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The map label for this gene is rhtB [H]

Identifier: 150398258

GI number: 150398258

Start: 3212963

End: 3213604

Strand: Direct

Name: rhtB [H]

Synonym: Smed_3064

Alternate gene names: 150398258

Gene position: 3212963-3213604 (Clockwise)

Preceding gene: 150398256

Following gene: 150398268

Centisome position: 84.96

GC content: 61.68

Gene sequence:

>642_bases
ATGGACTTCGTTCCCAGTCTGCCGACCTTGCTTGCCTTCGCTGCGGCGAGCCTGCTTCTCGCCGCAACGCCCGGACCCGA
CATGACGCTGTCGATCAGCCGGGCGCTGGCGCAGGGAAAGAAAGCAGCGCTCCTGGTCGTGCTCGGAACAGGCCTCGGCA
TCGTGGTCCACACGCTCCTCGTCGCCTTCGGCATCTCCGCTCTGATCACCGCATCCCCGACCGCCTTCCTGGCGCTGAAG
ACGGGGGGCGCCGCCTATCTTCTATGGCTGGCGATCCAGGCAATCCGCTATGGATCGAGCCTTTCGGTGACGAAGATGAC
GGATGCGAAGGGAAGCGGGTTTTCGAATGTCGCGACCGGATTCTCGGTCAACATTCTGAACCCGAAGGTGGTGATCTTCT
TCATGACGTTCCTGCCGCAGTTCGTCAGGGTCGATGACCCGGCGGTAACCGGCAAGCTCTTGTTTCTCGGCTTTTTCTTC
ATCGTCATCGGCATGCCGGTGAACGCGCTGGTCGTTCTTGCTGCGGACTGGCTCGCAGCCTGGCTGCAGCGCAACAGGCG
CGTGATGCGGGCGATCGACTACGGCTTCGCCGGCGTCTTCTCCATATTCGCGATCAAGATCTTCTTCACACAGGCGCGCT
GA

Upstream 100 bases:

>100_bases
GACGGGATACAAGCGTTAGCAGGCCGGGATCGCGCAAATCCGCTGGCAAAAGAGCCGCACCGCCGGTATCAGCTAAGACC
ATCTTTTCAAGGAATGCGCC

Downstream 100 bases:

>100_bases
GACGGCTTCGTTTGCATTTGCCTCTCATCTGCCTCCCGGCAGGCAGATGAGAGGCACGTCCCTGAAGGCGCGTCACCCGA
TCAACAGAGCATCATCGTCC

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MDFVPSLPTLLAFAAASLLLAATPGPDMTLSISRALAQGKKAALLVVLGTGLGIVVHTLLVAFGISALITASPTAFLALK
TGGAAYLLWLAIQAIRYGSSLSVTKMTDAKGSGFSNVATGFSVNILNPKVVIFFMTFLPQFVRVDDPAVTGKLLFLGFFF
IVIGMPVNALVVLAADWLAAWLQRNRRVMRAIDYGFAGVFSIFAIKIFFTQAR

Sequences:

>Translated_213_residues
MDFVPSLPTLLAFAAASLLLAATPGPDMTLSISRALAQGKKAALLVVLGTGLGIVVHTLLVAFGISALITASPTAFLALK
TGGAAYLLWLAIQAIRYGSSLSVTKMTDAKGSGFSNVATGFSVNILNPKVVIFFMTFLPQFVRVDDPAVTGKLLFLGFFF
IVIGMPVNALVVLAADWLAAWLQRNRRVMRAIDYGFAGVFSIFAIKIFFTQAR
>Mature_213_residues
MDFVPSLPTLLAFAAASLLLAATPGPDMTLSISRALAQGKKAALLVVLGTGLGIVVHTLLVAFGISALITASPTAFLALK
TGGAAYLLWLAIQAIRYGSSLSVTKMTDAKGSGFSNVATGFSVNILNPKVVIFFMTFLPQFVRVDDPAVTGKLLFLGFFF
IVIGMPVNALVVLAADWLAAWLQRNRRVMRAIDYGFAGVFSIFAIKIFFTQAR

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=197, Percent_Identity=27.9187817258883, Blast_Score=78, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22635; Mature: 22635

Theoretical pI: Translated: 11.03; Mature: 11.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFVPSLPTLLAFAAASLLLAATPGPDMTLSISRALAQGKKAALLVVLGTGLGIVVHTLL
CCCCCCHHHHHHHHHHHHHEEECCCCCEEHHHHHHHHCCCCEEEEEEECCCHHHHHHHHH
VAFGISALITASPTAFLALKTGGAAYLLWLAIQAIRYGSSLSVTKMTDAKGSGFSNVATG
HHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHCC
FSVNILNPKVVIFFMTFLPQFVRVDDPAVTGKLLFLGFFFIVIGMPVNALVVLAADWLAA
CEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
WLQRNRRVMRAIDYGFAGVFSIFAIKIFFTQAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
>Mature Secondary Structure
MDFVPSLPTLLAFAAASLLLAATPGPDMTLSISRALAQGKKAALLVVLGTGLGIVVHTLL
CCCCCCHHHHHHHHHHHHHEEECCCCCEEHHHHHHHHCCCCEEEEEEECCCHHHHHHHHH
VAFGISALITASPTAFLALKTGGAAYLLWLAIQAIRYGSSLSVTKMTDAKGSGFSNVATG
HHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHCC
FSVNILNPKVVIFFMTFLPQFVRVDDPAVTGKLLFLGFFFIVIGMPVNALVVLAADWLAA
CEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
WLQRNRRVMRAIDYGFAGVFSIFAIKIFFTQAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]