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The map label for this gene is proP [H]

Identifier: 150398372

GI number: 150398372

Start: 3345216

End: 3347105

Strand: Reverse

Name: proP [H]

Synonym: Smed_3180

Alternate gene names: 150398372

Gene position: 3347105-3345216 (Counterclockwise)

Preceding gene: 150398377

Following gene: 150398369

Centisome position: 88.5

GC content: 61.53

Gene sequence:

>1890_bases
ATGGCAAACGTCGTGACAGCGGGCGGCACAAAAGCCGGCCCGATGACCGGCGAGGAGAAGAAGGTCATCTTCGCCTCGTC
GCTCGGTACCGTCTTCGAATGGTACGATTTCTATCTTTACGGCTCACTTGCCGTTTATATCGGTGCAACCTTCTTCAGCC
AGTATCCGGAGACAACGCGCAACATCTTCGCGCTGCTCGCCTTCGCCGCCGGCTTCCTGGTCCGGCCGTTCGGCGCACTG
GTTTTCGGCCGCCTGGGCGATCTCGTCGGGCGCAAATACACGTTCCTCGTCACGATCCTGATCATGGGTCTGTCGACATT
CCTCGTCGGCGTTCTGCCCGGTGCGGCCTCGATCGGCATCGCTGCGCCGATCATCCTGATCGCGCTCCGCCTCCTGCAGG
GCCTCGCGCTTGGCGGTGAATACGGCGGTGCTGCAACCTATGTTGCAGAGCATGCGCCGCATGGCCGGCGCGGTTATTTC
ACGTCGTGGATTCAGACGACGGCGACGCTCGGTCTCTTCCTCTCGCTGGTCGTTATTCTTCTGGTCCAATATATGCTTGG
CAAGGAAGCCTTCGCCGAGTGGGGCTGGCGCATACCGTTCCTCCTATCCTTCGTGCTTCTTGGCGTTTCCGTCTGGATCC
GCCTGAAGATGAACGAATCGCCTGCCTTCAAGAAGATGAAGGAGGAAGGCAAGGGTTCGAAGGCCCCGCTGACGGAAGCA
TTCGGCCATTGGCGCAACGCCAAGATCGCCCTTCTGGCTCTCTTCGGCGCTGTCGTCGGCCAGGCCGTGGTCTGGTATTC
CGGCCAATTCTACGCGCTGTTCTTCCTGCAGAGCATACTAAAGGTCGACGGCCAGTCGGCAAACCTGATGGTCGCCGCCT
CGCTCCTGCTCGGCACGGGCTTTTTCGTCTTCTTCGGCTGGCTGTCGGACAAGATCGGCCGTAAGCCGATCATCATGGCG
GGCCTTCTGCTTGCCATGCTGACCTACTTCCCGCTGTTCAAAGCGCTGACCTGGGCCGGCAATCCCGCACTTGCGGAAGC
GCAGCAGAGCGTCCGCGCAACCGTTACCGCGGCTCCGGGCGACTGCAAGTTCCAGTTCAACCCGACCGGTACGGCGAAGT
TCACCACGTCGTGCGATATAGCGACCGCGTTCCTGACCAGGAACTCCGTTCCCTACGACGTCGTGACCTCGGCGGCTGCA
GGGACGCCGGCGACGGTGAAGATCGGCGAAACGACGATCACCAGCTATGACGCGATCGCGGCCGGCGACAAGGCAAAGGC
CGAGGAAGCGGCCTTTGGCAAACAGATCAACATGGCACTGCAGTCCTCGGGCTATCCGCTGGTGCGCGGAGCCGCCAAGG
TGCCGGAATCGAAGCTCGACGCTTTCGTCGCGGCTAATCCGGAGCTTGCACTCGACGCGGCTGCCGTACGCGCCGGCGAA
AAGACCATGGTCCCGGCCGACAAGCTCGTTGCAGACAAGCTGCTCACGCAGGAAGAGGTCGGCAGCGCCACCGAAATGGC
CGTCTATTCTATAGACAAGGGCGGCGCCTTCACCATGGTGGCCGACCCGGCTCGCGTCAACTGGACGGTGATCATCGCCG
TGCTGACCGTGCTCGTCATCTATGTGACCATGGTCTACGGCCCAATCGCGGCACTGCTCGTCGAGCTCTTCCCGACACGC
ATCCGTTACACGGGCATGTCGCTGCCTTACCATATCGGCAATGGCTGGTTCGGTGGGCTGCTTCCGGCAACGGCCTTCGC
GATGAGCGCGGCCAAGGGCGATATCTACTACGGCCTGTGGTATCCGATCGTATTTGCGGGCATCACGCTGGTCATCGGCC
TTCTGTTCCTGCCCGAAACGAAGGACCGGGATATCCACACGATGGAGTGA

