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The map label for this gene is tilS [H]

Identifier: 150397822

GI number: 150397822

Start: 2724031

End: 2725392

Strand: Reverse

Name: tilS [H]

Synonym: Smed_2624

Alternate gene names: 150397822

Gene position: 2725392-2724031 (Counterclockwise)

Preceding gene: 150397823

Following gene: 150397821

Centisome position: 72.06

GC content: 65.35

Gene sequence:

>1362_bases
ATGCCCGATGCCGTCTTCGACACGGCCAGTTATTTTCTTCGTTCCTTTACGCGGCCCTGCAGGATTCTCGTGGCCGTCTC
CGGTGGCAGCGATTCGATGGGCGTGCTCATGGCCCTGCACGCGGCGATCGCAACAGGCGAGCACCCCGGATTTTCCCTTG
CGGCCTGCACCGTCGATCACGCGCTGAGACCCCAGTCTGCCGAGGAAGCCGCCAGCGTTGCTGCCTTCTGCAGAACGCTT
GACATCGCTCACCGCACCCGCCGCTGGGAGGGCGCGAAGCCAGCGACCGGCATCCAGGCGGCGGCGCGGAAGATACGTTA
CGAATTGCTCGCCGTTGCCGCCGAGGCGCTTGGCGCCGACTGCATCGTCACGGGCCATACGCGCGAAGACCAGCACGAAA
CGATCGAAATGCGCCGCGCTCGCTCAAACGGCCCCGGTATAGACGATGGGGAGGGTGAGGGGCATGGCGGTGCGGGCATG
GCCGCAACCATGCTTTACGGGCGACGGGTCTGGGTTCTGCGGCCGTTTCTGCGTTTGGGGCGCGCCGAGATCCGAAGCTT
TCTCGACGCACGCGCCGTCGCCTGGATCGACGATCCGAGCAACGCCAACCACGCCTTCGAGCGGGTTCGGGTTCGTGCCA
GGATCGCGGCCTCAGACGATATGCAGATGCCCTTGTGGGGTGGACGGCAGCGCGCTGCTTCTTCGGCGGCAGCGGCCGGG
CTGATCGAGGATCGCATCCGCGTTCATGAGGCGCTGGTCGCGGAGATTACTGCGGAGAACGCTGGTGCAACGGACGATCC
GGACTGGCGCCGAGCGCTCCTGATCGTCGCTTCGGCTCTCGGCGGCCGCGATCATCTGCCCGCACGGGCAACGGTGCAGC
GGCTTTCGGAGTTCCTGCGGGCCGGCAAGCCTGGCCGCATGACGGCGGGCCGGGTCGTCTTCGACCGACGTGCAGGCGGA
TTGTATCTTTACCGCGAGGCCCGCAACCTGCCCGTGCTTGCCCTTGGACCGGGCGGACAGGGACTGTGGGACGGGCGCTT
CAAGATAACGAGCGGCGGACCAGCCCTCGTCGTTACCGCTGACGCATCGGGGAGACGATCGGCCCAGAGGCTTATTGACG
CAGGGCTGCCAATGGGGATTGTCAAGCGCGCATCCACGGTGGCACCAGGCATCGCGCCGGCGGATGCCGCGGGAATCGCC
TACGGCGAGGCTCTGGCCGAAATCGAGTATAACATTGCGCCTTTCGACACCTTTTTGCCGGGTTTCGACAGGATCATGGC
GGATGCGGTCGCCGTGTCTTTCGGGCGTGAACGATACCCCGCTCCGCCAGTGCATGATGTTTTGACAGAAATGGGAATGT
AA

Upstream 100 bases:

>100_bases
CGCTGCGCGAGGTCGGCAAGCGCTATCCGAAAGCATCGCCGGCCGTGAAGGCCAAGGTGACGAGCGAACAGAGCCGTTTC
GGCTGCTGAGGCGGGCCCAT

Downstream 100 bases:

>100_bases
CCGGCGGTTTTCCTTGGCAAGATCATCCCGGATACCTATGTTAGGACCAAGAATACAGCCTGGCGAATGCGCGGGTTGCG
ACAGGTTCCGGGGAGTTCGA

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MPDAVFDTASYFLRSFTRPCRILVAVSGGSDSMGVLMALHAAIATGEHPGFSLAACTVDHALRPQSAEEAASVAAFCRTL
DIAHRTRRWEGAKPATGIQAAARKIRYELLAVAAEALGADCIVTGHTREDQHETIEMRRARSNGPGIDDGEGEGHGGAGM
AATMLYGRRVWVLRPFLRLGRAEIRSFLDARAVAWIDDPSNANHAFERVRVRARIAASDDMQMPLWGGRQRAASSAAAAG
LIEDRIRVHEALVAEITAENAGATDDPDWRRALLIVASALGGRDHLPARATVQRLSEFLRAGKPGRMTAGRVVFDRRAGG
LYLYREARNLPVLALGPGGQGLWDGRFKITSGGPALVVTADASGRRSAQRLIDAGLPMGIVKRASTVAPGIAPADAAGIA
YGEALAEIEYNIAPFDTFLPGFDRIMADAVAVSFGRERYPAPPVHDVLTEMGM

