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The map label for this gene is yddQ [H]

Identifier: 150397796

GI number: 150397796

Start: 2695030

End: 2695575

Strand: Reverse

Name: yddQ [H]

Synonym: Smed_2598

Alternate gene names: 150397796

Gene position: 2695575-2695030 (Counterclockwise)

Preceding gene: 150397798

Following gene: 150397795

Centisome position: 71.28

GC content: 58.42

Gene sequence:

>546_bases
ATGAGCAAGCGCGCGGTAATCGTTGTCGATCTTCAGAAAGACTACCTGGCTTCAGGAAAATTCGCTTTGGTCGGTATGGA
CGCTGCGATCGAAAACGCTGCAAGGATCGTGGCCATGGCTCGTCGTTCCGGCGATCTGGTGATCAACGTTCGACACGAGG
GACCCACCGATGCCCCCTTCTTCGTCTCGGGCACTGAGGGGGCGGAGATCGTGCCTGCCATGACCCCGCAGGAGGGCGAG
GACGTCATAACCAAGAACTACCCCAATGCGTTCCGTGAAACGAATTTGGCGGACTTGCTGTCGTCTGCTGCGATTGAAGA
CGTGACGATCATCGGGGCCATGAGTCACATGTGCATCGATGCGACCGCAAGGGCTGCTGCCGATCTCGGGTACAAGACGA
CCATCATCCAGGATGCTTGCGCTACACGCGATCTTGAATACCGGGGCGAGGCTGTGCCCGCCGCGAAGGTCCACGCTGCC
TTCATGTCTGCGCTCGCCTTCGGATACGGGAAGGTGCTTTCGACCGACGAATATCTGGCGAAGTGA

Upstream 100 bases:

>100_bases
CTTCGTTTTGTCCATATCACCCTGAAGTTTGTCACTCACGACACTCATACGATGCCTTAGCCCTCATTATACAGGCGCAT
CAACTTCGGAAAGGATGCTC

Downstream 100 bases:

>100_bases
TCCCCACCCGACGACCGCAACAGCAAGAGAAAGCCATGACAGATCCAAGTCGGAATCCAATGCTCGTAGGCCGCCGTGGC
ACGCTTAAACTGTTCGCCGC

Product: isochorismatase hydrolase

Products: Nicotinate; pyrazinoic acid; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MSKRAVIVVDLQKDYLASGKFALVGMDAAIENAARIVAMARRSGDLVINVRHEGPTDAPFFVSGTEGAEIVPAMTPQEGE
DVITKNYPNAFRETNLADLLSSAAIEDVTIIGAMSHMCIDATARAAADLGYKTTIIQDACATRDLEYRGEAVPAAKVHAA
FMSALAFGYGKVLSTDEYLAK

Sequences:

>Translated_181_residues
MSKRAVIVVDLQKDYLASGKFALVGMDAAIENAARIVAMARRSGDLVINVRHEGPTDAPFFVSGTEGAEIVPAMTPQEGE
DVITKNYPNAFRETNLADLLSSAAIEDVTIIGAMSHMCIDATARAAADLGYKTTIIQDACATRDLEYRGEAVPAAKVHAA
FMSALAFGYGKVLSTDEYLAK
>Mature_180_residues
SKRAVIVVDLQKDYLASGKFALVGMDAAIENAARIVAMARRSGDLVINVRHEGPTDAPFFVSGTEGAEIVPAMTPQEGED
VITKNYPNAFRETNLADLLSSAAIEDVTIIGAMSHMCIDATARAAADLGYKTTIIQDACATRDLEYRGEAVPAAKVHAAF
MSALAFGYGKVLSTDEYLAK

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 19260; Mature: 19129

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRAVIVVDLQKDYLASGKFALVGMDAAIENAARIVAMARRSGDLVINVRHEGPTDAPF
CCCCEEEEEECCHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCE
FVSGTEGAEIVPAMTPQEGEDVITKNYPNAFRETNLADLLSSAAIEDVTIIGAMSHMCID
EEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ATARAAADLGYKTTIIQDACATRDLEYRGEAVPAAKVHAAFMSALAFGYGKVLSTDEYLA
HHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHC
K
C
>Mature Secondary Structure 
SKRAVIVVDLQKDYLASGKFALVGMDAAIENAARIVAMARRSGDLVINVRHEGPTDAPF
CCCEEEEEECCHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCE
FVSGTEGAEIVPAMTPQEGEDVITKNYPNAFRETNLADLLSSAAIEDVTIIGAMSHMCID
EEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ATARAAADLGYKTTIIQDACATRDLEYRGEAVPAAKVHAAFMSALAFGYGKVLSTDEYLA
HHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Nicotinamide; pyrazinamide; H2O [C]

Specific reaction: Nicotinamide + H2O = Nicotinate + NH3 pyrazinamide + H2O = pyrazinoic acid + NH3 [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]