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The map label for this gene is nuoE2 [H]

Identifier: 150398097

GI number: 150398097

Start: 3042910

End: 3043389

Strand: Reverse

Name: nuoE2 [H]

Synonym: Smed_2899

Alternate gene names: 150398097

Gene position: 3043389-3042910 (Counterclockwise)

Preceding gene: 150398098

Following gene: 150398096

Centisome position: 80.47

GC content: 64.79

Gene sequence:

>480_bases
GTGAATATCCATCTGCCGCGCGCAGACGTGACCGAACGGACGCTGGCGATCGTCGGCGAACTCAAGAGGCTCGAAGGCCC
GCTTATTCCCATTCTGCACGAGATTCAGGACGAGTTCGGCTACGTGCCTGAGGAGAGCCTGCCGGTGATCGCACAGGAGC
TCAATCTGTCGCGCGCCGAAGTCTATGGCGTCGTCACCTTCTATCACGATTTCCGCGAGCACCCGGCCGGACGCCATGTG
CTCAAGCTCTGTCGCGCCGAAGCCTGCCAGTCGATGGGCGGCGACCGGCTTGCCGAGCGCGCCAAGGCGCTTCTCGGGAT
CGATTTCCACGAGACGACGCCGGATGGCGCCGTCACGCTCGAGCCCGTTTACTGTCTTGGGCTCTGTTCCTGCTCGCCGT
CGGCGATGCTCGACGGCGAGCTTCATGCGCGGCTCGATGAGGCGGTGCTCGACGCGCTGGTCGCGGAGGCCCGCCAATGA

Upstream 100 bases:

>100_bases
AGCGTGAAATCTCCGATCTTTGCCAATTGGTTGAGACAGCGGCGCGACGACGGTCGAGAAAACTTGACCAATGCGGCCGC
GAGCCGGGAGGGCTCCTTCA

Downstream 100 bases:

>100_bases
CTGTAAGAATCTTTATTCCCCGGGATGCAGCAGCCTTGGCGCTCGGCGCCGAAAGCGTGGTGGAGGCGATTTCCGGCGAG
ATCGCCGCTCGCGCGCTCGA

Product: formate dehydrogenase subunit gamma

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E 2; NDH-1 subunit E 2 [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MNIHLPRADVTERTLAIVGELKRLEGPLIPILHEIQDEFGYVPEESLPVIAQELNLSRAEVYGVVTFYHDFREHPAGRHV
LKLCRAEACQSMGGDRLAERAKALLGIDFHETTPDGAVTLEPVYCLGLCSCSPSAMLDGELHARLDEAVLDALVAEARQ

Sequences:

>Translated_159_residues
MNIHLPRADVTERTLAIVGELKRLEGPLIPILHEIQDEFGYVPEESLPVIAQELNLSRAEVYGVVTFYHDFREHPAGRHV
LKLCRAEACQSMGGDRLAERAKALLGIDFHETTPDGAVTLEPVYCLGLCSCSPSAMLDGELHARLDEAVLDALVAEARQ
>Mature_159_residues
MNIHLPRADVTERTLAIVGELKRLEGPLIPILHEIQDEFGYVPEESLPVIAQELNLSRAEVYGVVTFYHDFREHPAGRHV
LKLCRAEACQSMGGDRLAERAKALLGIDFHETTPDGAVTLEPVYCLGLCSCSPSAMLDGELHARLDEAVLDALVAEARQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=132, Percent_Identity=31.0606060606061, Blast_Score=73, Evalue=1e-13,
Organism=Escherichia coli, GI1788621, Length=133, Percent_Identity=35.3383458646617, Blast_Score=79, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17561328, Length=113, Percent_Identity=35.3982300884956, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355353, Length=136, Percent_Identity=28.6764705882353, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI18859877, Length=120, Percent_Identity=32.5, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17513; Mature: 17513

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIHLPRADVTERTLAIVGELKRLEGPLIPILHEIQDEFGYVPEESLPVIAQELNLSRAE
CCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHH
VYGVVTFYHDFREHPAGRHVLKLCRAEACQSMGGDRLAERAKALLGIDFHETTPDGAVTL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEE
EPVYCLGLCSCSPSAMLDGELHARLDEAVLDALVAEARQ
CHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIHLPRADVTERTLAIVGELKRLEGPLIPILHEIQDEFGYVPEESLPVIAQELNLSRAE
CCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHH
VYGVVTFYHDFREHPAGRHVLKLCRAEACQSMGGDRLAERAKALLGIDFHETTPDGAVTL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEE
EPVYCLGLCSCSPSAMLDGELHARLDEAVLDALVAEARQ
CHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]