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The map label for this gene is priA [H]

Identifier: 150398124

GI number: 150398124

Start: 3071332

End: 3073536

Strand: Reverse

Name: priA [H]

Synonym: Smed_2927

Alternate gene names: 150398124

Gene position: 3073536-3071332 (Counterclockwise)

Preceding gene: 150398126

Following gene: 150398123

Centisome position: 81.27

GC content: 67.44

Gene sequence:

>2205_bases
ATGACTCTTGATTCAACCGATCTATTCGGCCCCGTACTGGACCGCCGCGTGGTGCCCGTCCTCGTGCCCATGCCGGCGCC
GAGGGCCTATTCCTACGCGGTGCCGGAGGGCATGGCGGTCGAGCCGGGGTCGATCGTCCAAGTGCCGCTCGGCCCCCGTT
TCGTCGTCGGCGTCGTCTGGGACGGCGAAGACGACGGCGGCGTCGACCCGAAGAAGCTGAGGGCGATCGAAAAGGTCTTC
GATTGCCCGCCGCTCAGCCCCGAGATGCGCGCCTTTCTCGATTGGGTTGCTGCCTACACGCTGACGCCGCCAGGCCTCGT
CGCCCGCATGGCCCTGCGGGCGCCTGCGGCTTTCGATCCGGAGCCGATGGTGGAAGCGCTGCGGCTGACCGACATGAGGC
CCGAGCGTATGACGACGGCGCGCGAGCGCGTCCTTGCCAGAGCGAGCGACGGACTATCCTGGACCCGCTCGGGGCTCGCG
CATGCCGCCGGGGTTTCGTCCAGCGTCATCGACGGGCTGGCGTCGCAGGGCGTGTTCGAAACCGTCTTCATGCCGCCGCC
ACCCGTCGTCGCGCTACCGGATCCAGCCTTCGCCGCGCCCCGCCTCGAAGGACCGCAAAGGCAGGCGGCTGTCGACCTGC
TGGCGGCCGTCGAGGAGCGGAAGTTCTCGGTTTCGCTGATCGACGGCATTACCGGCTCCGGCAAGACGGAGGTGTATTTC
GAGGCGATCGCGGCCACGCTGCGCGCCGGCAGACAGGTCCTGATCCTGCTGCCGGAGATCGCGCTTACCGCAAGCTTTCT
CGAACGCTTCCAGGACCGTTTCGGCGCCAAGCCGGCGGAGTGGCACTCCGATCTCGCGCCGCGCACGCGGGAGAAGGTCT
GGCGGCAGGTGACGACCGGTCAGGTGCGCGTCGTCGCCGGCGCCCGCTCGGCGTTGTTCCTGCCCTTCGAAGAACTCGGG
CTCATCATCGTCGACGAAGAGCACGACCCCGCATACAAGCAGGAGGACCGCGTTTTCTACAACGCGCGCGACATGGCTGT
CGTGCGCGGGCGCATCGGCAATTTTCCGGTTGTACTCGTCTCCGCCACGCCTTCCGTCGAGAGCCGGGTAAACGGTGAGA
TGGGCCGTTACCGGCCGATCCATCTGCCGACCCGTTACGGCGATGCGGCGCTGCCGGATCTCGGCATAGTCGACATGCGC
CGTCATCCGCCGGAACGCGGCGGCTTCCTCTCGCCGGTGCTCGTGAACCATATCGGCAAGACGATCGGCCGCAGCCAGCA
GGCGCTCCTCTTTCTCAATCGGCGCGGCTACGCACCGCTGACGCTCTGCCGTGTCTGCGGCCATCGTTTCCAGTGCCCGG
ACTGCTCGAGCTGGCTCGTCGAGCACCGCTTCCGCGGCCAGATCCAGTGCCATCATTGCGGCTACTCGGAGAGAACCCCT
GAAGCCTGTCCGGAATGCGGCACGCTCGACCATCTCGTTGCCTGCGGCCCCGGCGTCGAGCGCATTGCCGAAGAGGTCGA
ACGGCACTTTCCGGACGCGCGCACCATCGTGCTTTCCTCCGACCTGATGGGGGTCAAGCGGCTGCGGCTGGAGCTCGAGG
CGATCGCTCGCGGCGAGGCGGACATCGTCATCGGCACGCAGCTCGTTGCCAAAGGGCACAACTTCCCGAATATGACGCTG
GTCGGCATCGTCGATGCGGATCTGGGGCTTGCCAATGGCGACCCGCGCGCGGCCGAACGGACATTTCAGCTGCTTTCTCA
GGTGACTGGCCGCGCCGGCCGGACGGGCCTCAAGAGCCTCGGCCTTCTGCAGACCTATCAGCCGCAGCATCCGGTCATGC
AGGCGATCGTGTCGGGCGATTCCGATGCTTTCTACCAGCGCGAAATCCATGAGCGGGAAAGGGCGGCGCTGCCTCCGTTC
GGCCGGCTCGCCTCGATCATCGTTTCCGCCGACAGCCGCGGCGATGCCGAGGGCCATGCCCGCGGATTGCGCAACGCGGC
GCCGCGCGTCGACGGCATCGCGATCCTCGGACCGGCGGAAGCACCGCTCGCGCTCATCCGCGGCCGGCATCGCTTCCGGC
TGCTCGTCCACGGGCGGCGCAACAGCGACATGCAGACTTTCGTCCGGGCGATGATCGCGGCCGGCCCCAAGGAGCGCGGA
TCGATCAGCGTCCAACTCGACATCGATCCGCAGAGTTTCCTGTGA

Upstream 100 bases:

>100_bases
GTTGAAACTCGGTTGGAACCGTGACAAACCCTGCTTTCTGCAAGGCAGCGGTTCGCTGCGAGTTTTCTTTAACTGAAAGC
GGTGGCAAGGTCACGGCAAA

Downstream 100 bases:

>100_bases
TCACCGTCCTCCTTCGTCCTTCAACCGATTCACATCCGGGAGGACATAGGCTAGACCACTCCCACGCACTGCGCCGACTC
GGCGCGGGGCGATGACCCAC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 734; Mature: 733

Protein sequence:

>734_residues
MTLDSTDLFGPVLDRRVVPVLVPMPAPRAYSYAVPEGMAVEPGSIVQVPLGPRFVVGVVWDGEDDGGVDPKKLRAIEKVF
DCPPLSPEMRAFLDWVAAYTLTPPGLVARMALRAPAAFDPEPMVEALRLTDMRPERMTTARERVLARASDGLSWTRSGLA
HAAGVSSSVIDGLASQGVFETVFMPPPPVVALPDPAFAAPRLEGPQRQAAVDLLAAVEERKFSVSLIDGITGSGKTEVYF
EAIAATLRAGRQVLILLPEIALTASFLERFQDRFGAKPAEWHSDLAPRTREKVWRQVTTGQVRVVAGARSALFLPFEELG
LIIVDEEHDPAYKQEDRVFYNARDMAVVRGRIGNFPVVLVSATPSVESRVNGEMGRYRPIHLPTRYGDAALPDLGIVDMR
RHPPERGGFLSPVLVNHIGKTIGRSQQALLFLNRRGYAPLTLCRVCGHRFQCPDCSSWLVEHRFRGQIQCHHCGYSERTP
EACPECGTLDHLVACGPGVERIAEEVERHFPDARTIVLSSDLMGVKRLRLELEAIARGEADIVIGTQLVAKGHNFPNMTL
VGIVDADLGLANGDPRAAERTFQLLSQVTGRAGRTGLKSLGLLQTYQPQHPVMQAIVSGDSDAFYQREIHERERAALPPF
GRLASIIVSADSRGDAEGHARGLRNAAPRVDGIAILGPAEAPLALIRGRHRFRLLVHGRRNSDMQTFVRAMIAAGPKERG
SISVQLDIDPQSFL

Sequences:

