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The map label for this gene is rsmB [H]

Identifier: 150398714

GI number: 150398714

Start: 3738783

End: 3740072

Strand: Reverse

Name: rsmB [H]

Synonym: Smed_3530

Alternate gene names: 150398714

Gene position: 3740072-3738783 (Counterclockwise)

Preceding gene: 150398715

Following gene: 150398713

Centisome position: 98.89

GC content: 63.72

Gene sequence:

>1290_bases
ATGCGATTGGGCGGAAGGCTCTCGGGAGCCATCGAGGTTCTTGACGATATCGAAAAGCGCAAGCGACCCGTCGCCGATGC
GCTCAGGGACTGGGGCCTGTCCCACCGCTTCGCCGGCTCCGGCGATCGAGCCGCGATAGGCAATATCGTCTACGACGCCC
TGCGGATGAAACTCTCCCACGCCTATCTCATGGACAGCGACAGTCCCGTTGCTCTCGCCCATGCGGTCCTCTACCGGCAA
TGGGGCTTTGACGTCGACACGCTTGCCGCGGAATTTGCCGACGACAGGTTCGCGCCCGAGCCGCCTTCCGAAGCCATGAT
GACGGCTTTCGATCAACGACGGCTCGAAGATGCGCCTCTGCATGTGCGGGCCGATATTCCGGAATGGACGCAAGCCTCGT
TTGAAGAAAACTTCTCCGACGACTGGCTCGCCGAGGCGCAAGCGCTTGCCGGAAGGCCGACTCTGGACCTGCGCGTCAAC
ACACTGAAAGCGACGCGGGAAAAGGTATTGAAGGCGCTGGAGCGCAGCGGCGCCGAGCCTGCAACGATTGCCCGGCACGG
CGTGCGCATCCCCGCTGGCGAGGGCGCTTCGCGCCTGCCGAACGTTACGGCGGAAGTCTCGTTCCAGAAGGGTTGGTTCG
AGGTTCAGGACGAAGGCTCGCAGATCGTCGCTGATCTCGTATTCCCGCGCGAGGGTGAGCAGGTGCTCGATTATTGCGCC
GGCGGCGGCGGCAAGACGCTGGCCATCTCGGCGGCGATGAACAACAAGGGGCAGGTCCACGCTTACGACGCCGACCGCAA
ACGGCTGGCACCGATCATCGAGCGGCTGAAGCGTGCCGGCACCAGAAATGTCCAGGTGCATGAAACGGCCGGTTCACTTG
CGTCCCTCGTCGGTCGTGCCGATCGCGTGCTCGTCGATGCGCCGTGCACCGGAACCGGAACCTGGCGGCGCCGTCCGGAT
ACGAAATGGCGCCTGACGCAGAAGAACCTCGAGGAGCGATTGGCCCAGCAGGAAGAGGCGCTCGCCGGCGCCGCCCGGTT
CGTGCGTCCCGGCGGACATCTGATCTATGTCACCTGTTCCGTGCTTCCGGAGGAGAACGAGTCGCAGGTCTACGGTTTCT
GCGACGACAATCCGGAATTCGAGGTGCTTTCGGCCGCCGACAACTGGGCAGCCCTCTTCGGCACCGACAGGCCGCAACCC
TGGTCCGCCGATATGAAGACCGTGACGCTGACGCCGGCTTCAACCGGAACGGACGGCTTTTTCTTCTGCCTCATGGGGCG
GAAAAACTAG

Upstream 100 bases:

>100_bases
ACCAGACTGCAAGAGCCAACCACCCTGCCACATAATGCGGTCGCCGTCTTGCTCTTCGATGTCATCGCCGTTACCACGGC
GAAAAAGGAGTTCATCGAAG

Downstream 100 bases:

>100_bases
CGTTTGCGTCTCCTGGGGCGGCGCTGCAACTTCAAGCCATTCTAAACTATACACTTTGACACCAGTTTGCTTTTGGTCCA
TGACTCTTGCGCTTTCACGG

Product: Fmu (Sun) domain-containing protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MRLGGRLSGAIEVLDDIEKRKRPVADALRDWGLSHRFAGSGDRAAIGNIVYDALRMKLSHAYLMDSDSPVALAHAVLYRQ
WGFDVDTLAAEFADDRFAPEPPSEAMMTAFDQRRLEDAPLHVRADIPEWTQASFEENFSDDWLAEAQALAGRPTLDLRVN
TLKATREKVLKALERSGAEPATIARHGVRIPAGEGASRLPNVTAEVSFQKGWFEVQDEGSQIVADLVFPREGEQVLDYCA
GGGGKTLAISAAMNNKGQVHAYDADRKRLAPIIERLKRAGTRNVQVHETAGSLASLVGRADRVLVDAPCTGTGTWRRRPD
TKWRLTQKNLEERLAQQEEALAGAARFVRPGGHLIYVTCSVLPEENESQVYGFCDDNPEFEVLSAADNWAALFGTDRPQP
WSADMKTVTLTPASTGTDGFFFCLMGRKN

Sequences:

>Translated_429_residues
MRLGGRLSGAIEVLDDIEKRKRPVADALRDWGLSHRFAGSGDRAAIGNIVYDALRMKLSHAYLMDSDSPVALAHAVLYRQ
WGFDVDTLAAEFADDRFAPEPPSEAMMTAFDQRRLEDAPLHVRADIPEWTQASFEENFSDDWLAEAQALAGRPTLDLRVN
TLKATREKVLKALERSGAEPATIARHGVRIPAGEGASRLPNVTAEVSFQKGWFEVQDEGSQIVADLVFPREGEQVLDYCA
GGGGKTLAISAAMNNKGQVHAYDADRKRLAPIIERLKRAGTRNVQVHETAGSLASLVGRADRVLVDAPCTGTGTWRRRPD
TKWRLTQKNLEERLAQQEEALAGAARFVRPGGHLIYVTCSVLPEENESQVYGFCDDNPEFEVLSAADNWAALFGTDRPQP
WSADMKTVTLTPASTGTDGFFFCLMGRKN
>Mature_429_residues
MRLGGRLSGAIEVLDDIEKRKRPVADALRDWGLSHRFAGSGDRAAIGNIVYDALRMKLSHAYLMDSDSPVALAHAVLYRQ
WGFDVDTLAAEFADDRFAPEPPSEAMMTAFDQRRLEDAPLHVRADIPEWTQASFEENFSDDWLAEAQALAGRPTLDLRVN
TLKATREKVLKALERSGAEPATIARHGVRIPAGEGASRLPNVTAEVSFQKGWFEVQDEGSQIVADLVFPREGEQVLDYCA
GGGGKTLAISAAMNNKGQVHAYDADRKRLAPIIERLKRAGTRNVQVHETAGSLASLVGRADRVLVDAPCTGTGTWRRRPD
TKWRLTQKNLEERLAQQEEALAGAARFVRPGGHLIYVTCSVLPEENESQVYGFCDDNPEFEVLSAADNWAALFGTDRPQP
WSADMKTVTLTPASTGTDGFFFCLMGRKN

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=224, Percent_Identity=33.0357142857143, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI76150623, Length=224, Percent_Identity=33.0357142857143, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI32698918, Length=173, Percent_Identity=31.7919075144509, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI8922322, Length=293, Percent_Identity=27.3037542662116, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI270288818, Length=293, Percent_Identity=27.3037542662116, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI23199998, Length=293, Percent_Identity=27.3037542662116, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI270288816, Length=293, Percent_Identity=27.3037542662116, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI2367212, Length=306, Percent_Identity=30.718954248366, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87081985, Length=236, Percent_Identity=31.7796610169492, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17536757, Length=233, Percent_Identity=31.7596566523605, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71998419, Length=300, Percent_Identity=26.3333333333333, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=290, Percent_Identity=30.3448275862069, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319447, Length=212, Percent_Identity=27.3584905660377, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI22024126, Length=228, Percent_Identity=32.4561403508772, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI21356579, Length=224, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24668781, Length=257, Percent_Identity=27.6264591439689, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21356513, Length=257, Percent_Identity=27.6264591439689, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21355201, Length=284, Percent_Identity=29.2253521126761, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 47211; Mature: 47211

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLGGRLSGAIEVLDDIEKRKRPVADALRDWGLSHRFAGSGDRAAIGNIVYDALRMKLSH
CCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AYLMDSDSPVALAHAVLYRQWGFDVDTLAAEFADDRFAPEPPSEAMMTAFDQRRLEDAPL
HEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCE
HVRADIPEWTQASFEENFSDDWLAEAQALAGRPTLDLRVNTLKATREKVLKALERSGAEP
EEEECCCHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCC
ATIARHGVRIPAGEGASRLPNVTAEVSFQKGWFEVQDEGSQIVADLVFPREGEQVLDYCA
HHHHHCCEECCCCCCHHHCCCCEEEEEECCCCEEECCCCCEEEEECCCCCCCHHHHHHHC
GGGGKTLAISAAMNNKGQVHAYDADRKRLAPIIERLKRAGTRNVQVHETAGSLASLVGRA
CCCCCEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCC
DRVLVDAPCTGTGTWRRRPDTKWRLTQKNLEERLAQQEEALAGAARFVRPGGHLIYVTCS
CEEEEECCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
VLPEENESQVYGFCDDNPEFEVLSAADNWAALFGTDRPQPWSADMKTVTLTPASTGTDGF
ECCCCCCCCEEEEECCCCCEEEEECCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCE
FFCLMGRKN
EEEEECCCC
>Mature Secondary Structure
MRLGGRLSGAIEVLDDIEKRKRPVADALRDWGLSHRFAGSGDRAAIGNIVYDALRMKLSH
CCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AYLMDSDSPVALAHAVLYRQWGFDVDTLAAEFADDRFAPEPPSEAMMTAFDQRRLEDAPL
HEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCE
HVRADIPEWTQASFEENFSDDWLAEAQALAGRPTLDLRVNTLKATREKVLKALERSGAEP
EEEECCCHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCC
ATIARHGVRIPAGEGASRLPNVTAEVSFQKGWFEVQDEGSQIVADLVFPREGEQVLDYCA
HHHHHCCEECCCCCCHHHCCCCEEEEEECCCCEEECCCCCEEEEECCCCCCCHHHHHHHC
GGGGKTLAISAAMNNKGQVHAYDADRKRLAPIIERLKRAGTRNVQVHETAGSLASLVGRA
CCCCCEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCC
DRVLVDAPCTGTGTWRRRPDTKWRLTQKNLEERLAQQEEALAGAARFVRPGGHLIYVTCS
CEEEEECCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
VLPEENESQVYGFCDDNPEFEVLSAADNWAALFGTDRPQPWSADMKTVTLTPASTGTDGF
ECCCCCCCCEEEEECCCCCEEEEECCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCE
FFCLMGRKN
EEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA