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The map label for this gene is ghrA [H]

Identifier: 150398551

GI number: 150398551

Start: 3561585

End: 3562544

Strand: Reverse

Name: ghrA [H]

Synonym: Smed_3362

Alternate gene names: 150398551

Gene position: 3562544-3561585 (Counterclockwise)

Preceding gene: 150398552

Following gene: 150398547

Centisome position: 94.2

GC content: 64.06

Gene sequence:

>960_bases
ATGTCCGCCAAAAGCCCGGTGATCGTCGACCTGAAATTCATCCCCGAAGAGGTGGAGGCGGCCCTTGCAGGGGCCTTTCC
CGACCGAGAGGTCATCGATCTCGCTGACCCGGTGCATCGGGGGCGTGACCTGTCCGGCATCGATTATGCCGTGGTCTGGA
AATCCGCCCCCGACCTCTTTTCGCGGGCACCGGACCTCAAAGTGGTCTTTTCCGGCGGCGCCGGCGTCGACCACGTGCTG
ACGCTTCCCGGCCTGCCCGACGTGCCGCTGGTGCGCTTCGTCGATCGGACGTTGACGACGCGGATGAGCGAGTGGGTCGT
AATGCAGTGCCTCCTGCATCTTCGCCAGCACCACGCCTACGAGGCGCTTGCGAACAGGCACGAATGGCGCGACCTCAGTC
AGCCAGAGGCAGCCGACGTGACCGTCGGCATCATGGGAATGGGCGTGCTCGGCCAGGACGCCGCCCGCAAGCTCGCCGTC
ATGGGCTTCAAGGTGATCGGCTGGTCGCGAAGCAAGCGGGTGATCGAGGGTGTAGAGACCTACGACGCTGCCGGACTGGA
CGCATTTCTCGGCCAAACGGATTTCCTCGTCGGCCTGCTGCCGCTGACGCCGGAGACCAGAGGCATATTCAACGGCGCCC
TTTTTGCCAAGCTCAGCCGCCGAGGACCTTTCGGCGCGCCTGTTTTCATCAATGCAGGCCGGGGCGGCAGCCAGGTCGAG
GCCGATATCCTGGAATGCATCGATTCCAGTATGCTCGCAGGCGCCTCGCTCGACGTCTTCGAACGGGAGCCGCTCCCGCA
GGAAAGCCGCTTCTGGGACCTGCCGAACGTCTACGTGACCCCGCATGTCGCAGCCTCGTCCGACGTCAGGGCCCTCTTCG
TCCATGTAGAACATCAGATCGCGCGCTTCGAGAGCGGGCTTCCCCTGGAACATGTGGTGGATAAGGTAGCCGGCTACTGA

Upstream 100 bases:

>100_bases
TCGATGCGCCCGCGGAAGACTATACACGGGCGCTGATGGCCGCCGCCTTCAATCTCGAAGCGGTCAATCTCGCCGCCGTT
CATCAGTAGAGTTTTCGTCC

Downstream 100 bases:

>100_bases
TTGGCCGGTGTCCGGTCGCGCTCCCCCGCGTACGGGGAGAGGGCAAGGGTTGAAGGGGCAAATCTGCGGGCGATCATGCC
GGCAATAAAGCCTGACCGTT

Product: NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSAKSPVIVDLKFIPEEVEAALAGAFPDREVIDLADPVHRGRDLSGIDYAVVWKSAPDLFSRAPDLKVVFSGGAGVDHVL
TLPGLPDVPLVRFVDRTLTTRMSEWVVMQCLLHLRQHHAYEALANRHEWRDLSQPEAADVTVGIMGMGVLGQDAARKLAV
MGFKVIGWSRSKRVIEGVETYDAAGLDAFLGQTDFLVGLLPLTPETRGIFNGALFAKLSRRGPFGAPVFINAGRGGSQVE
ADILECIDSSMLAGASLDVFEREPLPQESRFWDLPNVYVTPHVAASSDVRALFVHVEHQIARFESGLPLEHVVDKVAGY

Sequences:

