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The map label for this gene is ptsP [H]

Identifier: 150397701

GI number: 150397701

Start: 2598598

End: 2600865

Strand: Reverse

Name: ptsP [H]

Synonym: Smed_2503

Alternate gene names: 150397701

Gene position: 2600865-2598598 (Counterclockwise)

Preceding gene: 150397702

Following gene: 150397700

Centisome position: 68.77

GC content: 62.96

Gene sequence:

>2268_bases
ATGAGAGACCTTTCCGCAGGTCCGCGCGTTCTCCTCAAGCGGTTGCGCGAGCTCATGGCGGAACCGCTCGAGCCGCAAGA
GCGCCTTGACCGGATCGTGCGCCAGATAGCGCAGAACATGGTCGCGGAAGTGTGCTCGGTTTACGTGCTGCGCTCTGATG
GCGTGCTGGAACTCTACGCGACCGAGGGCCTGAACAAGACGGCGGTGCACCTGGCCCAGTTGAAGATGGGGCAAGGTCTC
GTCGGCACGATCGCCGCTTCGGCGCGGCCGCTCAACCTCTCCGATGCCCAGTCGCACCCGGCCTTCACCTATCTGCCGGA
GACGGGCGAAGAGATCTATCATTCCTTCCTGGGCGTTCCGATTCTACGCACCGGCCGTGCGCTCGGGGTGCTGGTCGTGC
AGAACAAGGCGCAGCGCAACTATCGCGAGGACGAGGTCGAGGCGCTGGAAACGACCGCCATGGTACTGGCCGAAATGGTC
GCGGCCGGCGAGCTCAAGAAGATCACCAAACCCGGGCTGGAACTCGATCTTTCGCGTCCGGTGACGATCGATGGCAATAG
CTACGGCGAAGGCATCGGGCTCGGTTACGTCGTTCTGCATGAGCCGCGCATCGTCGTTACCAATCTGCTCAACGAAGACA
CGGAGCAGGAACTGCAGCGTCTTGCAGAGGCGCTCGGGTCACTGCGCATCTCGATCGACGACATGCTGTCGCGCCGCGAC
GTATCGATGGAGGGCGAGCACCGCGCGGTGCTCGAGACCTATCGCATGTTCGCCCATGACCGCGGTTGGGTGAGAAAACT
CGAGGAGGCGATTCGCAACGGTCTGACGGCGGAAGCGGCGGTCGAGCGGGTGCAGAGCGAAACCAAGGCCCGCATGATAC
GCCTGACCGATCCCTATCTCAGGGAACGGATGCACGATTTCGACGATCTTGCGAACCGGCTGTTGCGGCAGCTTTCCGGT
TACGGCGCCAAGCGTTCGGCTGCCGACTTCCCAAATGACGCAATTGTCGTAGCCCGTGCCATGGGCGCCGCGGAGCTACT
CGACTATCCGCGAGAAAACGTGCGCGGTCTCGTGCTGGAAGAGGGCGCCGTCACCAGTCATGTGGTGATCGTCGCCCGGG
CCATGGGCATTCCGGTCGTGGGACAGGCGGCAGGCGCAGTGGCGCTTGCGGAAAACCGAGATGCGATCATCGTCGACGGC
GACGATGCGAAGGTGCATCTGCGGCCAATGGCGGACCTCCAGCGCGCATACGAGGAAAAGGTACGTTTCCGCGCGCGCAG
ACAGGCGCAGTTCCGTGCACTCAGGGACGTCGAACCGCTGACGAAGGACGGCAAGCGCATCACCTTGCAGATGAATGCCG
GCCTGCTGGTCGACTTGCCCCATCTGAACGAAGCCGGTGCCGAAGGTATCGGGCTGTTCCGTACCGAGCTGCAATTCATG
ATCGCTTCGACCATGCCGAAAGCCGAGGAGCAGGAGGTCTTTTATCGCAATGTCTTGAAGCAGACAGGGACCAAGCCGGT
GACCTTCCGGACCCTGGATATCGGCGGCGACAAGGTCGTCCCCTATTTCCGGGCAGCGGATGAGGAGAATCCGGCACTCG
GCTGGCGCGCCATCAGGCTGTCGCTCGACCGGCCGGGCCTCCTGCGCACCCAGTTCAGGGCTATGCTCAAGGCGACGGCC
GGCGCCGAGCTCAAGATGATGCTGCCGATGGTAACGGAAGTGACCGAGCTCAAGGCCGCGCGCGGACTTCTGCAAAAGGA
GATCGAGCGTCAGTCGAAGCTCGGCGAACAATTGCCGCGCAAGCTGCAGTTCGGGGCGATGCTGGAAGTGCCCGCCCTGC
TTTGGCAGCTCGACGAATTAATGGCCGAGGTCGATTTCGTTTCGGTCGGCTCAAACGATCTCTTCCAGTTCGCCATGGCT
GTCGACCGCGGCAATGCCCGTGTTTCCGATCGCTTCGATGTTCTCGGCCGGCCGTTCCTGCGGATACTCCGGGAGATCGT
GCGTGCTGGCGAACGCAACTGCACACCCGTGACCCTTTGCGGGGAAATGGCGAGCAAACCGCTTTCCGCCATGGCGCTGC
TCGGACTTGGTTTCCGGTCGGTTTCGATGTCGCCGACGGCTGTCGGGCCGGTGAAGGCGATGCTTCTGGCGCTCGACGCC
GGCAGGCTCAACGAACGCCTCGAGGCTGCGCTCGACGACGTCAAATCCGACGCGTCGATCCGCCAGCTCCTGGTCGATTT
CGCTGCCGCGAACGGCATACCGGTTTAG

Upstream 100 bases:

>100_bases
GCATGACGTGTCCTTGAATCGACGCCGCTTCAGGGAGCATGCAGAGGCCGCGGCGTCCACACGGGCCCGACGTTTATTCT
TATTCAGGAGACCCCACGCG

Downstream 100 bases:

>100_bases
ATTGTAAAAAGAGCTCTCGTCTTGTCCCGGACCCGATCCGTGCGAGCCGTGTGCATTTGGAGTGAGATTTGGCAAAGCTT
CCCGTTGAAAAGATGCGTGA

Product: phosphoenolpyruvate-protein phosphotransferase PtsP

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 755; Mature: 755

Protein sequence:

>755_residues
MRDLSAGPRVLLKRLRELMAEPLEPQERLDRIVRQIAQNMVAEVCSVYVLRSDGVLELYATEGLNKTAVHLAQLKMGQGL
VGTIAASARPLNLSDAQSHPAFTYLPETGEEIYHSFLGVPILRTGRALGVLVVQNKAQRNYREDEVEALETTAMVLAEMV
AAGELKKITKPGLELDLSRPVTIDGNSYGEGIGLGYVVLHEPRIVVTNLLNEDTEQELQRLAEALGSLRISIDDMLSRRD
VSMEGEHRAVLETYRMFAHDRGWVRKLEEAIRNGLTAEAAVERVQSETKARMIRLTDPYLRERMHDFDDLANRLLRQLSG
YGAKRSAADFPNDAIVVARAMGAAELLDYPRENVRGLVLEEGAVTSHVVIVARAMGIPVVGQAAGAVALAENRDAIIVDG
DDAKVHLRPMADLQRAYEEKVRFRARRQAQFRALRDVEPLTKDGKRITLQMNAGLLVDLPHLNEAGAEGIGLFRTELQFM
IASTMPKAEEQEVFYRNVLKQTGTKPVTFRTLDIGGDKVVPYFRAADEENPALGWRAIRLSLDRPGLLRTQFRAMLKATA
GAELKMMLPMVTEVTELKAARGLLQKEIERQSKLGEQLPRKLQFGAMLEVPALLWQLDELMAEVDFVSVGSNDLFQFAMA
VDRGNARVSDRFDVLGRPFLRILREIVRAGERNCTPVTLCGEMASKPLSAMALLGLGFRSVSMSPTAVGPVKAMLLALDA
GRLNERLEAALDDVKSDASIRQLLVDFAAANGIPV

Sequences:

>Translated_755_residues
MRDLSAGPRVLLKRLRELMAEPLEPQERLDRIVRQIAQNMVAEVCSVYVLRSDGVLELYATEGLNKTAVHLAQLKMGQGL
VGTIAASARPLNLSDAQSHPAFTYLPETGEEIYHSFLGVPILRTGRALGVLVVQNKAQRNYREDEVEALETTAMVLAEMV
AAGELKKITKPGLELDLSRPVTIDGNSYGEGIGLGYVVLHEPRIVVTNLLNEDTEQELQRLAEALGSLRISIDDMLSRRD
VSMEGEHRAVLETYRMFAHDRGWVRKLEEAIRNGLTAEAAVERVQSETKARMIRLTDPYLRERMHDFDDLANRLLRQLSG
YGAKRSAADFPNDAIVVARAMGAAELLDYPRENVRGLVLEEGAVTSHVVIVARAMGIPVVGQAAGAVALAENRDAIIVDG
DDAKVHLRPMADLQRAYEEKVRFRARRQAQFRALRDVEPLTKDGKRITLQMNAGLLVDLPHLNEAGAEGIGLFRTELQFM
IASTMPKAEEQEVFYRNVLKQTGTKPVTFRTLDIGGDKVVPYFRAADEENPALGWRAIRLSLDRPGLLRTQFRAMLKATA
GAELKMMLPMVTEVTELKAARGLLQKEIERQSKLGEQLPRKLQFGAMLEVPALLWQLDELMAEVDFVSVGSNDLFQFAMA
VDRGNARVSDRFDVLGRPFLRILREIVRAGERNCTPVTLCGEMASKPLSAMALLGLGFRSVSMSPTAVGPVKAMLLALDA
GRLNERLEAALDDVKSDASIRQLLVDFAAANGIPV
>Mature_755_residues
MRDLSAGPRVLLKRLRELMAEPLEPQERLDRIVRQIAQNMVAEVCSVYVLRSDGVLELYATEGLNKTAVHLAQLKMGQGL
VGTIAASARPLNLSDAQSHPAFTYLPETGEEIYHSFLGVPILRTGRALGVLVVQNKAQRNYREDEVEALETTAMVLAEMV
AAGELKKITKPGLELDLSRPVTIDGNSYGEGIGLGYVVLHEPRIVVTNLLNEDTEQELQRLAEALGSLRISIDDMLSRRD
VSMEGEHRAVLETYRMFAHDRGWVRKLEEAIRNGLTAEAAVERVQSETKARMIRLTDPYLRERMHDFDDLANRLLRQLSG
YGAKRSAADFPNDAIVVARAMGAAELLDYPRENVRGLVLEEGAVTSHVVIVARAMGIPVVGQAAGAVALAENRDAIIVDG
DDAKVHLRPMADLQRAYEEKVRFRARRQAQFRALRDVEPLTKDGKRITLQMNAGLLVDLPHLNEAGAEGIGLFRTELQFM
IASTMPKAEEQEVFYRNVLKQTGTKPVTFRTLDIGGDKVVPYFRAADEENPALGWRAIRLSLDRPGLLRTQFRAMLKATA
GAELKMMLPMVTEVTELKAARGLLQKEIERQSKLGEQLPRKLQFGAMLEVPALLWQLDELMAEVDFVSVGSNDLFQFAMA
VDRGNARVSDRFDVLGRPFLRILREIVRAGERNCTPVTLCGEMASKPLSAMALLGLGFRSVSMSPTAVGPVKAMLLALDA
GRLNERLEAALDDVKSDASIRQLLVDFAAANGIPV

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=721, Percent_Identity=35.506241331484, Blast_Score=367, Evalue=1e-102,
Organism=Escherichia coli, GI1788756, Length=532, Percent_Identity=31.203007518797, Blast_Score=270, Evalue=3e-73,
Organism=Escherichia coli, GI1788726, Length=587, Percent_Identity=32.1976149914821, Blast_Score=264, Evalue=2e-71,
Organism=Escherichia coli, GI48994992, Length=509, Percent_Identity=32.4165029469548, Blast_Score=251, Evalue=2e-67,
Organism=Escherichia coli, GI1787994, Length=411, Percent_Identity=25.3041362530414, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 83477; Mature: 83477

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00742 PEP_ENZYMES_2 ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDLSAGPRVLLKRLRELMAEPLEPQERLDRIVRQIAQNMVAEVCSVYVLRSDGVLELYA
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEE
TEGLNKTAVHLAQLKMGQGLVGTIAASARPLNLSDAQSHPAFTYLPETGEEIYHSFLGVP
CCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCC
ILRTGRALGVLVVQNKAQRNYREDEVEALETTAMVLAEMVAAGELKKITKPGLELDLSRP
HHHCCCEEEEEEEECCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCC
VTIDGNSYGEGIGLGYVVLHEPRIVVTNLLNEDTEQELQRLAEALGSLRISIDDMLSRRD
EEECCCCCCCCCCEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHEEECHHHHHHHCC
VSMEGEHRAVLETYRMFAHDRGWVRKLEEAIRNGLTAEAAVERVQSETKARMIRLTDPYL
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEECCHHH
RERMHDFDDLANRLLRQLSGYGAKRSAADFPNDAIVVARAMGAAELLDYPRENVRGLVLE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHCHHHHHHCCHHHCCEEEEE
EGAVTSHVVIVARAMGIPVVGQAAGAVALAENRDAIIVDGDDAKVHLRPMADLQRAYEEK
CCCCHHHHHHHHHHHCCCEECCCCCCEEEECCCCEEEEECCCCEEEECCHHHHHHHHHHH
VRFRARRQAQFRALRDVEPLTKDGKRITLQMNAGLLVDLPHLNEAGAEGIGLFRTELQFM
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHH
IASTMPKAEEQEVFYRNVLKQTGTKPVTFRTLDIGGDKVVPYFRAADEENPALGWRAIRL
HHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHCCCCCCCCCCEEEEEE
SLDRPGLLRTQFRAMLKATAGAELKMMLPMVTEVTELKAARGLLQKEIERQSKLGEQLPR
ECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLQFGAMLEVPALLWQLDELMAEVDFVSVGSNDLFQFAMAVDRGNARVSDRFDVLGRPFL
HHHCCCHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHCCCCCCCHHHHHCCCHHH
RILREIVRAGERNCTPVTLCGEMASKPLSAMALLGLGFRSVSMSPTAVGPVKAMLLALDA
HHHHHHHHCCCCCCCEEEECHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCC
GRLNERLEAALDDVKSDASIRQLLVDFAAANGIPV
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRDLSAGPRVLLKRLRELMAEPLEPQERLDRIVRQIAQNMVAEVCSVYVLRSDGVLELYA
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEE
TEGLNKTAVHLAQLKMGQGLVGTIAASARPLNLSDAQSHPAFTYLPETGEEIYHSFLGVP
CCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCC
ILRTGRALGVLVVQNKAQRNYREDEVEALETTAMVLAEMVAAGELKKITKPGLELDLSRP
HHHCCCEEEEEEEECCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCC
VTIDGNSYGEGIGLGYVVLHEPRIVVTNLLNEDTEQELQRLAEALGSLRISIDDMLSRRD
EEECCCCCCCCCCEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHEEECHHHHHHHCC
VSMEGEHRAVLETYRMFAHDRGWVRKLEEAIRNGLTAEAAVERVQSETKARMIRLTDPYL
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEECCHHH
RERMHDFDDLANRLLRQLSGYGAKRSAADFPNDAIVVARAMGAAELLDYPRENVRGLVLE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHCHHHHHHCCHHHCCEEEEE
EGAVTSHVVIVARAMGIPVVGQAAGAVALAENRDAIIVDGDDAKVHLRPMADLQRAYEEK
CCCCHHHHHHHHHHHCCCEECCCCCCEEEECCCCEEEEECCCCEEEECCHHHHHHHHHHH
VRFRARRQAQFRALRDVEPLTKDGKRITLQMNAGLLVDLPHLNEAGAEGIGLFRTELQFM
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHH
IASTMPKAEEQEVFYRNVLKQTGTKPVTFRTLDIGGDKVVPYFRAADEENPALGWRAIRL
HHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHCCCCCCCCCCEEEEEE
SLDRPGLLRTQFRAMLKATAGAELKMMLPMVTEVTELKAARGLLQKEIERQSKLGEQLPR
ECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLQFGAMLEVPALLWQLDELMAEVDFVSVGSNDLFQFAMAVDRGNARVSDRFDVLGRPFL
HHHCCCHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHCCCCCCCHHHHHCCCHHH
RILREIVRAGERNCTPVTLCGEMASKPLSAMALLGLGFRSVSMSPTAVGPVKAMLLALDA
HHHHHHHHCCCCCCCEEEECHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCC
GRLNERLEAALDDVKSDASIRQLLVDFAAANGIPV
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]