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The map label for this gene is gshB [H]

Identifier: 150398730

GI number: 150398730

Start: 3751749

End: 3752696

Strand: Direct

Name: gshB [H]

Synonym: Smed_3546

Alternate gene names: 150398730

Gene position: 3751749-3752696 (Clockwise)

Preceding gene: 150398729

Following gene: 150398731

Centisome position: 99.2

GC content: 62.87

Gene sequence:

>948_bases
ATGGCAGGAATCGTCAATGTCGGGGTCCAGATGGACCATGTTTCGGGCATCACCATTGCGGGCGATTCGACCTTCGCGAT
GAGCCTGGAGGCACAGGCCCGCGGCTACCGCCTGTTCCACTACACGCCCGACAAGCTGTCGCTTCGGGACGGCAAGGTCT
ATGCGACCGCGCAGCAGATGCAGCTGCGCGACGTCAAGGGCGATCACTTCACCCTCGGCGAGGACGAGCGGATCGACCTT
TCGACTCTGGACGTCATCCTGCTTCGCCAGGATCCGCCCTTCGACATGGCCTATATCACCTCCACCCATCTGCTCGAGCG
TCTCCACCCCAAGACCTTGGTCGTCAACGATCCGGCCTGGGTACGGAACTCTCCGGAAAAGATCTTCGTGACCGAATTTG
CGGACCTGATGCCGAAGACGCTGATCACCCGCGATGCGGACGAGATTGCCCGCTTCCGCCAGGAAATGGGGGACATCATC
CTGAAGCCGCTTTACGGCAATGGCGGCGCGGGGGTCTTCCACTCGGCGCGCGACGACCGCAATTTCTCGTCGCTCCTCGA
AATGTTCGCCCAGATGTTCCGCGAGCCTTACATTGCCCAGGAATACCTGCCGGAGGTCCGCAAGGGCGACAAGCGCATCC
TGCTCGTCGATGGCGAGCCCGTCGGCGCGATCAATCGCGTCCCGGCCGAGCACGACTCGCGCTCCAACATGCATGCTGGC
GGGCGCGCGGAGGCAACCGAGCTGACGGCGCGCGAAAGGGAAATCTGCACCCGTATCGGGCCGGCGCTGAAAGAACGCGG
CTTCCTCTTCGTCGGCATCGACGTCATCGGCGACTATCTGACGGAGATCAACGTCACCTCGCCCACCGGCATCCGCGAAG
TGAAGAAGTTCGGCGGCGCCGATGTCGCCGGTCTTCTCTGGGACGCGATCGAGAAAAAGCGCGCCTGA

Upstream 100 bases:

>100_bases
ACAGGCGCAGCATCGCGTTGAAGTGCCGTATAATTCGATTTCCGGCCGACACCGGCCGAAGGAACTATGCGGATCGAAGA
TCCAGATCAGGAATGGGAAG

Downstream 100 bases:

>100_bases
TTCGCTCGGCGGGCCTTCATCCTTCATTGTTCTGTAATTGTTCTTGTTTTTAAGCGCATTCCGTGCAAGGTTTGCCGATG
CAACCGAATTCGGTTGTGAC

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MAGIVNVGVQMDHVSGITIAGDSTFAMSLEAQARGYRLFHYTPDKLSLRDGKVYATAQQMQLRDVKGDHFTLGEDERIDL
STLDVILLRQDPPFDMAYITSTHLLERLHPKTLVVNDPAWVRNSPEKIFVTEFADLMPKTLITRDADEIARFRQEMGDII
LKPLYGNGGAGVFHSARDDRNFSSLLEMFAQMFREPYIAQEYLPEVRKGDKRILLVDGEPVGAINRVPAEHDSRSNMHAG
GRAEATELTAREREICTRIGPALKERGFLFVGIDVIGDYLTEINVTSPTGIREVKKFGGADVAGLLWDAIEKKRA

Sequences:

>Translated_315_residues
MAGIVNVGVQMDHVSGITIAGDSTFAMSLEAQARGYRLFHYTPDKLSLRDGKVYATAQQMQLRDVKGDHFTLGEDERIDL
STLDVILLRQDPPFDMAYITSTHLLERLHPKTLVVNDPAWVRNSPEKIFVTEFADLMPKTLITRDADEIARFRQEMGDII
LKPLYGNGGAGVFHSARDDRNFSSLLEMFAQMFREPYIAQEYLPEVRKGDKRILLVDGEPVGAINRVPAEHDSRSNMHAG
GRAEATELTAREREICTRIGPALKERGFLFVGIDVIGDYLTEINVTSPTGIREVKKFGGADVAGLLWDAIEKKRA
>Mature_314_residues
AGIVNVGVQMDHVSGITIAGDSTFAMSLEAQARGYRLFHYTPDKLSLRDGKVYATAQQMQLRDVKGDHFTLGEDERIDLS
TLDVILLRQDPPFDMAYITSTHLLERLHPKTLVVNDPAWVRNSPEKIFVTEFADLMPKTLITRDADEIARFRQEMGDIIL
KPLYGNGGAGVFHSARDDRNFSSLLEMFAQMFREPYIAQEYLPEVRKGDKRILLVDGEPVGAINRVPAEHDSRSNMHAGG
RAEATELTAREREICTRIGPALKERGFLFVGIDVIGDYLTEINVTSPTGIREVKKFGGADVAGLLWDAIEKKRA

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=312, Percent_Identity=43.2692307692308, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI1787076, Length=182, Percent_Identity=30.7692307692308, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35206; Mature: 35074

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGIVNVGVQMDHVSGITIAGDSTFAMSLEAQARGYRLFHYTPDKLSLRDGKVYATAQQM
CCCEEECCEEEECCCCEEEECCCEEEEEEEECCCCEEEEEECCCCCEECCCEEEEEHHHH
QLRDVKGDHFTLGEDERIDLSTLDVILLRQDPPFDMAYITSTHLLERLHPKTLVVNDPAW
HHHHCCCCEEECCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCHH
VRNSPEKIFVTEFADLMPKTLITRDADEIARFRQEMGDIILKPLYGNGGAGVFHSARDDR
HCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCC
NFSSLLEMFAQMFREPYIAQEYLPEVRKGDKRILLVDGEPVGAINRVPAEHDSRSNMHAG
CHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GRAEATELTAREREICTRIGPALKERGFLFVGIDVIGDYLTEINVTSPTGIREVKKFGGA
CCCHHHHHHHHHHHHHHHHCHHHHHCCEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHCCC
DVAGLLWDAIEKKRA
CHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGIVNVGVQMDHVSGITIAGDSTFAMSLEAQARGYRLFHYTPDKLSLRDGKVYATAQQM
CCEEECCEEEECCCCEEEECCCEEEEEEEECCCCEEEEEECCCCCEECCCEEEEEHHHH
QLRDVKGDHFTLGEDERIDLSTLDVILLRQDPPFDMAYITSTHLLERLHPKTLVVNDPAW
HHHHCCCCEEECCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCHH
VRNSPEKIFVTEFADLMPKTLITRDADEIARFRQEMGDIILKPLYGNGGAGVFHSARDDR
HCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCC
NFSSLLEMFAQMFREPYIAQEYLPEVRKGDKRILLVDGEPVGAINRVPAEHDSRSNMHAG
CHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GRAEATELTAREREICTRIGPALKERGFLFVGIDVIGDYLTEINVTSPTGIREVKKFGGA
CCCHHHHHHHHHHHHHHHHCHHHHHCCEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHCCC
DVAGLLWDAIEKKRA
CHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]