>Translated_319_residues
MSAKSPVIVDLKFIPEEVEAALAGAFPDREVIDLADPVHRGRDLSGIDYAVVWKSAPDLFSRAPDLKVVFSGGAGVDHVL
TLPGLPDVPLVRFVDRTLTTRMSEWVVMQCLLHLRQHHAYEALANRHEWRDLSQPEAADVTVGIMGMGVLGQDAARKLAV
MGFKVIGWSRSKRVIEGVETYDAAGLDAFLGQTDFLVGLLPLTPETRGIFNGALFAKLSRRGPFGAPVFINAGRGGSQVE
ADILECIDSSMLAGASLDVFEREPLPQESRFWDLPNVYVTPHVAASSDVRALFVHVEHQIARFESGLPLEHVVDKVAGY
>Mature_318_residues
SAKSPVIVDLKFIPEEVEAALAGAFPDREVIDLADPVHRGRDLSGIDYAVVWKSAPDLFSRAPDLKVVFSGGAGVDHVLT
LPGLPDVPLVRFVDRTLTTRMSEWVVMQCLLHLRQHHAYEALANRHEWRDLSQPEAADVTVGIMGMGVLGQDAARKLAVM
GFKVIGWSRSKRVIEGVETYDAAGLDAFLGQTDFLVGLLPLTPETRGIFNGALFAKLSRRGPFGAPVFINAGRGGSQVEA
DILECIDSSMLAGASLDVFEREPLPQESRFWDLPNVYVTPHVAASSDVRALFVHVEHQIARFESGLPLEHVVDKVAGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=252, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI6912396, Length=231, Percent_Identity=29.8701298701299, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI87081824, Length=284, Percent_Identity=39.4366197183099, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI87082289, Length=150, Percent_Identity=34.6666666666667, Blast_Score=85, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17532191, Length=248, Percent_Identity=27.8225806451613, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322116, Length=160, Percent_Identity=28.75, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320925, Length=160, Percent_Identity=28.75, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28574286, Length=257, Percent_Identity=29.1828793774319, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24585514, Length=298, Percent_Identity=26.510067114094, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28574282, Length=298, Percent_Identity=26.510067114094, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45551003, Length=298, Percent_Identity=26.8456375838926, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28574284, Length=298, Percent_Identity=26.510067114094, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45552429, Length=298, Percent_Identity=26.8456375838926, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585516, Length=179, Percent_Identity=29.608938547486, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28571528, Length=147, Percent_Identity=31.2925170068027, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34802; Mature: 34671

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKSPVIVDLKFIPEEVEAALAGAFPDREVIDLADPVHRGRDLSGIDYAVVWKSAPDLF
CCCCCCEEEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHH
SRAPDLKVVFSGGAGVDHVLTLPGLPDVPLVRFVDRTLTTRMSEWVVMQCLLHLRQHHAY
HCCCCEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALANRHEWRDLSQPEAADVTVGIMGMGVLGQDAARKLAVMGFKVIGWSRSKRVIEGVET
HHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHEEEECCCCCHHHHHHHHH
YDAAGLDAFLGQTDFLVGLLPLTPETRGIFNGALFAKLSRRGPFGAPVFINAGRGGSQVE
HCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHH
ADILECIDSSMLAGASLDVFEREPLPQESRFWDLPNVYVTPHVAASSDVRALFVHVEHQI
HHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEHHHHHHH
ARFESGLPLEHVVDKVAGY
HHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SAKSPVIVDLKFIPEEVEAALAGAFPDREVIDLADPVHRGRDLSGIDYAVVWKSAPDLF
CCCCCEEEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHH
SRAPDLKVVFSGGAGVDHVLTLPGLPDVPLVRFVDRTLTTRMSEWVVMQCLLHLRQHHAY
HCCCCEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALANRHEWRDLSQPEAADVTVGIMGMGVLGQDAARKLAVMGFKVIGWSRSKRVIEGVET
HHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHEEEECCCCCHHHHHHHHH
YDAAGLDAFLGQTDFLVGLLPLTPETRGIFNGALFAKLSRRGPFGAPVFINAGRGGSQVE
HCCCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHH
ADILECIDSSMLAGASLDVFEREPLPQESRFWDLPNVYVTPHVAASSDVRALFVHVEHQI
HHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEHHHHHHH
ARFESGLPLEHVVDKVAGY
HHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA