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The map label for this gene is mcbR [H]

Identifier: 150397617

GI number: 150397617

Start: 2495315

End: 2496007

Strand: Direct

Name: mcbR [H]

Synonym: Smed_2417

Alternate gene names: 150397617

Gene position: 2495315-2496007 (Clockwise)

Preceding gene: 150397612

Following gene: 150397618

Centisome position: 65.98

GC content: 63.64

Gene sequence:

>693_bases
TTGAAGACCCTTGTCCTGCCCGCCCTTGATCGTCCGGAAGGCATCACGACTCACGACTATGCCTTCTCGCGCCTCAGGCA
GGCAATCATGGTCGGCGCCTTTCGTCCCGGCACCTCCGTCACCATACGCGGCCTCGCGGATGCGCTGGAGAGCAGCCCGA
CGCCCGTGCGCGAAGCCCTGCGGCAGCTGACCTCGATCGGCGCCCTGCAATTTCTCCAGAACAGGCGCATCGTCGTGCCG
CGGATATCGCCCGCGCGCTTCGAGGAGCTCATCTCTCTCCGCATCGTGCTTGAAAGCCACGCCGCCCGCAGGGCCGTTGC
TCATCTTTCCGACCGGCAGATCGACCGCATCGCGGTGCTGGACGATGGAATCGAGGCCGCGATTCTCTCCGGACAGAAAG
CGCAGGCGCTGATCGCCAATCAGGAATTTCACCGGCAGATCTATTCGGCCAATCCTGATCAGGTTGCTGTCCCGCTCATC
GAGAGCCTCTGGCTGCAGCTCGGCCCGATCCTCGGCATCGCCATGGAACACGTCAACGAATTCTACGTCGTCGATCGTCA
CCGCGAGGCCATCGATGCCCTGAGGCGGCGCGATGAGAACGCCGTAGCGGAAGCCATCGCCGCCGATATCCGCGAGGGAA
TAGGCGGCTTCGATCAGCAGGCGATCGGCAAGCTGCTCGCGCTTGCGGGATAA

Upstream 100 bases:

>100_bases
CACATTTATCAGAAATTGACATCTTTGATGATTTAGAGTCAAGCTGAAGCGAGCCGACAGGATCGGCTGGATCCAACGAC
CGGGTGCCGGAGTCGTCGCC

Downstream 100 bases:

>100_bases
ACGAGCTCTGGGTTTGCTCAGTCGGTTACCACCCTGGTTTGCCCCTCACCGCACGTCCCTTGCGGCCCCTTTCGCGGCAC
CATTTCATCAATGAGGACCG

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MKTLVLPALDRPEGITTHDYAFSRLRQAIMVGAFRPGTSVTIRGLADALESSPTPVREALRQLTSIGALQFLQNRRIVVP
RISPARFEELISLRIVLESHAARRAVAHLSDRQIDRIAVLDDGIEAAILSGQKAQALIANQEFHRQIYSANPDQVAVPLI
ESLWLQLGPILGIAMEHVNEFYVVDRHREAIDALRRRDENAVAEAIAADIREGIGGFDQQAIGKLLALAG

Sequences:

>Translated_230_residues
MKTLVLPALDRPEGITTHDYAFSRLRQAIMVGAFRPGTSVTIRGLADALESSPTPVREALRQLTSIGALQFLQNRRIVVP
RISPARFEELISLRIVLESHAARRAVAHLSDRQIDRIAVLDDGIEAAILSGQKAQALIANQEFHRQIYSANPDQVAVPLI
ESLWLQLGPILGIAMEHVNEFYVVDRHREAIDALRRRDENAVAEAIAADIREGIGGFDQQAIGKLLALAG
>Mature_230_residues
MKTLVLPALDRPEGITTHDYAFSRLRQAIMVGAFRPGTSVTIRGLADALESSPTPVREALRQLTSIGALQFLQNRRIVVP
RISPARFEELISLRIVLESHAARRAVAHLSDRQIDRIAVLDDGIEAAILSGQKAQALIANQEFHRQIYSANPDQVAVPLI
ESLWLQLGPILGIAMEHVNEFYVVDRHREAIDALRRRDENAVAEAIAADIREGIGGFDQQAIGKLLALAG

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=199, Percent_Identity=27.1356783919598, Blast_Score=81, Evalue=6e-17,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 25302; Mature: 25302

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLVLPALDRPEGITTHDYAFSRLRQAIMVGAFRPGTSVTIRGLADALESSPTPVREAL
CCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCCCHHHHHH
RQLTSIGALQFLQNRRIVVPRISPARFEELISLRIVLESHAARRAVAHLSDRQIDRIAVL
HHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEE
DDGIEAAILSGQKAQALIANQEFHRQIYSANPDQVAVPLIESLWLQLGPILGIAMEHVNE
CCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYVVDRHREAIDALRRRDENAVAEAIAADIREGIGGFDQQAIGKLLALAG
HHEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKTLVLPALDRPEGITTHDYAFSRLRQAIMVGAFRPGTSVTIRGLADALESSPTPVREAL
CCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCCCHHHHHH
RQLTSIGALQFLQNRRIVVPRISPARFEELISLRIVLESHAARRAVAHLSDRQIDRIAVL
HHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEE
DDGIEAAILSGQKAQALIANQEFHRQIYSANPDQVAVPLIESLWLQLGPILGIAMEHVNE
CCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYVVDRHREAIDALRRRDENAVAEAIAADIREGIGGFDQQAIGKLLALAG
HHEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]