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The map label for this gene is prfA [H]

Identifier: 150397700

GI number: 150397700

Start: 2597447

End: 2598529

Strand: Reverse

Name: prfA [H]

Synonym: Smed_2502

Alternate gene names: 150397700

Gene position: 2598529-2597447 (Counterclockwise)

Preceding gene: 150397701

Following gene: 150397699

Centisome position: 68.71

GC content: 62.42

Gene sequence:

>1083_bases
TTGGCAAAGCTTCCCGTTGAAAAGATGCGTGAGCTGGAGCGGCGGTTCGGCGAAATCGAGGCGCGCATGTCGGCCGGACC
CTCGGCTGACGTCTACGTAAAGCTTGCCTCGGAATATTCCGAGTTGCAGCCGGTCGTATCGAAGATCCGCGCCTATGAAA
AAGCGACTGCCGAGCTTGCCGACATCGGCGCAATGCTCGCAGACCGGGCGACGGACAAGGACATGCGCGACCTCGCGGAG
CTGGAAAAGCCAGAGATCGAGGAGCGGGTCGAAGCGCTCGAGCAGGAAATCCAGATACTCCTCCTGCCGAAGGATGCGGC
TGACGAAAAGAGCGCGATTCTCGAAATCCGCGCCGGGACGGGTGGCTCGGAAGCCGCCCTCTTTGCTGGCGATCTTTTCC
GCATGTATGAGCGATATGCCGCAGGCAAGGGCTGGCGCGTCGAAGTGCTGTCGGCGAGCGAGGGCGAGGCCGGCGGCTAC
AAAGAAATCATCGCCACGGTATCCGGCCGCGGTGTGTTCTCACGGCTGAAATTCGAGTCCGGCGTCCACCGCGTGCAGCG
TGTACCCGAGACGGAAGCCGGTGGCCGTATCCATACCTCGGCGGCGACCGTAGCAGTGTTACCGGAGGCCGAAGATATCG
ACATCGACATCCGGTCGGAGGACATCCGCGTCGACACGATGCGCTCCTCGGGCGCAGGCGGCCAGCACGTCAACACGACG
GATTCGGCGGTGCGAATCACCCATCTTCCGACCGGTCTCGTCGTCACCAGTTCGGAAAAGTCGCAGCATCAGAACCGTGC
CAAGGCCATGCAGGTGCTGCGCTCCCGGCTCTACGACATGGAGCGGCAGCGCGCCGACAGCGAACGTTCGGCCGACCGCA
GGAACCAGGTAGGCTCCGGCGATCGTTCCGAGCGCATACGCACCTATAATTTCCCGCAGGGGCGCCTGACCGACCACCGC
ATCAATCTCACGATCTATAAGCTCGACCGGGTGATGGAGGGTGAGATCGACGAGATCGTGGATGCGCTTCTTGCCGACTA
TCAGGCGAGCCAGCTGGCGCTGCTCGGCGAAAAGCAGAACTGA

Upstream 100 bases:

>100_bases
ATTTCGCTGCCGCGAACGGCATACCGGTTTAGATTGTAAAAAGAGCTCTCGTCTTGTCCCGGACCCGATCCGTGCGAGCC
GTGTGCATTTGGAGTGAGAT

Downstream 100 bases:

>100_bases
GCGTCGATGCCCGAAACCCTCGACAACCTTCTTGCCGAGACCCGCGACCGGTTGAAGGCCGCCGGCATCGAATCGGCGGC
ACTCGATGCGCGGCACCTGG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MAKLPVEKMRELERRFGEIEARMSAGPSADVYVKLASEYSELQPVVSKIRAYEKATAELADIGAMLADRATDKDMRDLAE
LEKPEIEERVEALEQEIQILLLPKDAADEKSAILEIRAGTGGSEAALFAGDLFRMYERYAAGKGWRVEVLSASEGEAGGY
KEIIATVSGRGVFSRLKFESGVHRVQRVPETEAGGRIHTSAATVAVLPEAEDIDIDIRSEDIRVDTMRSSGAGGQHVNTT
DSAVRITHLPTGLVVTSSEKSQHQNRAKAMQVLRSRLYDMERQRADSERSADRRNQVGSGDRSERIRTYNFPQGRLTDHR
INLTIYKLDRVMEGEIDEIVDALLADYQASQLALLGEKQN

Sequences:

>Translated_360_residues
MAKLPVEKMRELERRFGEIEARMSAGPSADVYVKLASEYSELQPVVSKIRAYEKATAELADIGAMLADRATDKDMRDLAE
LEKPEIEERVEALEQEIQILLLPKDAADEKSAILEIRAGTGGSEAALFAGDLFRMYERYAAGKGWRVEVLSASEGEAGGY
KEIIATVSGRGVFSRLKFESGVHRVQRVPETEAGGRIHTSAATVAVLPEAEDIDIDIRSEDIRVDTMRSSGAGGQHVNTT
DSAVRITHLPTGLVVTSSEKSQHQNRAKAMQVLRSRLYDMERQRADSERSADRRNQVGSGDRSERIRTYNFPQGRLTDHR
INLTIYKLDRVMEGEIDEIVDALLADYQASQLALLGEKQN
>Mature_359_residues
AKLPVEKMRELERRFGEIEARMSAGPSADVYVKLASEYSELQPVVSKIRAYEKATAELADIGAMLADRATDKDMRDLAEL
EKPEIEERVEALEQEIQILLLPKDAADEKSAILEIRAGTGGSEAALFAGDLFRMYERYAAGKGWRVEVLSASEGEAGGYK
EIIATVSGRGVFSRLKFESGVHRVQRVPETEAGGRIHTSAATVAVLPEAEDIDIDIRSEDIRVDTMRSSGAGGQHVNTTD
SAVRITHLPTGLVVTSSEKSQHQNRAKAMQVLRSRLYDMERQRADSERSADRRNQVGSGDRSERIRTYNFPQGRLTDHRI
NLTIYKLDRVMEGEIDEIVDALLADYQASQLALLGEKQN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=310, Percent_Identity=46.1290322580645, Blast_Score=281, Evalue=8e-76,
Organism=Homo sapiens, GI34577120, Length=354, Percent_Identity=37.8531073446328, Blast_Score=234, Evalue=8e-62,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=46.3768115942029, Blast_Score=194, Evalue=8e-50,
Organism=Escherichia coli, GI1787462, Length=359, Percent_Identity=55.4317548746518, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI2367172, Length=316, Percent_Identity=37.3417721518987, Blast_Score=198, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI17542784, Length=271, Percent_Identity=38.7453874538745, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=354, Percent_Identity=39.8305084745763, Blast_Score=246, Evalue=5e-66,
Organism=Drosophila melanogaster, GI19921226, Length=322, Percent_Identity=42.2360248447205, Blast_Score=227, Evalue=7e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39951; Mature: 39820

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLPVEKMRELERRFGEIEARMSAGPSADVYVKLASEYSELQPVVSKIRAYEKATAELA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
DIGAMLADRATDKDMRDLAELEKPEIEERVEALEQEIQILLLPKDAADEKSAILEIRAGT
HHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCEEEEEECCC
GGSEAALFAGDLFRMYERYAAGKGWRVEVLSASEGEAGGYKEIIATVSGRGVFSRLKFES
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHH
GVHRVQRVPETEAGGRIHTSAATVAVLPEAEDIDIDIRSEDIRVDTMRSSGAGGQHVNTT
HHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCEEEEECCCEEEEEECCCCCCCCCCCCC
DSAVRITHLPTGLVVTSSEKSQHQNRAKAMQVLRSRLYDMERQRADSERSADRRNQVGSG
CCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
DRSERIRTYNFPQGRLTDHRINLTIYKLDRVMEGEIDEIVDALLADYQASQLALLGEKQN
CCCCCCEEECCCCCCCCCEEEEEEEEEECHHHCCCHHHHHHHHHHHCCHHHEEECCCCCC
>Mature Secondary Structure 
AKLPVEKMRELERRFGEIEARMSAGPSADVYVKLASEYSELQPVVSKIRAYEKATAELA
CCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
DIGAMLADRATDKDMRDLAELEKPEIEERVEALEQEIQILLLPKDAADEKSAILEIRAGT
HHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCEEEEEECCC
GGSEAALFAGDLFRMYERYAAGKGWRVEVLSASEGEAGGYKEIIATVSGRGVFSRLKFES
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHH
GVHRVQRVPETEAGGRIHTSAATVAVLPEAEDIDIDIRSEDIRVDTMRSSGAGGQHVNTT
HHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCEEEEECCCEEEEEECCCCCCCCCCCCC
DSAVRITHLPTGLVVTSSEKSQHQNRAKAMQVLRSRLYDMERQRADSERSADRRNQVGSG
CCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
DRSERIRTYNFPQGRLTDHRINLTIYKLDRVMEGEIDEIVDALLADYQASQLALLGEKQN
CCCCCCEEECCCCCCCCCEEEEEEEEEECHHHCCCHHHHHHHHHHHCCHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]