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The map label for this gene is pyk [H]

Identifier: 150397874

GI number: 150397874

Start: 2778092

End: 2779531

Strand: Reverse

Name: pyk [H]

Synonym: Smed_2676

Alternate gene names: 150397874

Gene position: 2779531-2778092 (Counterclockwise)

Preceding gene: 150397875

Following gene: 150397868

Centisome position: 73.5

GC content: 64.24

Gene sequence:

>1440_bases
ATGAGACGGAACAGGAAAGTCAAAATCCTCGCTACGCTGGGGCCGGCATCGGCCGATGAGCAGATGATCCAGAAGCTGCA
CGAGGCGGGGGCCGATCTGTTCCGTATCAATATGAGCCATGCGAGTCATGAAGTCATGCGCACTCTGATAGAGCGGATCC
GTTCGGTCGAGGCGCGTTGCGGCCGCCCCATCGGCATTCTGGCCGACCTGCAGGGTCCGAAGCTGCGCGTCGGAAAGTTC
GCGGAGGGAAAGGTCGAGCTCAAGCCCGGTCAGACCTTCACGCTCGACAATCGGGATGTGCCCGGGGACAGCCGCCGCGT
CTATCTTCCGCATCCGGAGATCCTCGAAGCCGTCAAGCCCGGCCATCGTTTGCTGATCGATGATGGCAAGCTGCATCTGC
GCGCCGAAAAAACCGATGGCAAGAGCATCGTCACCACGGTCGTCTCCGGCACGAGGATCTCTGATCGCAAGGGCGTCAGC
CTGCCCGATACGCTGCTCGGCGTCGGCGCGCTGACCGACAAGGACCGCGTCGACCTCGATGCCGTGCTGGCGACGGGGCA
GGTCGACTGGGTGGCGCTTTCCTTTATCCAGCGCCCTGAAGACCTTGCGGAGGTTCGTAAGATCGCACGGGGTCGCGTCG
GGCTGATGTCGAAAATCGAAAAGCCGCAGGCGATCGAGCGGATTGACGAGATCATCGAGCTTTCGGATGCTCTGATGGTG
GCCCGCGGCGATCTTGGCGTCGAGATGCCGCTCGAATCCGTGCCGGGGCTTCAAAAGCAATTGACGCGGGCCTGCCGGCG
AGCCGGCAAGCCCGTCGTGGTCGCAACGCAGATGCTTGAATCGATGATCTCCTCGCCGGTGCCGACGCGCGCCGAGGTGT
CGGACGTGGCCACCGCCGTGTTCGAAGGTGCCGATGCGGTGATGCTCTCGGCCGAGTCCGCTTCCGGCGAGTACCCGATC
GAGGCGGTTTCGACCATGGCGTCGATCGCCAGCAACGTCGAACGCGATCCGCATTATCCGGGGATCATCTATGCCCAGCG
CACGCCGCCGGAAGCGACGGGTGCGGATGCCATCTCGCTTGCCGCCCACCAGATCGCCGAAACGCTCAAGCTCGCAGCGA
TCGTAACCTACACATCCTCCGGCACGACCGGGCTTCGGGCGGCGCGCGAGCGGCCCCAGGTGCCGGTCATCGCGCTTTCG
CCAATCGTCCAGACGGCCCGTCGCCTTTCCGTGGTCTGGGGCCTGCACTGCGTCGTCACCGAGGATGCCACCGACCTCGA
CGACATGGTCAACCGCGCCTGTCGTATCGTCTCGACAGAAGGTTTCGGCAAGCCCGGAGACCGCGTCATCGTTACCGCCG
GTGTCCCGCTTGGTACCCCGGGGGCCACGAACATGTTGCGCATCGCCTATATCGGCTCGGACGGGCAGTCGGCGGTGTAA

Upstream 100 bases:

>100_bases
GTTTCCAGCGTATGGGATTCAAGGAATATGACACGGGGCGGCAGGGAAGCTGGATGGTTCAGCTTTCCGACACGCCCGGC
ACGCAGGAAAGCCAGAATTG

Downstream 100 bases:

