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The map label for this gene is leuA [H]

Identifier: 150397487

GI number: 150397487

Start: 2363312

End: 2365042

Strand: Reverse

Name: leuA [H]

Synonym: Smed_2287

Alternate gene names: 150397487

Gene position: 2365042-2363312 (Counterclockwise)

Preceding gene: 150397488

Following gene: 150397486

Centisome position: 62.54

GC content: 59.56

Gene sequence:

>1731_bases
ATGATTTTGAAATCGCATTCCATCAAGACCGGCATGCCCGATGCGGCCGTGAAGTATCAGCCATACCCGCAGATTGCGCT
GCCGGATCGCACATGGCCGTCCAAAGTCATTGCCGAAGCACCGATCTGGTGTTCCGTGGACCTGCGCGACGGCAACCAGG
CACTCGTCGACCCGATGGGCCACGATCGCAAGGCACGCATGTTCCAACTCCTGCTGGACATGGGCTTCAAGGAAATCGAG
ATCGGCTTTCCTTCCGCGTCGCAGACCGATTTCGATTTTGCCCGCTGGTGCGTCGAGGAAAGCAACGTCTCCGAAGACGT
GTCTCTGCAGGTGCTGGTGCAGTGCCGGCCGGAGCTGATCTCGCGCACCTTCGAGGCACTCGAAGGTGCGCACCGGCCGA
TCGTGCACTTCTACAATTCGACGAGCGAACTGCAGCGCCGTGTGGTCTTCGGCAAGGACGTCTCCGGGATCAAGCAGATT
GCGACCGACGCCGCCAAGATGATTACCGACATGGCCGCGAAAGCGGGCGGCGGATATCGCTTCGAATATTCGCCGGAAAG
CTTCACCGGTACCGAGCTGGAAGTGGCGTTGGAGATCTGCAACGCGGTGATCGAAATCGTCCGGCCGACACAGGACAACA
AGCTCATCATCAATCTGCCGTCGACGGTCGAAATGGCGACGCCGAACATCTATGCCGATCAGATCGAATGGATGTGCCGC
AATCTCGACAATCGCGAGAACCTGCTCATCTCACTGCACCCGCACAACGATCGTGGCACGGGCATTGCTGCTACGGAACT
CGGTCTGATGGCCGGCGCCGACCGGGTCGAAGGGACGCTCTTCGGCAATGGAGAGCGCACCGGAAATGTCGATGTGGTGA
CGCTGGCGCTCAATATGTTCACGCAGGGCGTCGACCCCAAGCTCGACTGCTCGGACATAGAGCGGATCAAGGAGGTCTAC
GAATATTCCAACCAGATGGTCATTCCGGAACGGCACCCTTACGTAGGGGAGCTGGTGTACACCGCCTTCTCGGGCTCGCA
TCAGGACGCCATCAACAAGGGCATGAAGGCGATCAAACAGGCGAACAAGCCGGTCTGGGAGGTCCCCTACCTGCCGATCG
ACCCGCGTGACGTCGGGCGCAGCTACGAAGCGATCATCCGCATCAACTCGCAATCGGGCAAGGGCGGCATCGCCTATATC
CTGCAAGAGGATTACGGTATCAATCTGCCCCGCAATCTCCAGATCGAGTTTCGCGAGGAGATTCAGCGCATCACCGACGA
GGAGGGAAAGGAGCTGCCCTCGAAGCGCATCCATCAGCGCTTCATCGAAAGCTATGTCGACCAGCCGGGCGCCCGTATCA
AGTTCGTCGACCACCACACCTATCCGGTGGGCGAACACAAGGGCCTGCGCGTCGTTGCCGCGGAAATTACGGATCGCGGC
GAGACACGCCAGATCGAAGGCAAGGGCACGGGCCCGATCGACGGCTTCATCAATGCGTTGTCGATCTATCTCGGCATCGA
ACTTTCGGTGGCGGATTATTCCGAGCATTCGCTGCAGCATGGCTCGAACGCGGCTGCGATTGCCTATGTGGAGGTCGAGT
ATCCCGGCGGAAAACTCTTCGGCGTCGGCATCAACACCAATATCGTCGCCGCCTCCCTGGAGGCGATCGTCTCGGCTGCA
AACCGGGTGTTGGACGTCATGGGAAGTGACGCGCTTGCGCAATCACGCTAG

Upstream 100 bases:

>100_bases
CCAAATCGCGCGGCCCCGGCAGCATCGGTCATCGCAATGCCCCGAATGATCGGCTATTGCATGCGGCAGGCTACGCTCCA
GTGACGAAGAGAAGCTGAAC

Downstream 100 bases:

>100_bases
GCGGGAAATCCAATTTGTTATGTAGGGATATCCCGGAAAGGATATCCCTATGGCTCTCTACATCAAAGATCCTACCGTCG
GCCTAATGGTGGAGCAAAAT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MILKSHSIKTGMPDAAVKYQPYPQIALPDRTWPSKVIAEAPIWCSVDLRDGNQALVDPMGHDRKARMFQLLLDMGFKEIE
IGFPSASQTDFDFARWCVEESNVSEDVSLQVLVQCRPELISRTFEALEGAHRPIVHFYNSTSELQRRVVFGKDVSGIKQI
ATDAAKMITDMAAKAGGGYRFEYSPESFTGTELEVALEICNAVIEIVRPTQDNKLIINLPSTVEMATPNIYADQIEWMCR
NLDNRENLLISLHPHNDRGTGIAATELGLMAGADRVEGTLFGNGERTGNVDVVTLALNMFTQGVDPKLDCSDIERIKEVY
EYSNQMVIPERHPYVGELVYTAFSGSHQDAINKGMKAIKQANKPVWEVPYLPIDPRDVGRSYEAIIRINSQSGKGGIAYI
LQEDYGINLPRNLQIEFREEIQRITDEEGKELPSKRIHQRFIESYVDQPGARIKFVDHHTYPVGEHKGLRVVAAEITDRG
ETRQIEGKGTGPIDGFINALSIYLGIELSVADYSEHSLQHGSNAAAIAYVEVEYPGGKLFGVGINTNIVAASLEAIVSAA
NRVLDVMGSDALAQSR

Sequences:

>Translated_576_residues
MILKSHSIKTGMPDAAVKYQPYPQIALPDRTWPSKVIAEAPIWCSVDLRDGNQALVDPMGHDRKARMFQLLLDMGFKEIE
IGFPSASQTDFDFARWCVEESNVSEDVSLQVLVQCRPELISRTFEALEGAHRPIVHFYNSTSELQRRVVFGKDVSGIKQI
ATDAAKMITDMAAKAGGGYRFEYSPESFTGTELEVALEICNAVIEIVRPTQDNKLIINLPSTVEMATPNIYADQIEWMCR
NLDNRENLLISLHPHNDRGTGIAATELGLMAGADRVEGTLFGNGERTGNVDVVTLALNMFTQGVDPKLDCSDIERIKEVY
EYSNQMVIPERHPYVGELVYTAFSGSHQDAINKGMKAIKQANKPVWEVPYLPIDPRDVGRSYEAIIRINSQSGKGGIAYI
LQEDYGINLPRNLQIEFREEIQRITDEEGKELPSKRIHQRFIESYVDQPGARIKFVDHHTYPVGEHKGLRVVAAEITDRG
ETRQIEGKGTGPIDGFINALSIYLGIELSVADYSEHSLQHGSNAAAIAYVEVEYPGGKLFGVGINTNIVAASLEAIVSAA
NRVLDVMGSDALAQSR
>Mature_576_residues
MILKSHSIKTGMPDAAVKYQPYPQIALPDRTWPSKVIAEAPIWCSVDLRDGNQALVDPMGHDRKARMFQLLLDMGFKEIE
IGFPSASQTDFDFARWCVEESNVSEDVSLQVLVQCRPELISRTFEALEGAHRPIVHFYNSTSELQRRVVFGKDVSGIKQI
ATDAAKMITDMAAKAGGGYRFEYSPESFTGTELEVALEICNAVIEIVRPTQDNKLIINLPSTVEMATPNIYADQIEWMCR
NLDNRENLLISLHPHNDRGTGIAATELGLMAGADRVEGTLFGNGERTGNVDVVTLALNMFTQGVDPKLDCSDIERIKEVY
EYSNQMVIPERHPYVGELVYTAFSGSHQDAINKGMKAIKQANKPVWEVPYLPIDPRDVGRSYEAIIRINSQSGKGGIAYI
LQEDYGINLPRNLQIEFREEIQRITDEEGKELPSKRIHQRFIESYVDQPGARIKFVDHHTYPVGEHKGLRVVAAEITDRG
ETRQIEGKGTGPIDGFINALSIYLGIELSVADYSEHSLQHGSNAAAIAYVEVEYPGGKLFGVGINTNIVAASLEAIVSAA
NRVLDVMGSDALAQSR

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=523, Percent_Identity=24.0917782026769, Blast_Score=112, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6324225, Length=571, Percent_Identity=45.8844133099825, Blast_Score=516, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6324682, Length=574, Percent_Identity=45.4703832752613, Blast_Score=511, Evalue=1e-145,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 63870; Mature: 63870

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILKSHSIKTGMPDAAVKYQPYPQIALPDRTWPSKVIAEAPIWCSVDLRDGNQALVDPMG
CCCCCCCCCCCCCCCCEEECCCCCEECCCCCCCHHHHHCCCEEEEEEECCCCEEECCCCC
HDRKARMFQLLLDMGFKEIEIGFPSASQTDFDFARWCVEESNVSEDVSLQVLVQCRPELI
CCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCHHHH
SRTFEALEGAHRPIVHFYNSTSELQRRVVFGKDVSGIKQIATDAAKMITDMAAKAGGGYR
HHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
FEYSPESFTGTELEVALEICNAVIEIVRPTQDNKLIINLPSTVEMATPNIYADQIEWMCR
EEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCHHHHHHHHHH
NLDNRENLLISLHPHNDRGTGIAATELGLMAGADRVEGTLFGNGERTGNVDVVTLALNMF
CCCCCCCEEEEEECCCCCCCCEEHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEEHHH
TQGVDPKLDCSDIERIKEVYEYSNQMVIPERHPYVGELVYTAFSGSHQDAINKGMKAIKQ
HCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCHHHHHEECCCCCCHHHHHHHHHHHHH
ANKPVWEVPYLPIDPRDVGRSYEAIIRINSQSGKGGIAYILQEDYGINLPRNLQIEFREE
CCCCEEECCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCEEHHHHH
IQRITDEEGKELPSKRIHQRFIESYVDQPGARIKFVDHHTYPVGEHKGLRVVAAEITDRG
HHHHHCHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEEEEECCCC
ETRQIEGKGTGPIDGFINALSIYLGIELSVADYSEHSLQHGSNAAAIAYVEVEYPGGKLF
CCEEECCCCCCCHHHHHHHHHHHEEEEEEECCCCHHHHHCCCCCEEEEEEEEECCCCEEE
GVGINTNIVAASLEAIVSAANRVLDVMGSDALAQSR
EECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure
MILKSHSIKTGMPDAAVKYQPYPQIALPDRTWPSKVIAEAPIWCSVDLRDGNQALVDPMG
CCCCCCCCCCCCCCCCEEECCCCCEECCCCCCCHHHHHCCCEEEEEEECCCCEEECCCCC
HDRKARMFQLLLDMGFKEIEIGFPSASQTDFDFARWCVEESNVSEDVSLQVLVQCRPELI
CCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCHHHH
SRTFEALEGAHRPIVHFYNSTSELQRRVVFGKDVSGIKQIATDAAKMITDMAAKAGGGYR
HHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
FEYSPESFTGTELEVALEICNAVIEIVRPTQDNKLIINLPSTVEMATPNIYADQIEWMCR
EEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCHHHHHHHHHH
NLDNRENLLISLHPHNDRGTGIAATELGLMAGADRVEGTLFGNGERTGNVDVVTLALNMF
CCCCCCCEEEEEECCCCCCCCEEHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEEHHH
TQGVDPKLDCSDIERIKEVYEYSNQMVIPERHPYVGELVYTAFSGSHQDAINKGMKAIKQ
HCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCHHHHHEECCCCCCHHHHHHHHHHHHH
ANKPVWEVPYLPIDPRDVGRSYEAIIRINSQSGKGGIAYILQEDYGINLPRNLQIEFREE
CCCCEEECCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCEEHHHHH
IQRITDEEGKELPSKRIHQRFIESYVDQPGARIKFVDHHTYPVGEHKGLRVVAAEITDRG
HHHHHCHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEEEEECCCC
ETRQIEGKGTGPIDGFINALSIYLGIELSVADYSEHSLQHGSNAAAIAYVEVEYPGGKLF
CCEEECCCCCCCHHHHHHHHHHHEEEEEEECCCCHHHHHCCCCCEEEEEEEEECCCCEEE
GVGINTNIVAASLEAIVSAANRVLDVMGSDALAQSR
EECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12070767; 11481430 [H]