Sequences:

>Translated_453_residues
MPDAVFDTASYFLRSFTRPCRILVAVSGGSDSMGVLMALHAAIATGEHPGFSLAACTVDHALRPQSAEEAASVAAFCRTL
DIAHRTRRWEGAKPATGIQAAARKIRYELLAVAAEALGADCIVTGHTREDQHETIEMRRARSNGPGIDDGEGEGHGGAGM
AATMLYGRRVWVLRPFLRLGRAEIRSFLDARAVAWIDDPSNANHAFERVRVRARIAASDDMQMPLWGGRQRAASSAAAAG
LIEDRIRVHEALVAEITAENAGATDDPDWRRALLIVASALGGRDHLPARATVQRLSEFLRAGKPGRMTAGRVVFDRRAGG
LYLYREARNLPVLALGPGGQGLWDGRFKITSGGPALVVTADASGRRSAQRLIDAGLPMGIVKRASTVAPGIAPADAAGIA
YGEALAEIEYNIAPFDTFLPGFDRIMADAVAVSFGRERYPAPPVHDVLTEMGM
>Mature_452_residues
PDAVFDTASYFLRSFTRPCRILVAVSGGSDSMGVLMALHAAIATGEHPGFSLAACTVDHALRPQSAEEAASVAAFCRTLD
IAHRTRRWEGAKPATGIQAAARKIRYELLAVAAEALGADCIVTGHTREDQHETIEMRRARSNGPGIDDGEGEGHGGAGMA
ATMLYGRRVWVLRPFLRLGRAEIRSFLDARAVAWIDDPSNANHAFERVRVRARIAASDDMQMPLWGGRQRAASSAAAAGL
IEDRIRVHEALVAEITAENAGATDDPDWRRALLIVASALGGRDHLPARATVQRLSEFLRAGKPGRMTAGRVVFDRRAGGL
YLYREARNLPVLALGPGGQGLWDGRFKITSGGPALVVTADASGRRSAQRLIDAGLPMGIVKRASTVAPGIAPADAAGIAY
GEALAEIEYNIAPFDTFLPGFDRIMADAVAVSFGRERYPAPPVHDVLTEMGM

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=205, Percent_Identity=29.2682926829268, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 48377; Mature: 48245

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDAVFDTASYFLRSFTRPCRILVAVSGGSDSMGVLMALHAAIATGEHPGFSLAACTVDH
CCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHH
ALRPQSAEEAASVAAFCRTLDIAHRTRRWEGAKPATGIQAAARKIRYELLAVAAEALGAD
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
CIVTGHTREDQHETIEMRRARSNGPGIDDGEGEGHGGAGMAATMLYGRRVWVLRPFLRLG
EEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHC
RAEIRSFLDARAVAWIDDPSNANHAFERVRVRARIAASDDMQMPLWGGRQRAASSAAAAG
HHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHH
LIEDRIRVHEALVAEITAENAGATDDPDWRRALLIVASALGGRDHLPARATVQRLSEFLR
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
AGKPGRMTAGRVVFDRRAGGLYLYREARNLPVLALGPGGQGLWDGRFKITSGGPALVVTA
CCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEECCCCCCCCCCEEEEECCCCEEEEEE
DASGRRSAQRLIDAGLPMGIVKRASTVAPGIAPADAAGIAYGEALAEIEYNIAPFDTFLP
CCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCC
GFDRIMADAVAVSFGRERYPAPPVHDVLTEMGM
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
PDAVFDTASYFLRSFTRPCRILVAVSGGSDSMGVLMALHAAIATGEHPGFSLAACTVDH
CCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHH
ALRPQSAEEAASVAAFCRTLDIAHRTRRWEGAKPATGIQAAARKIRYELLAVAAEALGAD
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
CIVTGHTREDQHETIEMRRARSNGPGIDDGEGEGHGGAGMAATMLYGRRVWVLRPFLRLG
EEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHC
RAEIRSFLDARAVAWIDDPSNANHAFERVRVRARIAASDDMQMPLWGGRQRAASSAAAAG
HHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHH
LIEDRIRVHEALVAEITAENAGATDDPDWRRALLIVASALGGRDHLPARATVQRLSEFLR
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
AGKPGRMTAGRVVFDRRAGGLYLYREARNLPVLALGPGGQGLWDGRFKITSGGPALVVTA
CCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEECCCCCCCCCCEEEEECCCCEEEEEE
DASGRRSAQRLIDAGLPMGIVKRASTVAPGIAPADAAGIAYGEALAEIEYNIAPFDTFLP
CCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCC
GFDRIMADAVAVSFGRERYPAPPVHDVLTEMGM
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]