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The map label for this gene is eda [H]

Identifier: 150397977

GI number: 150397977

Start: 2897734

End: 2898372

Strand: Reverse

Name: eda [H]

Synonym: Smed_2779

Alternate gene names: 150397977

Gene position: 2898372-2897734 (Counterclockwise)

Preceding gene: 150397981

Following gene: 150397976

Centisome position: 76.64

GC content: 66.51

Gene sequence:

>639_bases
ATGAGCGCGAAAACGGACAAGCTTCTATCCATCCTCAAGCTGCAGCCGGTGGTTCCGGTGCTGGTGATCGATGACGCGGC
GTCGGCGGTTCCGCTCGCGCGAGCCCTGGTCGCCGGCGGCCTCAAGGCGATCGAGATCACCTTGCGCACACCGGCCGCGC
TGGAAGCGATCCGCGCGGTTGCAGGAGAGGTGGAGGGCGCCGTGGCCGGAGCCGGGACCATCCTCAACGCGGCCCAGTTC
GAAGAGGCTGTCACGGCCGGATCGCAGTTCATCGTAAGCCCCGGAACGACCCAGGAACTGATCGATGTCGCCAACGACCA
CGAGGTGCCGCTGCTTCCGGGCGCGGCCACGGCGAGCGAGGTCATGGGCCTGCGTGAGGAAGGCTACGGCGTAATGAAGT
TCTTCCCCGCCGAGCAGGCCGGCGGTGCCGCCTATCTGAAGTCGCTCTCATCGCCGCTTGCCGGCACCTTGTTCTGCCCA
ACCGGCGGCATTTCGCTTGCCAATGCACGCGACTATCTGACGCTTCCGAACGTGGTGTGCGTCGGCGGCTCCTGGGTTGC
GCCAAAGGATCTGGTCGCCCGAGGGGACTGGGTCGGTATCACGAAGCTGGCGGCCGAGGCCTTCGCGCTCAAGGGCTGA

Upstream 100 bases:

>100_bases
CATCGGGGCACAAGCGCCATCATAGCTTTCAGGCTCGACAAGGGCGGATCGGCACGGTATGCGAGGTGCATTATTCAATC
GTTTTAAAAGGACGATCGGC

Downstream 100 bases:

>100_bases
GGCCGGCCGGCACGCGATCGAGGACGGCTGTTGTCCGGCCGTTTCATTTTCCAAGCCGGTTTGTATTTCCGCGGTGCCGT
TCCTATGTCTCAATCGGCCG

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MSAKTDKLLSILKLQPVVPVLVIDDAASAVPLARALVAGGLKAIEITLRTPAALEAIRAVAGEVEGAVAGAGTILNAAQF
EEAVTAGSQFIVSPGTTQELIDVANDHEVPLLPGAATASEVMGLREEGYGVMKFFPAEQAGGAAYLKSLSSPLAGTLFCP
TGGISLANARDYLTLPNVVCVGGSWVAPKDLVARGDWVGITKLAAEAFALKG

Sequences:

>Translated_212_residues
MSAKTDKLLSILKLQPVVPVLVIDDAASAVPLARALVAGGLKAIEITLRTPAALEAIRAVAGEVEGAVAGAGTILNAAQF
EEAVTAGSQFIVSPGTTQELIDVANDHEVPLLPGAATASEVMGLREEGYGVMKFFPAEQAGGAAYLKSLSSPLAGTLFCP
TGGISLANARDYLTLPNVVCVGGSWVAPKDLVARGDWVGITKLAAEAFALKG
>Mature_211_residues
SAKTDKLLSILKLQPVVPVLVIDDAASAVPLARALVAGGLKAIEITLRTPAALEAIRAVAGEVEGAVAGAGTILNAAQFE
EAVTAGSQFIVSPGTTQELIDVANDHEVPLLPGAATASEVMGLREEGYGVMKFFPAEQAGGAAYLKSLSSPLAGTLFCPT
GGISLANARDYLTLPNVVCVGGSWVAPKDLVARGDWVGITKLAAEAFALKG

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=180, Percent_Identity=51.6666666666667, Blast_Score=187, Evalue=6e-49,
Organism=Escherichia coli, GI48994954, Length=141, Percent_Identity=31.2056737588652, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.2.14 [H]

Molecular weight: Translated: 21551; Mature: 21420

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKTDKLLSILKLQPVVPVLVIDDAASAVPLARALVAGGLKAIEITLRTPAALEAIRAV
CCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHH
AGEVEGAVAGAGTILNAAQFEEAVTAGSQFIVSPGTTQELIDVANDHEVPLLPGAATASE
HHHHCCHHCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCHHH
VMGLREEGYGVMKFFPAEQAGGAAYLKSLSSPLAGTLFCPTGGISLANARDYLTLPNVVC
HHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEECCCCCCEECCCCCCEECCCEEE
VGGSWVAPKDLVARGDWVGITKLAAEAFALKG
ECCCCCCCHHHHHCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAKTDKLLSILKLQPVVPVLVIDDAASAVPLARALVAGGLKAIEITLRTPAALEAIRAV
CCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHH
AGEVEGAVAGAGTILNAAQFEEAVTAGSQFIVSPGTTQELIDVANDHEVPLLPGAATASE
HHHHCCHHCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCHHH
VMGLREEGYGVMKFFPAEQAGGAAYLKSLSSPLAGTLFCPTGGISLANARDYLTLPNVVC
HHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEECCCCCCEECCCCCCEECCCEEE
VGGSWVAPKDLVARGDWVGITKLAAEAFALKG
ECCCCCCCHHHHHCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]