Click here to switch to the map view.

The map label for this gene is pecT [H]

Identifier: 150397845

GI number: 150397845

Start: 2750954

End: 2751934

Strand: Direct

Name: pecT [H]

Synonym: Smed_2647

Alternate gene names: 150397845

Gene position: 2750954-2751934 (Clockwise)

Preceding gene: 150397838

Following gene: 150397848

Centisome position: 72.74

GC content: 63.1

Gene sequence:

>981_bases
ATGGCTGCCATCAGGTTTTCTTATGGCGCCAGCATGGTAAATCCGCTCGACAGCGATCTCCTCAGAACCTTTCTCGCCGT
TGCCGATACCGGCAACGTGACGCGGGCTGCCGACATCGTGCGCCGCACGCAATCGGCGGTCAGCATGCAGATTCGCAAGC
TCGAGGAGCTCATCGGCTCCGCGCTGTTCGAACGCCATTCGCGCGGCGTGATACTGACGGACGAGGGCCGGCGTCTGGTC
GACAACGCACGCCGTATCGTAACCCTTCTCGACGACACGGCGGCAGCGATGCGCTCACCGGCGCTTGACGGTTCGGTCCG
GATCGGGATTTCGGAAGAGTATATCAACTCCACACTCCCGAAGGCGCTCGGTGCTTTCGCGGCCATTCATCCTGGCGTGG
AAGTGACGGTGCGGCAGGATACCTCGATGTCCAATACGGCAGCGCTCGCGGCCGGAGAAATCGACATCGCCGTCGTCTTC
GAGCCGGGCGGACGCACCCGGAGCGAGGTTCTCATGGTGAATCCTACCGTATGGGTGACCTGCGACCAGCATGGCGCCCA
CCAAAGGCAGCCGTTACCGATCGCCACCTATACATATGCGGAAGGCGGCTGGTGCGACGACCTGGCTCTGCGGAGCCTGA
AGGCGCGCGGCCTGGAGTGCCGCATCGCCTATGTGAGCCGCACGAGCGGCGGGCTCATTGCTGCCGTCCTGTCGGGCCTT
GCGATCGCACCTTTATCGCGAAGCTCCATACCGGCCGGCTGCCGCGAACTCACCGAGGAAGACGGCTTCGGTATCATCGA
CATGTCGAACGTGGTGCTGCGAACGAGGCGCGAGAACCGGTCGCCGACGGTCAACGCCATGGCGGATGCCATCCGACGCG
CCTTTCGAGCACCGGCCAGGCTGGAAGGGGCCGAGCAGGAAAAGGAAGAAGCGCGATCCACAACGACCGATGGATTTTTT
ACGATCCCGGGTACCTACTAG

Upstream 100 bases:

>100_bases
CGCGCGGGCAGGACCAGAACGGGCATGGCAAAAACCTCGGAAGGATAGTGGCGGACCTATCTTCGCGCTGCCGTTGACAT
CTGACAAATGAATTGCCACC

Downstream 100 bases:

>100_bases
GTAGGTGGGCACCGTATGTCCGGACCCACGGGTCCGGCCAGGGACAGTTCCCTTGAGATCGTGTATGGCCCCGGGGATTG
TGGTTCCTGTCGAGAAGTGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Pectinase gene transcriptional regulator [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MAAIRFSYGASMVNPLDSDLLRTFLAVADTGNVTRAADIVRRTQSAVSMQIRKLEELIGSALFERHSRGVILTDEGRRLV
DNARRIVTLLDDTAAAMRSPALDGSVRIGISEEYINSTLPKALGAFAAIHPGVEVTVRQDTSMSNTAALAAGEIDIAVVF
EPGGRTRSEVLMVNPTVWVTCDQHGAHQRQPLPIATYTYAEGGWCDDLALRSLKARGLECRIAYVSRTSGGLIAAVLSGL
AIAPLSRSSIPAGCRELTEEDGFGIIDMSNVVLRTRRENRSPTVNAMADAIRRAFRAPARLEGAEQEKEEARSTTTDGFF
TIPGTY

Sequences:

>Translated_326_residues
MAAIRFSYGASMVNPLDSDLLRTFLAVADTGNVTRAADIVRRTQSAVSMQIRKLEELIGSALFERHSRGVILTDEGRRLV
DNARRIVTLLDDTAAAMRSPALDGSVRIGISEEYINSTLPKALGAFAAIHPGVEVTVRQDTSMSNTAALAAGEIDIAVVF
EPGGRTRSEVLMVNPTVWVTCDQHGAHQRQPLPIATYTYAEGGWCDDLALRSLKARGLECRIAYVSRTSGGLIAAVLSGL
AIAPLSRSSIPAGCRELTEEDGFGIIDMSNVVLRTRRENRSPTVNAMADAIRRAFRAPARLEGAEQEKEEARSTTTDGFF
TIPGTY
>Mature_325_residues
AAIRFSYGASMVNPLDSDLLRTFLAVADTGNVTRAADIVRRTQSAVSMQIRKLEELIGSALFERHSRGVILTDEGRRLVD
NARRIVTLLDDTAAAMRSPALDGSVRIGISEEYINSTLPKALGAFAAIHPGVEVTVRQDTSMSNTAALAAGEIDIAVVFE
PGGRTRSEVLMVNPTVWVTCDQHGAHQRQPLPIATYTYAEGGWCDDLALRSLKARGLECRIAYVSRTSGGLIAAVLSGLA
IAPLSRSSIPAGCRELTEEDGFGIIDMSNVVLRTRRENRSPTVNAMADAIRRAFRAPARLEGAEQEKEEARSTTTDGFFT
IPGTY

Specific function: Regulates pectinase gene expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788626, Length=248, Percent_Identity=31.8548387096774, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1789173, Length=154, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787601, Length=143, Percent_Identity=31.4685314685315, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693105, Length=140, Percent_Identity=27.1428571428571, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI157672245, Length=141, Percent_Identity=31.9148936170213, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081904, Length=143, Percent_Identity=34.2657342657343, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI2367136, Length=146, Percent_Identity=30.8219178082192, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787879, Length=145, Percent_Identity=32.4137931034483, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI48994962, Length=149, Percent_Identity=29.5302013422819, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1790208, Length=144, Percent_Identity=31.25, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788748, Length=294, Percent_Identity=23.8095238095238, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI145693193, Length=119, Percent_Identity=31.0924369747899, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI1788297, Length=158, Percent_Identity=29.746835443038, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35195; Mature: 35064

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIRFSYGASMVNPLDSDLLRTFLAVADTGNVTRAADIVRRTQSAVSMQIRKLEELIGS
CCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALFERHSRGVILTDEGRRLVDNARRIVTLLDDTAAAMRSPALDGSVRIGISEEYINSTLP
HHHHHHCCCEEEECCCCHHHHHHHHEEEHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHH
KALGAFAAIHPGVEVTVRQDTSMSNTAALAAGEIDIAVVFEPGGRTRSEVLMVNPTVWVT
HHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCEEEEEEECCCCCCCCCEEEECCEEEEE
CDQHGAHQRQPLPIATYTYAEGGWCDDLALRSLKARGLECRIAYVSRTSGGLIAAVLSGL
ECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHCCC
AIAPLSRSSIPAGCRELTEEDGFGIIDMSNVVLRTRRENRSPTVNAMADAIRRAFRAPAR
EECCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHH
LEGAEQEKEEARSTTTDGFFTIPGTY
CCCCHHHHHHHHCCCCCCEEECCCCC
>Mature Secondary Structure 
AAIRFSYGASMVNPLDSDLLRTFLAVADTGNVTRAADIVRRTQSAVSMQIRKLEELIGS
CEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALFERHSRGVILTDEGRRLVDNARRIVTLLDDTAAAMRSPALDGSVRIGISEEYINSTLP
HHHHHHCCCEEEECCCCHHHHHHHHEEEHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHH
KALGAFAAIHPGVEVTVRQDTSMSNTAALAAGEIDIAVVFEPGGRTRSEVLMVNPTVWVT
HHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCEEEEEEECCCCCCCCCEEEECCEEEEE
CDQHGAHQRQPLPIATYTYAEGGWCDDLALRSLKARGLECRIAYVSRTSGGLIAAVLSGL
ECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHCCC
AIAPLSRSSIPAGCRELTEEDGFGIIDMSNVVLRTRRENRSPTVNAMADAIRRAFRAPAR
EECCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHH
LEGAEQEKEEARSTTTDGFFTIPGTY
CCCCHHHHHHHHCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626286 [H]