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The map label for this gene is amiD [H]

Identifier: 150397305

GI number: 150397305

Start: 2165276

End: 2166040

Strand: Reverse

Name: amiD [H]

Synonym: Smed_2104

Alternate gene names: 150397305

Gene position: 2166040-2165276 (Counterclockwise)

Preceding gene: 150397306

Following gene: 150397304

Centisome position: 57.27

GC content: 61.05

Gene sequence:

>765_bases
ATGACATCCAGCACCTGCGACTTCCCCGGCGCCCGTCTCGTGCCTTCGCCCAACCACGGCGAGCGCGCCGGTGGGCGCAG
GCCCGATATGATCATACTTCACTACACCGGCATGGAGACGGCGGCTTCTGCGCTCGATTGGCTCTGTCGCAAGGAAAGCC
AGGTCTCCTGCCATTACTTCGTCGATGAGGAAGGGCGCATTGCCCAGTTGGTGCCGGAAGAGCGCCGGGCCTGGCACGCG
GGCAAAAGCTTCTGGAAGGGTGAGACGGATATCAATTCCTGCTCGATCGGGATCGAGATCGCCAATGCCGGCCATCCGGG
CGGTCTGCCGGACTTTCCTAAGGCGCAGGTCGCGGCGGTCGCCGAATTATGTCTGGACTGTGGCGAACGCTGGGAAATTG
CCCCCGAGAGGGTGCTTGCTCACAGCGATATTGCGCCGATTCGCAAGGTTGATCCTGGAGAAAATTTTCCTTGGGATGTG
TTGTTTCGTCGCGGGGTCGGACACTGGGTGGAACCCGCGCCGGTGAGGGGCGGGCGCTTCTTCCAGTGCGGCGACTGCGG
GCAACCGGTGGAAGCACTTCAGTCGATGCTCCTGCTTTACGGCTACGGCGTTGAAATAACAGGAGAATTCTGCCAGAAAA
CCGAAGGCGCCGTCGCGGCGTTTCAGCGCCATTTCCGGCAATCGCGAGTGGATGGAATTGCCGACGTTTCCACCATCGAT
ACCCTGCACCGTCTGCTGTCCGCGCTGCCGAATCTTAACGACTGA

Upstream 100 bases:

>100_bases
TGGCGCGTGGCGTGCCGGAGGAGTTCCACGCGATCGCCAACGATCGCATGGCCGTGCTCAACGCGGCTTACGAGGCAATT
GAAAGAGAACGCCGGGCCGC

Downstream 100 bases:

>100_bases
ACGACGATTTTCCTCCGGCCTAATTTAGCGTTCGGTTGATTTTCGTGCACGATTTTGTCGTCCTGCCACATGTTTACCAT
GATAGCCCGGTCATTTCCGC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTSSTCDFPGARLVPSPNHGERAGGRRPDMIILHYTGMETAASALDWLCRKESQVSCHYFVDEEGRIAQLVPEERRAWHA
GKSFWKGETDINSCSIGIEIANAGHPGGLPDFPKAQVAAVAELCLDCGERWEIAPERVLAHSDIAPIRKVDPGENFPWDV
LFRRGVGHWVEPAPVRGGRFFQCGDCGQPVEALQSMLLLYGYGVEITGEFCQKTEGAVAAFQRHFRQSRVDGIADVSTID
TLHRLLSALPNLND

Sequences:

>Translated_254_residues
MTSSTCDFPGARLVPSPNHGERAGGRRPDMIILHYTGMETAASALDWLCRKESQVSCHYFVDEEGRIAQLVPEERRAWHA
GKSFWKGETDINSCSIGIEIANAGHPGGLPDFPKAQVAAVAELCLDCGERWEIAPERVLAHSDIAPIRKVDPGENFPWDV
LFRRGVGHWVEPAPVRGGRFFQCGDCGQPVEALQSMLLLYGYGVEITGEFCQKTEGAVAAFQRHFRQSRVDGIADVSTID
TLHRLLSALPNLND
>Mature_253_residues
TSSTCDFPGARLVPSPNHGERAGGRRPDMIILHYTGMETAASALDWLCRKESQVSCHYFVDEEGRIAQLVPEERRAWHAG
KSFWKGETDINSCSIGIEIANAGHPGGLPDFPKAQVAAVAELCLDCGERWEIAPERVLAHSDIAPIRKVDPGENFPWDVL
FRRGVGHWVEPAPVRGGRFFQCGDCGQPVEALQSMLLLYGYGVEITGEFCQKTEGAVAAFQRHFRQSRVDGIADVSTIDT
LHRLLSALPNLND

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=239, Percent_Identity=37.2384937238494, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1786300, Length=166, Percent_Identity=31.3253012048193, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 27970; Mature: 27839

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSTCDFPGARLVPSPNHGERAGGRRPDMIILHYTGMETAASALDWLCRKESQVSCHYF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEEE
VDEEGRIAQLVPEERRAWHAGKSFWKGETDINSCSIGIEIANAGHPGGLPDFPKAQVAAV
ECCCCCEECCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHH
AELCLDCGERWEIAPERVLAHSDIAPIRKVDPGENFPWDVLFRRGVGHWVEPAPVRGGRF
HHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCE
FQCGDCGQPVEALQSMLLLYGYGVEITGEFCQKTEGAVAAFQRHFRQSRVDGIADVSTID
EECCCCCCHHHHHHHHHHHHCCCCEEEHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHH
TLHRLLSALPNLND
HHHHHHHHCCCCCC
>Mature Secondary Structure 
TSSTCDFPGARLVPSPNHGERAGGRRPDMIILHYTGMETAASALDWLCRKESQVSCHYF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEEE
VDEEGRIAQLVPEERRAWHAGKSFWKGETDINSCSIGIEIANAGHPGGLPDFPKAQVAAV
ECCCCCEECCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHH
AELCLDCGERWEIAPERVLAHSDIAPIRKVDPGENFPWDVLFRRGVGHWVEPAPVRGGRF
HHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCE
FQCGDCGQPVEALQSMLLLYGYGVEITGEFCQKTEGAVAAFQRHFRQSRVDGIADVSTID
EECCCCCCHHHHHHHHHHHHCCCCEEEHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHH
TLHRLLSALPNLND
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]