Click here to switch to the map view.

The map label for this gene is lutR [H]

Identifier: 150397618

GI number: 150397618

Start: 2496184

End: 2496900

Strand: Direct

Name: lutR [H]

Synonym: Smed_2418

Alternate gene names: 150397618

Gene position: 2496184-2496900 (Clockwise)

Preceding gene: 150397617

Following gene: 150397619

Centisome position: 66.0

GC content: 62.76

Gene sequence:

>717_bases
ATGCGCCTGCAGGATAAAGCGATGCTCCTGCCCCTGCCGCCTGCGGATCGAGCGCAGCAGGTGATATCCGCACTTGCCGA
ATACATTCAAAGATCGGGGCTGAACCCGGGGGACCGGCTGCCGGCCGAGCGGGAACTAATGGCGGCGCTTGCCGTCGGCC
GCTCCACCATTCGCGAGGTGCTGAGCCATTTTCAGGCGCTCGGTGTCGTTGAGGCGCGCAAGGGCAGCGGTACCTATCTC
CTGCGCACCATTTCTCCCGGGACCATCCACATGCCGCTTGCACTCGATACCCGGCACCTGCGAGACGCCCTGCTGCAGAC
GCTTGAAGTGCGGCGCGGCATCGAGGTCGAGGCCGGCATGGTCGCCTCCAGACGCCGCACGGAAGCCGACCTCGTCAACA
TCGAGACGAAGCTCGACGAAATGGAACGGGTCCATCTCGCCAAGGGAACGTCGGGCCCGGAGGACCTCGCCTTTCATCTC
GCGATCTACGACGCGACCCATAATCCGCTGTTCAAGCAACTGCTCGAACAGATGCGCGAAGCTTTCGAGCGCTTCTGGGA
CGAGCCCTTCAACCGGTCGGACTTTGCCCGGCGCTCCTTTCCCTTTCACAGGACGCTGTTCAACGCGATCGCGGCTAAGG
ACGCCGAGGCCGCGCGGGCGGAAACGCTGAAAATCCTCGCAGTCGTCGAGGAAGACATCAAGGAAATGTCCAAATGA

Upstream 100 bases:

>100_bases
GCACCATTTCATCAATGAGGACCGAGGCTCTCTTGACCCTCTTTCCGATCCCCTGCATATTGCTCAGATGTCAGACCAAT
TCCGGTCTTGGGAGAATCGG

Downstream 100 bases:

>100_bases
GCAACGGTCTCGATCCGTTCGAGAGAGCCTCCCTGATTGTCGCGCATGACGAGAGCAACGCTTTCGATGCCGTCGTTCCC
CCGATCGTCCAGACCTCGCT

Product: GntR domain-containing protein

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRLQDKAMLLPLPPADRAQQVISALAEYIQRSGLNPGDRLPAERELMAALAVGRSTIREVLSHFQALGVVEARKGSGTYL
LRTISPGTIHMPLALDTRHLRDALLQTLEVRRGIEVEAGMVASRRRTEADLVNIETKLDEMERVHLAKGTSGPEDLAFHL
AIYDATHNPLFKQLLEQMREAFERFWDEPFNRSDFARRSFPFHRTLFNAIAAKDAEAARAETLKILAVVEEDIKEMSK

Sequences:

>Translated_238_residues
MRLQDKAMLLPLPPADRAQQVISALAEYIQRSGLNPGDRLPAERELMAALAVGRSTIREVLSHFQALGVVEARKGSGTYL
LRTISPGTIHMPLALDTRHLRDALLQTLEVRRGIEVEAGMVASRRRTEADLVNIETKLDEMERVHLAKGTSGPEDLAFHL
AIYDATHNPLFKQLLEQMREAFERFWDEPFNRSDFARRSFPFHRTLFNAIAAKDAEAARAETLKILAVVEEDIKEMSK
>Mature_238_residues
MRLQDKAMLLPLPPADRAQQVISALAEYIQRSGLNPGDRLPAERELMAALAVGRSTIREVLSHFQALGVVEARKGSGTYL
LRTISPGTIHMPLALDTRHLRDALLQTLEVRRGIEVEAGMVASRRRTEADLVNIETKLDEMERVHLAKGTSGPEDLAFHL
AIYDATHNPLFKQLLEQMREAFERFWDEPFNRSDFARRSFPFHRTLFNAIAAKDAEAARAETLKILAVVEEDIKEMSK

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=228, Percent_Identity=30.2631578947368, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI48994955, Length=237, Percent_Identity=27.8481012658228, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790032, Length=243, Percent_Identity=28.3950617283951, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786303, Length=222, Percent_Identity=28.3783783783784, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 26838; Mature: 26838

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLQDKAMLLPLPPADRAQQVISALAEYIQRSGLNPGDRLPAERELMAALAVGRSTIREV
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LSHFQALGVVEARKGSGTYLLRTISPGTIHMPLALDTRHLRDALLQTLEVRRGIEVEAGM
HHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHH
VASRRRTEADLVNIETKLDEMERVHLAKGTSGPEDLAFHLAIYDATHNPLFKQLLEQMRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEEEEECCCCHHHHHHHHHHHH
AFERFWDEPFNRSDFARRSFPFHRTLFNAIAAKDAEAARAETLKILAVVEEDIKEMSK
HHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLQDKAMLLPLPPADRAQQVISALAEYIQRSGLNPGDRLPAERELMAALAVGRSTIREV
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LSHFQALGVVEARKGSGTYLLRTISPGTIHMPLALDTRHLRDALLQTLEVRRGIEVEAGM
HHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHH
VASRRRTEADLVNIETKLDEMERVHLAKGTSGPEDLAFHLAIYDATHNPLFKQLLEQMRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEEEEECCCCHHHHHHHHHHHH
AFERFWDEPFNRSDFARRSFPFHRTLFNAIAAKDAEAARAETLKILAVVEEDIKEMSK
HHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]