Click here to switch to the map view.

The map label for this gene is yafC [H]

Identifier: 150397932

GI number: 150397932

Start: 2844864

End: 2845775

Strand: Reverse

Name: yafC [H]

Synonym: Smed_2734

Alternate gene names: 150397932

Gene position: 2845775-2844864 (Counterclockwise)

Preceding gene: 150397945

Following gene: 150397929

Centisome position: 75.25

GC content: 66.34

Gene sequence:

>912_bases
ATGTCGCGGCCATCGGTCAATCGCTCGGGTGAAATGGAAGTCTTTGCGAAAGCGGTGGAGCTCGGCGGCTTTTCGGCGGC
TGCCCGCCATTACCGGATGACGCCCTCCGCAGTCAGCAAGCTTGTGCTGCGTCTCGAAGAACGCCTCGGTGTAAGGCTGG
TCAACCGCTCGACACGCAGGCTGCAGCTCACGCCCGAAGGACATGCGTTCCATGAGCGCACGGTGCGCATCCTCGCCGAT
ATCGACGAGGCGGAGCGCTCGGCATCGGCCGGGGAGAGCCCGACCGGCCGGGTCCGGCTGAACGTCAATGCCTCGTTCGG
CACTCACGTTCTGATGCGCCTTGTGCCGGAATTCCGGGCCAGCTTTCCGGCCGTCAGCCTCGACATCGTCATGACCGATG
CCGTCGTCGACTTGATGGAAGCACGAACCGACGTTGCCATCCGCGCAGGGCCGCTGAAGAGCTCGACGCTGATTGCGCGC
AAGCTCGGGGCGACGCGTATGATGATCGTGGGGGCACCGTCCTACGTAGAACGTTACGGGATGCCGTCGTCGCTAGAGGA
TCTCGCAGGACATGAGCGACTCGGCTTCTGCTACGCCCGCGCCGTCGACGGATGGCCAATAAGGGCGGAAGGGGAAACGA
TCGTGCTGCCGGCCTCGGGCGCGGTTCAGGTGAGCGACGGCGAGGGACTGCGCCAGCTGGCGCTTGCCGGAGCCGGACTG
GCACGGCTTGCGGAATTCACGGTGCGGGAGGACGTTCGCGCCGGGCGTCTCGTGCCCGTTCTGGAAGACCTCAATCCGGG
TGATCTCGAGGAGGTCCACGCGGTCTATCTCGGCCAGGGCGGGGCGCTTCCCGCCCGCGTCCGTGCCGTCCTGGATTTCC
TCGCCCGGCATGCCCGCGTCACGCCGGGGTAG

Upstream 100 bases:

>100_bases
CAACTCCATTCTTGACAATGAACTGGTTACACGAAGATGGAGCCTCTGGCACTTTGGATATAGGCTGCTAAAAGGAATAT
TATCTGTGAGCTGAATTCAT

Downstream 100 bases:

>100_bases
CGGCGAAGATCTTCGCCGCCTTTCCCGCGTTTGAGAGGAGCCAAGCCTCAGCGCTTCGGCGCTACCAGCGCGAAGACCGC
CCCTTGAGGGTCTGTGAACT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSRPSVNRSGEMEVFAKAVELGGFSAAARHYRMTPSAVSKLVLRLEERLGVRLVNRSTRRLQLTPEGHAFHERTVRILAD
IDEAERSASAGESPTGRVRLNVNASFGTHVLMRLVPEFRASFPAVSLDIVMTDAVVDLMEARTDVAIRAGPLKSSTLIAR
KLGATRMMIVGAPSYVERYGMPSSLEDLAGHERLGFCYARAVDGWPIRAEGETIVLPASGAVQVSDGEGLRQLALAGAGL
ARLAEFTVREDVRAGRLVPVLEDLNPGDLEEVHAVYLGQGGALPARVRAVLDFLARHARVTPG

Sequences:

>Translated_303_residues
MSRPSVNRSGEMEVFAKAVELGGFSAAARHYRMTPSAVSKLVLRLEERLGVRLVNRSTRRLQLTPEGHAFHERTVRILAD
IDEAERSASAGESPTGRVRLNVNASFGTHVLMRLVPEFRASFPAVSLDIVMTDAVVDLMEARTDVAIRAGPLKSSTLIAR
KLGATRMMIVGAPSYVERYGMPSSLEDLAGHERLGFCYARAVDGWPIRAEGETIVLPASGAVQVSDGEGLRQLALAGAGL
ARLAEFTVREDVRAGRLVPVLEDLNPGDLEEVHAVYLGQGGALPARVRAVLDFLARHARVTPG
>Mature_302_residues
SRPSVNRSGEMEVFAKAVELGGFSAAARHYRMTPSAVSKLVLRLEERLGVRLVNRSTRRLQLTPEGHAFHERTVRILADI
DEAERSASAGESPTGRVRLNVNASFGTHVLMRLVPEFRASFPAVSLDIVMTDAVVDLMEARTDVAIRAGPLKSSTLIARK
LGATRMMIVGAPSYVERYGMPSSLEDLAGHERLGFCYARAVDGWPIRAEGETIVLPASGAVQVSDGEGLRQLALAGAGLA
RLAEFTVREDVRAGRLVPVLEDLNPGDLEEVHAVYLGQGGALPARVRAVLDFLARHARVTPG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=298, Percent_Identity=36.5771812080537, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=32.0819112627986, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI145693193, Length=253, Percent_Identity=30.8300395256917, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI87081978, Length=294, Percent_Identity=29.2517006802721, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1789440, Length=253, Percent_Identity=28.0632411067194, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1787128, Length=283, Percent_Identity=27.208480565371, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787589, Length=271, Percent_Identity=27.3062730627306, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788706, Length=259, Percent_Identity=24.7104247104247, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1789173, Length=253, Percent_Identity=28.0632411067194, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787806, Length=240, Percent_Identity=27.9166666666667, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788481, Length=264, Percent_Identity=23.1060606060606, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32711; Mature: 32580

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPSVNRSGEMEVFAKAVELGGFSAAARHYRMTPSAVSKLVLRLEERLGVRLVNRSTRR
CCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEECCCCEE
LQLTPEGHAFHERTVRILADIDEAERSASAGESPTGRVRLNVNASFGTHVLMRLVPEFRA
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHH
SFPAVSLDIVMTDAVVDLMEARTDVAIRAGPLKSSTLIARKLGATRMMIVGAPSYVERYG
CCCCEEEEEEHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCEEEEEECHHHHHHCC
MPSSLEDLAGHERLGFCYARAVDGWPIRAEGETIVLPASGAVQVSDGEGLRQLALAGAGL
CCCCHHHHCCCHHHHHHEEECCCCCEEEECCCEEEEECCCCEEECCCCCHHHHHHHCCHH
ARLAEFTVREDVRAGRLVPVLEDLNPGDLEEVHAVYLGQGGALPARVRAVLDFLARHARV
HHHHHHHHHHHHHCCCEEHHHHCCCCCCHHHHHEEEECCCCCCHHHHHHHHHHHHHHCCC
TPG
CCC
>Mature Secondary Structure 
SRPSVNRSGEMEVFAKAVELGGFSAAARHYRMTPSAVSKLVLRLEERLGVRLVNRSTRR
CCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCEEEECCCCEE
LQLTPEGHAFHERTVRILADIDEAERSASAGESPTGRVRLNVNASFGTHVLMRLVPEFRA
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHH
SFPAVSLDIVMTDAVVDLMEARTDVAIRAGPLKSSTLIARKLGATRMMIVGAPSYVERYG
CCCCEEEEEEHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCEEEEEECHHHHHHCC
MPSSLEDLAGHERLGFCYARAVDGWPIRAEGETIVLPASGAVQVSDGEGLRQLALAGAGL
CCCCHHHHCCCHHHHHHEEECCCCCEEEECCCEEEEECCCCEEECCCCCHHHHHHHCCHH
ARLAEFTVREDVRAGRLVPVLEDLNPGDLEEVHAVYLGQGGALPARVRAVLDFLARHARV
HHHHHHHHHHHHHCCCEEHHHHCCCCCCHHHHHEEEECCCCCCHHHHHHHHHHHHHHCCC
TPG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]