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The map label for this gene is ilvB [H]

Identifier: 150397322

GI number: 150397322

Start: 2184895

End: 2186541

Strand: Direct

Name: ilvB [H]

Synonym: Smed_2121

Alternate gene names: 150397322

Gene position: 2184895-2186541 (Clockwise)

Preceding gene: 150397321

Following gene: 150397323

Centisome position: 57.77

GC content: 65.63

Gene sequence:

>1647_bases
ATGAAAACGGGCGGACAACTGGTGGTGGAGGCGCTTGTCGCCAATGGGGTGAAGCGCATCTCCTGCGTTCCGGGCGAAAG
CTATCTGGCCGTGCTCGATGCGCTCTACGACTCGGACGTCGAAGTCGTCGTCTGCCGGCAGGAGGGCGGCGCGGCCATGA
TGGCGGATGCTTGGGGACGGCTGACGGGCGAACCGGGCATCTGCATGGTCACCCGCGGCCCGGGCGCAACCAATGCCTCC
GCCGGGCTCCATGTGGCACGGCAGGATTCGGTTCCCATGATCCTCTTCATCGGCCAGGTGCAGCGGGAGGCGCGCGAGCG
GGAGGCTTTCCAGGAGATCGAATACCGTCGCGCCTTCACCGAGGTCGCAAAATGGGTGGGCGAGATAGACGACCCGGCAC
GCATCCCCGAATTCGTCACTCGCGCCTTCGCCGTCGCGACGTCCGGCCGCCCCGGCCCAGTGGTCCTGACCTTGCCGGAG
GACATGCTGACACAGAGTGCCGAGGCCCCGGCCGCACGCGCCTACCAACCGGTGGAAAGCCACCCTGGAACCGGCCAAAT
CGCGCGCCTCGCGGAACTTCTCTCTTCAGCGAAAAGGCCGATCGCCATTCTCGGCGGCACGCGCTGGTCTGCCGAAGCCG
CGGCCGGGCTCAAGAGCTTCGCCGAACGCTGGCACCTCCCGGTCGGCTGCTCCTTCCGTCGGCAGATGCTTTTCGACCAC
CTGCACCCGAATTATGCGGGCGATGTCGGCATCGGCATCAATCCGGCACTGGCAGGAGAGATCAGGGAAGCCGATCTCAT
CCTCCTCATCGGCGGCCGGTTCTCGGAAATGCCCTCCTCCGGCTACACGCTGATCGACGTCCCCTACCCCAGGCAGACCC
TGGTGCATGTCCATCCGGATCCGGGGGAACTCGGCCGGGTCTACCGTCCGGACCTCGCGATTGCCGCGAGCCCGCAGGAC
TTCGTCGCGGCGCTCTCCGGCCTGACGCCTGCGGCCGAACCCAGCTGGTCCGCCCGCACAAGGGAAATGCATGCTGCCTA
TCTCAAATGGTCGACGCCGCCGGAAAAGGGACCGGGCGACGTTCAGATGGGCCCGATCGTCAACTGGCTGGAAGCGAATA
CGGGGCCGGAGACGATTTTTACCAACGGCGCGGGCAACTACGCGACCTGGCTCCATCGCTTCCACCGCTTCCGCCGTTAC
GGCACGCAGGCTGCTCCCGCTTCCGGTTCGATGGGCTACGGCCTGCCGGCCGCTGTCGCAGCCAAGCATCTGCATCCCGA
CCGCGAGGTGATCTGCTTTGCCGGAGACGGCTGTTTCCTCATGCACGGCCAGGAATTCGCGACGGCCGTCCGCTACGGAC
TCCCCCTTATCGTGCTCGTCATCAACAACAACATCTACGGCACGATCCGCATGCACCAGGAGCGCGAATATCCCGGCCGC
GTCAGTGCCACGGATCTGACGAACCCGGATTTTGCCGCACTTGCCCGCGCCTATGGCGGACATGGCGAAACGGTGGCGCG
CACCGAGGAGTTTGCGGACGCTTTCCTGCGTGCGCGTGAAAGCGGCAAGCCCGCCATTATCGAGGTCAAGCTCGATCCGG
AAGCGATCACGCCGACGCGGACGCTGAGCGAGATCCGCAAAGGATAG

Upstream 100 bases:

>100_bases
CATATGGCGAGAGCGGCAGGTTCACAAAACGGCGAGTATCCCGCCGGTATCGCCGGCGATCTGACATCGGCGAATCAGGA
CAGCATTGGGAGGTGCGGAC

Downstream 100 bases:

>100_bases
CAAGGGAAGAAGAGAGATTCCCGCGCCGCAGACGCGGCGCTGCTGGATTTCTGTGACAAGCACAGAAATGAGGGAAAGGG
GAGAGATGGCTTGCACAGCG

Product: thiamine pyrophosphate protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MKTGGQLVVEALVANGVKRISCVPGESYLAVLDALYDSDVEVVVCRQEGGAAMMADAWGRLTGEPGICMVTRGPGATNAS
AGLHVARQDSVPMILFIGQVQREAREREAFQEIEYRRAFTEVAKWVGEIDDPARIPEFVTRAFAVATSGRPGPVVLTLPE
DMLTQSAEAPAARAYQPVESHPGTGQIARLAELLSSAKRPIAILGGTRWSAEAAAGLKSFAERWHLPVGCSFRRQMLFDH
LHPNYAGDVGIGINPALAGEIREADLILLIGGRFSEMPSSGYTLIDVPYPRQTLVHVHPDPGELGRVYRPDLAIAASPQD
FVAALSGLTPAAEPSWSARTREMHAAYLKWSTPPEKGPGDVQMGPIVNWLEANTGPETIFTNGAGNYATWLHRFHRFRRY
GTQAAPASGSMGYGLPAAVAAKHLHPDREVICFAGDGCFLMHGQEFATAVRYGLPLIVLVINNNIYGTIRMHQEREYPGR
VSATDLTNPDFAALARAYGGHGETVARTEEFADAFLRARESGKPAIIEVKLDPEAITPTRTLSEIRKG

Sequences:

>Translated_548_residues
MKTGGQLVVEALVANGVKRISCVPGESYLAVLDALYDSDVEVVVCRQEGGAAMMADAWGRLTGEPGICMVTRGPGATNAS
AGLHVARQDSVPMILFIGQVQREAREREAFQEIEYRRAFTEVAKWVGEIDDPARIPEFVTRAFAVATSGRPGPVVLTLPE
DMLTQSAEAPAARAYQPVESHPGTGQIARLAELLSSAKRPIAILGGTRWSAEAAAGLKSFAERWHLPVGCSFRRQMLFDH
LHPNYAGDVGIGINPALAGEIREADLILLIGGRFSEMPSSGYTLIDVPYPRQTLVHVHPDPGELGRVYRPDLAIAASPQD
FVAALSGLTPAAEPSWSARTREMHAAYLKWSTPPEKGPGDVQMGPIVNWLEANTGPETIFTNGAGNYATWLHRFHRFRRY
GTQAAPASGSMGYGLPAAVAAKHLHPDREVICFAGDGCFLMHGQEFATAVRYGLPLIVLVINNNIYGTIRMHQEREYPGR
VSATDLTNPDFAALARAYGGHGETVARTEEFADAFLRARESGKPAIIEVKLDPEAITPTRTLSEIRKG
>Mature_548_residues
MKTGGQLVVEALVANGVKRISCVPGESYLAVLDALYDSDVEVVVCRQEGGAAMMADAWGRLTGEPGICMVTRGPGATNAS
AGLHVARQDSVPMILFIGQVQREAREREAFQEIEYRRAFTEVAKWVGEIDDPARIPEFVTRAFAVATSGRPGPVVLTLPE
DMLTQSAEAPAARAYQPVESHPGTGQIARLAELLSSAKRPIAILGGTRWSAEAAAGLKSFAERWHLPVGCSFRRQMLFDH
LHPNYAGDVGIGINPALAGEIREADLILLIGGRFSEMPSSGYTLIDVPYPRQTLVHVHPDPGELGRVYRPDLAIAASPQD
FVAALSGLTPAAEPSWSARTREMHAAYLKWSTPPEKGPGDVQMGPIVNWLEANTGPETIFTNGAGNYATWLHRFHRFRRY
GTQAAPASGSMGYGLPAAVAAKHLHPDREVICFAGDGCFLMHGQEFATAVRYGLPLIVLVINNNIYGTIRMHQEREYPGR
VSATDLTNPDFAALARAYGGHGETVARTEEFADAFLRARESGKPAIIEVKLDPEAITPTRTLSEIRKG

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=570, Percent_Identity=25.4385964912281, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI21361361, Length=585, Percent_Identity=26.1538461538462, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI87081685, Length=556, Percent_Identity=30.5755395683453, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI1790104, Length=549, Percent_Identity=29.143897996357, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1786717, Length=523, Percent_Identity=25.8126195028681, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1787096, Length=564, Percent_Identity=25.709219858156, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1788716, Length=579, Percent_Identity=26.0794473229706, Blast_Score=112, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17531301, Length=570, Percent_Identity=26.6666666666667, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17531299, Length=570, Percent_Identity=26.6666666666667, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17542570, Length=553, Percent_Identity=24.4122965641953, Blast_Score=101, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323755, Length=559, Percent_Identity=30.0536672629696, Blast_Score=223, Evalue=5e-59,
Organism=Saccharomyces cerevisiae, GI6323163, Length=492, Percent_Identity=24.1869918699187, Blast_Score=90, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6323073, Length=487, Percent_Identity=23.4086242299795, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19922626, Length=565, Percent_Identity=24.6017699115044, Blast_Score=136, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59281; Mature: 59281

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTGGQLVVEALVANGVKRISCVPGESYLAVLDALYDSDVEVVVCRQEGGAAMMADAWGR
CCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCEEEEEHHHHC
LTGEPGICMVTRGPGATNASAGLHVARQDSVPMILFIGQVQREAREREAFQEIEYRRAFT
CCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
EVAKWVGEIDDPARIPEFVTRAFAVATSGRPGPVVLTLPEDMLTQSAEAPAARAYQPVES
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHCCCCCCHHHCCCCHHC
HPGTGQIARLAELLSSAKRPIAILGGTRWSAEAAAGLKSFAERWHLPVGCSFRRQMLFDH
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LHPNYAGDVGIGINPALAGEIREADLILLIGGRFSEMPSSGYTLIDVPYPRQTLVHVHPD
CCCCCCCCCCCCCCCHHCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCEEEEECCC
PGELGRVYRPDLAIAASPQDFVAALSGLTPAAEPSWSARTREMHAAYLKWSTPPEKGPGD
CCCCCCEECCCEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHEEEEECCCCCCCCCC
VQMGPIVNWLEANTGPETIFTNGAGNYATWLHRFHRFRRYGTQAAPASGSMGYGLPAAVA
EECCCHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
AKHLHPDREVICFAGDGCFLMHGQEFATAVRYGLPLIVLVINNNIYGTIRMHQEREYPGR
HHHCCCCCEEEEEECCCEEEEECHHHHHHHHHCCCEEEEEECCCEEEEEEEECCCCCCCC
VSATDLTNPDFAALARAYGGHGETVARTEEFADAFLRARESGKPAIIEVKLDPEAITPTR
CCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHH
TLSEIRKG
HHHHHHCC
>Mature Secondary Structure
MKTGGQLVVEALVANGVKRISCVPGESYLAVLDALYDSDVEVVVCRQEGGAAMMADAWGR
CCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCEEEEEHHHHC
LTGEPGICMVTRGPGATNASAGLHVARQDSVPMILFIGQVQREAREREAFQEIEYRRAFT
CCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
EVAKWVGEIDDPARIPEFVTRAFAVATSGRPGPVVLTLPEDMLTQSAEAPAARAYQPVES
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHCCCCCCHHHCCCCHHC
HPGTGQIARLAELLSSAKRPIAILGGTRWSAEAAAGLKSFAERWHLPVGCSFRRQMLFDH
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LHPNYAGDVGIGINPALAGEIREADLILLIGGRFSEMPSSGYTLIDVPYPRQTLVHVHPD
CCCCCCCCCCCCCCCHHCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCEEEEECCC
PGELGRVYRPDLAIAASPQDFVAALSGLTPAAEPSWSARTREMHAAYLKWSTPPEKGPGD
CCCCCCEECCCEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHEEEEECCCCCCCCCC
VQMGPIVNWLEANTGPETIFTNGAGNYATWLHRFHRFRRYGTQAAPASGSMGYGLPAAVA
EECCCHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
AKHLHPDREVICFAGDGCFLMHGQEFATAVRYGLPLIVLVINNNIYGTIRMHQEREYPGR
HHHCCCCCEEEEEECCCEEEEECHHHHHHHHHCCCEEEEEECCCEEEEEEEECCCCCCCC
VSATDLTNPDFAALARAYGGHGETVARTEEFADAFLRARESGKPAIIEVKLDPEAITPTR
CCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHH
TLSEIRKG
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]