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The map label for this gene is rlmH [H]

Identifier: 150398209

GI number: 150398209

Start: 3162247

End: 3162729

Strand: Direct

Name: rlmH [H]

Synonym: Smed_3014

Alternate gene names: 150398209

Gene position: 3162247-3162729 (Clockwise)

Preceding gene: 150398208

Following gene: 150398210

Centisome position: 83.62

GC content: 67.29

Gene sequence:

>483_bases
ATGCGCATCGGACTTTTCGCAGTGGGCCGCCTCAAGGCGGGCCCGGAGAAGGATCTTGCCGTCCGCTATCTCGACCGGTT
CGCCAAGACGGGGCCGGCGGTCGGCCTCGAGCTGGCCCGCGTCGCCGAGATGGTTGAGAGCCGCGCTGCCAACGCCGAGA
CGCGCAAACGCGAGGAGGCCGTTCAGCTCGAAAAGGCACTCGTCGACGGCAGCCTTCTCGTGTTGCTCGACGAGCGCGGC
AAAGCGCTCGACTCGGAAGCCTTCGCGAACCTGCTCGCTACTTTCAGGGACAGCGGCAAACGCGATCTCATGATCGCGAT
CGGCGGCGCAGACGGCCTCGACCCGGCGCTCCACGCACGGGCGGACGCCGTGCTGTGCCTCGGCAAGATGACCTGGCCGC
ACCAGCTCGTTCGGATCCTTATCGCCGAGCAGCTCTACCGGGCCGTGACGATCCTCTCGGGGCACCCCTATCATCGGGCG
TGA

Upstream 100 bases:

>100_bases
TGAAATAAGGCTGCCACCGCCGATGAGGCGGATGGCGGCAAGAGTTGTTTTTGCCGGTTGAATTTGGCGTCGGCCGGCGC
CGGCTGGGACGGATCGGGTC

Downstream 100 bases:

>100_bases
TCAAGCGGAGAGCTTGCCACTCATCCCGCTGCCGCGACCGTTCTCCCCGTTTTGACGGGAAGAAGGGCAAGCCGAGAGCG
CCCGCTTCAAAACGGAGCAA

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MRIGLFAVGRLKAGPEKDLAVRYLDRFAKTGPAVGLELARVAEMVESRAANAETRKREEAVQLEKALVDGSLLVLLDERG
KALDSEAFANLLATFRDSGKRDLMIAIGGADGLDPALHARADAVLCLGKMTWPHQLVRILIAEQLYRAVTILSGHPYHRA

Sequences:

>Translated_160_residues
MRIGLFAVGRLKAGPEKDLAVRYLDRFAKTGPAVGLELARVAEMVESRAANAETRKREEAVQLEKALVDGSLLVLLDERG
KALDSEAFANLLATFRDSGKRDLMIAIGGADGLDPALHARADAVLCLGKMTWPHQLVRILIAEQLYRAVTILSGHPYHRA
>Mature_160_residues
MRIGLFAVGRLKAGPEKDLAVRYLDRFAKTGPAVGLELARVAEMVESRAANAETRKREEAVQLEKALVDGSLLVLLDERG
KALDSEAFANLLATFRDSGKRDLMIAIGGADGLDPALHARADAVLCLGKMTWPHQLVRILIAEQLYRAVTILSGHPYHRA

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA [H]

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family [H]

Homologues:

Organism=Escherichia coli, GI1786855, Length=161, Percent_Identity=36.6459627329193, Blast_Score=83, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003742 [H]

Pfam domain/function: PF02590 SPOUT_MTase [H]

EC number: NA

Molecular weight: Translated: 17442; Mature: 17442

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGLFAVGRLKAGPEKDLAVRYLDRFAKTGPAVGLELARVAEMVESRAANAETRKREEA
CCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
VQLEKALVDGSLLVLLDERGKALDSEAFANLLATFRDSGKRDLMIAIGGADGLDPALHAR
HHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHH
ADAVLCLGKMTWPHQLVRILIAEQLYRAVTILSGHPYHRA
HHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRIGLFAVGRLKAGPEKDLAVRYLDRFAKTGPAVGLELARVAEMVESRAANAETRKREEA
CCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
VQLEKALVDGSLLVLLDERGKALDSEAFANLLATFRDSGKRDLMIAIGGADGLDPALHAR
HHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHH
ADAVLCLGKMTWPHQLVRILIAEQLYRAVTILSGHPYHRA
HHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]