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The map label for this gene is yneF [H]

Identifier: 150397247

GI number: 150397247

Start: 2097741

End: 2099057

Strand: Direct

Name: yneF [H]

Synonym: Smed_2046

Alternate gene names: 150397247

Gene position: 2097741-2099057 (Clockwise)

Preceding gene: 150397244

Following gene: 150397248

Centisome position: 55.47

GC content: 63.1

Gene sequence:

>1317_bases
ATGCGCGGCCGCTACCAATGGCCTTGCATACTGGCTGAAACGCAAGCCGCACTTTATTTTAGCACGACAGGCCGTTTCGA
AATGAGCTTTCTCTCCGCCATACCCGCCATCGCACTTCTTGCCATGCTTCCCGTCCTCGCTTCTCTGCGGCGCATGCCGG
TTGCCGGTGTTCGGGAAATCGCGCTCGGCTGCGTATTGTCGGCGGCGTCGGCAGCGGCGCTGACCGTTGCGACGGCCATG
CAGGCGCCGATCGCTGCAATGTTCGGCTATGCAGGTCTGGTCGTCACCCTCTTCTTTGTCCTGCGAGGCCTTCAACGGTT
CTTCGCGCTATCGATGCCAACCCCGCTTTTTCTGGTACTGGCGTTCTCGAGCGGCGTGGTCGGGTTCATCATTGCGCTTG
GCGGCAATGGAAGTGCCGCCGCCACCATCATCGTCGTTGCGGGCGTGACCGCGCTCCCACTTCTTATCCTCGGCCTGGCC
GCGGCCAATCGCTGGCCAAAGGAAATGCCGGTGATCCAGGTCGTCATTATCGGCTCCGTCGTCGTGGTCTTCACCGCCTT
GGCTCATATCCTTGGCCCGTCGTCCCACTTCACGGCCGAGCGATCGGGATCGCCCGGCTTCATTCTGTTCGATTTCTTAG
TCGTCACCATGCAGGCGCTTTGTCTTCCGCTTGTCTTCATTGCCGTGATCCTCACGATGCAGGCGCGCATCATCGCCGCG
TTAAGAGCCGCGATCGCTCGGGACGGGCTGACCGGGGCGCTGTCGCGCGGAGCACTCATGGAAGAAGGCGAGCAGATCTT
CGCCGAGTGCCTGACCCGCCGAAGGCCGGCTGCCTTCCTGTTGCTCGACCTCGATTTTTTCAAGCAGATCAATGATCAGT
ACGGGCACGCCTGCGGCGACATGGCACTCGCGCACTTTGCCGACACCGTCTCGACGTTCCTCGCAGGTCGGGGCGTTCTC
GGACGGATCGGAGGAGAGGAATTCGGCATCGTCCTGCCGGACCACACTGAAGAGCAGGCAAAGGCGCTTGCCGAAGAGAT
CTGCCGCATCGTGCGCGAGACGCCTGCCGGCCGCTCTCATCAGCGCATCCGTTTGACCGTGAGCATAGGGATCGCAGCCG
CCACCGCGCACGAGACGATTGCGGATGTGATGATCCGCGCAGACCTGGCCCTCTACGACTCCAAGGCGGACGGTCGCGAT
CGTTGCACGATCGCCAGGGATCGCCGGATTGACTCGAGCGCCCGCGCCCTTGCAGCAGCCGCCGCGCAATTGCGGGAAGC
GCGCGCCGGCCGGCCGGAATTGCGCCACTCGGCGTGA

Upstream 100 bases:

>100_bases
CCTGCGTCATGACATTCGCTCCGTGAAAGCGTGCACCGACAACCACAACTATGGAGAGAGTGTTCGTTCAAATTGCAGCG
AATTCCGCCAGCTTTCGTTA

Downstream 100 bases:

>100_bases
CCGTCCGCACGGCGTCTCCAGAACCGGGCGTCGACGGGGTGGCGGCCGGGAGCGTTGCATTGTAGCATCCGCCGCTTGTC
TGGACTTTCCAGCTCATTGG

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MRGRYQWPCILAETQAALYFSTTGRFEMSFLSAIPAIALLAMLPVLASLRRMPVAGVREIALGCVLSAASAAALTVATAM
QAPIAAMFGYAGLVVTLFFVLRGLQRFFALSMPTPLFLVLAFSSGVVGFIIALGGNGSAAATIIVVAGVTALPLLILGLA
AANRWPKEMPVIQVVIIGSVVVVFTALAHILGPSSHFTAERSGSPGFILFDFLVVTMQALCLPLVFIAVILTMQARIIAA
LRAAIARDGLTGALSRGALMEEGEQIFAECLTRRRPAAFLLLDLDFFKQINDQYGHACGDMALAHFADTVSTFLAGRGVL
GRIGGEEFGIVLPDHTEEQAKALAEEICRIVRETPAGRSHQRIRLTVSIGIAAATAHETIADVMIRADLALYDSKADGRD
RCTIARDRRIDSSARALAAAAAQLREARAGRPELRHSA

Sequences:

>Translated_438_residues
MRGRYQWPCILAETQAALYFSTTGRFEMSFLSAIPAIALLAMLPVLASLRRMPVAGVREIALGCVLSAASAAALTVATAM
QAPIAAMFGYAGLVVTLFFVLRGLQRFFALSMPTPLFLVLAFSSGVVGFIIALGGNGSAAATIIVVAGVTALPLLILGLA
AANRWPKEMPVIQVVIIGSVVVVFTALAHILGPSSHFTAERSGSPGFILFDFLVVTMQALCLPLVFIAVILTMQARIIAA
LRAAIARDGLTGALSRGALMEEGEQIFAECLTRRRPAAFLLLDLDFFKQINDQYGHACGDMALAHFADTVSTFLAGRGVL
GRIGGEEFGIVLPDHTEEQAKALAEEICRIVRETPAGRSHQRIRLTVSIGIAAATAHETIADVMIRADLALYDSKADGRD
RCTIARDRRIDSSARALAAAAAQLREARAGRPELRHSA
>Mature_438_residues
MRGRYQWPCILAETQAALYFSTTGRFEMSFLSAIPAIALLAMLPVLASLRRMPVAGVREIALGCVLSAASAAALTVATAM
QAPIAAMFGYAGLVVTLFFVLRGLQRFFALSMPTPLFLVLAFSSGVVGFIIALGGNGSAAATIIVVAGVTALPLLILGLA
AANRWPKEMPVIQVVIIGSVVVVFTALAHILGPSSHFTAERSGSPGFILFDFLVVTMQALCLPLVFIAVILTMQARIIAA
LRAAIARDGLTGALSRGALMEEGEQIFAECLTRRRPAAFLLLDLDFFKQINDQYGHACGDMALAHFADTVSTFLAGRGVL
GRIGGEEFGIVLPDHTEEQAKALAEEICRIVRETPAGRSHQRIRLTVSIGIAAATAHETIADVMIRADLALYDSKADGRD
RCTIARDRRIDSSARALAAAAAQLREARAGRPELRHSA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=275, Percent_Identity=32.3636363636364, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1787262, Length=187, Percent_Identity=31.5508021390374, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=34.5238095238095, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=36.046511627907, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788381, Length=211, Percent_Identity=29.8578199052133, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786584, Length=201, Percent_Identity=30.3482587064677, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1787816, Length=165, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788956, Length=170, Percent_Identity=32.9411764705882, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081974, Length=195, Percent_Identity=27.1794871794872, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=29.4478527607362, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI145693134, Length=200, Percent_Identity=28.5, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081977, Length=164, Percent_Identity=34.1463414634146, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787541, Length=170, Percent_Identity=29.4117647058824, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 46556; Mature: 46556

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGRYQWPCILAETQAALYFSTTGRFEMSFLSAIPAIALLAMLPVLASLRRMPVAGVREI
CCCCCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ALGCVLSAASAAALTVATAMQAPIAAMFGYAGLVVTLFFVLRGLQRFFALSMPTPLFLVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AFSSGVVGFIIALGGNGSAAATIIVVAGVTALPLLILGLAAANRWPKEMPVIQVVIIGSV
HHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
VVVFTALAHILGPSSHFTAERSGSPGFILFDFLVVTMQALCLPLVFIAVILTMQARIIAA
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRAAIARDGLTGALSRGALMEEGEQIFAECLTRRRPAAFLLLDLDFFKQINDQYGHACGD
HHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCHHHHH
MALAHFADTVSTFLAGRGVLGRIGGEEFGIVLPDHTEEQAKALAEEICRIVRETPAGRSH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCC
QRIRLTVSIGIAAATAHETIADVMIRADLALYDSKADGRDRCTIARDRRIDSSARALAAA
CEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHCCCCCHHHHHHHHH
AAQLREARAGRPELRHSA
HHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRGRYQWPCILAETQAALYFSTTGRFEMSFLSAIPAIALLAMLPVLASLRRMPVAGVREI
CCCCCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ALGCVLSAASAAALTVATAMQAPIAAMFGYAGLVVTLFFVLRGLQRFFALSMPTPLFLVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AFSSGVVGFIIALGGNGSAAATIIVVAGVTALPLLILGLAAANRWPKEMPVIQVVIIGSV
HHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
VVVFTALAHILGPSSHFTAERSGSPGFILFDFLVVTMQALCLPLVFIAVILTMQARIIAA
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRAAIARDGLTGALSRGALMEEGEQIFAECLTRRRPAAFLLLDLDFFKQINDQYGHACGD
HHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCHHHHH
MALAHFADTVSTFLAGRGVLGRIGGEEFGIVLPDHTEEQAKALAEEICRIVRETPAGRSH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCC
QRIRLTVSIGIAAATAHETIADVMIRADLALYDSKADGRDRCTIARDRRIDSSARALAAA
CEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHCCCCCHHHHHHHHH
AAQLREARAGRPELRHSA
HHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]