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The map label for this gene is imuB [H]

Identifier: 150398217

GI number: 150398217

Start: 3172430

End: 3173845

Strand: Reverse

Name: imuB [H]

Synonym: Smed_3022

Alternate gene names: 150398217

Gene position: 3173845-3172430 (Counterclockwise)

Preceding gene: 150398218

Following gene: 150398216

Centisome position: 83.92

GC content: 66.03

Gene sequence:

>1416_bases
ATGCGGCTCGTCGCGCTCGATACTGTTGCGGAGCGTGTCGGCCTGAAGCGTGGCCAGGGGGCGGCGGAAGCCCGCGCCAT
GTGTCCCTCTCTCGACGTGATCGCCTTCGATCCGGCCGCCGATCAGGCCTTTCTGGAGGCGCTTGCCGACTGGTGCGACC
GCTATACGCCGCTCGTGGCACTCGACGGCAAGGACGGCCTGTTCCTCGACATCACCGGCTGCGCCCATCTTCATGGCGGC
GAGAAGGCGCTCGTCAGCGATGTTCTGTCGCGCCTTTTCTCTCTCGGCGTCGAAGCGCGGGCAACCGTTTCCTCCGCAGC
CGGCCTCTCCTGGGCGGCCACCCGTTACGGCGATGCAGATGTCATCGCGCCGGGAGATGGAGATCCGCTCCTTGCGCCCT
TGCCGGTCGCCGCGTTGCGGCTGCCGGCGGAAACTGCTGCCGCCCTCGAACGGGTCGGGCTGAAGCAAATCGGCGATCTT
CTCGAAGCGCCGCGGGCGCCGCTCGCGCGCCGCTTCGGCTCCATCCTTCTTCTGAGGCTCGATCAGGCAAGGGGCTTGGA
GGAGGAGCCGCTTTCGCCCCGGCTGCCCGTGCCGAGTTTCTCGGCCGAGCGCCGGCTCGCCGAACCGCTGCAGGACGAGG
AATACATTCTCGAACTGACCCGGCATCTCGCCAGGGATGTCCGTTCCTCGCTGGAGCGGCATGGCGAAGGCGGGCGGCTC
TTCGAGCTTCTCCTGTTCCGCGTCGATGGCCGGGTATTTCGCGTCCGAGCGCACGCAGCCGTGCCCTTGAACGATGCCAT
CCGTATCGCCGCCCTCTTTCGCGAACGGCTGCAGGCGGTTCATGACGATCTGGATGCCGGGTATGGTTTCGAGATCGTCA
GGCTCTCGGTTCTCAGAAGCGAGAGGCTCGATCCTGCGCAACAGGATTTTTCCGGCGTTCCCGACGAAAGCCAGCCGCTG
GCGGCTTTCGTCGACAAGGTCTCCGCCCGCTTCGGGCCGGATTGTCTGATGCAGGCGACCCTTATGGAAAGCCACCTACC
GGAACGGGCCGGGGGATTTGCCGCCGTAACGGACGTGGCCGCGATCCTGGGATCACCTCCCCTTGGAGAGAGGGCTTTCC
CCGAAAACCGCCCGCTTCGGATTTTCGCGCATCCCGAACCGGTGGAGGCGACGGCCGAAGTACCCGAGGGCGCGCCGCGC
AGCTTCAACTGGCGCAAGACCCGGTATCGCGTTGCCCGTGCCGAGGGACCGGAGCGCGTCGCCGCCGAATGGTGGATCGA
TGGGGAGGGCCATCCGACGCGCGATTATTTTCGGATCGAGGACCAGGAGGGCCGCCGCTTCTGGCTGTTTCGCGAAGGCC
TTTATGGAAGAGAGACCGCCTCGCCCCGCTGGTTCATGCATGGAGTTTTCGCATGA

Upstream 100 bases:

>100_bases
GGCCGATCGCATCGCGCGCATTCGTTGGGGCGCCTCCTGGCTTTCCCGCGGGCGTCCTGACCATCCGCCCGTCGTCTTCG
CCGCCAAGCTCGACAATGCT

Downstream 100 bases:

>100_bases
GCGGGGATACTGCCTTCTACGAACTCGGTGCGTGCACGAATTTCTCTTTTCTCGAAGGGGCGGCGCCGGCCGAGGAAATG
GTCGTCTTCGCCAAGAAGGC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MRLVALDTVAERVGLKRGQGAAEARAMCPSLDVIAFDPAADQAFLEALADWCDRYTPLVALDGKDGLFLDITGCAHLHGG
EKALVSDVLSRLFSLGVEARATVSSAAGLSWAATRYGDADVIAPGDGDPLLAPLPVAALRLPAETAAALERVGLKQIGDL
LEAPRAPLARRFGSILLLRLDQARGLEEEPLSPRLPVPSFSAERRLAEPLQDEEYILELTRHLARDVRSSLERHGEGGRL
FELLLFRVDGRVFRVRAHAAVPLNDAIRIAALFRERLQAVHDDLDAGYGFEIVRLSVLRSERLDPAQQDFSGVPDESQPL
AAFVDKVSARFGPDCLMQATLMESHLPERAGGFAAVTDVAAILGSPPLGERAFPENRPLRIFAHPEPVEATAEVPEGAPR
SFNWRKTRYRVARAEGPERVAAEWWIDGEGHPTRDYFRIEDQEGRRFWLFREGLYGRETASPRWFMHGVFA

Sequences:

>Translated_471_residues
MRLVALDTVAERVGLKRGQGAAEARAMCPSLDVIAFDPAADQAFLEALADWCDRYTPLVALDGKDGLFLDITGCAHLHGG
EKALVSDVLSRLFSLGVEARATVSSAAGLSWAATRYGDADVIAPGDGDPLLAPLPVAALRLPAETAAALERVGLKQIGDL
LEAPRAPLARRFGSILLLRLDQARGLEEEPLSPRLPVPSFSAERRLAEPLQDEEYILELTRHLARDVRSSLERHGEGGRL
FELLLFRVDGRVFRVRAHAAVPLNDAIRIAALFRERLQAVHDDLDAGYGFEIVRLSVLRSERLDPAQQDFSGVPDESQPL
AAFVDKVSARFGPDCLMQATLMESHLPERAGGFAAVTDVAAILGSPPLGERAFPENRPLRIFAHPEPVEATAEVPEGAPR
SFNWRKTRYRVARAEGPERVAAEWWIDGEGHPTRDYFRIEDQEGRRFWLFREGLYGRETASPRWFMHGVFA
>Mature_471_residues
MRLVALDTVAERVGLKRGQGAAEARAMCPSLDVIAFDPAADQAFLEALADWCDRYTPLVALDGKDGLFLDITGCAHLHGG
EKALVSDVLSRLFSLGVEARATVSSAAGLSWAATRYGDADVIAPGDGDPLLAPLPVAALRLPAETAAALERVGLKQIGDL
LEAPRAPLARRFGSILLLRLDQARGLEEEPLSPRLPVPSFSAERRLAEPLQDEEYILELTRHLARDVRSSLERHGEGGRL
FELLLFRVDGRVFRVRAHAAVPLNDAIRIAALFRERLQAVHDDLDAGYGFEIVRLSVLRSERLDPAQQDFSGVPDESQPL
AAFVDKVSARFGPDCLMQATLMESHLPERAGGFAAVTDVAAILGSPPLGERAFPENRPLRIFAHPEPVEATAEVPEGAPR
SFNWRKTRYRVARAEGPERVAAEWWIDGEGHPTRDYFRIEDQEGRRFWLFREGLYGRETASPRWFMHGVFA

Specific function: Along with dnaE2 and imuA is required for the error- prone processing of DNA lesions [H]

COG id: COG0389

COG function: function code L; Nucleotidyltransferase/DNA polymerase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786425, Length=232, Percent_Identity=25.4310344827586, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001126 [H]

Pfam domain/function: PF00817 IMS [H]

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 51854; Mature: 51854

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVALDTVAERVGLKRGQGAAEARAMCPSLDVIAFDPAADQAFLEALADWCDRYTPLVA
CEEEEHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEE
LDGKDGLFLDITGCAHLHGGEKALVSDVLSRLFSLGVEARATVSSAAGLSWAATRYGDAD
ECCCCCEEEEEECEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHCCCCCC
VIAPGDGDPLLAPLPVAALRLPAETAAALERVGLKQIGDLLEAPRAPLARRFGSILLLRL
EECCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEE
DQARGLEEEPLSPRLPVPSFSAERRLAEPLQDEEYILELTRHLARDVRSSLERHGEGGRL
HHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
FELLLFRVDGRVFRVRAHAAVPLNDAIRIAALFRERLQAVHDDLDAGYGFEIVRLSVLRS
HHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ERLDPAQQDFSGVPDESQPLAAFVDKVSARFGPDCLMQATLMESHLPERAGGFAAVTDVA
HCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHCCCHHHHHHHH
AILGSPPLGERAFPENRPLRIFAHPEPVEATAEVPEGAPRSFNWRKTRYRVARAEGPERV
HHHCCCCCCCCCCCCCCCEEEEECCCCCCHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCE
AAEWWIDGEGHPTRDYFRIEDQEGRRFWLFREGLYGRETASPRWFMHGVFA
EEEEEECCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCHHHCCCCC
>Mature Secondary Structure
MRLVALDTVAERVGLKRGQGAAEARAMCPSLDVIAFDPAADQAFLEALADWCDRYTPLVA
CEEEEHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEE
LDGKDGLFLDITGCAHLHGGEKALVSDVLSRLFSLGVEARATVSSAAGLSWAATRYGDAD
ECCCCCEEEEEECEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHCCCCCC
VIAPGDGDPLLAPLPVAALRLPAETAAALERVGLKQIGDLLEAPRAPLARRFGSILLLRL
EECCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEE
DQARGLEEEPLSPRLPVPSFSAERRLAEPLQDEEYILELTRHLARDVRSSLERHGEGGRL
HHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
FELLLFRVDGRVFRVRAHAAVPLNDAIRIAALFRERLQAVHDDLDAGYGFEIVRLSVLRS
HHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ERLDPAQQDFSGVPDESQPLAAFVDKVSARFGPDCLMQATLMESHLPERAGGFAAVTDVA
HCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHCCCHHHHHHHH
AILGSPPLGERAFPENRPLRIFAHPEPVEATAEVPEGAPRSFNWRKTRYRVARAEGPERV
HHHCCCCCCCCCCCCCCCEEEEECCCCCCHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCE
AAEWWIDGEGHPTRDYFRIEDQEGRRFWLFREGLYGRETASPRWFMHGVFA
EEEEEECCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA