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The map label for this gene is cobF [H]

Identifier: 150398017

GI number: 150398017

Start: 2943849

End: 2944619

Strand: Reverse

Name: cobF [H]

Synonym: Smed_2819

Alternate gene names: 150398017

Gene position: 2944619-2943849 (Counterclockwise)

Preceding gene: 150398018

Following gene: 150398016

Centisome position: 77.86

GC content: 61.35

Gene sequence:

>771_bases
ATGCGGCACATCCTGATCATCGGTATCGGCGCGGGTAACCCCGAGCACATCACGGTCCAGGCGATCAATGCGCTGAACAG
AGCCGATGTGCTGTTCATCCCGACGAAGGGTGCGAAGAGGTCCGAACTCGCCGAGCTTCGCCGCGAGATCTGTGCCCGCT
ACGTGACGAGGGCCGACAGCCGGACCATTGAATTCGCCGTGCCCGTTCGCCGAACCGAAGGGCGGGCGTATATCGAAAGC
GTGGACGACTGGCATGCGAGTATTGCCGCCACTTACGAGGCTCTCCTCGAGAGCGAGCTTGCCGATGGACAGACCGGCGC
GTTTCTTGTCTGGGGCGATCCGATGCTCTATGACAGCACGATCCGCATCGTCGAGCGCGTGAAGGCGCGCGGCCGCATTG
GCTTCGGCTTTGACGTCATTCCGGGCATTACCAGCCTGCAGGCGCTCTGCGCCAGCCATCGCATTCCGTTGAACCTCGTC
GGCAAACCGGTGGAAATCACCACCGGCCGGAGGCTCGCCGAGAGCTTTCCGCACAGGTGCGAGACGGCGGTCGTCATGCT
CGACGGCGAGCAGGCCTTTCAGAAGATCGAGGATCCCGACGCCGAGATCTATTGGGGCGCCTATCTCGGCAGCCCCGAAG
AGATCGTCATTTCAGGGCGCCTTGCCGACGTCAAAGAGGAAATTCTGAAAACCCGTGCTGCGGCACGCGAGCGGGTAGGC
TGGATCATGGACATATATCTTTTGCGAAAAGGCGCCGATTTCGACGAATAG

Upstream 100 bases:

>100_bases
ACGCCCTCGTTCCGGCGCCCGTCGCGAATGGACCTTGCGCCCCCGGCTCCGATCCGTCAAAGACTGGCCCGAATAGGCAG
GCGACGCGATGGGAGACGGC

Downstream 100 bases:

>100_bases
AGCAATTTCGGAAAAGGTGTAAAACGGCCTTAATCCGAATGCTTGAACGCTCTCAAGGCATGCGCCTGTGCTATAGGTTT
CACCGGAAAACTGGCTGGAC

Product: precorrin 6A synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MRHILIIGIGAGNPEHITVQAINALNRADVLFIPTKGAKRSELAELRREICARYVTRADSRTIEFAVPVRRTEGRAYIES
VDDWHASIAATYEALLESELADGQTGAFLVWGDPMLYDSTIRIVERVKARGRIGFGFDVIPGITSLQALCASHRIPLNLV
GKPVEITTGRRLAESFPHRCETAVVMLDGEQAFQKIEDPDAEIYWGAYLGSPEEIVISGRLADVKEEILKTRAAARERVG
WIMDIYLLRKGADFDE

Sequences:

>Translated_256_residues
MRHILIIGIGAGNPEHITVQAINALNRADVLFIPTKGAKRSELAELRREICARYVTRADSRTIEFAVPVRRTEGRAYIES
VDDWHASIAATYEALLESELADGQTGAFLVWGDPMLYDSTIRIVERVKARGRIGFGFDVIPGITSLQALCASHRIPLNLV
GKPVEITTGRRLAESFPHRCETAVVMLDGEQAFQKIEDPDAEIYWGAYLGSPEEIVISGRLADVKEEILKTRAAARERVG
WIMDIYLLRKGADFDE
>Mature_256_residues
MRHILIIGIGAGNPEHITVQAINALNRADVLFIPTKGAKRSELAELRREICARYVTRADSRTIEFAVPVRRTEGRAYIES
VDDWHASIAATYEALLESELADGQTGAFLVWGDPMLYDSTIRIVERVKARGRIGFGFDVIPGITSLQALCASHRIPLNLV
GKPVEITTGRRLAESFPHRCETAVVMLDGEQAFQKIEDPDAEIYWGAYLGSPEEIVISGRLADVKEEILKTRAAARERVG
WIMDIYLLRKGADFDE

Specific function: Catalyzes the methylation of C-1 in precorrin-5 and the subsequent extrusion of acetic acid from the resulting intermediate to form cobalt-precorrin-6A [H]

COG id: COG2243

COG function: function code H; Precorrin-2 methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR012797 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.152 [H]

Molecular weight: Translated: 28506; Mature: 28506

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHILIIGIGAGNPEHITVQAINALNRADVLFIPTKGAKRSELAELRREICARYVTRADS
CCEEEEEEECCCCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCC
RTIEFAVPVRRTEGRAYIESVDDWHASIAATYEALLESELADGQTGAFLVWGDPMLYDST
CEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEHHHH
IRIVERVKARGRIGFGFDVIPGITSLQALCASHRIPLNLVGKPVEITTGRRLAESFPHRC
HHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHCCCCCEECCCCEEECCCHHHHHHCCCCC
ETAVVMLDGEQAFQKIEDPDAEIYWGAYLGSPEEIVISGRLADVKEEILKTRAAARERVG
CEEEEEEECHHHHHHCCCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
WIMDIYLLRKGADFDE
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRHILIIGIGAGNPEHITVQAINALNRADVLFIPTKGAKRSELAELRREICARYVTRADS
CCEEEEEEECCCCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCC
RTIEFAVPVRRTEGRAYIESVDDWHASIAATYEALLESELADGQTGAFLVWGDPMLYDST
CEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEHHHH
IRIVERVKARGRIGFGFDVIPGITSLQALCASHRIPLNLVGKPVEITTGRRLAESFPHRC
HHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHCCCCCEECCCCEEECCCHHHHHHCCCCC
ETAVVMLDGEQAFQKIEDPDAEIYWGAYLGSPEEIVISGRLADVKEEILKTRAAARERVG
CEEEEEEECHHHHHHCCCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
WIMDIYLLRKGADFDE
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2211521 [H]