Upstream 100 bases:

>100_bases
CCATGCCCGGGCGTCAAAGGGGACCCCGACGTTCAGGGAGGAATGTCCGGACCGGGAGATGGGCGCTCCAATATATATTT
TGGAGTGGAGGAATATCATA

Downstream 100 bases:

>100_bases
GCCGCTTGGCTACTCCACATATCTCCTGACTCCGGCGCGGCTTCCGCGCCGGCTTTTTATTTGCTCTCGGGCGTCGCATG
CCGCAGCACCGGCCAGCCGT

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MANVVTAGGTKAGPMTGEEKKVIFASSLGTVFEWYDFYLYGSLAVYIGATFFSQYPETTRNIFALLAFAAGFLVRPFGAL
VFGRLGDLVGRKYTFLVTILIMGLSTFLVGVLPGAASIGIAAPIILIALRLLQGLALGGEYGGAATYVAEHAPHGRRGYF
TSWIQTTATLGLFLSLVVILLVQYMLGKEAFAEWGWRIPFLLSFVLLGVSVWIRLKMNESPAFKKMKEEGKGSKAPLTEA
FGHWRNAKIALLALFGAVVGQAVVWYSGQFYALFFLQSILKVDGQSANLMVAASLLLGTGFFVFFGWLSDKIGRKPIIMA
GLLLAMLTYFPLFKALTWAGNPALAEAQQSVRATVTAAPGDCKFQFNPTGTAKFTTSCDIATAFLTRNSVPYDVVTSAAA
GTPATVKIGETTITSYDAIAAGDKAKAEEAAFGKQINMALQSSGYPLVRGAAKVPESKLDAFVAANPELALDAAAVRAGE
KTMVPADKLVADKLLTQEEVGSATEMAVYSIDKGGAFTMVADPARVNWTVIIAVLTVLVIYVTMVYGPIAALLVELFPTR
IRYTGMSLPYHIGNGWFGGLLPATAFAMSAAKGDIYYGLWYPIVFAGITLVIGLLFLPETKDRDIHTME

Sequences:

>Translated_629_residues
MANVVTAGGTKAGPMTGEEKKVIFASSLGTVFEWYDFYLYGSLAVYIGATFFSQYPETTRNIFALLAFAAGFLVRPFGAL
VFGRLGDLVGRKYTFLVTILIMGLSTFLVGVLPGAASIGIAAPIILIALRLLQGLALGGEYGGAATYVAEHAPHGRRGYF
TSWIQTTATLGLFLSLVVILLVQYMLGKEAFAEWGWRIPFLLSFVLLGVSVWIRLKMNESPAFKKMKEEGKGSKAPLTEA
FGHWRNAKIALLALFGAVVGQAVVWYSGQFYALFFLQSILKVDGQSANLMVAASLLLGTGFFVFFGWLSDKIGRKPIIMA
GLLLAMLTYFPLFKALTWAGNPALAEAQQSVRATVTAAPGDCKFQFNPTGTAKFTTSCDIATAFLTRNSVPYDVVTSAAA
GTPATVKIGETTITSYDAIAAGDKAKAEEAAFGKQINMALQSSGYPLVRGAAKVPESKLDAFVAANPELALDAAAVRAGE
KTMVPADKLVADKLLTQEEVGSATEMAVYSIDKGGAFTMVADPARVNWTVIIAVLTVLVIYVTMVYGPIAALLVELFPTR
IRYTGMSLPYHIGNGWFGGLLPATAFAMSAAKGDIYYGLWYPIVFAGITLVIGLLFLPETKDRDIHTME
>Mature_628_residues
ANVVTAGGTKAGPMTGEEKKVIFASSLGTVFEWYDFYLYGSLAVYIGATFFSQYPETTRNIFALLAFAAGFLVRPFGALV
FGRLGDLVGRKYTFLVTILIMGLSTFLVGVLPGAASIGIAAPIILIALRLLQGLALGGEYGGAATYVAEHAPHGRRGYFT
SWIQTTATLGLFLSLVVILLVQYMLGKEAFAEWGWRIPFLLSFVLLGVSVWIRLKMNESPAFKKMKEEGKGSKAPLTEAF
GHWRNAKIALLALFGAVVGQAVVWYSGQFYALFFLQSILKVDGQSANLMVAASLLLGTGFFVFFGWLSDKIGRKPIIMAG
LLLAMLTYFPLFKALTWAGNPALAEAQQSVRATVTAAPGDCKFQFNPTGTAKFTTSCDIATAFLTRNSVPYDVVTSAAAG
TPATVKIGETTITSYDAIAAGDKAKAEEAAFGKQINMALQSSGYPLVRGAAKVPESKLDAFVAANPELALDAAAVRAGEK
TMVPADKLVADKLLTQEEVGSATEMAVYSIDKGGAFTMVADPARVNWTVIIAVLTVLVIYVTMVYGPIAALLVELFPTRI
RYTGMSLPYHIGNGWFGGLLPATAFAMSAAKGDIYYGLWYPIVFAGITLVIGLLFLPETKDRDIHTME

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=328, Percent_Identity=35.0609756097561, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1788292, Length=331, Percent_Identity=34.441087613293, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1788942, Length=348, Percent_Identity=33.6206896551724, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1789941, Length=337, Percent_Identity=35.9050445103858, Blast_Score=155, Evalue=9e-39,
Organism=Escherichia coli, GI87082404, Length=325, Percent_Identity=26.4615384615385, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI87082231, Length=229, Percent_Identity=26.6375545851528, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 67412; Mature: 67280

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS00107 PROTEIN_KINASE_ATP ; PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVTAGGTKAGPMTGEEKKVIFASSLGTVFEWYDFYLYGSLAVYIGATFFSQYPETTR
CCCEEECCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
NIFALLAFAAGFLVRPFGALVFGRLGDLVGRKYTFLVTILIMGLSTFLVGVLPGAASIGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
AAPIILIALRLLQGLALGGEYGGAATYVAEHAPHGRRGYFTSWIQTTATLGLFLSLVVIL
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LVQYMLGKEAFAEWGWRIPFLLSFVLLGVSVWIRLKMNESPAFKKMKEEGKGSKAPLTEA
HHHHHHCHHHHHHCCCCHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHCCCCCCCHHHH
FGHWRNAKIALLALFGAVVGQAVVWYSGQFYALFFLQSILKVDGQSANLMVAASLLLGTG
HHCCCCCHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
FFVFFGWLSDKIGRKPIIMAGLLLAMLTYFPLFKALTWAGNPALAEAQQSVRATVTAAPG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEECCC
DCKFQFNPTGTAKFTTSCDIATAFLTRNSVPYDVVTSAAAGTPATVKIGETTITSYDAIA
CEEEEECCCCCEEEECCCHHHHHHHHCCCCCHHHHHHCCCCCCCEEEECCEEECCCHHHC
AGDKAKAEEAAFGKQINMALQSSGYPLVRGAAKVPESKLDAFVAANPELALDAAAVRAGE
CCCCCHHHHHHHHHHHHHHHHCCCCCEECCHHHCCHHHHCEEEECCCCHHHHHHHHHCCC
KTMVPADKLVADKLLTQEEVGSATEMAVYSIDKGGAFTMVADPARVNWTVIIAVLTVLVI
CCCCCHHHHHHHHHHHHHHHCCCHHEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHH
YVTMVYGPIAALLVELFPTRIRYTGMSLPYHIGNGWFGGLLPATAFAMSAAKGDIYYGLW
HHHHHHHHHHHHHHHHHHHHHEEECCCCCEECCCCCCHHHHHHHHHHHHHCCCCEEEHHH
YPIVFAGITLVIGLLFLPETKDRDIHTME
HHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
ANVVTAGGTKAGPMTGEEKKVIFASSLGTVFEWYDFYLYGSLAVYIGATFFSQYPETTR
CCEEECCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH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HHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]