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The map label for this gene is glyA [H]

Identifier: 150397297

GI number: 150397297

Start: 2156225

End: 2157502

Strand: Reverse

Name: glyA [H]

Synonym: Smed_2096

Alternate gene names: 150397297

Gene position: 2157502-2156225 (Counterclockwise)

Preceding gene: 150397303

Following gene: 150397296

Centisome position: 57.05

GC content: 61.42

Gene sequence:

>1278_bases
ATGACCGAATACACGAAGGCTTATTTCAATGCGCCGGTTCACGAACGCGACCCATTGGTCGCGCAGGCCATCGACAATGA
ACGCAAGCGCCAGCAGGACCAGATCGAACTCATCGCTTCGGAGAACATCGTCAGCCGGGCCGTTCTCGATGCGCTTGGCC
ACGAGATGACGAACAAGACCCTGGAAGGTTACCCGGGAAACCGCTTCCACGGTGGAGGCCAGTTCGTCGATGTGGTGGAG
CAGGCCGCAATCGACCGGGCGAAGCAGCTTTTCGGCTGCGCATATGCCAATGTCCAGCCGCATTCGGGCACTCAGGCAAA
CCTCGCCGTATTCTTCCTGCTCCTGACGCCGGGGGACAAGGTTCTTTCGCTTGACCTTGCGGCAGGCGGTCACCTGTCGC
ACGGCATGAAGGGCAATCTTTCGGGCCGCTGGTTCGAACCCCACAACTACAATGTGAACCCGGAAACCGAAGTCATCGAT
TATGACGAACTGGAGCGGATCGCCGAAGAGGTGCGTCCGACACTCCTGATCACCGGCGGCTCGGCCTATCCGCGCGAACT
CGATTTCGAACGCATGGGCAATATTGCAAAAAAGGTTGGCGCCTGGTTCCTGGTAGACATGGCGCATATCGCCGGTCTCG
TGGCAGGCGGGGTCCATCCTTCGCCGTTCCCGCACGCCGATATCGTCACCTGCACGACGACCAAGACGCTGCGCGGCCCG
CGCGGGGGACTGATCCTCACCAACAACGAAGCCTGGTTCAAGAAGCTCCAGTCCGCGGTGTTCCCGGGGGTCCAGGGATC
GCTCCACAGCAATGTGCTGGCGGCCAAGGCGATCTGCCTCGGTGAGGCGCTTCGCGACGATTTCAAGGTCTATGCGGCGC
AAGTGAAAACCAATGCGCGGGTTCTCGCCGATGTCCTCATGGCCCGTGGAGTACGGGTCGTCTCCGGCGGCACGGACACC
CACATCGTACTTGTCGACCTGTCGAGCAAGGGCTTGATCGGCAAGCAGGCCGAGGATCTGCTGGCCCGTGCCAACATCAC
GGCCAACAAGAACCCGATCCCGAACGACAGCCCGCGTCCGCCGGAATGGTTGGGTATGCGCCTCGGCGTCTCCGCGGCCA
CGACACGCGGCATGAAGGAAGACGAATTCCGAACGCTCGGCACCATCATCGCAGACCTCATCGAGGCGGAAGCTGCCGGC
AATGCCGACCTTAGCGTCGAGGCTGCGAAGACGAAGGTGGCTGAACTGACGGCTGCCTTTCCCGTCTACGGTCACTGA

Upstream 100 bases:

>100_bases
CAGATCAGAACATATGTGCAAATATTATTAACTTTTTCTGGATCGATGCGGCCGCTAGCCTTCCTCGAGTTCTCATCATC
TTTCCGTCCGGAGACAGGTC

Downstream 100 bases:

>100_bases
ACGCGGGCGATAGCCGTACAGGCGGAGCGGGATGAGGAAAAGTGTGCTCGGTTGTCCGCCCGCATCCCGCTCTAACTTGC
TGCAGCCGGTGCGCCCGGTC

Product: glycine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTEYTKAYFNAPVHERDPLVAQAIDNERKRQQDQIELIASENIVSRAVLDALGHEMTNKTLEGYPGNRFHGGGQFVDVVE
QAAIDRAKQLFGCAYANVQPHSGTQANLAVFFLLLTPGDKVLSLDLAAGGHLSHGMKGNLSGRWFEPHNYNVNPETEVID
YDELERIAEEVRPTLLITGGSAYPRELDFERMGNIAKKVGAWFLVDMAHIAGLVAGGVHPSPFPHADIVTCTTTKTLRGP
RGGLILTNNEAWFKKLQSAVFPGVQGSLHSNVLAAKAICLGEALRDDFKVYAAQVKTNARVLADVLMARGVRVVSGGTDT
HIVLVDLSSKGLIGKQAEDLLARANITANKNPIPNDSPRPPEWLGMRLGVSAATTRGMKEDEFRTLGTIIADLIEAEAAG
NADLSVEAAKTKVAELTAAFPVYGH

Sequences:

>Translated_425_residues
MTEYTKAYFNAPVHERDPLVAQAIDNERKRQQDQIELIASENIVSRAVLDALGHEMTNKTLEGYPGNRFHGGGQFVDVVE
QAAIDRAKQLFGCAYANVQPHSGTQANLAVFFLLLTPGDKVLSLDLAAGGHLSHGMKGNLSGRWFEPHNYNVNPETEVID
YDELERIAEEVRPTLLITGGSAYPRELDFERMGNIAKKVGAWFLVDMAHIAGLVAGGVHPSPFPHADIVTCTTTKTLRGP
RGGLILTNNEAWFKKLQSAVFPGVQGSLHSNVLAAKAICLGEALRDDFKVYAAQVKTNARVLADVLMARGVRVVSGGTDT
HIVLVDLSSKGLIGKQAEDLLARANITANKNPIPNDSPRPPEWLGMRLGVSAATTRGMKEDEFRTLGTIIADLIEAEAAG
NADLSVEAAKTKVAELTAAFPVYGH
>Mature_424_residues
TEYTKAYFNAPVHERDPLVAQAIDNERKRQQDQIELIASENIVSRAVLDALGHEMTNKTLEGYPGNRFHGGGQFVDVVEQ
AAIDRAKQLFGCAYANVQPHSGTQANLAVFFLLLTPGDKVLSLDLAAGGHLSHGMKGNLSGRWFEPHNYNVNPETEVIDY
DELERIAEEVRPTLLITGGSAYPRELDFERMGNIAKKVGAWFLVDMAHIAGLVAGGVHPSPFPHADIVTCTTTKTLRGPR
GGLILTNNEAWFKKLQSAVFPGVQGSLHSNVLAAKAICLGEALRDDFKVYAAQVKTNARVLADVLMARGVRVVSGGTDTH
IVLVDLSSKGLIGKQAEDLLARANITANKNPIPNDSPRPPEWLGMRLGVSAATTRGMKEDEFRTLGTIIADLIEAEAAGN
ADLSVEAAKTKVAELTAAFPVYGH

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI261862352, Length=430, Percent_Identity=43.953488372093, Blast_Score=321, Evalue=9e-88,
Organism=Homo sapiens, GI261862350, Length=430, Percent_Identity=43.953488372093, Blast_Score=321, Evalue=9e-88,
Organism=Homo sapiens, GI261862348, Length=430, Percent_Identity=43.953488372093, Blast_Score=321, Evalue=9e-88,
Organism=Homo sapiens, GI19923315, Length=430, Percent_Identity=43.953488372093, Blast_Score=320, Evalue=1e-87,
Organism=Homo sapiens, GI22547186, Length=432, Percent_Identity=42.3611111111111, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI261862346, Length=430, Percent_Identity=42.7906976744186, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI22547189, Length=418, Percent_Identity=41.6267942583732, Blast_Score=283, Evalue=2e-76,
Organism=Escherichia coli, GI1788902, Length=413, Percent_Identity=50.363196125908, Blast_Score=388, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI25144732, Length=423, Percent_Identity=43.7352245862884, Blast_Score=324, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI25144729, Length=423, Percent_Identity=43.7352245862884, Blast_Score=324, Evalue=6e-89,
Organism=Saccharomyces cerevisiae, GI6319739, Length=414, Percent_Identity=40.5797101449275, Blast_Score=306, Evalue=3e-84,
Organism=Saccharomyces cerevisiae, GI6323087, Length=422, Percent_Identity=42.654028436019, Blast_Score=306, Evalue=4e-84,
Organism=Drosophila melanogaster, GI24640005, Length=459, Percent_Identity=40.0871459694989, Blast_Score=324, Evalue=9e-89,
Organism=Drosophila melanogaster, GI221329721, Length=459, Percent_Identity=40.0871459694989, Blast_Score=323, Evalue=1e-88,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45906; Mature: 45775

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS01034 GLYCOSYL_HYDROL_F16

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEYTKAYFNAPVHERDPLVAQAIDNERKRQQDQIELIASENIVSRAVLDALGHEMTNKT
CCCHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCH
LEGYPGNRFHGGGQFVDVVEQAAIDRAKQLFGCAYANVQPHSGTQANLAVFFLLLTPGDK
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCC
VLSLDLAAGGHLSHGMKGNLSGRWFEPHNYNVNPETEVIDYDELERIAEEVRPTLLITGG
EEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEECHHHHHHHHHHCCCEEEEECC
SAYPRELDFERMGNIAKKVGAWFLVDMAHIAGLVAGGVHPSPFPHADIVTCTTTKTLRGP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHCCCC
RGGLILTNNEAWFKKLQSAVFPGVQGSLHSNVLAAKAICLGEALRDDFKVYAAQVKTNAR
CCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
VLADVLMARGVRVVSGGTDTHIVLVDLSSKGLIGKQAEDLLARANITANKNPIPNDSPRP
HHHHHHHHCCEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
PEWLGMRLGVSAATTRGMKEDEFRTLGTIIADLIEAEAAGNADLSVEAAKTKVAELTAAF
HHHHCEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHCC
PVYGH
CCCCC
>Mature Secondary Structure 
TEYTKAYFNAPVHERDPLVAQAIDNERKRQQDQIELIASENIVSRAVLDALGHEMTNKT
CCHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCH
LEGYPGNRFHGGGQFVDVVEQAAIDRAKQLFGCAYANVQPHSGTQANLAVFFLLLTPGDK
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCC
VLSLDLAAGGHLSHGMKGNLSGRWFEPHNYNVNPETEVIDYDELERIAEEVRPTLLITGG
EEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEECHHHHHHHHHHCCCEEEEECC
SAYPRELDFERMGNIAKKVGAWFLVDMAHIAGLVAGGVHPSPFPHADIVTCTTTKTLRGP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHCCCC
RGGLILTNNEAWFKKLQSAVFPGVQGSLHSNVLAAKAICLGEALRDDFKVYAAQVKTNAR
CCCEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
VLADVLMARGVRVVSGGTDTHIVLVDLSSKGLIGKQAEDLLARANITANKNPIPNDSPRP
HHHHHHHHCCEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
PEWLGMRLGVSAATTRGMKEDEFRTLGTIIADLIEAEAAGNADLSVEAAKTKVAELTAAF
HHHHCEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHCC
PVYGH
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA