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The map label for this gene is frmA [H]

Identifier: 150397945

GI number: 150397945

Start: 2864933

End: 2866144

Strand: Reverse

Name: frmA [H]

Synonym: Smed_2747

Alternate gene names: 150397945

Gene position: 2866144-2864933 (Counterclockwise)

Preceding gene: 150397947

Following gene: 150397932

Centisome position: 75.79

GC content: 64.69

Gene sequence:

>1212_bases
GTGAAGACGGGCTCTCCCGCATCTCGACTTACGGGAGACAATGGCATCTATATTTCCTCGCACGAACTGCAAACGGGAGG
AAAGAGCTTGAAGACACGTGCGGCGCTGCTGAGGACATCACCTGTCGGGAGGCCCTACGCCCATACGAGGCCGCTTTCGA
TCGAGACGATCGAGCTCGATCCGCCGGGGCCGGATGAATTGCTCGTGAAGATCGGGGCCGCCGGCCTCTGTCACTCCGAC
CTGTCGGTCATCAATGGCGACCGGCCGCGGCCGGTGCCGATGGTGCTTGGGCACGAGGCCGCCGGGACGGTAGAGGCGGT
CGGAAGCAATGTGCATGATCTCGCCGCCGGCGACCGGGTGGTGATGAGCTTCATGCCGAGTTGCGGGCATTGTCTGCCCT
GCGCGGAAGGGCGGCCGGCACTCTGCCTTCCCGGAGCGGAAGCGAACGGCAAGGGCACTCTGCTTTCCGGCGAGCGCCGG
CTGCATTGCCAGGACGTTCCGGTCAACCATCACCTCGGTGTTTCGGGTTTTGCCGATTATTCGGTGGTTTCCCGCCAATC
CGTCGTCAAGATCGAGGCGGATCTGCCGTTGGTCGAGGCGGCGCTGTTCGGCTGTGCCGTGCTCACCGGAGTCGGGGCGA
TCGTCAATACGTGCCGCGTGCGGCCGGGCGAATCCGTTGCGGTCGTCGGGCTGGGCGGGGTAGGGCTGGCGGCTCTTCTC
GGTGCTGTCGCCGCCGGTGCCGAGCGCATCGTCGCGGTGGATCTCGCGGAGGACAAGCTGAAACTCGCCCTTTCGCTCGG
TGCCACGGATACCGTCCTGGCGGCAGACTCTGACGCGGCCGAAAAAATCCGCCTCGCGACCCGTGGCGGCGTCGATCATG
CGGTCGAGGCGGCGGGCTCCGCCAAGGCCTTCGAACTTGCCTATCACATCACACGGAGAGGAGGGCTGACGGTTTCCGCG
GGGCTTGCTCATCCTTCGAGCCAGATCTCCATCCCGGCAGTGAGCCTCGTTGCGGAAGAGCGCAGTATGCGCGGAAGTTA
CATGGGAAGCTGTGTGCCCGCGCGCGACATTCCGCGGTTCATCGCGCTCTATCAGCGCGGCAGGCTGCCCGTCGACCGGT
TACTGTCCAGCACGGGTCCGCTTGAGGAGATCAACGAAGCCTTCGACCTTCTGGATCAGGGCCGCGTGATCAGACACGTG
ATAGAGTTCTGA

Upstream 100 bases:

>100_bases
AAAACTTATTCAGGGCAACACGATCTGTTCCGGCCAGTCGTTGCTCGATTGGCGGTCGGCCTCTCAACCGGGCTCGCTAA
CGGTCCGCAGCAGGGGGAGA

Downstream 100 bases:

>100_bases
TCACTTCACCATTGTCACGGAAATCATGTCGGCCATCCCTGCCAGGCGACCCAGCGTCCGTGAAAGTCGGCACGGCCGAC
GAGATGCTTGTCGCCGCTCG

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MKTGSPASRLTGDNGIYISSHELQTGGKSLKTRAALLRTSPVGRPYAHTRPLSIETIELDPPGPDELLVKIGAAGLCHSD
LSVINGDRPRPVPMVLGHEAAGTVEAVGSNVHDLAAGDRVVMSFMPSCGHCLPCAEGRPALCLPGAEANGKGTLLSGERR
LHCQDVPVNHHLGVSGFADYSVVSRQSVVKIEADLPLVEAALFGCAVLTGVGAIVNTCRVRPGESVAVVGLGGVGLAALL
GAVAAGAERIVAVDLAEDKLKLALSLGATDTVLAADSDAAEKIRLATRGGVDHAVEAAGSAKAFELAYHITRRGGLTVSA
GLAHPSSQISIPAVSLVAEERSMRGSYMGSCVPARDIPRFIALYQRGRLPVDRLLSSTGPLEEINEAFDLLDQGRVIRHV
IEF

Sequences:

>Translated_403_residues
MKTGSPASRLTGDNGIYISSHELQTGGKSLKTRAALLRTSPVGRPYAHTRPLSIETIELDPPGPDELLVKIGAAGLCHSD
LSVINGDRPRPVPMVLGHEAAGTVEAVGSNVHDLAAGDRVVMSFMPSCGHCLPCAEGRPALCLPGAEANGKGTLLSGERR
LHCQDVPVNHHLGVSGFADYSVVSRQSVVKIEADLPLVEAALFGCAVLTGVGAIVNTCRVRPGESVAVVGLGGVGLAALL
GAVAAGAERIVAVDLAEDKLKLALSLGATDTVLAADSDAAEKIRLATRGGVDHAVEAAGSAKAFELAYHITRRGGLTVSA
GLAHPSSQISIPAVSLVAEERSMRGSYMGSCVPARDIPRFIALYQRGRLPVDRLLSSTGPLEEINEAFDLLDQGRVIRHV
IEF
>Mature_403_residues
MKTGSPASRLTGDNGIYISSHELQTGGKSLKTRAALLRTSPVGRPYAHTRPLSIETIELDPPGPDELLVKIGAAGLCHSD
LSVINGDRPRPVPMVLGHEAAGTVEAVGSNVHDLAAGDRVVMSFMPSCGHCLPCAEGRPALCLPGAEANGKGTLLSGERR
LHCQDVPVNHHLGVSGFADYSVVSRQSVVKIEADLPLVEAALFGCAVLTGVGAIVNTCRVRPGESVAVVGLGGVGLAALL
GAVAAGAERIVAVDLAEDKLKLALSLGATDTVLAADSDAAEKIRLATRGGVDHAVEAAGSAKAFELAYHITRRGGLTVSA
GLAHPSSQISIPAVSLVAEERSMRGSYMGSCVPARDIPRFIALYQRGRLPVDRLLSSTGPLEEINEAFDLLDQGRVIRHV
IEF

Specific function: Has High Formaldehyde Dehydrogenase Activity In The Presence Of Glutathione And Catalyzes The Oxidation Of Normal Alcohols In A Reaction That Is Not Gsh-Dependent. In Addition, Hemithiolacetals Other Than Those Formed From Gsh, Including Omega-Thiol Fatty

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI34577061, Length=386, Percent_Identity=38.860103626943, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI4501933, Length=386, Percent_Identity=38.3419689119171, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI4501929, Length=386, Percent_Identity=37.3056994818653, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI71565154, Length=357, Percent_Identity=36.9747899159664, Blast_Score=232, Evalue=6e-61,
Organism=Homo sapiens, GI71743840, Length=386, Percent_Identity=33.9378238341969, Blast_Score=228, Evalue=6e-60,
Organism=Homo sapiens, GI71565152, Length=389, Percent_Identity=34.7043701799486, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI262073058, Length=379, Percent_Identity=33.7730870712401, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI156523966, Length=384, Percent_Identity=34.8958333333333, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI4501939, Length=378, Percent_Identity=34.9206349206349, Blast_Score=214, Evalue=9e-56,
Organism=Escherichia coli, GI1786552, Length=378, Percent_Identity=36.7724867724868, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1788407, Length=330, Percent_Identity=29.0909090909091, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87081918, Length=340, Percent_Identity=32.0588235294118, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI87082125, Length=363, Percent_Identity=25.6198347107438, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790045, Length=341, Percent_Identity=28.1524926686217, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788073, Length=367, Percent_Identity=27.2479564032698, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786825, Length=364, Percent_Identity=28.2967032967033, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI226510992, Length=360, Percent_Identity=27.2222222222222, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788075, Length=354, Percent_Identity=23.728813559322, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787863, Length=361, Percent_Identity=24.6537396121884, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082401, Length=362, Percent_Identity=27.0718232044199, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786518, Length=366, Percent_Identity=27.8688524590164, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25146526, Length=387, Percent_Identity=33.8501291989664, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI71997431, Length=389, Percent_Identity=33.6760925449871, Blast_Score=224, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI17565904, Length=344, Percent_Identity=33.1395348837209, Blast_Score=200, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI17562876, Length=363, Percent_Identity=28.9256198347107, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17562584, Length=362, Percent_Identity=29.5580110497238, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71988145, Length=378, Percent_Identity=28.5714285714286, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17562878, Length=361, Percent_Identity=29.0858725761773, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17562582, Length=359, Percent_Identity=28.133704735376, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320033, Length=382, Percent_Identity=35.0785340314136, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6323729, Length=380, Percent_Identity=27.3684210526316, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6319621, Length=369, Percent_Identity=27.10027100271, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323961, Length=364, Percent_Identity=27.7472527472527, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324486, Length=364, Percent_Identity=27.4725274725275, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6319257, Length=347, Percent_Identity=26.8011527377522, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6322619, Length=357, Percent_Identity=28.2913165266106, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319258, Length=308, Percent_Identity=25.3246753246753, Blast_Score=76, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6319955, Length=357, Percent_Identity=28.0112044817927, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI17737895, Length=387, Percent_Identity=37.2093023255814, Blast_Score=242, Evalue=3e-64,
Organism=Drosophila melanogaster, GI17737897, Length=352, Percent_Identity=29.5454545454545, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI17137530, Length=352, Percent_Identity=27.5568181818182, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI45550770, Length=352, Percent_Identity=28.125, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI45551930, Length=352, Percent_Identity=28.125, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI221457811, Length=352, Percent_Identity=28.125, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284; =1.1.1.1 [H]

Molecular weight: Translated: 41844; Mature: 41844

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTGSPASRLTGDNGIYISSHELQTGGKSLKTRAALLRTSPVGRPYAHTRPLSIETIELD
CCCCCCHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECC
PPGPDELLVKIGAAGLCHSDLSVINGDRPRPVPMVLGHEAAGTVEAVGSNVHDLAAGDRV
CCCHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCEEECCCCCCCHHHHCCCHHHHHCCCHH
VMSFMPSCGHCLPCAEGRPALCLPGAEANGKGTLLSGERRLHCQDVPVNHHLGVSGFADY
HHHHCCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCH
SVVSRQSVVKIEADLPLVEAALFGCAVLTGVGAIVNTCRVRPGESVAVVGLGGVGLAALL
HHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHH
GAVAAGAERIVAVDLAEDKLKLALSLGATDTVLAADSDAAEKIRLATRGGVDHAVEAAGS
HHHHCCCCEEEEEEECCCCEEEEEECCCCCEEEECCCCHHHHEEEHHCCCHHHHHHHCCC
AKAFELAYHITRRGGLTVSAGLAHPSSQISIPAVSLVAEERSMRGSYMGSCVPARDIPRF
CHHEEEEEEEHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHH
IALYQRGRLPVDRLLSSTGPLEEINEAFDLLDQGRVIRHVIEF
HHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MKTGSPASRLTGDNGIYISSHELQTGGKSLKTRAALLRTSPVGRPYAHTRPLSIETIELD
CCCCCCHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECC
PPGPDELLVKIGAAGLCHSDLSVINGDRPRPVPMVLGHEAAGTVEAVGSNVHDLAAGDRV
CCCHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCEEECCCCCCCHHHHCCCHHHHHCCCHH
VMSFMPSCGHCLPCAEGRPALCLPGAEANGKGTLLSGERRLHCQDVPVNHHLGVSGFADY
HHHHCCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCH
SVVSRQSVVKIEADLPLVEAALFGCAVLTGVGAIVNTCRVRPGESVAVVGLGGVGLAALL
HHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHH
GAVAAGAERIVAVDLAEDKLKLALSLGATDTVLAADSDAAEKIRLATRGGVDHAVEAAGS
HHHHCCCCEEEEEEECCCCEEEEEECCCCCEEEECCCCHHHHEEEHHCCCHHHHHHHCCC
AKAFELAYHITRRGGLTVSAGLAHPSSQISIPAVSLVAEERSMRGSYMGSCVPARDIPRF
CHHEEEEEEEHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHH
IALYQRGRLPVDRLLSSTGPLEEINEAFDLLDQGRVIRHVIEF
HHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]