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The map label for this gene is hemK [H]

Identifier: 150397699

GI number: 150397699

Start: 2596562

End: 2597440

Strand: Reverse

Name: hemK [H]

Synonym: Smed_2501

Alternate gene names: 150397699

Gene position: 2597440-2596562 (Counterclockwise)

Preceding gene: 150397700

Following gene: 150397698

Centisome position: 68.68

GC content: 62.23

Gene sequence:

>879_bases
ATGCCCGAAACCCTCGACAACCTTCTTGCCGAGACCCGCGACCGGTTGAAGGCCGCCGGCATCGAATCGGCGGCACTCGA
TGCGCGGCACCTGGTCTCCGGCTTGCTCGAACTCGCACTTGCCGCGCTCGTGACGCGCGGGAGGGAGCCCGTCAGCGACG
AGGATGCGGCGCGCATCCGTGCCGCGGTCGAGCGCCGGGCTGCGCACGAGCCAGTCTATAGGATCCTCGGTGAGCGGGAA
TTTTCCGGCCTGAAGCTCAAGCTCTCGAAGGAGACGCTGGAGCCGCGCCCGGACACCGAGACCATGGTCGAATGCCTAAT
TCCCCACGCCCGGCGGATCGCCTTGAAAAAAGGGAGTTGCCGCATCGTCGACCTTGGAACGGGCACGGGTGCGATTTGTC
TCGCGCTTCTCGATGCGGTACTTGACGCGCGCGGCCTCGGTACCGATATATCGGAGGACGCATTGGCGACGGCATGTGAA
AATGCCCGCAGGAATGGCTTGGCGGGGCGCTTCGAAACGCTTCGGAGCAATTGGCTCGAGGCGGTGAATGGCCGGTTCGA
CATCATCGTCTCAAATCCGCCTTATATCCGGTCTAATGTCATTCCAGACCTTGAGCCGGAAGTGAAATTCCACGATCCGG
CCGCCGCGCTTGATGGCGGAGAGGATGGGCTGAATGCCTATCGTGCCATCGCTTCCGACGCTGGTCGCCATCTTGAACCA
GACGGCGTGATAGGCTTGGAAATCGGTTTCGACCAAAAGCAAGCGGTAACGGCGCTCTTCGAGGCGCATGGCTTTCATAT
GCTTTATGCCGCGAAGGACCTCGGCGGCAACGACCGGGTCCTGGTGTTCGAGCATGATCCTGCAGCGCCGCGCGTCTGA

Upstream 100 bases:

>100_bases
GGTGATGGAGGGTGAGATCGACGAGATCGTGGATGCGCTTCTTGCCGACTATCAGGCGAGCCAGCTGGCGCTGCTCGGCG
AAAAGCAGAACTGAGCGTCG

Downstream 100 bases:

>100_bases
CAAACGCGCAAACGTCGCGGCAGGGCATCGAGCTGCTGCATGTTTTGTCCTTCGATCGAGTACGAGCGAAGGAAACATGT
AGTGAACGCAGCAGCAACCG

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.MloHemKP [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MPETLDNLLAETRDRLKAAGIESAALDARHLVSGLLELALAALVTRGREPVSDEDAARIRAAVERRAAHEPVYRILGERE
FSGLKLKLSKETLEPRPDTETMVECLIPHARRIALKKGSCRIVDLGTGTGAICLALLDAVLDARGLGTDISEDALATACE
NARRNGLAGRFETLRSNWLEAVNGRFDIIVSNPPYIRSNVIPDLEPEVKFHDPAAALDGGEDGLNAYRAIASDAGRHLEP
DGVIGLEIGFDQKQAVTALFEAHGFHMLYAAKDLGGNDRVLVFEHDPAAPRV

Sequences:

>Translated_292_residues
MPETLDNLLAETRDRLKAAGIESAALDARHLVSGLLELALAALVTRGREPVSDEDAARIRAAVERRAAHEPVYRILGERE
FSGLKLKLSKETLEPRPDTETMVECLIPHARRIALKKGSCRIVDLGTGTGAICLALLDAVLDARGLGTDISEDALATACE
NARRNGLAGRFETLRSNWLEAVNGRFDIIVSNPPYIRSNVIPDLEPEVKFHDPAAALDGGEDGLNAYRAIASDAGRHLEP
DGVIGLEIGFDQKQAVTALFEAHGFHMLYAAKDLGGNDRVLVFEHDPAAPRV
>Mature_291_residues
PETLDNLLAETRDRLKAAGIESAALDARHLVSGLLELALAALVTRGREPVSDEDAARIRAAVERRAAHEPVYRILGEREF
SGLKLKLSKETLEPRPDTETMVECLIPHARRIALKKGSCRIVDLGTGTGAICLALLDAVLDARGLGTDISEDALATACEN
ARRNGLAGRFETLRSNWLEAVNGRFDIIVSNPPYIRSNVIPDLEPEVKFHDPAAALDGGEDGLNAYRAIASDAGRHLEPD
GVIGLEIGFDQKQAVTALFEAHGFHMLYAAKDLGGNDRVLVFEHDPAAPRV

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=245, Percent_Identity=32.6530612244898, Blast_Score=112, Evalue=6e-25,
Organism=Escherichia coli, GI1787463, Length=276, Percent_Identity=34.7826086956522, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI87082085, Length=255, Percent_Identity=29.8039215686275, Blast_Score=86, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324266, Length=168, Percent_Identity=30.952380952381, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582226, Length=263, Percent_Identity=29.6577946768061, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 31579; Mature: 31448

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPETLDNLLAETRDRLKAAGIESAALDARHLVSGLLELALAALVTRGREPVSDEDAARIR
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
AAVERRAAHEPVYRILGEREFSGLKLKLSKETLEPRPDTETMVECLIPHARRIALKKGSC
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHEECCCE
RIVDLGTGTGAICLALLDAVLDARGLGTDISEDALATACENARRNGLAGRFETLRSNWLE
EEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AVNGRFDIIVSNPPYIRSNVIPDLEPEVKFHDPAAALDGGEDGLNAYRAIASDAGRHLEP
HHCCCEEEEECCCCCHHHCCCCCCCCCCEECCCHHHCCCCHHHHHHHHHHHHHCCCCCCC
DGVIGLEIGFDQKQAVTALFEAHGFHMLYAAKDLGGNDRVLVFEHDPAAPRV
CCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
PETLDNLLAETRDRLKAAGIESAALDARHLVSGLLELALAALVTRGREPVSDEDAARIR
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
AAVERRAAHEPVYRILGEREFSGLKLKLSKETLEPRPDTETMVECLIPHARRIALKKGSC
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHEECCCE
RIVDLGTGTGAICLALLDAVLDARGLGTDISEDALATACENARRNGLAGRFETLRSNWLE
EEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AVNGRFDIIVSNPPYIRSNVIPDLEPEVKFHDPAAALDGGEDGLNAYRAIASDAGRHLEP
HHCCCEEEEECCCCCHHHCCCCCCCCCCEECCCHHHCCCCHHHHHHHHHHHHHCCCCCCC
DGVIGLEIGFDQKQAVTALFEAHGFHMLYAAKDLGGNDRVLVFEHDPAAPRV
CCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]