>Translated_734_residues
MTLDSTDLFGPVLDRRVVPVLVPMPAPRAYSYAVPEGMAVEPGSIVQVPLGPRFVVGVVWDGEDDGGVDPKKLRAIEKVF
DCPPLSPEMRAFLDWVAAYTLTPPGLVARMALRAPAAFDPEPMVEALRLTDMRPERMTTARERVLARASDGLSWTRSGLA
HAAGVSSSVIDGLASQGVFETVFMPPPPVVALPDPAFAAPRLEGPQRQAAVDLLAAVEERKFSVSLIDGITGSGKTEVYF
EAIAATLRAGRQVLILLPEIALTASFLERFQDRFGAKPAEWHSDLAPRTREKVWRQVTTGQVRVVAGARSALFLPFEELG
LIIVDEEHDPAYKQEDRVFYNARDMAVVRGRIGNFPVVLVSATPSVESRVNGEMGRYRPIHLPTRYGDAALPDLGIVDMR
RHPPERGGFLSPVLVNHIGKTIGRSQQALLFLNRRGYAPLTLCRVCGHRFQCPDCSSWLVEHRFRGQIQCHHCGYSERTP
EACPECGTLDHLVACGPGVERIAEEVERHFPDARTIVLSSDLMGVKRLRLELEAIARGEADIVIGTQLVAKGHNFPNMTL
VGIVDADLGLANGDPRAAERTFQLLSQVTGRAGRTGLKSLGLLQTYQPQHPVMQAIVSGDSDAFYQREIHERERAALPPF
GRLASIIVSADSRGDAEGHARGLRNAAPRVDGIAILGPAEAPLALIRGRHRFRLLVHGRRNSDMQTFVRAMIAAGPKERG
SISVQLDIDPQSFL
>Mature_733_residues
TLDSTDLFGPVLDRRVVPVLVPMPAPRAYSYAVPEGMAVEPGSIVQVPLGPRFVVGVVWDGEDDGGVDPKKLRAIEKVFD
CPPLSPEMRAFLDWVAAYTLTPPGLVARMALRAPAAFDPEPMVEALRLTDMRPERMTTARERVLARASDGLSWTRSGLAH
AAGVSSSVIDGLASQGVFETVFMPPPPVVALPDPAFAAPRLEGPQRQAAVDLLAAVEERKFSVSLIDGITGSGKTEVYFE
AIAATLRAGRQVLILLPEIALTASFLERFQDRFGAKPAEWHSDLAPRTREKVWRQVTTGQVRVVAGARSALFLPFEELGL
IIVDEEHDPAYKQEDRVFYNARDMAVVRGRIGNFPVVLVSATPSVESRVNGEMGRYRPIHLPTRYGDAALPDLGIVDMRR
HPPERGGFLSPVLVNHIGKTIGRSQQALLFLNRRGYAPLTLCRVCGHRFQCPDCSSWLVEHRFRGQIQCHHCGYSERTPE
ACPECGTLDHLVACGPGVERIAEEVERHFPDARTIVLSSDLMGVKRLRLELEAIARGEADIVIGTQLVAKGHNFPNMTLV
GIVDADLGLANGDPRAAERTFQLLSQVTGRAGRTGLKSLGLLQTYQPQHPVMQAIVSGDSDAFYQREIHERERAALPPFG
RLASIIVSADSRGDAEGHARGLRNAAPRVDGIAILGPAEAPLALIRGRHRFRLLVHGRRNSDMQTFVRAMIAAGPKERGS
ISVQLDIDPQSFL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=735, Percent_Identity=33.469387755102, Blast_Score=378, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 80219; Mature: 80088

