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The map label for this gene is ilvE [H]

Identifier: 150398611

GI number: 150398611

Start: 3630346

End: 3631443

Strand: Reverse

Name: ilvE [H]

Synonym: Smed_3425

Alternate gene names: 150398611

Gene position: 3631443-3630346 (Counterclockwise)

Preceding gene: 150398612

Following gene: 150398609

Centisome position: 96.02

GC content: 63.48

Gene sequence:

>1098_bases
ATGACCGGTGCCGGTGAGCAGACTTTCCTGTTCGAGCAAAACGCGAACCCCTTGGCAACGAGCGAGCGCGAGGCTCTGCT
TGCTAATCCCGGCTTCGGGCGCGTGTTCACCGACCATATGGTGACGATCCGCTATTGCGAAAGCCGCGGCTGGCACGATG
CGAAGGTTGGCCCGAGAAAAGCCTTTGATCTCGATCCGTCCACACTCGTGCTGCACTACGCGCAGGAGATCTTCGAGGGC
ATGAAGGCCTATGGGCTGCCCGGCGGCGGCGCCACGCTGTTTCGTCCCGATGCGAATGCGCGGCGTTTCCGCAACTCCGC
ACTTCGGCTCGCAATGGCGCCCCTGCCGGAGGAGCTCTTCATCCGATCGGTACGCGAACTGGTGCGTGTCGACCGGGACT
GGATACCGGCCGCTGAAGGAGCCGCACTCTATCTCCGGCCGTTCATGATTGCCACGGAGGTACTGCTTGGGGTGAAGCCT
TCGGCCGAATACCTCTACTGCGTCATTGCTTCCTCTGTCGGTTCCTACTTCAAGGGTGGCGCTCCAGCGGTAACCATATG
GGTTTCGGAGAACTTCACGCGGGCAGCGCCCGGCGGCACCGGAGAGGCAAAATGCGGCGGCAACTATGCCGCAAGCCTCG
CGGCTCAGGCCGAGGCGATGCAGGAGGGCTGCGAACAGGTGGTGTTCCTGGACGCGGTGGAACGCCGCTTTGTCGAAGAA
CTCGGCGGCATGAACGTCTTCTTCGTCTTCCGGGACGGCTCATTGCAGACGCCTCCGCTCACCGGCACCATTCTCCCGGG
CATCACGCGCGATTCGCTGATCACGCTCGCCCGCGATATGGGGATGACGGTGCGTGAAGAACCCTATGCGATCGATCAGT
GGCAGGCGGATGCGGAAAGCGGCCGTCTCACCGAAGCCTTTGCCTGCGGGACGGCGGCTGTTGTGACCCCGATCGGCAAG
GTCAAGGGCCGCGAGCACAGCTTCACCATCGGTGACGGTGGTGCCGGTCCGGTCGCAAACCGCCTGAAGGCGGCACTGCT
CGATATCCAGAACGGCCGCGCGCCGGACAGGCATGGCTGGCTCGACCGGCTGTTCTAA

Upstream 100 bases:

>100_bases
AGGTCTTGTCTTCTCATCGTATAGGATGAAAGGAACGTCCAGCAGCAGAGATATATAATGCGGGCGCATCAATCGCTCGC
AACGAAGGAGCAGACGACAG

Downstream 100 bases:

>100_bases
CCGCCATAAGCGTGTCTGGTCAGCGGCCCCTCACATCAAGAGATAAGCCCATATCGACACGCTGACCACGCCGAGCGCGG
TCGTCAGCGTAATGGTCGAG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTGAGEQTFLFEQNANPLATSEREALLANPGFGRVFTDHMVTIRYCESRGWHDAKVGPRKAFDLDPSTLVLHYAQEIFEG
MKAYGLPGGGATLFRPDANARRFRNSALRLAMAPLPEELFIRSVRELVRVDRDWIPAAEGAALYLRPFMIATEVLLGVKP
SAEYLYCVIASSVGSYFKGGAPAVTIWVSENFTRAAPGGTGEAKCGGNYAASLAAQAEAMQEGCEQVVFLDAVERRFVEE
LGGMNVFFVFRDGSLQTPPLTGTILPGITRDSLITLARDMGMTVREEPYAIDQWQADAESGRLTEAFACGTAAVVTPIGK
VKGREHSFTIGDGGAGPVANRLKAALLDIQNGRAPDRHGWLDRLF

Sequences:

>Translated_365_residues
MTGAGEQTFLFEQNANPLATSEREALLANPGFGRVFTDHMVTIRYCESRGWHDAKVGPRKAFDLDPSTLVLHYAQEIFEG
MKAYGLPGGGATLFRPDANARRFRNSALRLAMAPLPEELFIRSVRELVRVDRDWIPAAEGAALYLRPFMIATEVLLGVKP
SAEYLYCVIASSVGSYFKGGAPAVTIWVSENFTRAAPGGTGEAKCGGNYAASLAAQAEAMQEGCEQVVFLDAVERRFVEE
LGGMNVFFVFRDGSLQTPPLTGTILPGITRDSLITLARDMGMTVREEPYAIDQWQADAESGRLTEAFACGTAAVVTPIGK
VKGREHSFTIGDGGAGPVANRLKAALLDIQNGRAPDRHGWLDRLF
>Mature_364_residues
TGAGEQTFLFEQNANPLATSEREALLANPGFGRVFTDHMVTIRYCESRGWHDAKVGPRKAFDLDPSTLVLHYAQEIFEGM
KAYGLPGGGATLFRPDANARRFRNSALRLAMAPLPEELFIRSVRELVRVDRDWIPAAEGAALYLRPFMIATEVLLGVKPS
AEYLYCVIASSVGSYFKGGAPAVTIWVSENFTRAAPGGTGEAKCGGNYAASLAAQAEAMQEGCEQVVFLDAVERRFVEEL
GGMNVFFVFRDGSLQTPPLTGTILPGITRDSLITLARDMGMTVREEPYAIDQWQADAESGRLTEAFACGTAAVVTPIGKV
KGREHSFTIGDGGAGPVANRLKAALLDIQNGRAPDRHGWLDRLF

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI38176287, Length=332, Percent_Identity=39.1566265060241, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI296010904, Length=332, Percent_Identity=39.1566265060241, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI296010906, Length=332, Percent_Identity=39.1566265060241, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI50658084, Length=338, Percent_Identity=37.2781065088757, Blast_Score=215, Evalue=5e-56,
Organism=Homo sapiens, GI296010902, Length=332, Percent_Identity=35.5421686746988, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI296010900, Length=332, Percent_Identity=35.5421686746988, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI258614015, Length=294, Percent_Identity=37.7551020408163, Blast_Score=180, Evalue=2e-45,
Organism=Escherichia coli, GI48994963, Length=321, Percent_Identity=33.9563862928349, Blast_Score=147, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI17568601, Length=296, Percent_Identity=39.527027027027, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17565728, Length=343, Percent_Identity=35.5685131195335, Blast_Score=191, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6322002, Length=354, Percent_Identity=39.5480225988701, Blast_Score=235, Evalue=9e-63,
Organism=Saccharomyces cerevisiae, GI6322608, Length=345, Percent_Identity=37.6811594202899, Blast_Score=221, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24641779, Length=338, Percent_Identity=38.4615384615385, Blast_Score=222, Evalue=3e-58,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 39584; Mature: 39453

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGAGEQTFLFEQNANPLATSEREALLANPGFGRVFTDHMVTIRYCESRGWHDAKVGPRK
CCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHEEEEEEECCCCCCCCCCCCCC
AFDLDPSTLVLHYAQEIFEGMKAYGLPGGGATLFRPDANARRFRNSALRLAMAPLPEELF
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHEEECCCCHHHH
IRSVRELVRVDRDWIPAAEGAALYLRPFMIATEVLLGVKPSAEYLYCVIASSVGSYFKGG
HHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCHHEEEEEEHHHHHHHHCCC
APAVTIWVSENFTRAAPGGTGEAKCGGNYAASLAAQAEAMQEGCEQVVFLDAVERRFVEE
CCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGMNVFFVFRDGSLQTPPLTGTILPGITRDSLITLARDMGMTVREEPYAIDQWQADAES
HCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GRLTEAFACGTAAVVTPIGKVKGREHSFTIGDGGAGPVANRLKAALLDIQNGRAPDRHGW
CCCHHHHHCCCHHHHCCHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCH
LDRLF
HHHCC
>Mature Secondary Structure 
TGAGEQTFLFEQNANPLATSEREALLANPGFGRVFTDHMVTIRYCESRGWHDAKVGPRK
CCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHEEEEEEECCCCCCCCCCCCCC
AFDLDPSTLVLHYAQEIFEGMKAYGLPGGGATLFRPDANARRFRNSALRLAMAPLPEELF
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHEEECCCCHHHH
IRSVRELVRVDRDWIPAAEGAALYLRPFMIATEVLLGVKPSAEYLYCVIASSVGSYFKGG
HHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCHHEEEEEEHHHHHHHHCCC
APAVTIWVSENFTRAAPGGTGEAKCGGNYAASLAAQAEAMQEGCEQVVFLDAVERRFVEE
CCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGMNVFFVFRDGSLQTPPLTGTILPGITRDSLITLARDMGMTVREEPYAIDQWQADAES
HCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GRLTEAFACGTAAVVTPIGKVKGREHSFTIGDGGAGPVANRLKAALLDIQNGRAPDRHGW
CCCHHHHHCCCHHHHCCHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCH
LDRLF
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]