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The map label for this gene is pyrF [H]

Identifier: 150398724

GI number: 150398724

Start: 3746754

End: 3747452

Strand: Reverse

Name: pyrF [H]

Synonym: Smed_3540

Alternate gene names: 150398724

Gene position: 3747452-3746754 (Counterclockwise)

Preceding gene: 150398725

Following gene: 150398723

Centisome position: 99.09

GC content: 64.23

Gene sequence:

>699_bases
ATGAGCGCACGCGACCGGCTGATCGTCGGCCTTGATCTTCCAACGCTGGCCGAGGCCGAAAAAATCGTCTCGGCGCTCGG
TGAGGAAGTCCTCTTCTATAAGATAGGCTACCAACTGGCATTCGCTGGCGGTCTCGACTTCGCGCGCGATCTCGCCGCGA
GCGGCAAGCAGGTCTTTCTGGACATGAAGCTGCTCGACATCGACAACACGGTCGCGAAGGGCGTGGAGAACATCGTCAAG
ATGGGCGTGTCGATGCTGACGCTCCATGCCTATCCCAAGGCGATGAAATCAGCCGTCGAGGCGGCCAGAGGCTCAAACCT
CTGCCTGCTTGCAGTGACGGTGCTCACCTCCATGGACGAGCAGGACGTGATGGATGCCGGCTACAATTATGATCCGCAAA
GCCTGGTCCTGCGCCGTGCCGAGCAGGCACACGCCGCGGGCATGGGAGGCATTGTCTGCTCGGCGGCGGAGGCCGCCGCG
GTGCGCAAGATCATTGGCGGCGACATGGCGCTGGTGACACCGGGCATCCGCCCGGCCGGCGCGGAAAAAGGCGACCAGAA
GCGGGTGATGACGCCGGCCGATGCGCTTGCGGCCGGCTCGAGCCATCTCGTCGTCGGGCGGCCAATCGTTGCCGCGCCTG
ACCCTCTTGCAGCAAGCCGCGCGATTCTGGCCGAGATGGAGAGCGCCCTTTCCGGCTGA

Upstream 100 bases:

>100_bases
CAGTTGCCGGGGGGCGGCAATCTCTTCGCCTTCCGTCTTGCGGATCGCGCCGTCACATGCGACTGGACGCCGATGGAAAC
CTGGCAGGGGTAGACTCGAT

Downstream 100 bases:

>100_bases
TTGGCGCATACCCCACCGGACTGCGTCCGGCATGCTTGACCCCGGACACAATTTGCCGGAAGTAACTATCCCGTCTGCAT
TTCGCTGATGACGAGGAGCT

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MSARDRLIVGLDLPTLAEAEKIVSALGEEVLFYKIGYQLAFAGGLDFARDLAASGKQVFLDMKLLDIDNTVAKGVENIVK
MGVSMLTLHAYPKAMKSAVEAARGSNLCLLAVTVLTSMDEQDVMDAGYNYDPQSLVLRRAEQAHAAGMGGIVCSAAEAAA
VRKIIGGDMALVTPGIRPAGAEKGDQKRVMTPADALAAGSSHLVVGRPIVAAPDPLAASRAILAEMESALSG

Sequences:

>Translated_232_residues
MSARDRLIVGLDLPTLAEAEKIVSALGEEVLFYKIGYQLAFAGGLDFARDLAASGKQVFLDMKLLDIDNTVAKGVENIVK
MGVSMLTLHAYPKAMKSAVEAARGSNLCLLAVTVLTSMDEQDVMDAGYNYDPQSLVLRRAEQAHAAGMGGIVCSAAEAAA
VRKIIGGDMALVTPGIRPAGAEKGDQKRVMTPADALAAGSSHLVVGRPIVAAPDPLAASRAILAEMESALSG
>Mature_231_residues
SARDRLIVGLDLPTLAEAEKIVSALGEEVLFYKIGYQLAFAGGLDFARDLAASGKQVFLDMKLLDIDNTVAKGVENIVKM
GVSMLTLHAYPKAMKSAVEAARGSNLCLLAVTVLTSMDEQDVMDAGYNYDPQSLVLRRAEQAHAAGMGGIVCSAAEAAAV
RKIIGGDMALVTPGIRPAGAEKGDQKRVMTPADALAAGSSHLVVGRPIVAAPDPLAASRAILAEMESALSG

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=228, Percent_Identity=38.1578947368421, Blast_Score=145, Evalue=2e-36,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 24124; Mature: 23993

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARDRLIVGLDLPTLAEAEKIVSALGEEVLFYKIGYQLAFAGGLDFARDLAASGKQVFL
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHEEHHHCCEEEEECCCHHHHHHHCCCCEEEE
DMKLLDIDNTVAKGVENIVKMGVSMLTLHAYPKAMKSAVEAARGSNLCLLAVTVLTSMDE
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCH
QDVMDAGYNYDPQSLVLRRAEQAHAAGMGGIVCSAAEAAAVRKIIGGDMALVTPGIRPAG
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
AEKGDQKRVMTPADALAAGSSHLVVGRPIVAAPDPLAASRAILAEMESALSG
CCCCCCCCCCCCHHHHHCCCCCEEECCCCEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SARDRLIVGLDLPTLAEAEKIVSALGEEVLFYKIGYQLAFAGGLDFARDLAASGKQVFL
CCCCCEEEEECCCCHHHHHHHHHHHHHHHEEHHHCCEEEEECCCHHHHHHHCCCCEEEE
DMKLLDIDNTVAKGVENIVKMGVSMLTLHAYPKAMKSAVEAARGSNLCLLAVTVLTSMDE
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCH
QDVMDAGYNYDPQSLVLRRAEQAHAAGMGGIVCSAAEAAAVRKIIGGDMALVTPGIRPAG
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
AEKGDQKRVMTPADALAAGSSHLVVGRPIVAAPDPLAASRAILAEMESALSG
CCCCCCCCCCCCHHHHHCCCCCEEECCCCEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10972799; 11481430 [H]