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The map label for this gene is nuoF2 [H]

Identifier: 150398096

GI number: 150398096

Start: 3041357

End: 3042913

Strand: Reverse

Name: nuoF2 [H]

Synonym: Smed_2898

Alternate gene names: 150398096

Gene position: 3042913-3041357 (Counterclockwise)

Preceding gene: 150398097

Following gene: 150398095

Centisome position: 80.46

GC content: 63.84

Gene sequence:

>1557_bases
ATGACTGTAAGAATCTTTATTCCCCGGGATGCAGCAGCCTTGGCGCTCGGCGCCGAAAGCGTGGTGGAGGCGATTTCCGG
CGAGATCGCCGCTCGCGCGCTCGACGCCACGATCGTCCGCAACGGCTCTCGCGGCATGCACTGGCTGGAGCCGCTGGTCG
AAATCGAAACGGCCGATGGGCGCGTCGCCTACGGGCCGGTGAAAGCGGGCGACGTGGCCTCGCTTTTCGATGCCGGGCTG
ACTTCGGGTGGTGCGCATCCGCTCTGTCTCGGCAAGACCGAGGAAATTCCGTTCCTCAAGCGACAGACGCGGCTTACCTT
TGCCCGCTGCGGTGTCATCGACCCGCTCTCGCTCGATGATTATCGGGCTCATGGAGGCCTGCGCGGCCTCGAAAGAGCAG
TCGTCATGCAGCCTGCGGCGATCGTTGCCGAGGTCACGGAAAGCGGTCTTCGTGGCCGCGGCGGTGCCGGCTTCCCGACC
GGGATCAAATGGAAGACCGTGCTCGAAACGCCGGGTGCGCGGAAATACATCGTCTGCAATGCCGACGAGGGTGACAGTGG
CACATTCGCAGACCGTATGATCATGGAAGGCGACCCCTTCGTGCTGATCGAGGGCATGGCGATCGCCGGCATCGCGACTG
GCGCAACCAAGGGCTACATCTATACGCGCTCGGAATATCCGCATGCGATCGCGGTGATGAGCGCAGCGGTCGAGATCGCC
CGGGCCGCCGGTGTGCTCGGCACCTCTGTGCTCGGCTCTGCGCATGCCTTCGATATGGAGGTTCGCACCGGCGCCGGCGC
CTATGTCTGCGGCGAGGAGACTTCGCTCCTGAACAGCCTCGAAGGCAAGCGCGGACTGGTGCGCGCCAAGCCGCCGCTGC
CGGCGCATAAGGGCCTTTTCGATCGCCCGACCGTCATCAACAACGTCATCTCGCTCGCGTCCGTACCGGTGATTCTCGAC
AAGGGTGCCGGCTACTATCGCGATTTCGGCATGGGCCGGTCGCGCGGAACGATCCCGCTGCAGATCGCCGGCAATGTAAG
GCATGGCGGGCTTTTCGAAACCGCTTTCGGCCTGACGCTTGGCGAGATCGTCGACGAGATCGGCGGCGGCACCGCGAGCG
GCAGACCCGTGAAGGCCGTGCAGGTCGGCGGTCCGCTTGGCGCCTATTTCCCACGGGCGCTGTTCGATACGCCATTCGAC
TACGAAGCCTTCGCCGCCAGGGACGGGCTTATCGGTCATGCCGGCATCGTCGTCTTCGACGACACGGCCGACATGCTGAA
GCAGGCGCGTTTCGCCATGGAATTCTGCGCCATAGAGAGCTGCGGCAAGTGCACGCCCTGCCGCATAGGTTCCATACGCG
GTGTCGAGGTTGCCGACGCGATCGCGGCCGGCATCGAGCCGGAAAAGAATCGAGTGCTGCTTGCCGATCTCTGCAACACG
ATGAAGTTCGGCTCTCTTTGCGCACTCGGCGGCTTTACGCCCTATCCGGTGATGAGCGCCATGACGCATTTCCCGGAGGA
TTTTTCACCAGTGCCGATGGTGGAGGCTGCGGAATGA

Upstream 100 bases:

>100_bases
TTGGGCTCTGTTCCTGCTCGCCGTCGGCGATGCTCGACGGCGAGCTTCATGCGCGGCTCGATGAGGCGGTGCTCGACGCG
CTGGTCGCGGAGGCCCGCCA

Downstream 100 bases:

>100_bases
TAATGGCGGCCATGGCACACGGAACCTTGACAGAGCCGGCTATCGCATCCGCCCCGAACGTAATTTCTCAGGAGTCCCGC
TAATGTCTCTCATTCATGAA

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MTVRIFIPRDAAALALGAESVVEAISGEIAARALDATIVRNGSRGMHWLEPLVEIETADGRVAYGPVKAGDVASLFDAGL
TSGGAHPLCLGKTEEIPFLKRQTRLTFARCGVIDPLSLDDYRAHGGLRGLERAVVMQPAAIVAEVTESGLRGRGGAGFPT
GIKWKTVLETPGARKYIVCNADEGDSGTFADRMIMEGDPFVLIEGMAIAGIATGATKGYIYTRSEYPHAIAVMSAAVEIA
RAAGVLGTSVLGSAHAFDMEVRTGAGAYVCGEETSLLNSLEGKRGLVRAKPPLPAHKGLFDRPTVINNVISLASVPVILD
KGAGYYRDFGMGRSRGTIPLQIAGNVRHGGLFETAFGLTLGEIVDEIGGGTASGRPVKAVQVGGPLGAYFPRALFDTPFD
YEAFAARDGLIGHAGIVVFDDTADMLKQARFAMEFCAIESCGKCTPCRIGSIRGVEVADAIAAGIEPEKNRVLLADLCNT
MKFGSLCALGGFTPYPVMSAMTHFPEDFSPVPMVEAAE

Sequences:

>Translated_518_residues
MTVRIFIPRDAAALALGAESVVEAISGEIAARALDATIVRNGSRGMHWLEPLVEIETADGRVAYGPVKAGDVASLFDAGL
TSGGAHPLCLGKTEEIPFLKRQTRLTFARCGVIDPLSLDDYRAHGGLRGLERAVVMQPAAIVAEVTESGLRGRGGAGFPT
GIKWKTVLETPGARKYIVCNADEGDSGTFADRMIMEGDPFVLIEGMAIAGIATGATKGYIYTRSEYPHAIAVMSAAVEIA
RAAGVLGTSVLGSAHAFDMEVRTGAGAYVCGEETSLLNSLEGKRGLVRAKPPLPAHKGLFDRPTVINNVISLASVPVILD
KGAGYYRDFGMGRSRGTIPLQIAGNVRHGGLFETAFGLTLGEIVDEIGGGTASGRPVKAVQVGGPLGAYFPRALFDTPFD
YEAFAARDGLIGHAGIVVFDDTADMLKQARFAMEFCAIESCGKCTPCRIGSIRGVEVADAIAAGIEPEKNRVLLADLCNT
MKFGSLCALGGFTPYPVMSAMTHFPEDFSPVPMVEAAE
>Mature_517_residues
TVRIFIPRDAAALALGAESVVEAISGEIAARALDATIVRNGSRGMHWLEPLVEIETADGRVAYGPVKAGDVASLFDAGLT
SGGAHPLCLGKTEEIPFLKRQTRLTFARCGVIDPLSLDDYRAHGGLRGLERAVVMQPAAIVAEVTESGLRGRGGAGFPTG
IKWKTVLETPGARKYIVCNADEGDSGTFADRMIMEGDPFVLIEGMAIAGIATGATKGYIYTRSEYPHAIAVMSAAVEIAR
AAGVLGTSVLGSAHAFDMEVRTGAGAYVCGEETSLLNSLEGKRGLVRAKPPLPAHKGLFDRPTVINNVISLASVPVILDK
GAGYYRDFGMGRSRGTIPLQIAGNVRHGGLFETAFGLTLGEIVDEIGGGTASGRPVKAVQVGGPLGAYFPRALFDTPFDY
EAFAARDGLIGHAGIVVFDDTADMLKQARFAMEFCAIESCGKCTPCRIGSIRGVEVADAIAAGIEPEKNRVLLADLCNTM
KFGSLCALGGFTPYPVMSAMTHFPEDFSPVPMVEAAE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=396, Percent_Identity=37.6262626262626, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI20149568, Length=396, Percent_Identity=37.6262626262626, Blast_Score=247, Evalue=2e-65,
Organism=Escherichia coli, GI1788620, Length=399, Percent_Identity=38.5964912280702, Blast_Score=285, Evalue=5e-78,
Organism=Caenorhabditis elegans, GI17531727, Length=398, Percent_Identity=38.1909547738693, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI19920794, Length=397, Percent_Identity=37.5314861460957, Blast_Score=245, Evalue=6e-65,
Organism=Drosophila melanogaster, GI45550452, Length=367, Percent_Identity=36.7847411444142, Blast_Score=225, Evalue=7e-59,
Organism=Drosophila melanogaster, GI24653819, Length=347, Percent_Identity=30.835734870317, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24653817, Length=342, Percent_Identity=27.1929824561404, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54401; Mature: 54270

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRIFIPRDAAALALGAESVVEAISGEIAARALDATIVRNGSRGMHWLEPLVEIETADG
CEEEEEECCCHHHHHHCHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHEEECCCC
RVAYGPVKAGDVASLFDAGLTSGGAHPLCLGKTEEIPFLKRQTRLTFARCGVIDPLSLDD
EEEECCCCCCCHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
YRAHGGLRGLERAVVMQPAAIVAEVTESGLRGRGGAGFPTGIKWKTVLETPGARKYIVCN
HHHCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEE
ADEGDSGTFADRMIMEGDPFVLIEGMAIAGIATGATKGYIYTRSEYPHAIAVMSAAVEIA
CCCCCCCCHHHHEEECCCCEEEEECCEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHH
RAAGVLGTSVLGSAHAFDMEVRTGAGAYVCGEETSLLNSLEGKRGLVRAKPPLPAHKGLF
HHHHHHHHHHHCCCEEEEEEEECCCCEEEECCHHHHHHHCCCCCCCEECCCCCCCCCCCC
DRPTVINNVISLASVPVILDKGAGYYRDFGMGRSRGTIPLQIAGNVRHGGLFETAFGLTL
CCCHHHHHHHHHHCCCEEEECCCCHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHH
GEIVDEIGGGTASGRPVKAVQVGGPLGAYFPRALFDTPFDYEAFAARDGLIGHAGIVVFD
HHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHCCCCEECCCEEEEC
DTADMLKQARFAMEFCAIESCGKCTPCRIGSIRGVEVADAIAAGIEPEKNRVLLADLCNT
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEHHHHHHH
MKFGSLCALGGFTPYPVMSAMTHFPEDFSPVPMVEAAE
HHHCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TVRIFIPRDAAALALGAESVVEAISGEIAARALDATIVRNGSRGMHWLEPLVEIETADG
EEEEEECCCHHHHHHCHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHEEECCCC
RVAYGPVKAGDVASLFDAGLTSGGAHPLCLGKTEEIPFLKRQTRLTFARCGVIDPLSLDD
EEEECCCCCCCHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
YRAHGGLRGLERAVVMQPAAIVAEVTESGLRGRGGAGFPTGIKWKTVLETPGARKYIVCN
HHHCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEE
ADEGDSGTFADRMIMEGDPFVLIEGMAIAGIATGATKGYIYTRSEYPHAIAVMSAAVEIA
CCCCCCCCHHHHEEECCCCEEEEECCEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHH
RAAGVLGTSVLGSAHAFDMEVRTGAGAYVCGEETSLLNSLEGKRGLVRAKPPLPAHKGLF
HHHHHHHHHHHCCCEEEEEEEECCCCEEEECCHHHHHHHCCCCCCCEECCCCCCCCCCCC
DRPTVINNVISLASVPVILDKGAGYYRDFGMGRSRGTIPLQIAGNVRHGGLFETAFGLTL
CCCHHHHHHHHHHCCCEEEECCCCHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHH
GEIVDEIGGGTASGRPVKAVQVGGPLGAYFPRALFDTPFDYEAFAARDGLIGHAGIVVFD
HHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHCCCCEECCCEEEEC
DTADMLKQARFAMEFCAIESCGKCTPCRIGSIRGVEVADAIAAGIEPEKNRVLLADLCNT
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEHHHHHHH
MKFGSLCALGGFTPYPVMSAMTHFPEDFSPVPMVEAAE
HHHCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]