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The map label for this gene is mraW [H]

Identifier: 150397291

GI number: 150397291

Start: 2149127

End: 2150215

Strand: Reverse

Name: mraW [H]

Synonym: Smed_2090

Alternate gene names: 150397291

Gene position: 2150215-2149127 (Counterclockwise)

Preceding gene: 150397292

Following gene: 150397290

Centisome position: 56.86

GC content: 62.17

Gene sequence:

>1089_bases
ATGAAATCAGAGCAGTTCCGCGATTTTGAGAAAAGCGGACATGCTCTGGAGTGGAGAGGCGGGATGGTGACGGATCAAGG
CGGCAGGACTTCTGAAGCCGATGGCGGACCGGTTCGTCACATTCCCGTCCTCCTCGAGGAAGTTCTAGCAGCTCTTGAAC
CCGCTCCGGGCAAAGTTATTCTCGATGGCACGTTCGGTGCCGGCGGTTACGCGTCCGCCATCCTCGATGCAGGTGCCGAG
GTCATCGCCCTGGACCGGGATCCGACGGCGATCGCCGCCGGGCAGTCGATGGTAAGCGCCAGCGGCGGCCGGCTCAGCCT
TATCCATTCCCGTTTTTCCGGCCTCGCCGAGCATGCGCCCGCCGAGGGTCTCGACGGCGTCGTGCTGGACGTCGGCGTTT
CGTCGATGCAGATAGACGAGGCGGAGCGTGGCTTTTCTTTTCAGAAGAAGGGGCCGCTCGACATGCGCATGTCGGCCGCC
GGCGTCTCCGCAGCCGATGTCGTCAATCGCGCGAAGGTCTCGGATCTTATTCGCATCTTCGGCTTTCTCGGCGAGGAGAA
GCAGGCCGGACGAATTGCGCGTACCATCGAAAAGCGACGCGCCGAGGCTCCGTTCGAGACCACGCGCGATCTCGCCAATC
TTATCGAGACGGTGACGCCGCGTAAGGCGAAAGATAAGATTCACCCGGCAACGCGCGTCTTCCAGGCGCTGCGCATCTTC
GTCAATGACGAGCTCGGCGAACTTGCAGCTGCGCTCTTTGCGGCCGAACGGGCGCTGAAGCCCGGCGGTAGACTTGTTGT
CGTCTCCTTTCATTCACTCGAGGACAGGATCGTTAAGACGTTTTTCCAGGATCGCTCCGGCAAGGCGGGCGGTTCCCGCC
ATTTGCCGTTGGTGACGGCGCGCGCCGCAACCTTCGCGCCAGTCGGCAAGCCTATGGTATCGGCGAGCGATGAAGAGGCG
TCACGCAATCCGCGTGCCCGGTCCGCGAAGCTGAGAGCCGGAATACGAACAGCAGCTCCGTCGCCGGGTGCCGATTTGTC
CATTTTCAACCTGCCGGAACTGGCAAGCCTTGCAAGACTGGGGGGCTAG

Upstream 100 bases:

>100_bases
CGGAGGCCAGGGAAGGGCGCAAGCAACGGGGGCTGCGTTCGCAATAGAGCATTTCCAGGAAAACTGCGCAGCAGTTTTCT
GTCCGGAATTGCGGGGAAAC

Downstream 100 bases:

>100_bases
GAGACGATGCTCAGAACGCTCGATGTTGTCCTGATCGTCATCATGACGGCGGCCGCAACGGTCACCTATACGATCAAGCA
TAAGGCTGAGAACAAGCTTG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MKSEQFRDFEKSGHALEWRGGMVTDQGGRTSEADGGPVRHIPVLLEEVLAALEPAPGKVILDGTFGAGGYASAILDAGAE
VIALDRDPTAIAAGQSMVSASGGRLSLIHSRFSGLAEHAPAEGLDGVVLDVGVSSMQIDEAERGFSFQKKGPLDMRMSAA
GVSAADVVNRAKVSDLIRIFGFLGEEKQAGRIARTIEKRRAEAPFETTRDLANLIETVTPRKAKDKIHPATRVFQALRIF
VNDELGELAAALFAAERALKPGGRLVVVSFHSLEDRIVKTFFQDRSGKAGGSRHLPLVTARAATFAPVGKPMVSASDEEA
SRNPRARSAKLRAGIRTAAPSPGADLSIFNLPELASLARLGG

Sequences:

