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The map label for this gene is glnH [H]

Identifier: 150398176

GI number: 150398176

Start: 3127146

End: 3127943

Strand: Direct

Name: glnH [H]

Synonym: Smed_2979

Alternate gene names: 150398176

Gene position: 3127146-3127943 (Clockwise)

Preceding gene: 150398170

Following gene: 150398177

Centisome position: 82.69

GC content: 60.28

Gene sequence:

>798_bases
ATGACACGCACATCCAAGCTCAAAGCCGCCATCGTTTCCACAGCCTTCGCTGCAACCGGAGCCTTCGCTGCCCAGGCTGC
CGACCTGACGGTCGGCGCCAATATCGGCAACGTTCCTTGGGAATTCCAGGAGGCGGACGGCAAGATCGTCGGCTTCGAGG
TCGACCTGGTCAACGAGATCGCGAAGCGGCTCGGCAAATCCGTGGAGTTCGTCAATACGCCATTCAACGGCCTTTTCCCG
GCCGTGCAGTCCGGACGCATCGACATGGCGATTTCGTCGATCACCATCACCGACAAGCGGCTGGAATCCGTCTCATTCGC
GCAGCCCTATTACGACAGCGACCAGTCACTCACCGTGACCGCGACTTCCGGCACCACCGGCTTGAAGGACATGGAGGGGA
AGGTCGTTGGCGTCGACACCGGCTCGACGGGCGACATGTGGGCCGACAGGAACAAGGCGGACTACAAGTTCGGGGAGATT
CGCCGCTTCGAGGGTCTGTCTCCGGCCATGCTCGATCTCGTCGCCGGCCGTGTCGACGGCTATATCAGCGACATTCCGGC
CCTGCTCTACTATGTGAAGGACAAGCCAGACCTGAAAGTCGTCGAGCGCATCCCGACCGGCGAGAAATACTCGATCATGT
TCAACAAGGGCGCTCCGCTTGCAGCCGAAGTGAACGACGTCATCACGACGCTGAAAGGCGAAGGATTTATCGCAAAACTG
CACGAGAGCTGGTTCGGTGCCAAGGCTGAGGATACGACCTCGACCGTCGCTGTACTGGACATGCCCGCGGCCGAGTAA

Upstream 100 bases:

>100_bases
AAAGCGCCTTGACCCGTGATTGTCTGGTCTAACTGGTCAGACCGGTCAGGCCACAATGTCAAGGTAAGTCGCGCCAGTTA
AAAAAAGCAAGGGGAACTTA

Downstream 100 bases:

>100_bases
CCCGCCGTTCCAAAGAATCGAGACGCGGGATGGGGGCGGAAAACCGCACATAGCTTCCTCATCCCGCTCTCGGGAGCCTT
CTCGGCCAAGGGAGCCTTGC

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MTRTSKLKAAIVSTAFAATGAFAAQAADLTVGANIGNVPWEFQEADGKIVGFEVDLVNEIAKRLGKSVEFVNTPFNGLFP
AVQSGRIDMAISSITITDKRLESVSFAQPYYDSDQSLTVTATSGTTGLKDMEGKVVGVDTGSTGDMWADRNKADYKFGEI
RRFEGLSPAMLDLVAGRVDGYISDIPALLYYVKDKPDLKVVERIPTGEKYSIMFNKGAPLAAEVNDVITTLKGEGFIAKL
HESWFGAKAEDTTSTVAVLDMPAAE

Sequences:

>Translated_265_residues
MTRTSKLKAAIVSTAFAATGAFAAQAADLTVGANIGNVPWEFQEADGKIVGFEVDLVNEIAKRLGKSVEFVNTPFNGLFP
AVQSGRIDMAISSITITDKRLESVSFAQPYYDSDQSLTVTATSGTTGLKDMEGKVVGVDTGSTGDMWADRNKADYKFGEI
RRFEGLSPAMLDLVAGRVDGYISDIPALLYYVKDKPDLKVVERIPTGEKYSIMFNKGAPLAAEVNDVITTLKGEGFIAKL
HESWFGAKAEDTTSTVAVLDMPAAE
>Mature_264_residues
TRTSKLKAAIVSTAFAATGAFAAQAADLTVGANIGNVPWEFQEADGKIVGFEVDLVNEIAKRLGKSVEFVNTPFNGLFPA
VQSGRIDMAISSITITDKRLESVSFAQPYYDSDQSLTVTATSGTTGLKDMEGKVVGVDTGSTGDMWADRNKADYKFGEIR
RFEGLSPAMLDLVAGRVDGYISDIPALLYYVKDKPDLKVVERIPTGEKYSIMFNKGAPLAAEVNDVITTLKGEGFIAKLH
ESWFGAKAEDTTSTVAVLDMPAAE

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=252, Percent_Identity=34.9206349206349, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1788228, Length=251, Percent_Identity=32.2709163346614, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787085, Length=245, Percent_Identity=26.530612244898, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787088, Length=247, Percent_Identity=27.1255060728745, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1788648, Length=158, Percent_Identity=31.6455696202532, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788649, Length=241, Percent_Identity=28.2157676348548, Blast_Score=87, Evalue=9e-19,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28426; Mature: 28295

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTSKLKAAIVSTAFAATGAFAAQAADLTVGANIGNVPWEFQEADGKIVGFEVDLVNEI
CCCCHHHHHHHHHHHHHHHCHHHHHHCCEEECCCCCCCCCEEECCCCCEEEEEHHHHHHH
AKRLGKSVEFVNTPFNGLFPAVQSGRIDMAISSITITDKRLESVSFAQPYYDSDQSLTVT
HHHHCCCEEEECCCCCCCCHHHCCCEEEEEEEEEEEEHHHHHHHHCCCCCCCCCCEEEEE
ATSGTTGLKDMEGKVVGVDTGSTGDMWADRNKADYKFGEIRRFEGLSPAMLDLVAGRVDG
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCC
YISDIPALLYYVKDKPDLKVVERIPTGEKYSIMFNKGAPLAAEVNDVITTLKGEGFIAKL
HHHHHHEEEEEECCCCCHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEE
HESWFGAKAEDTTSTVAVLDMPAAE
HHHHCCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure 
TRTSKLKAAIVSTAFAATGAFAAQAADLTVGANIGNVPWEFQEADGKIVGFEVDLVNEI
CCCHHHHHHHHHHHHHHHCHHHHHHCCEEECCCCCCCCCEEECCCCCEEEEEHHHHHHH
AKRLGKSVEFVNTPFNGLFPAVQSGRIDMAISSITITDKRLESVSFAQPYYDSDQSLTVT
HHHHCCCEEEECCCCCCCCHHHCCCEEEEEEEEEEEEHHHHHHHHCCCCCCCCCCEEEEE
ATSGTTGLKDMEGKVVGVDTGSTGDMWADRNKADYKFGEIRRFEGLSPAMLDLVAGRVDG
EECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCC
YISDIPALLYYVKDKPDLKVVERIPTGEKYSIMFNKGAPLAAEVNDVITTLKGEGFIAKL
HHHHHHEEEEEECCCCCHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEE
HESWFGAKAEDTTSTVAVLDMPAAE
HHHHCCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]