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The map label for this gene is mdeA [H]

Identifier: 150397619

GI number: 150397619

Start: 2496897

End: 2498069

Strand: Direct

Name: mdeA [H]

Synonym: Smed_2419

Alternate gene names: 150397619

Gene position: 2496897-2498069 (Clockwise)

Preceding gene: 150397618

Following gene: 150397620

Centisome position: 66.02

GC content: 62.83

Gene sequence:

>1173_bases
ATGAGCAACGGTCTCGATCCGTTCGAGAGAGCCTCCCTGATTGTCGCGCATGACGAGAGCAACGCTTTCGATGCCGTCGT
TCCCCCGATCGTCCAGACCTCGCTCTTCACCTTCAAGGATTATGACGAGATGCTCTCCTCCTATCGAGGCGAGCGCGTGC
GGCCGATCTACACCCGCGGCCTCAATCCGACGGTCAGGCATTTCGAGGAGATGCTGGCAAAGCTCGAAGGCGCCGAGGAT
GCGATCGGCTTCGCCAGCGGCATGTCGGCGATCTCATCGAGCGTCCTCTCCTTCGTGGAGCCGGGCGACCGCATCGTCTC
CGTGAAGCATGTGTATCCCGATGCGTTCCGCCTCTTCGGCACCCATCTGAAGCGCATGCGCATCGAAGTCACCTATGTCG
ACGGACGAGACGAGGAGGCGGTGGCGAGAGCACTTCCCGGCGCCAGGCTCTTCTACATGGAAAGCCCGACCAGCTGGGTC
ATGGACACGCATGATGTCGCATCGCTTGCGGCTCTTGCACGAGAGCAAGGGATCGTCACGGTCATCGACAACAGCTGGGC
GAGCCCGGTATTTCAGCAGCCGATCTCGCTTGGCGTCGACCTCGTCATTCACTCGGCCTCGAAATATCTGAGCGGCCATA
GCGATGTCGTCGCCGGGGTGGTCGCCGGGTCGAGCGCGTTGATCGGCCGCATCCGTTCGGAAGCCTATCCCTATCTCGGT
GGCAAGCTTTCACCATTCGATGCCTGGCTCCTGATCCGCGGCTTGCGCACCCTGCCGATCCGGATGAAGGCACATGAGCG
CTCGGCGCTTGCAGTCGCCCGTCGCCTGGCGGAGCATCCGCTGGTCGAAACCGTTTGCCATCCCGGCCTCGGCAATCATC
TTCCGCCGGGTCTCTCCGGCACCTCCGGCCTCTTCTCCTTCATCTTCCGCGAAGGCGTCGACGTGCGGCGCTTCGCCGAT
CATCTGAAACTGTTCAAGCTCGGCGTCTCCTGGGGCGGCCACGAAAGCCTGATCGTGCCGGGCGAAATCGTCCTCCGACA
GAAAGCCGAGCCCAATTCCGCGGCAGCCTTCGGCATTTCACCGCGGTCGGTACGGCTCCATGTCGGCCTGGAGGGAACGG
AGGCCCTCTGGAGCGACCTCGAAGCGGCGATGACAGCCGCTTCATCAGGCTGA

Upstream 100 bases:

>100_bases
CGCTGTTCAACGCGATCGCGGCTAAGGACGCCGAGGCCGCGCGGGCGGAAACGCTGAAAATCCTCGCAGTCGTCGAGGAA
GACATCAAGGAAATGTCCAA

Downstream 100 bases:

>100_bases
CGAACACGCAACCAGCGAGAACAAAGGGAACAAAAATGAGGAAACTGATCACAGCCGCCCTGCTTGCCACCCTGATGGCC
GGCAGCGCCCTTGCAGATAC

Product: hypothetical protein

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSNGLDPFERASLIVAHDESNAFDAVVPPIVQTSLFTFKDYDEMLSSYRGERVRPIYTRGLNPTVRHFEEMLAKLEGAED
AIGFASGMSAISSSVLSFVEPGDRIVSVKHVYPDAFRLFGTHLKRMRIEVTYVDGRDEEAVARALPGARLFYMESPTSWV
MDTHDVASLAALAREQGIVTVIDNSWASPVFQQPISLGVDLVIHSASKYLSGHSDVVAGVVAGSSALIGRIRSEAYPYLG
GKLSPFDAWLLIRGLRTLPIRMKAHERSALAVARRLAEHPLVETVCHPGLGNHLPPGLSGTSGLFSFIFREGVDVRRFAD
HLKLFKLGVSWGGHESLIVPGEIVLRQKAEPNSAAAFGISPRSVRLHVGLEGTEALWSDLEAAMTAASSG

Sequences:

>Translated_390_residues
MSNGLDPFERASLIVAHDESNAFDAVVPPIVQTSLFTFKDYDEMLSSYRGERVRPIYTRGLNPTVRHFEEMLAKLEGAED
AIGFASGMSAISSSVLSFVEPGDRIVSVKHVYPDAFRLFGTHLKRMRIEVTYVDGRDEEAVARALPGARLFYMESPTSWV
MDTHDVASLAALAREQGIVTVIDNSWASPVFQQPISLGVDLVIHSASKYLSGHSDVVAGVVAGSSALIGRIRSEAYPYLG
GKLSPFDAWLLIRGLRTLPIRMKAHERSALAVARRLAEHPLVETVCHPGLGNHLPPGLSGTSGLFSFIFREGVDVRRFAD
HLKLFKLGVSWGGHESLIVPGEIVLRQKAEPNSAAAFGISPRSVRLHVGLEGTEALWSDLEAAMTAASSG
>Mature_389_residues
SNGLDPFERASLIVAHDESNAFDAVVPPIVQTSLFTFKDYDEMLSSYRGERVRPIYTRGLNPTVRHFEEMLAKLEGAEDA
IGFASGMSAISSSVLSFVEPGDRIVSVKHVYPDAFRLFGTHLKRMRIEVTYVDGRDEEAVARALPGARLFYMESPTSWVM
DTHDVASLAALAREQGIVTVIDNSWASPVFQQPISLGVDLVIHSASKYLSGHSDVVAGVVAGSSALIGRIRSEAYPYLGG
KLSPFDAWLLIRGLRTLPIRMKAHERSALAVARRLAEHPLVETVCHPGLGNHLPPGLSGTSGLFSFIFREGVDVRRFADH
LKLFKLGVSWGGHESLIVPGEIVLRQKAEPNSAAAFGISPRSVRLHVGLEGTEALWSDLEAAMTAASSG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=372, Percent_Identity=34.6774193548387, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI299473759, Length=372, Percent_Identity=31.989247311828, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI25453487, Length=370, Percent_Identity=30.8108108108108, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI1790375, Length=380, Percent_Identity=36.5789473684211, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1789383, Length=343, Percent_Identity=32.6530612244898, Blast_Score=182, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17560056, Length=359, Percent_Identity=35.0974930362117, Blast_Score=195, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17538055, Length=363, Percent_Identity=34.7107438016529, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI71980708, Length=381, Percent_Identity=32.5459317585302, Blast_Score=178, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6319307, Length=382, Percent_Identity=30.8900523560209, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6321170, Length=329, Percent_Identity=30.6990881458967, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6323333, Length=422, Percent_Identity=25.1184834123223, Blast_Score=122, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6321904, Length=389, Percent_Identity=25.1928020565553, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321254, Length=414, Percent_Identity=24.6376811594203, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19922554, Length=373, Percent_Identity=34.3163538873995, Blast_Score=188, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 42389; Mature: 42258

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNGLDPFERASLIVAHDESNAFDAVVPPIVQTSLFTFKDYDEMLSSYRGERVRPIYTRG
CCCCCCCHHHCEEEEEECCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
LNPTVRHFEEMLAKLEGAEDAIGFASGMSAISSSVLSFVEPGDRIVSVKHVYPDAFRLFG
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
THLKRMRIEVTYVDGRDEEAVARALPGARLFYMESPTSWVMDTHDVASLAALAREQGIVT
HHHHEEEEEEEEECCCCHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEE
VIDNSWASPVFQQPISLGVDLVIHSASKYLSGHSDVVAGVVAGSSALIGRIRSEAYPYLG
EECCCCCCHHHHHHHHHCHHHEEECHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCCCC
GKLSPFDAWLLIRGLRTLPIRMKAHERSALAVARRLAEHPLVETVCHPGLGNHLPPGLSG
CCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCC
TSGLFSFIFREGVDVRRFADHLKLFKLGVSWGGHESLIVPGEIVLRQKAEPNSAAAFGIS
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEECHHHEEECCCCCCCCEEECCC
PRSVRLHVGLEGTEALWSDLEAAMTAASSG
CCEEEEEECCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SNGLDPFERASLIVAHDESNAFDAVVPPIVQTSLFTFKDYDEMLSSYRGERVRPIYTRG
CCCCCCHHHCEEEEEECCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
LNPTVRHFEEMLAKLEGAEDAIGFASGMSAISSSVLSFVEPGDRIVSVKHVYPDAFRLFG
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
THLKRMRIEVTYVDGRDEEAVARALPGARLFYMESPTSWVMDTHDVASLAALAREQGIVT
HHHHEEEEEEEEECCCCHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEE
VIDNSWASPVFQQPISLGVDLVIHSASKYLSGHSDVVAGVVAGSSALIGRIRSEAYPYLG
EECCCCCCHHHHHHHHHCHHHEEECHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCCCC
GKLSPFDAWLLIRGLRTLPIRMKAHERSALAVARRLAEHPLVETVCHPGLGNHLPPGLSG
CCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCC
TSGLFSFIFREGVDVRRFADHLKLFKLGVSWGGHESLIVPGEIVLRQKAEPNSAAAFGIS
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEECHHHEEECCCCCCCCEEECCC
PRSVRLHVGLEGTEALWSDLEAAMTAASSG
CCEEEEEECCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]