>Translated_362_residues
MKSEQFRDFEKSGHALEWRGGMVTDQGGRTSEADGGPVRHIPVLLEEVLAALEPAPGKVILDGTFGAGGYASAILDAGAE
VIALDRDPTAIAAGQSMVSASGGRLSLIHSRFSGLAEHAPAEGLDGVVLDVGVSSMQIDEAERGFSFQKKGPLDMRMSAA
GVSAADVVNRAKVSDLIRIFGFLGEEKQAGRIARTIEKRRAEAPFETTRDLANLIETVTPRKAKDKIHPATRVFQALRIF
VNDELGELAAALFAAERALKPGGRLVVVSFHSLEDRIVKTFFQDRSGKAGGSRHLPLVTARAATFAPVGKPMVSASDEEA
SRNPRARSAKLRAGIRTAAPSPGADLSIFNLPELASLARLGG
>Mature_362_residues
MKSEQFRDFEKSGHALEWRGGMVTDQGGRTSEADGGPVRHIPVLLEEVLAALEPAPGKVILDGTFGAGGYASAILDAGAE
VIALDRDPTAIAAGQSMVSASGGRLSLIHSRFSGLAEHAPAEGLDGVVLDVGVSSMQIDEAERGFSFQKKGPLDMRMSAA
GVSAADVVNRAKVSDLIRIFGFLGEEKQAGRIARTIEKRRAEAPFETTRDLANLIETVTPRKAKDKIHPATRVFQALRIF
VNDELGELAAALFAAERALKPGGRLVVVSFHSLEDRIVKTFFQDRSGKAGGSRHLPLVTARAATFAPVGKPMVSASDEEA
SRNPRARSAKLRAGIRTAAPSPGADLSIFNLPELASLARLGG

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=335, Percent_Identity=41.1940298507463, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI165377202, Length=196, Percent_Identity=40.3061224489796, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1786270, Length=314, Percent_Identity=43.9490445859873, Blast_Score=225, Evalue=3e-60,
Organism=Drosophila melanogaster, GI62472493, Length=341, Percent_Identity=39.5894428152493, Blast_Score=207, Evalue=9e-54,
Organism=Drosophila melanogaster, GI28571637, Length=341, Percent_Identity=39.5894428152493, Blast_Score=207, Evalue=9e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 38295; Mature: 38295

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSEQFRDFEKSGHALEWRGGMVTDQGGRTSEADGGPVRHIPVLLEEVLAALEPAPGKVI
CCCHHHHHHHHCCCEEEECCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEE
LDGTFGAGGYASAILDAGAEVIALDRDPTAIAAGQSMVSASGGRLSLIHSRFSGLAEHAP
EECCCCCCHHHHHHHHCCCEEEEECCCCCHHHCCCHHHHCCCCEEEHHHHHHHHHHHHCC
AEGLDGVVLDVGVSSMQIDEAERGFSFQKKGPLDMRMSAAGVSAADVVNRAKVSDLIRIF
CCCCCCEEEECCCCHHEHHHHHCCCCCCCCCCCCEEEHHCCCCHHHHHHHHHHHHHHHHH
GFLGEEKQAGRIARTIEKRRAEAPFETTRDLANLIETVTPRKAKDKIHPATRVFQALRIF
HHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHH
VNDELGELAAALFAAERALKPGGRLVVVSFHSLEDRIVKTFFQDRSGKAGGSRHLPLVTA
HCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
RAATFAPVGKPMVSASDEEASRNPRARSAKLRAGIRTAAPSPGADLSIFNLPELASLARL
CCHHHCCCCCCCCCCCCHHHHCCCCHHHHHHHHCHHHCCCCCCCCEEEECCHHHHHHHHC
GG
CC
>Mature Secondary Structure
MKSEQFRDFEKSGHALEWRGGMVTDQGGRTSEADGGPVRHIPVLLEEVLAALEPAPGKVI
CCCHHHHHHHHCCCEEEECCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEE
LDGTFGAGGYASAILDAGAEVIALDRDPTAIAAGQSMVSASGGRLSLIHSRFSGLAEHAP
EECCCCCCHHHHHHHHCCCEEEEECCCCCHHHCCCHHHHCCCCEEEHHHHHHHHHHHHCC
AEGLDGVVLDVGVSSMQIDEAERGFSFQKKGPLDMRMSAAGVSAADVVNRAKVSDLIRIF
CCCCCCEEEECCCCHHEHHHHHCCCCCCCCCCCCEEEHHCCCCHHHHHHHHHHHHHHHHH
GFLGEEKQAGRIARTIEKRRAEAPFETTRDLANLIETVTPRKAKDKIHPATRVFQALRIF
HHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHH
VNDELGELAAALFAAERALKPGGRLVVVSFHSLEDRIVKTFFQDRSGKAGGSRHLPLVTA
HCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
RAATFAPVGKPMVSASDEEASRNPRARSAKLRAGIRTAAPSPGADLSIFNLPELASLARL
CCHHHCCCCCCCCCCCCHHHHCCCCHHHHHHHHCHHHCCCCCCCCEEEECCHHHHHHHHC
GG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]