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The map label for this gene is paaF [C]

Identifier: 150398364

GI number: 150398364

Start: 3334795

End: 3335574

Strand: Reverse

Name: paaF [C]

Synonym: Smed_3172

Alternate gene names: 150398364

Gene position: 3335574-3334795 (Counterclockwise)

Preceding gene: 150398365

Following gene: 150398363

Centisome position: 88.2

GC content: 65.13

Gene sequence:

>780_bases
ATGGCTGACGAACGAATCCGTCTCGATTTCGACGGTCCGATCGCGACCCTGACGGTTTCGCGGCCGGAAAAGCTCAATGC
CTTCGACATCGACATGCTGAGAGCGCTCAGCGCAGCCTGCGATACCGTGGAGGCGAAACGCGACGTGCGCGTTATCATCC
TTACCGGCGAGGGCAAGGCCTTTTCCGCCGGTGGCGACATCAAGGCCTGGGGCGGCATGGAGCCAGCCTCCTTCGGCCAT
GACTGGGTTCGGTTCGGTCACCGTGTTTTCGAGCGGCTCGCCACGCTGCGCATGCCGGTCGTCGCTGCGCTCAACGGCCA
CGCGCTCGGCGGCGGTCTGGAACTGGCGGCGGCGGCGGATATTCGCCTGGCCGAAAAACATGTGAAGATCGGCCTGCCGG
AGACGAGCCTCGGCATGGTTCCCGGCTGGTCCGGTACGCAACGCCTGGTGGCCCGGTTCGGCGCGCAGATCGTCCGCCGC
ATGGTGCTTGGCGGCGAGATGTTCACGGCGGCACAAGCCGAGGCCGAAGGGCTGGTCGACGCCGTCGTCGAGACCGGCAC
GGTCATGCACGCAGCGCTTGATTATGGCAGGCGGATCGCCAGCCGCGGCCCCGCGGCACTGGAAATATCGAAACTGATGA
TCGCCTCGGCAAGCGGCGAGGACAAGGGCGCGGCGGTGGAAGCGCTGGGTTCGATTCTCGTCGCCAAGACCGGCGATCTC
AAGGAAGGCGTGGCGGCTTTCAGTGAAAAGCGCGAAGCATGCTTCAAGGGAGAATGGTGA

Upstream 100 bases:

>100_bases
TCCGACAAACTCAAGCTCATGGATTCGGCACTCTTCGGTGAAGCGCCTATCGGGCTGGCGCTGCCCATGAAAAGCCAGCG
CCGGCTGGCGGGAGGTGTCG

Downstream 100 bases:

>100_bases
TGACGGTACTCGTAAGGCCGAAGGCGCTCGGCGAATACAAGGTCCGTGAATTCCGAATGCTGATCGACGGCCAGTGGGTC
GATAGCGCCGAGGGCCGGAC

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MADERIRLDFDGPIATLTVSRPEKLNAFDIDMLRALSAACDTVEAKRDVRVIILTGEGKAFSAGGDIKAWGGMEPASFGH
DWVRFGHRVFERLATLRMPVVAALNGHALGGGLELAAAADIRLAEKHVKIGLPETSLGMVPGWSGTQRLVARFGAQIVRR
MVLGGEMFTAAQAEAEGLVDAVVETGTVMHAALDYGRRIASRGPAALEISKLMIASASGEDKGAAVEALGSILVAKTGDL
KEGVAAFSEKREACFKGEW

Sequences:

>Translated_259_residues
MADERIRLDFDGPIATLTVSRPEKLNAFDIDMLRALSAACDTVEAKRDVRVIILTGEGKAFSAGGDIKAWGGMEPASFGH
DWVRFGHRVFERLATLRMPVVAALNGHALGGGLELAAAADIRLAEKHVKIGLPETSLGMVPGWSGTQRLVARFGAQIVRR
MVLGGEMFTAAQAEAEGLVDAVVETGTVMHAALDYGRRIASRGPAALEISKLMIASASGEDKGAAVEALGSILVAKTGDL
KEGVAAFSEKREACFKGEW
>Mature_258_residues
ADERIRLDFDGPIATLTVSRPEKLNAFDIDMLRALSAACDTVEAKRDVRVIILTGEGKAFSAGGDIKAWGGMEPASFGHD
WVRFGHRVFERLATLRMPVVAALNGHALGGGLELAAAADIRLAEKHVKIGLPETSLGMVPGWSGTQRLVARFGAQIVRRM
VLGGEMFTAAQAEAEGLVDAVVETGTVMHAALDYGRRIASRGPAALEISKLMIASASGEDKGAAVEALGSILVAKTGDLK
EGVAAFSEKREACFKGEW

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=258, Percent_Identity=35.6589147286822, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI4502327, Length=252, Percent_Identity=34.9206349206349, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI68989263, Length=201, Percent_Identity=30.3482587064677, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI31542718, Length=254, Percent_Identity=25.9842519685039, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI20127408, Length=183, Percent_Identity=31.6939890710383, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI213417737, Length=175, Percent_Identity=29.1428571428571, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI157694516, Length=175, Percent_Identity=29.1428571428571, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI70995211, Length=250, Percent_Identity=25.2, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI37594469, Length=147, Percent_Identity=29.9319727891156, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI37594471, Length=147, Percent_Identity=29.9319727891156, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI62530384, Length=183, Percent_Identity=28.4153005464481, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI157694520, Length=134, Percent_Identity=29.8507462686567, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787659, Length=243, Percent_Identity=34.5679012345679, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1790281, Length=242, Percent_Identity=32.2314049586777, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI221142681, Length=265, Percent_Identity=33.2075471698113, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788682, Length=198, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787660, Length=251, Percent_Identity=32.2709163346614, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788597, Length=259, Percent_Identity=28.957528957529, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI87082183, Length=258, Percent_Identity=26.7441860465116, Blast_Score=78, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI25145438, Length=241, Percent_Identity=34.4398340248963, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17554946, Length=268, Percent_Identity=31.7164179104478, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=33.1818181818182, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17558304, Length=239, Percent_Identity=28.0334728033473, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17508951, Length=236, Percent_Identity=29.6610169491525, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17508953, Length=237, Percent_Identity=29.535864978903, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=237, Percent_Identity=29.535864978903, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=260, Percent_Identity=23.8461538461538, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=209, Percent_Identity=25.8373205741627, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25144160, Length=193, Percent_Identity=27.979274611399, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=193, Percent_Identity=27.979274611399, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=201, Percent_Identity=26.3681592039801, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=238, Percent_Identity=27.3109243697479, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25141363, Length=212, Percent_Identity=23.1132075471698, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17549921, Length=215, Percent_Identity=24.1860465116279, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20129971, Length=252, Percent_Identity=34.1269841269841, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24653477, Length=252, Percent_Identity=34.1269841269841, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=33.7301587301587, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922422, Length=280, Percent_Identity=25.7142857142857, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19920382, Length=206, Percent_Identity=28.1553398058252, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24583165, Length=168, Percent_Identity=30.952380952381, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19921018, Length=172, Percent_Identity=30.8139534883721, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357171, Length=218, Percent_Identity=28.4403669724771, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24650670, Length=242, Percent_Identity=27.6859504132231, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI28571730, Length=172, Percent_Identity=24.4186046511628, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28571729, Length=172, Percent_Identity=24.4186046511628, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27415; Mature: 27284

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADERIRLDFDGPIATLTVSRPEKLNAFDIDMLRALSAACDTVEAKRDVRVIILTGEGKA
CCCCEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCE
FSAGGDIKAWGGMEPASFGHDWVRFGHRVFERLATLRMPVVAALNGHALGGGLELAAAAD
EECCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHH
IRLAEKHVKIGLPETSLGMVPGWSGTQRLVARFGAQIVRRMVLGGEMFTAAQAEAEGLVD
HHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AVVETGTVMHAALDYGRRIASRGPAALEISKLMIASASGEDKGAAVEALGSILVAKTGDL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHEECCCCCCCHHHHHHHHHHHEECCCCH
KEGVAAFSEKREACFKGEW
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ADERIRLDFDGPIATLTVSRPEKLNAFDIDMLRALSAACDTVEAKRDVRVIILTGEGKA
CCCEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCE
FSAGGDIKAWGGMEPASFGHDWVRFGHRVFERLATLRMPVVAALNGHALGGGLELAAAAD
EECCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHH
IRLAEKHVKIGLPETSLGMVPGWSGTQRLVARFGAQIVRRMVLGGEMFTAAQAEAEGLVD
HHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AVVETGTVMHAALDYGRRIASRGPAALEISKLMIASASGEDKGAAVEALGSILVAKTGDL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHEECCCCCCCHHHHHHHHHHHEECCCCH
KEGVAAFSEKREACFKGEW
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA