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The map label for this gene is lysC [H]

Identifier: 150397702

GI number: 150397702

Start: 2601055

End: 2602329

Strand: Reverse

Name: lysC [H]

Synonym: Smed_2504

Alternate gene names: 150397702

Gene position: 2602329-2601055 (Counterclockwise)

Preceding gene: 150397705

Following gene: 150397701

Centisome position: 68.81

GC content: 61.1

Gene sequence:

>1275_bases
ATGGCACGCATCGTGATGAAATTCGGCGGGACCTCCGTCGCAGATTTGAACCGCATCCACAACGTCGCGCGCCATGTGAA
ACGCGAAGTGGATGCCGGCCACGAAGTTGCTGTCGTCGTGTCCGCGATGTCCGGCAAGACCAACGAGCTGGTCGGGTGGG
TCGAGAACATGCCCAAGGTCGCGGGCTCGAACGCCCCCTTCTACGATGCGCGCGAATATGACGCGGTGGTCGCTTCAGGC
GAACAGGTGACCTCCGGCCTGCTTGCGATCGCCTTGCAGTCGATGGGCATCAACGCCCGCTCCTGGCAGGGCTGGCAACT
GCCGATCAGGACCGATAACGCGCATGGTGCGGCACGGATACTCGATATCGACGGTACCGATATCACCCGGCGCATGGGTG
AGGGCCAGGTGGCGGTCGTCGCCGGCTTCCAGGGTCTCGGCCCGGACAATCGCCTGGCCACGCTCGGCCGCGGCGGCTCC
GACACGTCCGCCGTCGCGATCGCAGCCGCGGTCAAGGCCGATCGCTGCGACATCTATACCGACGTGGACGGCGTCTACAC
GACGGACCCGCGCATCGAGCCGAAGGCCCGGCGATTGAAGAAAATCGCCTTTGAGGAAATGCTGGAAATGGCATCCCTCG
GTGCCAAGGTGCTGCAGGTACGCTCGGTCGAGCTCGCCATGGTGCACAAGGTGCGTACATTCGTGCGCTCGTCTTTCGAG
GACCCCGATGCGCCGGGCATGGGTGACCTCATGAACCCGCCCGGAACGCTGATATGTGATGAGGATGAGATCGTGGAACA
GGAAGTGGTAACCGGCATTGCCTATGCCAAGGATGAAGCACAGATCTCGCTGCGCCGCCTCGCCGACCGGCCGGGTGTTT
CGGCAGCGATCTTCGGACCGCTTGCCGAAGCTCATATCAATGTCGATATGATCGTCCAGAACATTTCGGAAGACGGCTCG
AAGACCGACATGACCTTCACCGTACCTTCCGGCGATGTCGACAAGGCGCTGAGGGTTCTCGGCGACAACAAGGAAAAGAT
CGGCTATGACGTGATTCAGTCGGAGTCGGGTCTCGTCAAGGTTTCGGTTATCGGCATCGGAATGCGCAGCCATGCGGGCG
TTGCCGCATCCGCGTTCCGGGCGCTCGCCGACAAGGGCATCAACATCAAGGCGATCACCACGTCCGAGATCAAGATTTCC
ATCCTGATCGACGGACCTTACGCCGAATTGGCCGTTCGCACTTTGCATTCTACCTACGGTCTGGATAAGAGTTAA

Upstream 100 bases:

>100_bases
GCCGATATCCGAGAAGTCTCCTCAAAGCTTTTTGATCGTCGGGCGCCGGCGGGGGCGTTTTCCTGTCGCGCGCAGAATAT
GTTGGTACGGTAGAGGCGAA

Downstream 100 bases:

>100_bases
ACGGGCAGGCATTTGATTTGGGAAACGCGGCCCGCGGCGCCACGTGTCTGAACAGGCGCGCAGACGTCGCTGTAATGACT
AGAACTGCTGCATGACGTGT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MARIVMKFGGTSVADLNRIHNVARHVKREVDAGHEVAVVVSAMSGKTNELVGWVENMPKVAGSNAPFYDAREYDAVVASG
EQVTSGLLAIALQSMGINARSWQGWQLPIRTDNAHGAARILDIDGTDITRRMGEGQVAVVAGFQGLGPDNRLATLGRGGS
DTSAVAIAAAVKADRCDIYTDVDGVYTTDPRIEPKARRLKKIAFEEMLEMASLGAKVLQVRSVELAMVHKVRTFVRSSFE
DPDAPGMGDLMNPPGTLICDEDEIVEQEVVTGIAYAKDEAQISLRRLADRPGVSAAIFGPLAEAHINVDMIVQNISEDGS
KTDMTFTVPSGDVDKALRVLGDNKEKIGYDVIQSESGLVKVSVIGIGMRSHAGVAASAFRALADKGINIKAITTSEIKIS
ILIDGPYAELAVRTLHSTYGLDKS

Sequences:

>Translated_424_residues
MARIVMKFGGTSVADLNRIHNVARHVKREVDAGHEVAVVVSAMSGKTNELVGWVENMPKVAGSNAPFYDAREYDAVVASG
EQVTSGLLAIALQSMGINARSWQGWQLPIRTDNAHGAARILDIDGTDITRRMGEGQVAVVAGFQGLGPDNRLATLGRGGS
DTSAVAIAAAVKADRCDIYTDVDGVYTTDPRIEPKARRLKKIAFEEMLEMASLGAKVLQVRSVELAMVHKVRTFVRSSFE
DPDAPGMGDLMNPPGTLICDEDEIVEQEVVTGIAYAKDEAQISLRRLADRPGVSAAIFGPLAEAHINVDMIVQNISEDGS
KTDMTFTVPSGDVDKALRVLGDNKEKIGYDVIQSESGLVKVSVIGIGMRSHAGVAASAFRALADKGINIKAITTSEIKIS
ILIDGPYAELAVRTLHSTYGLDKS
>Mature_423_residues
ARIVMKFGGTSVADLNRIHNVARHVKREVDAGHEVAVVVSAMSGKTNELVGWVENMPKVAGSNAPFYDAREYDAVVASGE
QVTSGLLAIALQSMGINARSWQGWQLPIRTDNAHGAARILDIDGTDITRRMGEGQVAVVAGFQGLGPDNRLATLGRGGSD
TSAVAIAAAVKADRCDIYTDVDGVYTTDPRIEPKARRLKKIAFEEMLEMASLGAKVLQVRSVELAMVHKVRTFVRSSFED
PDAPGMGDLMNPPGTLICDEDEIVEQEVVTGIAYAKDEAQISLRRLADRPGVSAAIFGPLAEAHINVDMIVQNISEDGSK
TDMTFTVPSGDVDKALRVLGDNKEKIGYDVIQSESGLVKVSVIGIGMRSHAGVAASAFRALADKGINIKAITTSEIKISI
LIDGPYAELAVRTLHSTYGLDKS

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=478, Percent_Identity=29.9163179916318, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1790455, Length=465, Percent_Identity=29.247311827957, Blast_Score=125, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6320893, Length=296, Percent_Identity=29.0540540540541, Blast_Score=97, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 45285; Mature: 45154

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIVMKFGGTSVADLNRIHNVARHVKREVDAGHEVAVVVSAMSGKTNELVGWVENMPKV
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHCCHH
AGSNAPFYDAREYDAVVASGEQVTSGLLAIALQSMGINARSWQGWQLPIRTDNAHGAARI
CCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEEE
LDIDGTDITRRMGEGQVAVVAGFQGLGPDNRLATLGRGGSDTSAVAIAAAVKADRCDIYT
EECCCCHHHHHCCCCCEEEEECCCCCCCCCCEEEECCCCCCCHHEEEEEHHCCCCCCEEE
DVDGVYTTDPRIEPKARRLKKIAFEEMLEMASLGAKVLQVRSVELAMVHKVRTFVRSSFE
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DPDAPGMGDLMNPPGTLICDEDEIVEQEVVTGIAYAKDEAQISLRRLADRPGVSAAIFGP
CCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEHHCC
LAEAHINVDMIVQNISEDGSKTDMTFTVPSGDVDKALRVLGDNKEKIGYDVIQSESGLVK
HHHHHCCHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCCHHCCHHHEECCCCEEE
VSVIGIGMRSHAGVAASAFRALADKGINIKAITTSEIKISILIDGPYAELAVRTLHSTYG
EEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCEEEEEEECCCHHHHHHHHHHHHCC
LDKS
CCCC
>Mature Secondary Structure 
ARIVMKFGGTSVADLNRIHNVARHVKREVDAGHEVAVVVSAMSGKTNELVGWVENMPKV
CEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHCCHH
AGSNAPFYDAREYDAVVASGEQVTSGLLAIALQSMGINARSWQGWQLPIRTDNAHGAARI
CCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEEE
LDIDGTDITRRMGEGQVAVVAGFQGLGPDNRLATLGRGGSDTSAVAIAAAVKADRCDIYT
EECCCCHHHHHCCCCCEEEEECCCCCCCCCCEEEECCCCCCCHHEEEEEHHCCCCCCEEE
DVDGVYTTDPRIEPKARRLKKIAFEEMLEMASLGAKVLQVRSVELAMVHKVRTFVRSSFE
CCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DPDAPGMGDLMNPPGTLICDEDEIVEQEVVTGIAYAKDEAQISLRRLADRPGVSAAIFGP
CCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEHHCC
LAEAHINVDMIVQNISEDGSKTDMTFTVPSGDVDKALRVLGDNKEKIGYDVIQSESGLVK
HHHHHCCHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHCCCCHHCCHHHEECCCCEEE
VSVIGIGMRSHAGVAASAFRALADKGINIKAITTSEIKISILIDGPYAELAVRTLHSTYG
EEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCEEEEEEECCCHHHHHHHHHHHHCC
LDKS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]