Click here to switch to the map view.

The map label for this gene is opuAB [H]

Identifier: 150397782

GI number: 150397782

Start: 2685277

End: 2686134

Strand: Direct

Name: opuAB [H]

Synonym: Smed_2584

Alternate gene names: 150397782

Gene position: 2685277-2686134 (Clockwise)

Preceding gene: 150397781

Following gene: 150397783

Centisome position: 71.0

GC content: 63.64

Gene sequence:

>858_bases
ATGTTTCCGGACTCTCTCAATCTCTCGATCCGCGCGCCGGTGAACGACTTCATCCAGGCGCTCGTCGCCAATTACGGCTG
GGTGTTCAAGGCGATCAGCGGTGTCATCCTGAAGGCCGTGCTGTTCATCGAATGGATCCTGCGCGGCCTGCCCTGGTGGC
TTGTTATCCTCGCCTTCATGGCGCTCGCCTGGCGGAGTTCGCGGCGGTGGTCGCTGACACTGGCCGTCGGCGCCTTGCTC
CTTGCCGTCGGCGTCCTCGGTCTCTGGGACCTGACGATGCAAACGCTCGCGCTGATGCTGATGGCGACAATCGTCTCGGT
CGTGATCGGCGTACCCATGGGCATTCTCGTCGCCAAGAGCCGCGTCGTCCGCAACATCACCCTGCCGGTGCTCGACGTGA
TGCAGACCATGCCGAGCTTCGTGTATCTGATCCCGGCTCTGATGCTCTTCGGGCTGGGCAAGGTGCCGGCGATCCTCGCG
ACCATCATCTACGCCGTTCCGCCGCTGATCCGGCTGACCGATCTCGGCATCCGCCAGGTCGACGCGGAAGTCGTCGAGGC
TGCAACGGCGTTCGGCGGCAGCCCGGGGCAGATTCTCTTCGGCGTCGAAATGCCGCTTGCGACACCGACCATCATGGCGG
GCCTCAACCAGACGATCATGATGGCGCTTTCGATGGTGGTCGTTGCCTCGATGATCGGCGCACGCGGCCTCGGCGAACAG
GTATTGAACGGCATCCAGACGCTCGATGTCGGCAAGGGCCTGGAAGCCGGTATCGGCATCGTCATTCTCGCAATCGTGCT
CGACCGCATCACGCAGGGCTTCGGCAAGGTCCGGACGGAGGACGCCCGCAATGGCTGA

Upstream 100 bases:

>100_bases
CTGCCCCACGCGGGGTAAGGTGATCGTAGGCAGAAGGCTGTTTCCCCGGCCAGGGGGGCTTTCGGCTTGCCGTCGCCCAC
CTGAACAAGGAACCTACCCG

Downstream 100 bases:

>100_bases
TATTGAGATCCGGAACGTCTACAAGATTTTCGGGCACGACGCGAAGAAGGCCCTGGGCATGGTCAGGGATGGCCTCGGCA
AGGCAGATATCCTGGCGAAA

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MFPDSLNLSIRAPVNDFIQALVANYGWVFKAISGVILKAVLFIEWILRGLPWWLVILAFMALAWRSSRRWSLTLAVGALL
LAVGVLGLWDLTMQTLALMLMATIVSVVIGVPMGILVAKSRVVRNITLPVLDVMQTMPSFVYLIPALMLFGLGKVPAILA
TIIYAVPPLIRLTDLGIRQVDAEVVEAATAFGGSPGQILFGVEMPLATPTIMAGLNQTIMMALSMVVVASMIGARGLGEQ
VLNGIQTLDVGKGLEAGIGIVILAIVLDRITQGFGKVRTEDARNG

Sequences:

>Translated_285_residues
MFPDSLNLSIRAPVNDFIQALVANYGWVFKAISGVILKAVLFIEWILRGLPWWLVILAFMALAWRSSRRWSLTLAVGALL
LAVGVLGLWDLTMQTLALMLMATIVSVVIGVPMGILVAKSRVVRNITLPVLDVMQTMPSFVYLIPALMLFGLGKVPAILA
TIIYAVPPLIRLTDLGIRQVDAEVVEAATAFGGSPGQILFGVEMPLATPTIMAGLNQTIMMALSMVVVASMIGARGLGEQ
VLNGIQTLDVGKGLEAGIGIVILAIVLDRITQGFGKVRTEDARNG
>Mature_285_residues
MFPDSLNLSIRAPVNDFIQALVANYGWVFKAISGVILKAVLFIEWILRGLPWWLVILAFMALAWRSSRRWSLTLAVGALL
LAVGVLGLWDLTMQTLALMLMATIVSVVIGVPMGILVAKSRVVRNITLPVLDVMQTMPSFVYLIPALMLFGLGKVPAILA
TIIYAVPPLIRLTDLGIRQVDAEVVEAATAFGGSPGQILFGVEMPLATPTIMAGLNQTIMMALSMVVVASMIGARGLGEQ
VLNGIQTLDVGKGLEAGIGIVILAIVLDRITQGFGKVRTEDARNG

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=273, Percent_Identity=47.6190476190476, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1788451, Length=211, Percent_Identity=31.2796208530806, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1788449, Length=185, Percent_Identity=29.7297297297297, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1786564, Length=152, Percent_Identity=30.9210526315789, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30518; Mature: 30518

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPDSLNLSIRAPVNDFIQALVANYGWVFKAISGVILKAVLFIEWILRGLPWWLVILAFM
CCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ALAWRSSRRWSLTLAVGALLLAVGVLGLWDLTMQTLALMLMATIVSVVIGVPMGILVAKS
HHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
RVVRNITLPVLDVMQTMPSFVYLIPALMLFGLGKVPAILATIIYAVPPLIRLTDLGIRQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
DAEVVEAATAFGGSPGQILFGVEMPLATPTIMAGLNQTIMMALSMVVVASMIGARGLGEQ
HHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VLNGIQTLDVGKGLEAGIGIVILAIVLDRITQGFGKVRTEDARNG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MFPDSLNLSIRAPVNDFIQALVANYGWVFKAISGVILKAVLFIEWILRGLPWWLVILAFM
CCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ALAWRSSRRWSLTLAVGALLLAVGVLGLWDLTMQTLALMLMATIVSVVIGVPMGILVAKS
HHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
RVVRNITLPVLDVMQTMPSFVYLIPALMLFGLGKVPAILATIIYAVPPLIRLTDLGIRQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
DAEVVEAATAFGGSPGQILFGVEMPLATPTIMAGLNQTIMMALSMVVVASMIGARGLGEQ
HHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VLNGIQTLDVGKGLEAGIGIVILAIVLDRITQGFGKVRTEDARNG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]