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The map label for this gene is proX [H]

Identifier: 150397781

GI number: 150397781

Start: 2684111

End: 2685145

Strand: Direct

Name: proX [H]

Synonym: Smed_2583

Alternate gene names: 150397781

Gene position: 2684111-2685145 (Clockwise)

Preceding gene: 150397780

Following gene: 150397782

Centisome position: 70.97

GC content: 59.03

Gene sequence:

>1035_bases
ATGTCGATCAGCACAATGAAACTCACCGTCGCGGCAGCCGGCCTGATGCTGGCGGCATCCGCCGGCGGCGCCAATGCTTC
CTATTGCGGCGATGGCAAGACCGTAACCTTCGCCGGCATAGATTGGGAAAGCGGCGCCTTCATTACCGAGGTCATGAAAA
CGATCCTTTCCAAGGGATACGACTGCCAGGTCGACTCGATTCCCGGCAATTCCGTAACTCTCGAGCAGGCAACGGCCAAT
AACGACGTTCAGATTTTCGCCGAGGAATGGCTCGGCCGTTCGGACATCTGGAACAAGGCGGTGGAAGAGAAGAAGGTGGT
TGCCGTCGGCAAGACCTTTGTAGGCGCCAGCGAAGGCTGGTTCGTGCCGGACTATGTCGTCAAGGGCGATCCCGCTCGCA
ATATCGAACCCAAGGCGCCGGACCTGAAAAGCGTATCCCAACTTGCCGACCCGAAAATCGCCGAGATCTTCGCTGATCCG
GAGGAACCGTCCAAGGGCCGCTTCCTGAACTGCCCTTCCGGCTGGACCTGCGAGGGCGTGAGCACGGCCAAGCTCGAGGC
CTACAAGCTTGGCGAGTCCTACGTGAACTTCCGCCCGGGGACGGGAACGGCGCTCGATGCGGCGATTACCTCTGCCTATC
TCCAGGGAGAGCCGATCCTCTTCTATTATTGGTCGCCGACCGCGATCATGGGTAAATACAAGCTGATCCAGCTCGAAGAG
CCGCCCTATAACGAAGCCTGCTGGAAAGAACTGAGCAGTGCCAACGGCAAGCGTGACGAAGGCTGCGCCTTTCCCTCCGT
CGACGTCTCTTATGGCGTGAACAGCACCTTCGCTTCCGAGGCGCCGGAAATCATCGAGATCCTGGAAAAGGCAACTTTTC
CGCTCGAGGAGGTCAATGCCAGCCTCGCCTATATGACGGATAATAAGGTCGATGCGACGGCAGCCGCCGCGCAGTTCCTG
AAAGCCAAGGGCGACATCTGGGGCAAGTGGGTCTCGGACGAGGCACGCGGCAAAATCGAAGCTGGCCTCGAATAA

Upstream 100 bases:

>100_bases
ACGGCATGACCGGGAGGAAGATATCGACCGCGCCTTTGCGCGCGTTCTGAACAAGCTCAAATAGCAATCGAAATCGCGAT
CAGAAAAAAGGGGAATGCGC

Downstream 100 bases:

>100_bases
CGGCAGTCTTTCGTCCGCGATCACCAGTGCCCTGCCCCACGCGGGGTAAGGTGATCGTAGGCAGAAGGCTGTTTCCCCGG
CCAGGGGGGCTTTCGGCTTG

Product: glycine betaine ABC transporter substrate-binding protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSISTMKLTVAAAGLMLAASAGGANASYCGDGKTVTFAGIDWESGAFITEVMKTILSKGYDCQVDSIPGNSVTLEQATAN
NDVQIFAEEWLGRSDIWNKAVEEKKVVAVGKTFVGASEGWFVPDYVVKGDPARNIEPKAPDLKSVSQLADPKIAEIFADP
EEPSKGRFLNCPSGWTCEGVSTAKLEAYKLGESYVNFRPGTGTALDAAITSAYLQGEPILFYYWSPTAIMGKYKLIQLEE
PPYNEACWKELSSANGKRDEGCAFPSVDVSYGVNSTFASEAPEIIEILEKATFPLEEVNASLAYMTDNKVDATAAAAQFL
KAKGDIWGKWVSDEARGKIEAGLE

Sequences:

>Translated_344_residues
MSISTMKLTVAAAGLMLAASAGGANASYCGDGKTVTFAGIDWESGAFITEVMKTILSKGYDCQVDSIPGNSVTLEQATAN
NDVQIFAEEWLGRSDIWNKAVEEKKVVAVGKTFVGASEGWFVPDYVVKGDPARNIEPKAPDLKSVSQLADPKIAEIFADP
EEPSKGRFLNCPSGWTCEGVSTAKLEAYKLGESYVNFRPGTGTALDAAITSAYLQGEPILFYYWSPTAIMGKYKLIQLEE
PPYNEACWKELSSANGKRDEGCAFPSVDVSYGVNSTFASEAPEIIEILEKATFPLEEVNASLAYMTDNKVDATAAAAQFL
KAKGDIWGKWVSDEARGKIEAGLE
>Mature_343_residues
SISTMKLTVAAAGLMLAASAGGANASYCGDGKTVTFAGIDWESGAFITEVMKTILSKGYDCQVDSIPGNSVTLEQATANN
DVQIFAEEWLGRSDIWNKAVEEKKVVAVGKTFVGASEGWFVPDYVVKGDPARNIEPKAPDLKSVSQLADPKIAEIFADPE
EPSKGRFLNCPSGWTCEGVSTAKLEAYKLGESYVNFRPGTGTALDAAITSAYLQGEPILFYYWSPTAIMGKYKLIQLEEP
PYNEACWKELSSANGKRDEGCAFPSVDVSYGVNSTFASEAPEIIEILEKATFPLEEVNASLAYMTDNKVDATAAAAQFLK
AKGDIWGKWVSDEARGKIEAGLE

Specific function: Member of a multicomponent binding-protein-dependent transport system (the proU transporter) which serves as the glycine betaine/L-proline transporter [H]

COG id: COG2113

COG function: function code E; ABC-type proline/glycine betaine transport systems, periplasmic components

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789034, Length=272, Percent_Identity=25.3676470588235, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007210 [H]

Pfam domain/function: PF04069 OpuAC [H]

EC number: NA

Molecular weight: Translated: 36976; Mature: 36845

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISTMKLTVAAAGLMLAASAGGANASYCGDGKTVTFAGIDWESGAFITEVMKTILSKGY
CCCCEEEHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCC
DCQVDSIPGNSVTLEQATANNDVQIFAEEWLGRSDIWNKAVEEKKVVAVGKTFVGASEGW
CEEEECCCCCEEEEEECCCCCCEEEEEHHHCCHHHHHHHHHHHCEEEEECCCEECCCCCC
FVPDYVVKGDPARNIEPKAPDLKSVSQLADPKIAEIFADPEEPSKGRFLNCPSGWTCEGV
CCCCEEECCCCCCCCCCCCCCHHHHHHHCCCCHHHHCCCCCCCCCCCEEECCCCCEECCC
STAKLEAYKLGESYVNFRPGTGTALDAAITSAYLQGEPILFYYWSPTAIMGKYKLIQLEE
CHHEEEEEECCCHHEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCEEECCEEEEEECC
PPYNEACWKELSSANGKRDEGCAFPSVDVSYGVNSTFASEAPEIIEILEKATFPLEEVNA
CCCCHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCHHHCCHHHHHHHHHCCCCHHHHCC
SLAYMTDNKVDATAAAAQFLKAKGDIWGKWVSDEARGKIEAGLE
EEEEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCEECCCC
>Mature Secondary Structure 
SISTMKLTVAAAGLMLAASAGGANASYCGDGKTVTFAGIDWESGAFITEVMKTILSKGY
CCCEEEHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCC
DCQVDSIPGNSVTLEQATANNDVQIFAEEWLGRSDIWNKAVEEKKVVAVGKTFVGASEGW
CEEEECCCCCEEEEEECCCCCCEEEEEHHHCCHHHHHHHHHHHCEEEEECCCEECCCCCC
FVPDYVVKGDPARNIEPKAPDLKSVSQLADPKIAEIFADPEEPSKGRFLNCPSGWTCEGV
CCCCEEECCCCCCCCCCCCCCHHHHHHHCCCCHHHHCCCCCCCCCCCEEECCCCCEECCC
STAKLEAYKLGESYVNFRPGTGTALDAAITSAYLQGEPILFYYWSPTAIMGKYKLIQLEE
CHHEEEEEECCCHHEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCEEECCEEEEEECC
PPYNEACWKELSSANGKRDEGCAFPSVDVSYGVNSTFASEAPEIIEILEKATFPLEEVNA
CCCCHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCHHHCCHHHHHHHHHCCCCHHHHCC
SLAYMTDNKVDATAAAAQFLKAKGDIWGKWVSDEARGKIEAGLE
EEEEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]