, Percent_Identity=26.1410788381743, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI261878539, Length=135, Percent_Identity=35.5555555555556, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI116235487, Length=184, Percent_Identity=28.804347826087, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI62530384, Length=226, Percent_Identity=26.9911504424779, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI157694520, Length=214, Percent_Identity=27.1028037383178, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=252, Percent_Identity=46.8253968253968, Blast_Score=218, Evalue=2e-58,
Organism=Escherichia coli, GI221142681, Length=263, Percent_Identity=36.1216730038023, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1788597, Length=273, Percent_Identity=31.5018315018315, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1790281, Length=178, Percent_Identity=35.3932584269663, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=32.6996197718631, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=31.5384615384615, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788682, Length=208, Percent_Identity=32.2115384615385, Blast_Score=88, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=56.2753036437247, Blast_Score=280, Evalue=5e-76,
Organism=Caenorhabditis elegans, GI25145438, Length=242, Percent_Identity=48.3471074380165, Blast_Score=234, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17540714, Length=227, Percent_Identity=34.8017621145374, Blast_Score=124, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17560910, Length=254, Percent_Identity=35.4330708661417, Blast_Score=120, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17540306, Length=243, Percent_Identity=31.2757201646091, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17558304, Length=187, Percent_Identity=34.7593582887701, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17536985, Length=272, Percent_Identity=27.2058823529412, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI25144160, Length=245, Percent_Identity=31.4285714285714, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25144157, Length=245, Percent_Identity=31.4285714285714, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25144276, Length=196, Percent_Identity=33.6734693877551, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17508951, Length=196, Percent_Identity=33.6734693877551, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17508953, Length=196, Percent_Identity=33.6734693877551, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17534483, Length=251, Percent_Identity=26.2948207171315, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=242, Percent_Identity=28.099173553719, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=241, Percent_Identity=26.5560165975104, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=190, Percent_Identity=26.3157894736842, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=199, Percent_Identity=30.6532663316583, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI20129971, Length=258, Percent_Identity=56.9767441860465, Blast_Score=292, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24653477, Length=258, Percent_Identity=56.9767441860465, Blast_Score=292, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=35.7142857142857, Blast_Score=138, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24650670, Length=213, Percent_Identity=38.4976525821596, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21357171, Length=249, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19920382, Length=266, Percent_Identity=32.3308270676692, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=28.3524904214559, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24654903, Length=188, Percent_Identity=29.2553191489362, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI28571730, Length=181, Percent_Identity=32.5966850828729, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI28571729, Length=181, Percent_Identity=32.5966850828729, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19921000, Length=174, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24583077, Length=174, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24583079, Length=174, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24583165, Length=173, Percent_Identity=26.5895953757225, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921018, Length=196, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27392; Mature: 27261

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYETLLVETRGRVGLITLNRPQALNALNAAVMRDLHEALNAFDADKGVGAIVLTGSEKA
CCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
FAAGADVKEMQALDFVEGYLGDFLGGWERVADARKPMIAAVSGYALGGGCELAMMCDLII
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHCCEEECCCCHHHHHHHHHH
ASETAKFGQPEITLGVIPGMGGSQRLTRAIGKAKAMDLILTGRMMDAAEAERSGLVSRVV
HHCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
APDRLLEEALGAAEKIASFSLPAAMMAKEAVSRSLELTLAEGLRFERRLFQSLFATEDQK
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EGMGAFVAKRKAEFKNK
CCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GYETLLVETRGRVGLITLNRPQALNALNAAVMRDLHEALNAFDADKGVGAIVLTGSEKA
CCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
FAAGADVKEMQALDFVEGYLGDFLGGWERVADARKPMIAAVSGYALGGGCELAMMCDLII
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHCCEEECCCCHHHHHHHHHH
ASETAKFGQPEITLGVIPGMGGSQRLTRAIGKAKAMDLILTGRMMDAAEAERSGLVSRVV
HHCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
APDRLLEEALGAAEKIASFSLPAAMMAKEAVSRSLELTLAEGLRFERRLFQSLFATEDQK
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EGMGAFVAKRKAEFKNK
CCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]