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDSTDLFGPVLDRRVVPVLVPMPAPRAYSYAVPEGMAVEPGSIVQVPLGPRFVVGVVW
CCCCCCHHHHHHHCCCCCEEEECCCCCCCEEEECCCCCEECCCCEEEECCCCEEEEEEEE
DGEDDGGVDPKKLRAIEKVFDCPPLSPEMRAFLDWVAAYTLTPPGLVARMALRAPAAFDP
ECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
EPMVEALRLTDMRPERMTTARERVLARASDGLSWTRSGLAHAAGVSSSVIDGLASQGVFE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCEE
TVFMPPPPVVALPDPAFAAPRLEGPQRQAAVDLLAAVEERKFSVSLIDGITGSGKTEVYF
EEECCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHH
EAIAATLRAGRQVLILLPEIALTASFLERFQDRFGAKPAEWHSDLAPRTREKVWRQVTTG
HHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCC
QVRVVAGARSALFLPFEELGLIIVDEEHDPAYKQEDRVFYNARDMAVVRGRIGNFPVVLV
CEEEEECCCCEEEEEHHHCCEEEEECCCCCCCCCCCCEEECCCCHHEECCCCCCCCEEEE
SATPSVESRVNGEMGRYRPIHLPTRYGDAALPDLGIVDMRRHPPERGGFLSPVLVNHIGK
ECCCCHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
TIGRSQQALLFLNRRGYAPLTLCRVCGHRFQCPDCSSWLVEHRFRGQIQCHHCGYSERTP
HHCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCC
EACPECGTLDHLVACGPGVERIAEEVERHFPDARTIVLSSDLMGVKRLRLELEAIARGEA
CCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCC
DIVIGTQLVAKGHNFPNMTLVGIVDADLGLANGDPRAAERTFQLLSQVTGRAGRTGLKSL
EEEEEHHHHCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
GLLQTYQPQHPVMQAIVSGDSDAFYQREIHERERAALPPFGRLASIIVSADSRGDAEGHA
CHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHEECCCCCCCHHHH
RGLRNAAPRVDGIAILGPAEAPLALIRGRHRFRLLVHGRRNSDMQTFVRAMIAAGPKERG
HHHHHCCCCCCCEEEECCCCCCHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCC
SISVQLDIDPQSFL
EEEEEEECCHHHCC
>Mature Secondary Structure 
TLDSTDLFGPVLDRRVVPVLVPMPAPRAYSYAVPEGMAVEPGSIVQVPLGPRFVVGVVW
CCCCCHHHHHHHCCCCCEEEECCCCCCCEEEECCCCCEECCCCEEEECCCCEEEEEEEE
DGEDDGGVDPKKLRAIEKVFDCPPLSPEMRAFLDWVAAYTLTPPGLVARMALRAPAAFDP
ECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
EPMVEALRLTDMRPERMTTARERVLARASDGLSWTRSGLAHAAGVSSSVIDGLASQGVFE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCEE
TVFMPPPPVVALPDPAFAAPRLEGPQRQAAVDLLAAVEERKFSVSLIDGITGSGKTEVYF
EEECCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHH
EAIAATLRAGRQVLILLPEIALTASFLERFQDRFGAKPAEWHSDLAPRTREKVWRQVTTG
HHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCC
QVRVVAGARSALFLPFEELGLIIVDEEHDPAYKQEDRVFYNARDMAVVRGRIGNFPVVLV
CEEEEECCCCEEEEEHHHCCEEEEECCCCCCCCCCCCEEECCCCHHEECCCCCCCCEEEE
SATPSVESRVNGEMGRYRPIHLPTRYGDAALPDLGIVDMRRHPPERGGFLSPVLVNHIGK
ECCCCHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
TIGRSQQALLFLNRRGYAPLTLCRVCGHRFQCPDCSSWLVEHRFRGQIQCHHCGYSERTP
HHCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCC
EACPECGTLDHLVACGPGVERIAEEVERHFPDARTIVLSSDLMGVKRLRLELEAIARGEA
CCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCC
DIVIGTQLVAKGHNFPNMTLVGIVDADLGLANGDPRAAERTFQLLSQVTGRAGRTGLKSL
EEEEEHHHHCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
GLLQTYQPQHPVMQAIVSGDSDAFYQREIHERERAALPPFGRLASIIVSADSRGDAEGHA
CHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHEECCCCCCCHHHH
RGLRNAAPRVDGIAILGPAEAPLALIRGRHRFRLLVHGRRNSDMQTFVRAMIAAGPKERG
HHHHHCCCCCCCEEEECCCCCCHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCC
SISVQLDIDPQSFL
EEEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]