>100_bases
AGCATCGCAGGATGCGTTTCGGGTCTGTGCTGCTTGTCATTTCGCCGAAGGCACGGGCGTGGTTGTAGTCGATCCCTGGC
AAGGCTGAGGTGCTGATTTC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MRRNRKVKILATLGPASADEQMIQKLHEAGADLFRINMSHASHEVMRTLIERIRSVEARCGRPIGILADLQGPKLRVGKF
AEGKVELKPGQTFTLDNRDVPGDSRRVYLPHPEILEAVKPGHRLLIDDGKLHLRAEKTDGKSIVTTVVSGTRISDRKGVS
LPDTLLGVGALTDKDRVDLDAVLATGQVDWVALSFIQRPEDLAEVRKIARGRVGLMSKIEKPQAIERIDEIIELSDALMV
ARGDLGVEMPLESVPGLQKQLTRACRRAGKPVVVATQMLESMISSPVPTRAEVSDVATAVFEGADAVMLSAESASGEYPI
EAVSTMASIASNVERDPHYPGIIYAQRTPPEATGADAISLAAHQIAETLKLAAIVTYTSSGTTGLRAARERPQVPVIALS
PIVQTARRLSVVWGLHCVVTEDATDLDDMVNRACRIVSTEGFGKPGDRVIVTAGVPLGTPGATNMLRIAYIGSDGQSAV

Sequences:

>Translated_479_residues
MRRNRKVKILATLGPASADEQMIQKLHEAGADLFRINMSHASHEVMRTLIERIRSVEARCGRPIGILADLQGPKLRVGKF
AEGKVELKPGQTFTLDNRDVPGDSRRVYLPHPEILEAVKPGHRLLIDDGKLHLRAEKTDGKSIVTTVVSGTRISDRKGVS
LPDTLLGVGALTDKDRVDLDAVLATGQVDWVALSFIQRPEDLAEVRKIARGRVGLMSKIEKPQAIERIDEIIELSDALMV
ARGDLGVEMPLESVPGLQKQLTRACRRAGKPVVVATQMLESMISSPVPTRAEVSDVATAVFEGADAVMLSAESASGEYPI
EAVSTMASIASNVERDPHYPGIIYAQRTPPEATGADAISLAAHQIAETLKLAAIVTYTSSGTTGLRAARERPQVPVIALS
PIVQTARRLSVVWGLHCVVTEDATDLDDMVNRACRIVSTEGFGKPGDRVIVTAGVPLGTPGATNMLRIAYIGSDGQSAV
>Mature_479_residues
MRRNRKVKILATLGPASADEQMIQKLHEAGADLFRINMSHASHEVMRTLIERIRSVEARCGRPIGILADLQGPKLRVGKF
AEGKVELKPGQTFTLDNRDVPGDSRRVYLPHPEILEAVKPGHRLLIDDGKLHLRAEKTDGKSIVTTVVSGTRISDRKGVS
LPDTLLGVGALTDKDRVDLDAVLATGQVDWVALSFIQRPEDLAEVRKIARGRVGLMSKIEKPQAIERIDEIIELSDALMV
ARGDLGVEMPLESVPGLQKQLTRACRRAGKPVVVATQMLESMISSPVPTRAEVSDVATAVFEGADAVMLSAESASGEYPI
EAVSTMASIASNVERDPHYPGIIYAQRTPPEATGADAISLAAHQIAETLKLAAIVTYTSSGTTGLRAARERPQVPVIALS
PIVQTARRLSVVWGLHCVVTEDATDLDDMVNRACRIVSTEGFGKPGDRVIVTAGVPLGTPGATNMLRIAYIGSDGQSAV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=487, Percent_Identity=37.1663244353183, Blast_Score=284, Evalue=1e-76,
Organism=Homo sapiens, GI33286422, Length=487, Percent_Identity=36.9609856262834, Blast_Score=282, Evalue=6e-76,
Organism=Homo sapiens, GI33286420, Length=487, Percent_Identity=36.9609856262834, Blast_Score=282, Evalue=6e-76,
Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=37.5, Blast_Score=268, Evalue=1e-71,
Organism=Homo sapiens, GI32967597, Length=496, Percent_Identity=37.5, Blast_Score=267, Evalue=2e-71,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=35.4166666666667, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=35.4166666666667, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI310128736, Length=238, Percent_Identity=30.672268907563, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI310128734, Length=238, Percent_Identity=30.672268907563, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI310128738, Length=213, Percent_Identity=30.5164319248826, Blast_Score=96, Evalue=7e-20,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=38.7840670859539, Blast_Score=294, Evalue=9e-81,
Organism=Escherichia coli, GI1787965, Length=463, Percent_Identity=36.0691144708423, Blast_Score=275, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI17544584, Length=485, Percent_Identity=35.8762886597938, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI71984413, Length=483, Percent_Identity=36.6459627329193, Blast_Score=260, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI17506829, Length=483, Percent_Identity=36.6459627329193, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI71984406, Length=483, Percent_Identity=36.6459627329193, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17506831, Length=483, Percent_Identity=36.6459627329193, Blast_Score=259, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=34.3621399176955, Blast_Score=247, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6324923, Length=491, Percent_Identity=33.8085539714868, Blast_Score=236, Evalue=4e-63,
Organism=Drosophila melanogaster, GI24648964, Length=485, Percent_Identity=36.9072164948454, Blast_Score=284, Evalue=9e-77,
Organism=Drosophila melanogaster, GI28571814, Length=491, Percent_Identity=36.2525458248472, Blast_Score=284, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24648966, Length=387, Percent_Identity=36.9509043927649, Blast_Score=239, Evalue=4e-63,
Organism=Drosophila melanogaster, GI24581235, Length=486, Percent_Identity=30.2469135802469, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24646914, Length=241, Percent_Identity=38.5892116182573, Blast_Score=162, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51459; Mature: 51459

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRNRKVKILATLGPASADEQMIQKLHEAGADLFRINMSHASHEVMRTLIERIRSVEARC
CCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHC
GRPIGILADLQGPKLRVGKFAEGKVELKPGQTFTLDNRDVPGDSRRVYLPHPEILEAVKP
CCCEEEEEECCCCCEEECCCCCCEEEECCCCEEEECCCCCCCCCCEEECCCHHHHHHCCC
GHRLLIDDGKLHLRAEKTDGKSIVTTVVSGTRISDRKGVSLPDTLLGVGALTDKDRVDLD
CCEEEEECCEEEEEEECCCCCEEEEEEECCCEECCCCCCCCCHHHHHCCCCCCCCCCCHH
AVLATGQVDWVALSFIQRPEDLAEVRKIARGRVGLMSKIEKPQAIERIDEIIELSDALMV
HHEECCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHEE
ARGDLGVEMPLESVPGLQKQLTRACRRAGKPVVVATQMLESMISSPVPTRAEVSDVATAV
EECCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHH
FEGADAVMLSAESASGEYPIEAVSTMASIASNVERDPHYPGIIYAQRTPPEATGADAISL
HCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHH
AAHQIAETLKLAAIVTYTSSGTTGLRAARERPQVPVIALSPIVQTARRLSVVWGLHCVVT
HHHHHHHHHHEEEEEEEECCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCEEEEEE
EDATDLDDMVNRACRIVSTEGFGKPGDRVIVTAGVPLGTPGATNMLRIAYIGSDGQSAV
CCCCCHHHHHHHHHEEEECCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MRRNRKVKILATLGPASADEQMIQKLHEAGADLFRINMSHASHEVMRTLIERIRSVEARC
CCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHC
GRPIGILADLQGPKLRVGKFAEGKVELKPGQTFTLDNRDVPGDSRRVYLPHPEILEAVKP
CCCEEEEEECCCCCEEECCCCCCEEEECCCCEEEECCCCCCCCCCEEECCCHHHHHHCCC
GHRLLIDDGKLHLRAEKTDGKSIVTTVVSGTRISDRKGVSLPDTLLGVGALTDKDRVDLD
CCEEEEECCEEEEEEECCCCCEEEEEEECCCEECCCCCCCCCHHHHHCCCCCCCCCCCHH
AVLATGQVDWVALSFIQRPEDLAEVRKIARGRVGLMSKIEKPQAIERIDEIIELSDALMV
HHEECCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHEE
ARGDLGVEMPLESVPGLQKQLTRACRRAGKPVVVATQMLESMISSPVPTRAEVSDVATAV
EECCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHH
FEGADAVMLSAESASGEYPIEAVSTMASIASNVERDPHYPGIIYAQRTPPEATGADAISL
HCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHH
AAHQIAETLKLAAIVTYTSSGTTGLRAARERPQVPVIALSPIVQTARRLSVVWGLHCVVT
HHHHHHHHHHEEEEEEEECCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCEEEEEE
EDATDLDDMVNRACRIVSTEGFGKPGDRVIVTAGVPLGTPGATNMLRIAYIGSDGQSAV
CCCCCHHHHHHHHHEEEECCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA